Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the small subunit
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0.09 0 0.05 0 0 0 0 0 0
Cytoplasm 0 0.07 0 0 0.08 0 0 – 0 0 0 0 0 0.06 0 0.18 0.13 0.19 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0 0.07 0 0 0 0 0 0
Endosome 0 0 0.19 0.08 0.07 0.07 0.09 – 0 0 0 0 0.13 0.18 0.31 0.15 0.18 0.12 0.07 0.05 0.06 0.06 0.05 0.05
Golgi 0 0 0.05 0.08 0 0 0 – 0 0 0 0 0.21 0.11 0.05 0 0.16 0.06 0.05 0.07 0 0.06 0.06 0.06
Mitochondria 0.9 0.9 0.6 0.8 0.81 0.78 0.79 – 0.94 0.88 0.92 0.92 0.63 0.51 0.49 0.08 0.13 0.1 0.76 0.75 0.78 0.7 0.68 0.71
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0.07 0 – 0.07 0.07 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.18 0.07 0 0 0 – 0 0 0 0 0.07 0.1 0.29 0.17 0.11 0.05 0 0 0.07 0.05 0.09 0.09
SpindlePole 0 0 0.12 0.06 0 0 0.07 – 0 0 0 0 0.06 0 0 0.18 0.16 0.19 0 0 0 0 0 0
Vac/Vac Membrane 0.18 0.15 0.1 0.07 0.14 0.12 0.11 – 0 0.05 0 0 0.11 0.17 0.16 0.19 0.22 0.24 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 2 6 5 5 0 1 – 1 1 2 1 4 6 0 0 4 4 5 3 6 13 14 8
Bud 3 7 11 7 9 6 10 – 15 10 22 17 3 4 4 8 4 4 2 0 0 2 3 1
Bud Neck 0 0 0 0 0 0 1 – 0 0 0 1 0 0 0 1 3 0 1 0 1 2 1 0
Bud Site 0 0 1 3 1 1 3 – 7 2 13 9 0 0 0 4 0 2 – – – – – –
Cell Periphery 5 10 18 8 2 2 7 – 6 5 10 8 11 17 16 16 8 11 1 1 0 0 1 0
Cytoplasm 5 18 10 11 22 13 8 – 5 4 8 9 6 24 20 34 26 42 0 2 0 3 2 3
Endoplasmic Reticulum 6 3 4 5 0 6 2 – 0 3 3 6 5 9 2 11 4 15 1 3 1 2 3 1
Endosome 12 6 74 19 19 29 42 – 2 9 27 9 34 72 150 28 36 27 30 14 30 27 22 22
Golgi 7 3 19 20 3 1 5 – 0 1 2 5 54 44 23 4 32 14 19 18 17 30 26 26
Mitochondria 351 243 232 198 225 308 364 – 461 401 711 818 165 204 240 15 25 22 312 203 377 349 292 318
Nucleus 0 1 4 2 6 7 17 – 7 3 5 8 0 1 2 7 4 12 0 1 0 3 1 1
Nuclear Periphery 14 3 2 3 1 26 17 – 34 33 39 58 3 3 1 3 3 3 6 2 2 5 3 1
Nucleolus 6 4 2 4 5 8 20 – 21 22 40 35 0 0 1 1 1 2 1 1 1 1 0 2
Peroxisomes 8 8 71 17 4 6 12 – 9 6 7 13 19 38 142 32 22 12 12 5 32 25 38 40
SpindlePole 7 2 48 15 8 16 32 – 15 15 17 19 17 17 22 33 32 41 2 2 2 5 2 4
Vac/Vac Membrane 69 41 37 18 40 49 49 – 18 23 33 33 28 67 80 35 42 53 13 9 3 19 8 10
Unique Cell Count 391 269 385 248 277 397 462 489 458 772 893 263 400 490 184 195 221 414 273 483 498 430 448
Labelled Cell Count 494 351 539 335 350 478 590 601 538 939 1049 349 506 703 232 246 264 414 273 483 498 430 448


