Standard name
Human Ortholog
Description Subunit of the Ssh1 translocon complex; Sec61p homolog involved in co-translational pathway of protein translocation; not essential

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.05 0.09 0.16 0.06 0.15 0.13 0.09 0.17 0.19 0.21 0 0 0 0.15 0.13 0 0 0 0.05 0.06 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.06 0.05 0 0.07 0.07 0.05 0.06 0.09 0.13 0.11 0.11 0.17 0.32 0.11 0.21 0 0 0 0 0.05 0.06
Cytoplasm 0 0.14 0 0 0.11 0.07 0.07 0.06 0.07 0.11 0.06 0.07 0.07 0.11 0.24 0.29 0.25 0 0 0 0 0 0
Endoplasmic Reticulum 0.92 0.84 0.88 0.71 0.61 0.78 0.77 0.8 0.71 0.59 0.63 0.88 0.89 0.82 0.07 0 0.09 0.93 0.87 0.91 0.76 0.86 0.82
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.15 0 0.28 0.42 0.31 0.13 0.12 0.08 0.06 0.07 0.1 0 0 0 0.31 0.36 0.51 0 0 0 0.05 0 0
Mitochondria 0 0 0 0.09 0 0.1 0.08 0.08 0.23 0.31 0.26 0 0 0 0.17 0.11 0.13 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 10 13 39 53 19 69 72 46 81 78 111 7 7 7 15 16 1 6 5 20 9 6 8
Bud 0 1 3 5 5 3 5 5 5 9 11 0 0 0 0 3 0 0 2 1 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 15 9 25 18 11 31 39 26 29 37 70 28 28 36 32 13 11 3 2 4 5 12 17
Cytoplasm 11 35 13 14 34 31 37 32 33 47 32 17 17 23 24 34 13 2 10 1 3 2 3
Endoplasmic Reticulum 359 209 373 240 190 350 420 404 329 240 343 222 220 174 7 4 5 374 239 388 113 239 245
Endosome 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 1 0 1
Golgi 59 1 121 140 95 58 67 41 30 28 56 8 8 3 31 43 27 4 1 6 6 8 9
Mitochondria 0 1 4 31 13 45 41 39 106 127 140 0 0 0 17 13 7 3 3 1 4 6 7
Nucleus 0 1 0 0 2 0 0 0 0 0 0 0 0 2 1 1 0 0 0 0 0 0 0
Nuclear Periphery 0 0 1 0 0 4 10 17 7 8 21 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 6 0 2 2 3 0 0 0 0 0 0 2 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 2 2 4 3 25 5 22 17 18 8 20 3 2 5 0 11 2 1 3 0 2 0 1
Unique Cell Count 390 248 425 336 311 451 544 504 466 409 541 251 247 212 101 119 53 401 275 429 150 280 301
Labelled Cell Count 456 272 583 504 401 596 715 629 641 582 804 285 282 250 128 140 66 401 275 429 150 280 301


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 47.1 39.5 35.8 35.0 27.9 26.5 25.2 23.9 20.2 19.6 21.0 42.8 42.8 41.2 25.0 25.5 35.7 40.1 46.1 50.4
Std Deviation (1e-4) 8.7 10.3 7.2 7.2 10.7 5.4 5.0 4.9 4.8 4.6 4.7 10.2 10.2 11.5 8.1 10.1 12.3 10.0 9.1 9.8
Intensity Change (Log2) -0.03 -0.36 -0.44 -0.51 -0.58 -0.82 -0.87 -0.77 0.26 0.26 0.2 -0.52 -0.49 -0.01 0.16 0.36 0.49


