Standard name
Human Ortholog
Description Glycosyltransferase involved in protein glycosylation; transfers GDP-mannose to methyl-alpha-mannoside in vitro; member of the KRE2/MNT1 mannosyltransferase family of type II membrane proteins with a short cytoplasmic N-terminus, a membrane-spanning region and a highly conserved catalytic lumenal domain

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.07 0.09 0.07 0.26 0.11 0.15 0.15 0.22 0.35 0.48 0.47 0.44 0.67 0.91 0.2 0.19 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0
Endosome 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.11 0.17 0.24 0 0.08 0.11 0.08 0 0.1 0.12 0.09 0 0 0 0.1 0.06 0.05 0 0 0 0 0 0
Nucleus 0.32 0.15 0.15 0.14 0.14 0.27 0.28 0.26 0.14 0.08 0.1 0.08 0 0 0.47 0.43 0.41 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.67 0.8 0.74 0.8 0.82 0.72 0.72 0.74 0.64 0.62 0.6 0.6 0.4 0.15 0.45 0.41 0.59 0.83 0.88 0.92 0.9 0.88 0.83
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud 0 0 0 1 0 1 0 0 2 2 2 1 0 0 0 0 0 0 1 2 2 2 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 9 17 26 82 37 57 82 174 160 186 269 85 199 302 50 78 32 1 2 0 2 4 5
Endoplasmic Reticulum 0 1 0 0 0 2 1 3 0 2 0 1 3 2 11 25 17 0 0 0 0 2 2
Endosome 6 0 17 5 9 2 2 2 0 0 2 4 4 1 6 9 6 10 8 12 9 18 25
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 1 0 2 2
Mitochondria 14 33 89 14 27 40 43 25 46 45 53 0 0 0 26 26 13 2 4 6 4 7 11
Nucleus 39 28 58 45 46 102 155 210 66 32 55 15 13 2 116 175 112 4 1 2 2 2 1
Nuclear Periphery 3 2 1 2 3 3 5 12 1 3 2 0 0 0 2 3 1 2 0 2 1 3 5
Nucleolus 1 1 1 3 0 0 0 2 2 0 1 0 0 0 1 1 0 0 0 1 1 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 2 0 0 0 0 0 0 0 0 0 0 1 2 0 0 0 1 0 1 2
Vac/Vac Membrane 82 152 278 249 269 270 395 587 295 238 340 116 117 50 111 167 163 129 171 375 248 380 330
Unique Cell Count 123 189 375 312 329 374 548 794 459 385 570 194 295 333 248 406 274 157 195 408 276 432 398
Labelled Cell Count 154 235 470 403 391 477 683 1015 572 509 724 223 337 357 324 487 345 157 195 408 276 432 398


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 5.8 5.2 5.9 6.9 6.7 6.5 6.7 5.9 5.9 6.1 6.2 5.9 5.9 5.6 6.7 8.1 6.9 7.1 7.2
Std Deviation (1e-4) 1.0 1.1 1.2 1.4 1.6 2.1 1.9 1.7 1.7 1.7 1.7 0.9 1.0 1.2 1.5 2.0 2.3 2.1 1.6 1.7
Intensity Change (Log2) 0.17 0.4 0.35 0.32 0.35 0.18 0.18 0.21 0.24 0.17 0.17 0.11 0.36 0.63 0.4 0.43 0.46