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.7 5.2 6.3 6.3 5.5 5.9 5.8 – 4.9 5.6 5.6 5.5 6.2 6.2 6.1 5.3 6.7 6.2 7.9 7.7 7.6
Std Deviation (1e-4) 0.8 1.0 1.2 1.5 1.4 1.3 1.3 – 1.1 1.0 1.1 1.1 0.9 1.3 1.4 1.5 1.7 1.5 5.2 2.4 2.2
Intensity Change (Log2) – – – -0.01 -0.19 -0.1 -0.12 – -0.36 -0.18 -0.19 -0.19 -0.03 -0.04 -0.05 -0.25 0.07 -0.03 0.33 0.29 0.26


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 -0.1 0 0 0 0
Bud 0 0.3 -1.3 -0.6 – 0.2 -0.6 0 -1.1 -1.5 -1.9 -2.3 0.9 -0.6 -0.8
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.9 -2.9 -3.7 -2.7 – -3.1 -3.2 -3.5 -4.4 -0.3 -0.3 -1.1 1.9 -0.3 0.2
Cytoplasm 1.3 3.2 0.6 -0.9 – -1.8 -2.0 -2.0 -2.2 -0.3 2.3 1.2 6.6 5.1 6.9
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 1.3 0 0 0 3.9
Endosome -4.0 -4.5 -4.9 -4.3 – -9.8 -8.4 -8.9 -12.1 -2.1 -0.4 3.8 -1.2 -0.2 -2.2
Golgi 1.6 -2.7 -4.1 -3.4 – -5.0 -4.5 -5.6 -5.3 6.2 3.1 -0.2 -1.6 4.6 0.7
Mitochondria 5.2 5.8 5.2 5.9 – 12.3 9.1 13.1 13.4 0.6 -2.6 -3.3 -11.7 -10.9 -12.1
Nucleus 0 0 0.9 2.5 – 0 0 0 0 0 0 0 0 0 3.2
Nuclear Periphery 0 0 4.5 3.1 – 4.8 4.8 3.9 4.6 0 0 0 0 0 0
Nucleolus 0 0 0 3.5 – 3.5 3.7 4.0 3.3 0 0 0 0 0 0
Peroxisomes -4.1 -6.8 -7.9 -7.7 – -8.4 -8.6 -11.2 -11.2 -4.1 -3.6 3.6 -0.3 -2.2 -4.5
SpindlePole -2.6 -4.4 -4.3 -2.7 – -5.3 -5.1 -7.1 -7.6 -2.5 -4.2 -4.3 1.7 1.3 2.0
Vacuole -1.0 1.9 1.2 0.5 – -3.6 -2.6 -3.6 -4.3 0.4 2.9 2.9 3.2 4.0 4.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.0041 7.9802 8.2115 8.1048 7.2499 8.2142 9.9861 10.1422 10.218 9.4978 10.2221 10.2305 7.0761 6.4319 6.7015 6.1353 6.3537 6.697
Actin 0.0348 0.0054 0.0275 0.0002 0.0105 0.0071 0.0216 0.0087 0.0161 0.0013 0.0106 0.0051 0.0033 0.001 0.004 0.0003 0.002 0.0005
Bud 0.0004 0.0006 0.0007 0.0001 0.0003 0.0001 0.0002 0.0004 0.0002 0 0.0002 0.0001 0.0001 0.0001 0.0001 0 0.0001 0
Bud Neck 0.0101 0.0001 0.0005 0 0.0002 0.0003 0.0002 0.0001 0.0003 0 0.0002 0 0.0001 0.0001 0 0 0.0001 0
Bud Periphery 0.0009 0.0007 0.001 0.0006 0.0007 0.0004 0.0004 0.0006 0.0006 0.0001 0.0003 0.0003 0.0003 0.0002 0.0002 0.0001 0.0001 0.0001
Bud Site 0.0037 0.0031 0.0081 0 0.0006 0.0002 0.0006 0.0007 0.0015 0 0.0005 0.0001 0.0003 0.0002 0.0002 0 0.0001 0
Cell Periphery 0.0005 0.0001 0.0002 0.0001 0.0001 0.0005 0.0002 0.0002 0.0025 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0 0 0.0001
Cytoplasm 0.0036 0.0005 0.0004 0.0001 0.0001 0.0003 0.002 0.0002 0.0004 0 0.0004 0.0001 0 0.0001 0 0 0.0001 0
