Standard name
Human Ortholog
Description GID Complex regulatory subunit; binds GID Complex in response to glucose through interactions with complex member Vid28p; regulates fructose-1,6-bisphosphatase (FBPase) targeting to the vacuole; promotes proteasome-dependent catabolite degradation of FBPase; peripheral membrane protein located at Vid (vacuole import and degradation) vesicles
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.95 0.93 0.98 0.93 0.89 0.81 0.49 0.56 – 0.58 0.54 0.59 0.94 0.9 0.89 0.95 0.9 0.92 0.86 0.78 0.91 0.82 0.75 0.62
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0.07 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Mitochondria 0 0 0 0 0 0.08 0.12 0.11 – 0.11 0.28 0.21 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0.07 0.06 0.08 – 0.09 0.06 0.07 0 0 0 0 0 0 0.08 0.13 0 0.08 0.1 0.08
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0.05 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.09 0 0 0.14 0.11 0.1 0.38 0.31 – 0.27 0.2 0.18 0.1 0.13 0.17 0.11 0.23 0.11 0 0.05 0 0.05 0.07 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 2 1 – 2 1 3 0 0 0 0 0 0 1 0 0 0 0 2
Bud 0 0 0 0 1 5 5 6 – 2 9 13 0 0 0 0 0 0 3 0 1 1 3 5
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 3 0 2 3 3 0 4 – 7 14 22 0 0 1 0 1 0 0 0 0 0 0 0
Cytoplasm 309 139 377 320 462 533 393 422 – 424 356 545 248 279 304 59 78 60 282 131 340 148 267 210
Endoplasmic Reticulum 3 2 0 2 7 5 11 9 – 22 6 19 3 6 1 3 6 1 2 1 2 2 7 11
Endosome 0 0 0 0 0 3 10 4 – 3 3 1 0 0 1 0 0 0 0 0 2 0 3 18
Golgi 0 2 1 0 2 0 0 0 – 0 0 0 2 0 0 0 0 2 1 0 0 0 1 15
Mitochondria 0 0 0 1 8 50 94 83 – 80 183 190 0 1 0 0 0 0 0 0 0 0 0 3
Nucleus 2 1 2 3 7 43 48 60 – 65 41 64 1 2 4 0 0 1 26 22 15 15 34 27
Nuclear Periphery 0 0 0 0 0 0 2 0 – 3 0 2 0 0 0 0 0 0 1 0 1 0 3 1
Nucleolus 0 8 0 0 0 0 8 6 – 2 0 2 1 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 0 0 0 4
Vac/Vac Membrane 30 4 12 49 58 64 307 231 – 199 129 161 26 41 57 7 20 7 3 8 3 9 26 34
Unique Cell Count 326 150 385 344 517 655 801 753 733 656 917 265 310 342 62 87 65 327 168 374 181 355 342
Labelled Cell Count 344 159 392 377 548 706 880 826 809 742 1022 281 329 368 69 106 71 327 168 374 181 355 342


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 19.5 23.2 13.6 15.2 12.3 9.0 7.9 8.1 – 8.4 8.6 9.2 21.7 19.8 19.3 17.6 20.5 19.9 20.3 20.2 20.1
Std Deviation (1e-4) 3.9 4.2 2.7 3.3 2.5 1.8 1.6 1.8 – 1.9 2.0 2.4 4.7 4.1 3.8 4.3 4.8 5.6 4.1 4.7 4.8
Intensity Change (Log2) – – – 0.16 -0.15 -0.59 -0.79 -0.75 – -0.71 -0.66 -0.57 0.67 0.54 0.5 0.37 0.59 0.55 0.57 0.57 0.56