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 2.8 -1.5 2.8 2.0 0 3.6 4.1 4.8 -3.2 -3.1 -2.7 1.7 1.4 0
Bud 0 0 0 0 0 0 1.8 1.7 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.3 -1.5 0.6 0.8 -0.5 0.2 1.7 3.7 2.5 2.5 4.5 7.5 1.9 0
Cytoplasm 0.8 4.3 2.6 2.6 2.3 2.7 4.7 2.1 2.3 2.3 4.0 7.3 8.8 0
Endoplasmic Reticulum -5.7 -8.4 -4.0 -4.2 -3.1 -6.3 -9.5 -8.6 0.3 0.5 -1.9 -16.3 -17.6 -13.2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 3.8 0.6 -5.7 -6.3 -8.1 -8.8 -8.1 -7.2 -8.1 -8.0 -8.1 0.4 1.6 3.3
Mitochondria 5.4 2.9 5.8 4.8 4.9 9.9 11.9 10.8 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 3.5 0 0 3.8 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 4.9 0 3.0 2.5 2.8 1.2 2.7 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 54.1098 46.4739 49.9848 48.6 47.8981 49.4005 45.6975 52.4934 48.049 42.1503 39.0449 48.3323 54.5072 53.1437 53.105 47.5806 46.5621 50.4022
Actin 0.0007 0.0014 0.0011 0.0001 0.0005 0.0009 0.0088 0.0003 0.0012 0.0073 0.051 0.0077 0.0234 0.0002 0.0012 0.0066 0.0011 0.0028
Bud 0.0001 0.0002 0.0002 0 0 0.0001 0.0001 0.0001 0.0001 0.0003 0.0004 0.0001 0.0002 0 0.0001 0.0001 0.0001 0
Bud Neck 0.0004 0.0006 0.0039 0.0004 0.0022 0.0014 0.0002 0.0002 0.0002 0.0008 0.003 0.0018 0.0006 0.0003 0.0002 0.0006 0.0001 0.0004
Bud Periphery 0.0002 0.0006 0.0012 0 0.0003 0.0003 0.0004 0.0012 0.0003 0.0012 0.0007 0.0003 0.0005 0.0002 0.0004 0.0002 0.0001 0.0001
Bud Site 0.0003 0.0011 0.0002 0 0 0 0.0002 0 0.0002 0.0001 0.0033 0.0001 0.0007 0.0001 0.0002 0.0002 0.0001 0
Cell Periphery 0.0548 0.0868 0.0601 0.0492 0.0413 0.0264 0.0173 0.0349 0.0155 0.0191 0.0125 0.0061 0.024 0.0469 0.0259 0.0162 0.0031 0.004
Cytoplasm 0.0343 0.0601 0.0087 0.0069 0.0113 0.0129 0.0067 0.0042 0.0019 0.0016 0.0021 0.0018 0.0144 0.0071 0.0049 0.0007 0.0011 0.0011
Cytoplasmic Foci 0.0022 0.0011 0.0007 0.0001 0.0006 0.0005 0.0048 0.0001 0.003 0.002 0.018 0.0005 0.0101 0.0002 0.0011 0.0038 0.0011 0.001
Eisosomes 0.0025 0.0042 0.0038 0.0005 0.0012 0.0031 0.0033 0.0041 0.0012 0.007 0.0092 0.0008 0.0054 0.003 0.0017 0.0009 0.0046 0.0022
Endoplasmic Reticulum 0.8159 0.7525 0.8496 0.8604 0.8446 0.8901 0.8296 0.9112 0.9205 0.833 0.759 0.917 0.789 0.883 0.9062 0.8756 0.9063 0.9429
Endosome 0.0008 0.0021 0.0031 0.0005 0.005 0.0023 0.0055 0.001 0.0023 0.0038 0.0275 0.0036 0.0051 0.0014 0.0056 0.0049 0.0088 0.0028
Golgi 0.001 0.0047 0.0051 0.0011 0.0435 0.0077 0.0063 0.003 0.0113 0.0254 0.0284 0.023 0.0062 0.0195 0.0055 0.0235 0.0275 0.0181
Lipid Particles 0.0209 0.0419 0.0331 0.0327 0.0167 0.0209 0.0053 0.0078 0.0039 0.0095 0.0299 0.0037 0.0122 0.0119 0.005 0.0084 0.0087 0.0011
Mitochondria 0.0042 0.0006 0.0014 0 0.0004 0.0005 0.0182 0.0126 0.0139 0.0564 0.0053 0.008 0.0049 0.0013 0.0052 0.0127 0.0061 0.0011
None 0.0015 0.0004 0.0003 0.0001 0.0003 0.0002 0.0015 0.0006 0.0022 0.0039 0.0038 0.0001 0.006 0.0001 0.0004 0.0002 0.0015 0.0018
Nuclear Periphery 0.035 0.0187 0.0148 0.0375 0.0175 0.0245 0.0644 0.0096 0.0123 0.0155 0.0232 0.0192 0.0751 0.0139 0.0283 0.0219 0.0178 0.0144
Nucleolus 0.0039 0.0005 0.0002 0 0.0001 0.0001 0.0003 0.0001 0.0021 0.0008 0.0006 0 0.0015 0.0001 0.0001 0 0.0003 0.001
Nucleus 0.003 0.0016 0.0005 0.0011 0.0009 0.001 0.0032 0.0004 0.0008 0.0008 0.0008 0.0003 0.0028 0.0002 0.0003 0.0003 0.0007 0.0012
Peroxisomes 0.0001 0 0 0 0 0 0.0015 0 0.0006 0.0008 0.0059 0.0012 0.0011 0 0.0002 0.0187 0.0002 0.0001
Punctate Nuclear 0.0011 0.0003 0 0 0 0.0001 0.008 0 0.002 0.0005 0.0028 0 0.0034 0 0.0001 0.0001 0.0002 0.0019
Vacuole 0.0161 0.0197 0.0111 0.0083 0.0112 0.0059 0.0077 0.0048 0.0022 0.0028 0.0053 0.0023 0.0074 0.0085 0.0045 0.0027 0.0039 0.001
Vacuole Periphery 0.0013 0.0008 0.0009 0.0009 0.0022 0.0011 0.0065 0.0037 0.002 0.0074 0.0073 0.0025 0.006 0.0022 0.003 0.0018 0.0066 0.0012

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 185.0244 158.7041 152.5483 209.9723 175.7914 131.0176 170.9601 178.4493 171.5088 195.6213
Translational Efficiency 0.9551 1.0362 1.2762 0.7968 0.8548 1.3104 1.0045 0.814 0.8994 0.8584

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Subunit of the Ssh1 translocon complex; Sec61p homolog involved in co-translational pathway of protein translocation; not essential
Localization
Cell Percentages ER (97%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Ssh1

Ssh1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ssh1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available