Cytoplasmic Foci 0.0157 0.0107 0.0067 0.0005 0.0071 0.0018 0.0049 0.0102 0.005 0.0011 0.005 0.001 0.0014 0.0092 0.0013 0.0002 0.0099 0.0009
Eisosomes 0.0011 0.0002 0.0006 0.0001 0.0006 0.0003 0.0005 0.0002 0.0011 0.0004 0.0002 0.0002 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0025 0.0001 0.0016 0.0001 0.0002 0.0013 0.0007 0.0004 0.0008 0 0.0007 0.0005 0.0001 0.0001 0 0 0.0001 0.0001
Endosome 0.0285 0.0166 0.0144 0.0049 0.0238 0.0053 0.0166 0.0234 0.0105 0.0048 0.0082 0.0036 0.0076 0.014 0.0066 0.0026 0.0166 0.0079
Golgi 0.0229 0.0267 0.0252 0.0124 0.0167 0.011 0.0243 0.0396 0.0273 0.0247 0.0083 0.0161 0.0176 0.0327 0.01 0.0122 0.0442 0.0141
Lipid Particles 0.0441 0.0067 0.0119 0.0006 0.018 0.0019 0.0181 0.0105 0.004 0.0003 0.0086 0.0024 0.0091 0.0045 0.004 0.0001 0.0017 0.0032
Mitochondria 0.7735 0.8892 0.8783 0.9779 0.9066 0.9458 0.8726 0.8748 0.9057 0.9647 0.948 0.9558 0.9481 0.9265 0.9623 0.9824 0.9163 0.9683
None 0.0118 0.0001 0.0006 0 0.0001 0.0003 0.0007 0.0001 0.0002 0 0.0006 0.0001 0 0 0 0 0 0
Nuclear Periphery 0.0047 0.0001 0.0006 0 0.0002 0.0077 0.0044 0.0003 0.0004 0 0.0008 0.0003 0.0001 0 0 0 0 0.0001
Nucleolus 0.0043 0.0008 0.0001 0 0.0002 0.0037 0.0004 0.0002 0.0001 0 0 0 0.0001 0.0001 0 0 0 0
Nucleus 0.0021 0.0002 0.0001 0 0.0001 0.0011 0.0012 0.0002 0.0001 0 0.0001 0 0 0 0 0 0 0
Peroxisomes 0.0219 0.0317 0.0185 0.0013 0.0108 0.0034 0.025 0.0194 0.0169 0.0015 0.0061 0.013 0.0089 0.009 0.0091 0.0005 0.0075 0.0029
Punctate Nuclear 0.0051 0.0001 0.0009 0 0.0001 0.0048 0.0018 0.0001 0.0001 0 0.0009 0 0 0 0 0 0 0
Vacuole 0.003 0.0032 0.0006 0.0003 0.0014 0.0006 0.0013 0.0055 0.0019 0.0001 0.0002 0.0002 0.0006 0.0008 0.0006 0.0002 0.0004 0.0004
Vacuole Periphery 0.0049 0.0031 0.0013 0.0008 0.0016 0.0021 0.0023 0.004 0.0042 0.0006 0.0003 0.0008 0.0017 0.0012 0.0011 0.0011 0.0007 0.0011

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.9416 10.6751 15.8427 21.4033 24.7725 14.322 21.3173 25.4972 25.2877 24.9506
Translational Efficiency 2.1362 2.3738 1.6983 1.3526 1.0258 2.4461 1.6796 1.4053 1.4917 1.6661

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the small subunit
Localization
Cell Percentages mitochondrion (98%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Mrps5

Mrps5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrps5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available