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 2.9 3.1 0 0 0 0 0 0
Cytoplasm -3.2 -5.0 -7.8 -16.5 -14.6 – -14.1 -14.9 -13.9 -2.8 -4.5 -5.0 -1.3 -3.7 -2.5
Endoplasmic Reticulum 0 0 0 0 0 – 3.4 0 2.8 0 0 0 0 0 0
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 5.6 7.0 6.8 – 6.7 11.4 9.7 0 0 0 0 0 0
Nucleus 0 0 4.6 4.4 5.2 – 5.6 4.5 4.8 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 5.4 4.5 4.0 12.8 10.7 – 9.8 7.5 7.0 3.6 5.0 6.2 0 6.7 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 13.3662 20.2504 17.3002 16.8234 16.8923 18.1051 20.925 26.0164 24.0125 24.2278 21.4661 23.3228 17.1766 22.617 20.6613 15.8321 20.0162 19.4779
Actin 0.0053 0.0015 0.002 0.0045 0.0041 0.002 0.0128 0.0017 0.0014 0.0038 0.0014 0.0047 0.0005 0.0003 0.0005 0.0013 0.0013 0.0015
Bud 0.0014 0.002 0.0009 0.0003 0.0006 0.0005 0.0016 0.0021 0.0022 0.0103 0.0009 0.0023 0.0001 0.0002 0.0002 0.0004 0.0001 0.0005
Bud Neck 0.0025 0.0004 0.0009 0.0032 0.001 0.0015 0.0008 0.0003 0.0006 0.0009 0.0008 0.0017 0.0004 0.0003 0.0004 0.0008 0.0007 0.0011
Bud Periphery 0.0011 0.0004 0.0008 0.0005 0.0003 0.0003 0.0007 0.0008 0.0007 0.0048 0.0003 0.0018 0 0 0 0.0002 0 0.0003
Bud Site 0.0052 0.0026 0.0029 0.0116 0.0003 0.0002 0.0033 0.0025 0.0029 0.0004 0.0005 0.0002 0.0001 0.0004 0.0002 0.0005 0 0.0001
Cell Periphery 0.0003 0 0.0001 0.0003 0 0 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0 0 0 0 0 0
Cytoplasm 0.6502 0.8294 0.7273 0.6395 0.7303 0.7267 0.7898 0.8868 0.8335 0.8861 0.8643 0.7979 0.8386 0.8885 0.8192 0.7494 0.8523 0.7569
Cytoplasmic Foci 0.0455 0.0056 0.0106 0.0231 0.0146 0.0097 0.0075 0.0009 0.002 0.0009 0.0027 0.0031 0.001 0.001 0.0008 0.0074 0.0016 0.0009
Eisosomes 0 0 0 0.0002 0 0 0.0001 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.008 0.001 0.0022 0.0011 0.0053 0.0023 0.004 0.0008 0.001 0.0029 0.0023 0.0028 0.0022 0.0005 0.0011 0.0025 0.0011 0.0018
Endosome 0.031 0.0025 0.0216 0.0187 0.0198 0.0066 0.0064 0.0002 0.0006 0.0009 0.0008 0.0014 0.0005 0.0001 0.0004 0.0016 0.0004 0.0005
Golgi 0.0063 0.0012 0.0051 0.0058 0.0211 0.0022 0.0014 0 0.0001 0.0001 0.0001 0.0002 0 0 0 0.0002 0 0
Lipid Particles 0.0152 0.0001 0.0007 0.0068 0.0052 0.0012 0.0058 0 0 0 0.0001 0.0001 0 0 0 0.0003 0 0
Mitochondria 0.006 0.0005 0.0049 0.0037 0.0032 0.0005 0.0062 0.0002 0.0008 0.0004 0.0002 0.0005 0.0002 0.0001 0.0001 0.0002 0.0001 0.0001
None 0.0051 0.0009 0.0019 0.002 0.0014 0.0008 0.0044 0.0011 0.002 0.0018 0.0014 0.0025 0.001 0.0006 0.0005 0.0021 0.0006 0.0006
Nuclear Periphery 0.0286 0.0084 0.0135 0.0106 0.0184 0.0142 0.0133 0.0063 0.0088 0.0097 0.0125 0.0122 0.0135 0.0067 0.0112 0.0139 0.0098 0.0142
Nucleolus 0.0025 0.0001 0.0006 0.0006 0.0001 0.0001 0.0006 0.0001 0.0003 0.0001 0.0002 0.0003 0 0 0.0001 0.0002 0 0.0001
Nucleus 0.1454 0.1402 0.194 0.2596 0.1609 0.226 0.1242 0.0944 0.1382 0.0736 0.0983 0.1625 0.1398 0.0994 0.1631 0.2005 0.129 0.2185
Peroxisomes 0.0042 0.0004 0.0025 0.0033 0.0011 0.001 0.0072 0.0001 0.0002 0.0001 0.0001 0.0002 0 0 0 0.0003 0.0001 0
Punctate Nuclear 0.0192 0.0019 0.0024 0.0018 0.0098 0.0028 0.0064 0.0012 0.0035 0.0022 0.0121 0.0039 0.0012 0.0013 0.0017 0.0172 0.0023 0.0019
Vacuole 0.0135 0.0006 0.004 0.0021 0.0017 0.0012 0.0028 0.0004 0.001 0.0008 0.0009 0.0014 0.0006 0.0002 0.0004 0.0008 0.0004 0.0007
Vacuole Periphery 0.0034 0.0001 0.0011 0.0006 0.0008 0.0003 0.0006 0.0001 0.0003 0.0002 0.0002 0.0005 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 25.3307 28.9844 20.8356 23.3491 21.0191 28.3547 41.6696 37.3959 38.3954 35.6069
Translational Efficiency 1.0218 0.8307 0.9068 0.6325 0.6685 1.0712 0.7833 0.8928 0.8234 0.8147

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description GID Complex regulatory subunit; binds GID Complex in response to glucose through interactions with complex member Vid28p; regulates fructose-1,6-bisphosphatase (FBPase) targeting to the vacuole; promotes proteasome-dependent catabolite degradation of FBPase; peripheral membrane protein located at Vid (vacuole import and degradation) vesicles
Localization
Cell Percentages cytoplasm (85%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Vid24

Vid24


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vid24-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available