Standard name | |
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Human Ortholog | |
Description | AP4A phosphorylase; bifunctional diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase and ADP sulfurylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; catalyzes phosphorolysis of dinucleoside oligophosphates, cleaving substrates' alpha/beta-anhydride bond and introducing Pi into the beta-position of the corresponding NDP formed; protein abundance increases under DNA replication stress; APA1 has a paralog, APA2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 5 | 3 | 4 | 10 | 11 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 3 | 8 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 1 | 2 | 5 | 7 | 8 | 14 | 5 | 9 | 8 | 11 | 0 | 3 | 0 | 15 | 1 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 393 | 237 | 268 | 275 | 354 | 330 | 329 | 340 | 239 | 286 | 208 | 179 | 258 | 341 | 454 | 357 | 195 | 299 | 378 | 223 | 250 | 238 | 347 | 385 |
Endoplasmic Reticulum | 1 | 3 | 1 | 2 | 8 | 12 | 21 | 45 | 11 | 24 | 9 | 9 | 0 | 1 | 0 | 9 | 5 | 9 | 3 | 1 | 1 | 1 | 2 | 3 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 5 | 3 | 1 | 2 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3 | 0 | 0 | 0 | 0 | 0 | 4 | 1 | 4 | 4 | 9 | 3 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 1 | 0 | 1 | 4 | 4 | 2 | 2 | 6 | 3 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 3 | 2 | 1 | 1 | 5 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 5 | 1 | 1 | 3 | 6 | 5 | 10 | 29 | 16 | 35 | 12 | 16 | 4 | 4 | 1 | 18 | 9 | 9 | 4 | 1 | 2 | 0 | 3 | 2 |
Unique Cell Count | 401 | 241 | 270 | 281 | 365 | 354 | 367 | 426 | 278 | 360 | 248 | 222 | 260 | 345 | 455 | 378 | 201 | 309 | 400 | 233 | 261 | 248 | 363 | 411 |
Labelled Cell Count | 405 | 241 | 273 | 282 | 374 | 360 | 377 | 439 | 286 | 373 | 260 | 234 | 262 | 349 | 456 | 405 | 212 | 323 | 400 | 233 | 261 | 248 | 363 | 411 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 38.6 | 49.9 | 35.0 | 33.6 | 31.6 | 28.6 | 26.9 | 26.8 | 24.0 | 24.2 | 22.1 | 23.4 | 39.1 | 41.8 | 42.7 | 48.8 | 47.3 | 44.3 | 43.5 | 45.3 | 51.3 |
Std Deviation (1e-4) | 8.4 | 9.9 | 7.2 | 6.6 | 7.0 | 6.2 | 6.1 | 6.7 | 6.7 | 7.0 | 6.1 | 6.7 | 9.2 | 10.0 | 9.5 | 16.1 | 16.3 | 15.1 | 9.1 | 10.6 | 13.8 |
Intensity Change (Log2) | – | – | – | -0.06 | -0.15 | -0.29 | -0.38 | -0.39 | -0.54 | -0.54 | -0.66 | -0.59 | 0.16 | 0.25 | 0.29 | 0.48 | 0.43 | 0.34 | 0.31 | 0.37 | 0.55 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
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Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 2.6 | 0 | 0 | 0 | 3.3 | 0 | 0 | 0 | 2.9 | 0 | 0 |
Cytoplasm | -1.4 | -2.0 | -3.7 | -4.9 | -7.5 | -5.9 | -7.6 | -6.4 | -7.1 | 0 | -0.5 | 1.1 | -3.3 | -1.9 | -2.1 |
Endoplasmic Reticulum | 0 | 0 | 2.6 | 3.7 | 5.3 | 2.9 | 4.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 4.1 | 3.6 | 5.0 | 3.2 | 4.1 | 0 | 0 | 0 | 3.3 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 22.4145 | 26.935 | 26.3434 | 24.4119 | 25.3525 | 26.73 | 28.9605 | 32.9176 | 31.3329 | 31.4705 | 29.1736 | 30.8119 | 29.0343 | 32.6417 | 31.7133 | 32.4848 | 29.0149 | 31.0628 |
Actin | 0.0039 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0029 | 0.0001 | 0.0009 | 0 | 0.0017 | 0.001 | 0.0041 | 0.0003 | 0.0007 | 0 | 0.0001 | 0.0005 |
Bud | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0005 | 0 | 0.0001 | 0 | 0.0012 | 0.0001 | 0.0005 | 0.0002 | 0.0004 | 0 | 0 | 0.0005 |
