Standard name | |
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Human Ortholog | |
Description | Ubiquitin-specific protease; specifically disassembles unanchored ubiquitin chains; involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; similar to human isopeptidase T |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 4 |
Bud | 0 | 0 | 2 | 1 | 0 | 1 | 3 | 2 | 1 | 10 | 11 | 6 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 1 | 4 | 9 | 10 |
Bud Neck | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 5 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 4 | 7 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 4 | 5 | 5 | 10 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 32 | 74 | 55 | 17 | 31 | 37 | 41 | 53 | 25 | 33 | 27 | 18 | 53 | 65 | 74 | 61 | 50 | 44 | 29 | 70 | 61 | 39 | 57 | 93 |
Endoplasmic Reticulum | 1 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 2 | 13 | 0 | 0 | 0 | 0 | 1 | 4 |
Endosome | 0 | 2 | 3 | 0 | 3 | 4 | 1 | 3 | 1 | 0 | 0 | 0 | 4 | 9 | 11 | 4 | 7 | 3 | 1 | 4 | 0 | 0 | 1 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 3 | 0 | 1 | 0 | 0 | 0 | 0 |
Mitochondria | 22 | 36 | 6 | 18 | 3 | 63 | 100 | 88 | 82 | 128 | 193 | 118 | 8 | 2 | 13 | 3 | 2 | 5 | 2 | 6 | 5 | 2 | 8 | 9 |
Nucleus | 34 | 57 | 31 | 50 | 77 | 120 | 165 | 191 | 85 | 108 | 156 | 147 | 47 | 42 | 56 | 49 | 56 | 50 | 58 | 113 | 55 | 46 | 64 | 61 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 8 | 0 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 7 | 3 | 2 | 3 | 5 |
Vac/Vac Membrane | 46 | 97 | 51 | 13 | 21 | 22 | 20 | 16 | 3 | 4 | 7 | 10 | 86 | 125 | 107 | 60 | 79 | 71 | 28 | 58 | 65 | 48 | 81 | 86 |
Unique Cell Count | 109 | 213 | 136 | 89 | 126 | 202 | 263 | 307 | 139 | 211 | 276 | 217 | 162 | 211 | 233 | 160 | 172 | 160 | 124 | 272 | 197 | 148 | 238 | 289 |
Labelled Cell Count | 136 | 268 | 149 | 100 | 137 | 250 | 331 | 370 | 203 | 292 | 409 | 309 | 198 | 244 | 265 | 185 | 197 | 191 | 124 | 272 | 197 | 148 | 238 | 289 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.5 | 4.3 | 5.0 | 4.8 | 5.8 | 4.4 | 4.6 | 4.9 | 4.2 | 3.9 | 3.8 | 4.1 | 5.2 | 5.4 | 5.4 | 7.1 | 7.2 | 7.1 | 4.7 | 4.7 | 5.2 |
Std Deviation (1e-4) | 0.6 | 0.7 | 0.7 | 1.4 | 2.1 | 1.1 | 1.1 | 1.3 | 1.6 | 1.3 | 1.0 | 1.1 | 0.8 | 1.3 | 1.4 | 1.5 | 1.5 | 1.4 | 1.1 | 1.1 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.05 | 0.2 | -0.19 | -0.13 | -0.04 | -0.25 | -0.35 | -0.41 | -0.3 | 0.05 | 0.09 | 0.11 | 0.5 | 0.53 | 0.49 | -0.11 | -0.11 | 0.05 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | 1.5 | 1.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.2561 | 3.8276 | 3.2932 | 3.1636 | 3.0628 | 3.6082 | 1.3824 | 1.9394 | 1.5376 | 1.1263 | 1.6306 | 1.4909 | 1.17 | 1.5184 | 0.9735 | 1.6213 | 1.1959 | 1.1351 |
Actin | 0.0483 | 0.0085 | 0.0204 | 0.0211 | 0.1088 | 0.0224 | 0.0229 | 0.0002 | 0.0185 | 0.0001 | 0.0186 | 0.0087 | 0.0678 | 0.0001 | 0.0193 | 0.0477 | 0.0893 | 0.0204 |
Bud | 0.0004 | 0.0002 | 0.0007 | 0.0011 | 0.0003 | 0.0004 | 0.0022 | 0 | 0.0006 | 0 | 0.0011 | 0.0006 | 0.001 | 0 | 0.0006 | 0.0004 | 0.0034 | 0.0001 |
Bud Neck | 0.0036 | 0.0013 | 0.0014 | 0.0007 | 0.0006 | 0.0089 | 0.0032 | 0.0001 | 0.0031 | 0.0001 | 0.0009 | 0.0021 | 0.0019 | 0.0002 | 0.0012 | 0.0007 | 0.0019 | 0.0015 |
Bud Periphery | 0.0005 | 0.0001 | 0.0012 | 0.0012 | 0.0007 | 0.0018 | 0.0069 | 0 | 0.0008 | 0 | 0.0013 | 0.0012 | 0.0019 | 0 | 0.0008 | 0.0006 | 0.008 | 0.0001 |
