Standard name
Human Ortholog
Description Plasma membrane regulator of polyamine and carnitine transport; has similarity to transporters but lacks transport activity; may act as a sensor that transduces environmental signals; has a positive or negative regulatory effect on transcription of many transporter genes
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.07 0.05 0 0.05 0 0.14 0.16 0.15 0.16 0.19 0.14 0 0.11 0.2 0.2 – 0.21 0.08 0.11 0.18 0.09 0.08 0.09
Bud 0 0.06 0 0 0 0.05 0.07 0.08 0.08 0.09 0.08 0.06 0 0 0 0.08 – 0.05 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0.06 0.07 0.05 0.05 0.05 0.08
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – – – – – –
Cell Periphery 0 0.14 0.13 0.09 0.13 0.13 0.23 0.2 0.23 0.21 0.21 0.16 0.12 0.15 0.22 0.25 – 0.16 0.09 0.18 0.21 0.21 0.25 0.24
Cytoplasm 0.93 0.91 0.89 0.88 0.74 0.72 0.57 0.55 0.57 0.53 0.5 0.55 0.89 0.74 0.58 0.51 – 0.63 0.44 0.36 0.16 0.32 0.28 0.2
Endoplasmic Reticulum 0 0 0 0 0.17 0.12 0.13 0.08 0.05 0.05 0.09 0.08 0 0.12 0.13 0.06 – 0 0.13 0.12 0.17 0.1 0.15 0.17
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.11 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.07 0 0.15 0.18 0.18 0.27 0.28 0.31 0.26 0 0 0 0 – 0 0.07 0 0.12 0.12 0.06 0.08
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 – 0.05 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 – 0 0.06 0.05 0 0 0.05 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 11 16 14 5 14 9 56 73 47 64 56 47 48 131 205 13 – 4 22 26 52 39 58 61
Bud 6 15 9 6 6 11 28 34 26 34 25 21 11 33 37 5 – 1 2 4 1 11 20 28
Bud Neck 7 0 1 0 1 0 0 0 0 0 0 0 27 15 6 1 – 0 17 17 15 23 37 54
Bud Site 0 0 0 0 1 1 0 0 2 1 0 1 0 2 1 0 – 0 – – – – – –
Cell Periphery 12 34 37 25 36 32 92 88 73 83 61 52 137 175 231 16 – 3 25 46 61 91 173 166
Cytoplasm 247 219 247 237 209 173 233 245 178 211 148 179 1016 865 612 33 – 12 125 90 48 138 197 139
Endoplasmic Reticulum 6 1 7 7 47 29 52 35 15 21 26 26 48 138 136 4 – 0 38 30 50 45 107 115
Endosome 1 0 1 2 7 1 5 11 2 4 4 3 1 1 2 1 – 2 6 4 7 4 4 3
Golgi 3 1 0 0 0 0 0 0 0 1 0 0 0 0 1 0 – 0 1 3 0 1 0 3
Mitochondria 6 2 3 20 12 35 74 80 85 114 92 84 0 0 1 1 – 0 20 8 34 53 43 52
Nucleus 0 0 0 0 1 0 3 1 1 2 0 0 2 4 4 1 – 0 0 0 0 0 0 0
Nuclear Periphery 0 1 0 1 1 0 1 4 6 1 0 1 0 0 0 0 – 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 1 1 0 1 0 0 0 0 0 – 0 0 0 0 0 0 0
Peroxisomes 0 0 4 2 8 3 17 17 5 7 3 3 0 1 1 0 – 0 1 0 0 0 0 1
SpindlePole 1 1 4 2 3 1 1 2 0 2 2 2 5 11 21 5 – 1 1 2 0 2 1 3
Vac/Vac Membrane 0 1 1 2 6 1 2 12 6 11 4 12 4 13 38 3 – 0 18 13 11 16 35 42