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 6.9 2.0 3.6 3.7 6.3 9.6 12.7 13.1 10.5 16.5 22.3 4.9 5.0 2.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 4.9 4.9
Endosome -2.2 -1.3 -3.5 -4.4 -5.4 -4.6 -4.2 -4.5 -1.5 -2.3 -3.6 -1.4 -1.8 -1.6
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -7.0 -5.5 -4.7 -6.8 -11.1 -5.3 -4.4 -6.1 -7.4 -9.0 -9.5 -4.2 -6.8 -6.6
Nucleus -0.4 -0.6 3.9 4.5 4.2 -0.4 -3.1 -2.7 -2.6 -4.6 -7.1 8.5 8.4 7.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.8 2.4 -0.6 -0.7 -0.1 -3.1 -3.6 -4.6 -3.5 -9.0 -15.7 -7.4 -9.3 -3.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.0121 3.9476 3.1984 3.132 2.7937 3.4042 4.2604 6.2775 5.1629 5.0256 4.7281 5.6502 5.0015 7.4848 6.1941 6.305 5.3187 6.0723
Actin 0.0256 0.0003 0 0 0 0.0024 0.0099 0 0.0044 0 0.0195 0.0032 0.0037 0 0.002 0.0248 0 0
Bud 0.0006 0.0004 0.0003 0.0002 0.0002 0.0003 0.0002 0.0003 0.0007 0.0002 0.0003 0.0003 0.0006 0.0003 0.0005 0.0001 0.0002 0.0004
Bud Neck 0.0035 0.0009 0.0009 0.0005 0.0008 0.001 0.0007 0.0015 0.0039 0.0007 0.0011 0.0016 0.0025 0.0013 0.0029 0.0009 0.0011 0.0013
Bud Periphery 0.0017 0.0007 0.001 0.0004 0.0008 0.0011 0.0007 0.0007 0.0016 0.0005 0.0008 0.0011 0.0016 0.0008 0.0013 0.0003 0.0008 0.0014
Bud Site 0.0044 0.0018 0.0006 0.0003 0.0012 0.0005 0.0007 0.0013 0.0047 0.0007 0.0009 0.0008 0.0035 0.0009 0.0056 0.0006 0.0008 0.001
Cell Periphery 0.0035 0.0021 0.0028 0.0018 0.0033 0.0042 0.0025 0.0037 0.0029 0.001 0.0026 0.0039 0.003 0.0029 0.0031 0.0014 0.0018 0.0029
Cytoplasm 0.0152 0.0173 0.0063 0.0009 0.0007 0.0021 0.0049 0.0125 0.0056 0.0067 0.0019 0.0017 0.0028 0.0043 0.003 0.0013 0.0049 0.0013
Cytoplasmic Foci 0.0089 0.0057 0.0066 0.0024 0.0011 0.0049 0.0102 0.0041 0.0066 0.0045 0.0117 0.0028 0.0042 0.0046 0.0059 0.0071 0.006 0.0018
Eisosomes 0.0006 0 0 0 0 0 0.0001 0.0001 0.0001 0 0.0002 0 0.0001 0 0 0.0003 0 0
Endoplasmic Reticulum 0.0102 0.0018 0.0023 0.0005 0.0024 0.0018 0.0045 0.0033 0.0034 0.0016 0.0016 0.0018 0.0021 0.0032 0.0028 0.0005 0.0021 0.0015
Endosome 0.1456 0.0888 0.1986 0.1568 0.0886 0.1004 0.1735 0.1001 0.1441 0.1953 0.1653 0.1126 0.1456 0.1039 0.1431 0.1587 0.1847 0.1024
Golgi 0.0073 0.0019 0.004 0.0038 0.0019 0.0055 0.0077 0.004 0.009 0.0081 0.0084 0.004 0.0071 0.0047 0.0116 0.008 0.0042 0.0047
Lipid Particles 0.0261 0.0044 0.0066 0.0067 0.0138 0.021 0.0152 0.0076 0.0215 0.0077 0.0172 0.011 0.0249 0.01 0.0234 0.0059 0.0048 0.0172
Mitochondria 0.0362 0.013 0.0086 0.0019 0.0012 0.0161 0.0177 0.0041 0.0338 0.0326 0.0049 0.0056 0.0304 0.0026 0.0267 0.0014 0.0022 0.0091
None 0.0274 0.0026 0.0035 0.0001 0.0001 0.0006 0.0036 0.0065 0.0078 0.0067 0.001 0.0002 0.0014 0.0015 0.0005 0.0001 0.0004 0.0001
Nuclear Periphery 0.019 0.0064 0.0066 0.0011 0.0118 0.0168 0.006 0.0053 0.0124 0.0021 0.0027 0.0048 0.0056 0.0152 0.0106 0.0013 0.0053 0.0049
Nucleolus 0.0158 0.0038 0.0085 0.0035 0.0059 0.0031 0.0101 0.0039 0.0077 0.0024 0.003 0.0105 0.0129 0.0087 0.0066 0.0017 0.0023 0.0078
Nucleus 0.0338 0.0062 0.0042 0.0023 0.0044 0.005 0.0073 0.0046 0.0116 0.003 0.0023 0.0092 0.0082 0.0093 0.0146 0.0014 0.0099 0.0048
Peroxisomes 0.0027 0.0003 0.0006 0.0004 0.0005 0.0015 0.003 0.0006 0.0033 0.0006 0.0064 0.0012 0.002 0.0004 0.0018 0.0008 0.0002 0.0005
Punctate Nuclear 0.0097 0.0002 0.0001 0 0.0001 0.0027 0.0057 0.0001 0.0025 0.0001 0.0048 0.0002 0.0006 0.0002 0.0004 0.0001 0.0003 0.0001
Vacuole 0.4168 0.6571 0.5416 0.6535 0.704 0.6122 0.4864 0.6446 0.5002 0.5795 0.6074 0.6217 0.4982 0.621 0.5222 0.5885 0.6002 0.6053
Vacuole Periphery 0.1853 0.1842 0.1961 0.163 0.157 0.1968 0.2295 0.1911 0.2122 0.1458 0.1363 0.2017 0.2388 0.2042 0.2114 0.1951 0.1679 0.2314

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 56.1589 47.3743 55.6165 67.26 61.5317 45.8509 62.02 66.1063 74.933 67.6196
Translational Efficiency 1.0171 1.1003 0.8925 1.0151 0.81 1.5088 0.9639 0.9398 0.97 0.8688

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Glycosyltransferase involved in protein glycosylation; transfers GDP-mannose to methyl-alpha-mannoside in vitro; member of the KRE2/MNT1 mannosyltransferase family of type II membrane proteins with a short cytoplasmic N-terminus, a membrane-spanning region and a highly conserved catalytic lumenal domain
Localization
Cell Percentages vacuole (86%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Ktr4

Ktr4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ktr4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available