Bud Site | 0.0121 | 0.0048 | 0.0027 | 0.0207 | 0.0004 | 0.0006 | 0.006 | 0.0001 | 0.007 | 0 | 0.0021 | 0.0007 | 0.0035 | 0.0002 | 0.0055 | 0.001 | 0.0032 | 0.0001 |
Cell Periphery | 0.0004 | 0.0002 | 0.0002 | 0.0002 | 0 | 0.0005 | 0.0007 | 0 | 0.0002 | 0 | 0.0002 | 0.0002 | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0004 | 0 |
Cytoplasm | 0.0531 | 0.0413 | 0.0342 | 0.0469 | 0.0095 | 0.0348 | 0.0375 | 0.0412 | 0.027 | 0.0166 | 0.0198 | 0.0138 | 0.0366 | 0.0375 | 0.0228 | 0.0169 | 0.011 | 0.0137 |
Cytoplasmic Foci | 0.0179 | 0.0039 | 0.0065 | 0.0061 | 0.0159 | 0.003 | 0.0299 | 0.0014 | 0.0081 | 0.0002 | 0.0047 | 0.0027 | 0.0216 | 0.001 | 0.0009 | 0.0068 | 0.0199 | 0.0003 |
Eisosomes | 0.0005 | 0.0001 | 0.0008 | 0.0002 | 0.0005 | 0.0002 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0005 | 0 | 0.0001 | 0.0002 | 0.0006 | 0 |
Endoplasmic Reticulum | 0.0075 | 0.0008 | 0.001 | 0.0018 | 0.0021 | 0.0034 | 0.0085 | 0.0053 | 0.0032 | 0.001 | 0.0045 | 0.0029 | 0.0071 | 0.0027 | 0.0008 | 0.0004 | 0.0021 | 0.0004 |
Endosome | 0.0249 | 0.0007 | 0.0071 | 0.0043 | 0.0218 | 0.0152 | 0.0646 | 0.0075 | 0.0208 | 0.0006 | 0.0233 | 0.013 | 0.0293 | 0.0008 | 0.001 | 0.0052 | 0.0243 | 0.0008 |
Golgi | 0.0124 | 0.0004 | 0.0063 | 0.0036 | 0.0169 | 0.0109 | 0.0235 | 0.0002 | 0.0041 | 0 | 0.0187 | 0.0104 | 0.0121 | 0 | 0.0008 | 0.0094 | 0.0333 | 0.002 |
Lipid Particles | 0.0274 | 0.0002 | 0.0025 | 0.0006 | 0.0835 | 0.0047 | 0.0141 | 0.0002 | 0.0022 | 0 | 0.0104 | 0.0027 | 0.0119 | 0.0004 | 0.0006 | 0.0098 | 0.0144 | 0.0002 |
Mitochondria | 0.0063 | 0.0004 | 0.0068 | 0.0579 | 0.0042 | 0.0389 | 0.0163 | 0.0002 | 0.0178 | 0.0002 | 0.0317 | 0.0181 | 0.0145 | 0.0002 | 0.0012 | 0.0046 | 0.0435 | 0.0003 |
None | 0.058 | 0.0463 | 0.066 | 0.041 | 0.1053 | 0.0279 | 0.0221 | 0.005 | 0.0171 | 0.008 | 0.0008 | 0.0028 | 0.0245 | 0.0179 | 0.0235 | 0.0105 | 0.0123 | 0.0051 |
Nuclear Periphery | 0.0181 | 0.0085 | 0.009 | 0.0228 | 0.0384 | 0.008 | 0.0294 | 0.0163 | 0.0117 | 0.0092 | 0.0496 | 0.0165 | 0.0226 | 0.012 | 0.006 | 0.0043 | 0.0075 | 0.004 |
Nucleolus | 0.0057 | 0.0056 | 0.0033 | 0.0022 | 0.0021 | 0.0054 | 0.0035 | 0.0013 | 0.0019 | 0.0034 | 0.0047 | 0.0028 | 0.0025 | 0.0012 | 0.0025 | 0.0015 | 0.0039 | 0.0026 |
Nucleus | 0.6496 | 0.8382 | 0.8029 | 0.7432 | 0.5493 | 0.7955 | 0.6724 | 0.9151 | 0.8452 | 0.9588 | 0.7773 | 0.8924 | 0.7037 | 0.9222 | 0.9058 | 0.8639 | 0.6569 | 0.9448 |
Peroxisomes | 0.0103 | 0.0051 | 0.0047 | 0.0025 | 0.0255 | 0.0048 | 0.0069 | 0 | 0.002 | 0 | 0.0038 | 0.0019 | 0.0175 | 0 | 0.0009 | 0.0077 | 0.0068 | 0.0002 |
Punctate Nuclear | 0.04 | 0.0322 | 0.0207 | 0.0206 | 0.0112 | 0.0084 | 0.0098 | 0.0032 | 0.0041 | 0.0015 | 0.0196 | 0.0039 | 0.0115 | 0.0027 | 0.0049 | 0.008 | 0.054 | 0.0027 |
Vacuole | 0.002 | 0.001 | 0.0011 | 0.0008 | 0.0005 | 0.0028 | 0.0145 | 0.0019 | 0.0034 | 0.0002 | 0.003 | 0.0015 | 0.0054 | 0.0006 | 0.0007 | 0.0002 | 0.002 | 0.0006 |
Vacuole Periphery | 0.0008 | 0.0001 | 0.0007 | 0.0004 | 0.0022 | 0.0014 | 0.0049 | 0.0004 | 0.0011 | 0.0001 | 0.0037 | 0.001 | 0.0021 | 0.0001 | 0.0001 | 0.0002 | 0.0011 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 27.5146 | 25.6475 | 22.4634 | 29.8112 | 22.258 | 20.6833 | 24.0191 | 28.836 | 25.5362 | 32.2319 |
Translational Efficiency | 1.4721 | 1.0165 | 1.3621 | 1.0538 | 1.2983 | 1.665 | 1.2238 | 0.8817 | 1.1859 | 1.0219 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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