Unique Cell Count 267 241 278 268 284 241 407 446 314 400 297 327 1144 1176 1047 65 19 287 253 293 440 698 689
Labelled Cell Count 300 291 328 309 352 296 564 603 447 556 422 431 1299 1389 1296 83 23 287 253 293 440 698 689


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.6 5.4 4.7 5.2 6.1 5.8 6.1 7.0 6.7 6.6 7.1 7.2 6.3 7.3 8.2 8.4 – 11.6 5.8 6.3 6.6
Std Deviation (1e-4) 0.9 1.3 1.2 2.1 2.3 2.0 1.8 2.5 2.3 2.4 2.7 2.9 1.0 1.2 1.6 1.7 – 2.0 1.6 1.7 1.9
Intensity Change (Log2) – – – 0.14 0.38 0.31 0.37 0.58 0.52 0.5 0.6 0.62 0.43 0.63 0.8 0.84 – 1.31 0.31 0.42 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -2.0 -0.1 -0.7 3.7 4.6 4.0 4.4 5.1 3.8 -0.6 3.1 5.8 4.0 – 0
Bud -0.7 -0.8 0.8 2.1 2.4 2.6 2.8 2.6 1.8 0 -0.4 0.2 0 – 0
Bud Neck 0 0 0 0 0 0 0 0 0 2.2 0 0 0 – 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Cell Periphery -1.5 -0.2 0 3.1 2.2 3.1 2.5 2.3 0.9 -0.6 0.7 3.2 2.3 – 0
Cytoplasm -0.2 -4.6 -4.9 -8.9 -9.5 -8.7 -9.9 -10.1 -9.2 0 -5.4 -9.4 -7.1 – -3.2
Endoplasmic Reticulum 0.1 5.6 4.3 4.7 3.0 1.5 1.8 3.2 2.9 1.3 4.6 5.0 0 – 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Mitochondria 3.7 2.3 5.9 7.0 6.9 8.9 9.3 9.6 8.6 0 0 0 0 – 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Peroxisomes 0 1.1 0 2.0 1.9 0 0 0 0 0 0 0 0 – 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0.6 0 – 0
Vacuole 0 0 0 0 0 0 0 0 2.8 0 0 2.9 0 – 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.6575 5.0297 4.6472 4.546 4.414 4.5908 4.1977 5.7634 4.9089 5.1492 4.6255 5.3688 5.9623 7.5278 6.7301 6.8138 6.5609 5.9809
Actin 0.0594 0.0371 0.0496 0.0524 0.0843 0.0377 0.0505 0.0161 0.0273 0.0051 0.0102 0.0261 0.0693 0.0063 0.0347 0.0084 0.0072 0.0352
Bud 0.0042 0.0008 0.0012 0.001 0.003 0.001 0.0013 0.0003 0.0004 0.0007 0.0029 0.0006 0.0011 0.0004 0.0004 0.0007 0.0004 0.0007
Bud Neck 0.0072 0.0084 0.0154 0.0171 0.0188 0.0351 0.0067 0.0143 0.0449 0.0414 0.0375 0.0494 0.0076 0.007 0.0204 0.0391 0.0137 0.017
Bud Periphery 0.0127 0.0031 0.0133 0.0087 0.0182 0.0099 0.0076 0.0021 0.005 0.0082 0.0125 0.0078 0.0101 0.0033 0.0039 0.0094 0.0048 0.0116
Bud Site 0.0174 0.0656 0.0398 0.0059 0.0046 0.0052 0.0079 0.0581 0.0405 0.0087 0.1436 0.0029 0.0251 0.0656 0.022 0.0148 0.0044 0.0031
Cell Periphery 0.2012 0.236 0.25 0.262 0.2581 0.2236 0.3982 0.4319 0.3811 0.4379 0.1658 0.3482 0.301 0.4581 0.4382 0.5251 0.4873 0.4306
Cytoplasm 0.2564 0.4788 0.4388 0.3595 0.3943 0.4742 0.2657 0.293 0.3808 0.3688 0.5989 0.405 0.2971 0.3631 0.3392 0.346 0.3139 0.3706
Cytoplasmic Foci 0.0324 0.007 0.0135 0.0135 0.0148 0.0084 0.0056 0.0024 0.0033 0.0056 0.0024 0.0056 0.0048 0.0016 0.0033 0.0016 0.0019 0.0028