Bud Neck | 0.0013 | 0.0002 | 0.0002 | 0.0003 | 0.0003 | 0.0006 | 0.0006 | 0.0002 | 0.0003 | 0.0002 | 0.0013 | 0.0009 | 0.001 | 0.0002 | 0.0003 | 0.0002 | 0.0003 | 0.0008 |
Bud Periphery | 0.0003 | 0 | 0 | 0 | 0 | 0.0001 | 0.0004 | 0 | 0.0001 | 0 | 0.0018 | 0.0001 | 0.0004 | 0 | 0.0002 | 0 | 0 | 0.0002 |
Bud Site | 0.0006 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0005 | 0 | 0.0002 | 0 | 0.001 | 0 | 0.0011 | 0.0001 | 0.0001 | 0 | 0 | 0.0008 |
Cell Periphery | 0.0002 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0.0008 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Cytoplasm | 0.8382 | 0.9688 | 0.951 | 0.9091 | 0.9708 | 0.9195 | 0.9055 | 0.9826 | 0.9531 | 0.9649 | 0.8997 | 0.9282 | 0.9058 | 0.9753 | 0.9573 | 0.9718 | 0.9792 | 0.9371 |
Cytoplasmic Foci | 0.0086 | 0.0012 | 0.0003 | 0.001 | 0.0005 | 0.0007 | 0.0073 | 0.0003 | 0.0022 | 0.0001 | 0.0025 | 0.0007 | 0.0026 | 0.0004 | 0.0006 | 0.0002 | 0.0002 | 0.0016 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0026 | 0.0006 | 0.0008 | 0.0006 | 0.0009 | 0.0024 | 0.0041 | 0.0004 | 0.0011 | 0.0002 | 0.0051 | 0.002 | 0.0014 | 0.0029 | 0.0009 | 0.0003 | 0.0003 | 0.001 |
Endosome | 0.0116 | 0.0002 | 0.0001 | 0.0017 | 0.0002 | 0.0007 | 0.0042 | 0 | 0.0031 | 0 | 0.0045 | 0.0009 | 0.0023 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0009 |
Golgi | 0.0019 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0004 | 0 | 0.0005 | 0 | 0.0008 | 0.0005 | 0.0009 | 0 | 0 | 0 | 0 | 0.0003 |
Lipid Particles | 0.0062 | 0 | 0 | 0.0002 | 0 | 0.0002 | 0.0006 | 0 | 0.0009 | 0 | 0.0034 | 0.0016 | 0.0019 | 0 | 0 | 0 | 0 | 0.0012 |
Mitochondria | 0.0065 | 0.0003 | 0.0005 | 0.0009 | 0 | 0.0007 | 0.0025 | 0 | 0.0007 | 0 | 0.0174 | 0.004 | 0.0046 | 0.0001 | 0.0002 | 0 | 0 | 0.0005 |
None | 0.0026 | 0.0006 | 0.0006 | 0.001 | 0.0003 | 0.0009 | 0.0019 | 0.0002 | 0.0005 | 0.0001 | 0.0015 | 0.0006 | 0.0016 | 0.0003 | 0.0007 | 0.0002 | 0.0002 | 0.0006 |
Nuclear Periphery | 0.0122 | 0.0028 | 0.0043 | 0.0084 | 0.0037 | 0.0073 | 0.0083 | 0.0012 | 0.003 | 0.0014 | 0.0053 | 0.0054 | 0.0075 | 0.0026 | 0.0036 | 0.0029 | 0.0022 | 0.0061 |
Nucleolus | 0.0022 | 0.0017 | 0.0018 | 0.0034 | 0 | 0.0003 | 0.0003 | 0 | 0.0001 | 0 | 0.0014 | 0 | 0.0017 | 0 | 0 | 0 | 0 | 0.0005 |
Nucleus | 0.0853 | 0.0222 | 0.0393 | 0.0659 | 0.022 | 0.0637 | 0.054 | 0.0141 | 0.0288 | 0.0329 | 0.0405 | 0.0523 | 0.056 | 0.0166 | 0.0327 | 0.0236 | 0.0169 | 0.0442 |
Peroxisomes | 0.0011 | 0 | 0 | 0 | 0 | 0 | 0.0015 | 0 | 0.0003 | 0 | 0.0009 | 0.0003 | 0.001 | 0 | 0 | 0 | 0 | 0.0007 |
Punctate Nuclear | 0.0099 | 0.0004 | 0.0002 | 0.0012 | 0.0006 | 0.0007 | 0.0013 | 0.0001 | 0.0009 | 0.0001 | 0.0005 | 0.0004 | 0.0038 | 0.0003 | 0.0014 | 0.0002 | 0.0002 | 0.0015 |
Vacuole | 0.003 | 0.0006 | 0.0005 | 0.0035 | 0.0004 | 0.0011 | 0.0027 | 0.0005 | 0.0023 | 0.0001 | 0.0068 | 0.0009 | 0.001 | 0.0004 | 0.0004 | 0.0004 | 0.0003 | 0.0007 |
Vacuole Periphery | 0.0012 | 0.0001 | 0.0002 | 0.0023 | 0.0001 | 0.0003 | 0.0006 | 0.0001 | 0.0008 | 0 | 0.0016 | 0.0002 | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0002 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 138.7822 | 164.396 | 134.1121 | 146.2851 | 134.7678 | 110.736 | 170.8666 | 153.9968 | 142.4016 | 142.1624 |
Translational Efficiency | 2.5867 | 2.537 | 2.5175 | 1.9569 | 2.5689 | 3.3629 | 2.2777 | 2.4731 | 2.3795 | 2.3459 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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