Eisosomes 0.0242 0.023 0.0108 0.0047 0.0107 0.0037 0.0202 0.0054 0.0115 0.0025 0.0014 0.0075 0.0802 0.0042 0.0257 0.0025 0.0031 0.0206
Endoplasmic Reticulum 0.0757 0.0663 0.0724 0.1529 0.0461 0.0928 0.067 0.0417 0.0232 0.0694 0.0094 0.0862 0.0853 0.0522 0.0531 0.0378 0.1119 0.0605
Endosome 0.047 0.0025 0.0175 0.0117 0.0388 0.015 0.0021 0.0023 0.0002 0.0006 0.0002 0.0035 0.0085 0.0003 0.0009 0.0002 0.0006 0.0014
Golgi 0.022 0.0105 0.0095 0.0183 0.0193 0.0084 0.0023 0.0039 0.0011 0.0018 0.0005 0.0034 0.0226 0.0017 0.0059 0.0007 0.0024 0.0029
Lipid Particles 0.0294 0.0219 0.0205 0.0274 0.0205 0.0206 0.0116 0.0374 0.0061 0.0225 0.0053 0.0135 0.0262 0.0246 0.0124 0.0054 0.0359 0.0174
Mitochondria 0.0076 0.0002 0.0026 0.0025 0.0092 0.0088 0.0006 0.0047 0 0.0001 0 0.0002 0.0038 0 0.0001 0 0 0.0005
None 0.0497 0.028 0.0313 0.0505 0.0248 0.0411 0.0958 0.0741 0.0684 0.0177 0.0036 0.0302 0.043 0.0019 0.0211 0.0013 0.0006 0.0172
Nuclear Periphery 0.0237 0.0015 0.0029 0.002 0.0177 0.0023 0.0025 0.0029 0.0008 0.0007 0.0004 0.0013 0.0064 0.0012 0.0011 0.0011 0.0013 0.0012
Nucleolus 0.0048 0.0004 0.0002 0.0005 0.0004 0.0006 0.0004 0.0002 0.0002 0.0002 0.0004 0.0002 0.0004 0.0002 0.0001 0.0001 0.0001 0.0001
Nucleus 0.0662 0.0007 0.0006 0.0009 0.0023 0.0017 0.0017 0.0004 0.0004 0.0005 0.0006 0.0004 0.0009 0.0004 0.0004 0.0003 0.0002 0.0003
Peroxisomes 0.0213 0.0002 0.0031 0.0009 0.0037 0.0009 0.0008 0.0015 0.0002 0 0.0001 0.0014 0.0009 0 0.0001 0 0 0.0003
Punctate Nuclear 0.0105 0.0004 0.0011 0.0017 0.0032 0.0014 0.0002 0.0005 0.0002 0.0002 0.0006 0.0019 0.0012 0.0001 0.0001 0.0001 0.0001 0.0002
Vacuole 0.0234 0.0074 0.0054 0.0056 0.005 0.0074 0.0351 0.0067 0.0044 0.0073 0.0037 0.0048 0.0038 0.0076 0.0159 0.0054 0.0102 0.0058
Vacuole Periphery 0.0036 0.0001 0.0005 0.0003 0.0022 0.0005 0.0166 0.0003 0 0.0001 0 0.0001 0.0007 0 0.0011 0.0001 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.8709 18.1744 17.5729 22.3059 9.186 51.1066 37.5876 38.7113 44.5339 28.4633
Translational Efficiency 0.6038 0.6621 0.6139 0.9142 0.9085 0.6782 0.8014 0.8189 0.7804 0.6565

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Plasma membrane regulator of polyamine and carnitine transport; has similarity to transporters but lacks transport activity; may act as a sensor that transduces environmental signals; has a positive or negative regulatory effect on transcription of many transporter genes
Localization
Cell Percentages ER (9%), cytoplasm (7%), mixed (47%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Agp2

Agp2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Agp2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available