ORF | |
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Human Ortholog | |
Description | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 |
Bud | 0 | 1 | 0 | 0 | 2 | 2 | 4 | 1 | 8 | 7 | 2 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 3 | 2 | 4 | 8 | 9 |
Bud Neck | 3 | 1 | 5 | 3 | 11 | 3 | 3 | 3 | 2 | 1 | 2 | 5 | 0 | 8 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 5 |
Bud Site | 0 | 3 | 1 | 4 | 9 | 8 | 8 | 1 | 22 | 14 | 9 | 28 | 0 | 0 | 0 | 1 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
Cytoplasm | 4 | 5 | 10 | 5 | 3 | 9 | 17 | 22 | 9 | 8 | 4 | 6 | 11 | 28 | 38 | 17 | 18 | 15 | 0 | 0 | 2 | 31 | 32 | 25 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 1 | 2 | 0 | 0 | 0 | 2 | 5 | 2 |
Endosome | 5 | 11 | 9 | 8 | 4 | 29 | 30 | 32 | 9 | 21 | 11 | 8 | 11 | 11 | 26 | 6 | 3 | 1 | 6 | 9 | 12 | 9 | 9 | 9 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 1 | 5 | 3 | 0 | 1 | 2 |
Mitochondria | 20 | 22 | 5 | 6 | 35 | 22 | 30 | 22 | 115 | 76 | 78 | 104 | 1 | 2 | 1 | 29 | 6 | 10 | 2 | 7 | 0 | 10 | 25 | 9 |
Nucleus | 121 | 107 | 48 | 22 | 22 | 38 | 42 | 55 | 58 | 68 | 54 | 85 | 23 | 21 | 25 | 69 | 26 | 29 | 76 | 69 | 40 | 19 | 13 | 11 |
Nuclear Periphery | 2 | 6 | 4 | 0 | 1 | 2 | 2 | 2 | 0 | 12 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 2 | 7 |
Nucleolus | 352 | 403 | 228 | 172 | 167 | 282 | 360 | 277 | 374 | 349 | 275 | 342 | 141 | 199 | 236 | 197 | 115 | 115 | 335 | 379 | 189 | 134 | 138 | 172 |
Peroxisomes | 0 | 3 | 2 | 0 | 0 | 4 | 3 | 2 | 5 | 4 | 1 | 1 | 1 | 1 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 21 | 56 | 76 | 71 | 57 | 145 | 197 | 162 | 179 | 200 | 94 | 135 | 48 | 93 | 104 | 17 | 10 | 15 | 20 | 36 | 37 | 30 | 46 | 40 |
Vac/Vac Membrane | 4 | 7 | 1 | 2 | 0 | 3 | 0 | 2 | 2 | 0 | 1 | 2 | 1 | 3 | 3 | 8 | 3 | 3 | 6 | 6 | 8 | 3 | 8 | 7 |
Unique Cell Count | 436 | 491 | 289 | 203 | 204 | 369 | 453 | 391 | 502 | 484 | 355 | 492 | 168 | 253 | 297 | 268 | 158 | 155 | 461 | 523 | 301 | 256 | 307 | 313 |
Labelled Cell Count | 532 | 625 | 389 | 293 | 311 | 547 | 696 | 581 | 783 | 760 | 535 | 728 | 239 | 367 | 439 | 348 | 184 | 192 | 461 | 523 | 301 | 256 | 307 | 313 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.2 | 9.6 | 9.9 | 8.6 | 6.1 | 6.8 | 6.7 | 6.8 | 5.6 | 6.0 | 5.7 | 5.7 | 9.8 | 9.5 | 9.2 | 10.7 | 13.6 | 14.3 | 9.6 | 10.2 | 11.0 |
Std Deviation (1e-4) | 2.7 | 2.3 | 2.6 | 2.0 | 1.6 | 1.6 | 1.7 | 1.9 | 1.9 | 1.6 | 1.4 | 1.6 | 2.8 | 3.1 | 3.0 | 3.5 | 4.3 | 6.3 | 2.4 | 2.5 | 2.9 |
Intensity Change (Log2) | – | – | – | -0.21 | -0.7 | -0.54 | -0.56 | -0.54 | -0.83 | -0.72 | -0.8 | -0.81 | -0.02 | -0.07 | -0.11 | 0.1 | 0.45 | 0.52 | -0.05 | 0.04 | 0.14 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 2.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.1 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 3.3 | 2.5 | 0 | 3.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.6 | -1.4 | -0.8 | 0.2 | 1.3 | -1.5 | -1.6 | -2.0 | -2.1 | 1.5 | 3.5 | 4.1 | 1.6 | 3.3 | 2.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0.5 | -0.8 | 2.6 | 2.1 | 2.7 | -1.2 | 0.9 | 0 | -1.4 | 1.7 | 0.8 | 2.9 | -0.6 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 6.2 | 2.7 | 3.1 | 2.6 | 8.0 | 6.1 | 7.6 | 7.6 | 0 | 0 | 0 | 4.5 | 0 | 2.6 |
Nucleus | -1.8 | -1.8 | -2.4 | -3.0 | -0.9 | -2.0 | -1.0 | -0.5 | 0.2 | -0.8 | -2.9 | -3.0 | 2.6 | 0 | 0.6 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1.6 | 0.8 | -0.8 | 0.2 | -2.4 | -1.4 | -2.1 | -0.4 | -2.9 | 1.3 | -0.1 | 0.2 | -1.5 | -1.5 | -1.1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 2.1 | 0.4 | 3.5 | 4.7 | 4.1 | 2.7 | 4.2 | 0.1 | 0.3 | 0.5 | 2.6 | 2.3 | -6.3 | -5.1 | -4.1 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 10.1621 | 11.9156 | 10.3506 | 9.693 | 10.3087 | 10.3349 | 10.3852 | 15.3347 | 13.0148 | 11.3454 | 13.018 | 13.0162 | 10.6863 | 11.0685 | 10.4345 | 9.3973 | 10.3193 | 10.4072 |
Actin | 0.0199 | 0 | 0.0074 | 0 | 0.0002 | 0.0021 | 0.0005 | 0 | 0.0162 | 0.0734 | 0.0002 | 0 | 0.0158 | 0.0003 | 0.0013 | 0.0085 | 0 | 0.001 |
Bud | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0004 | 0.0003 | 0.0008 | 0 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0 |
Bud Neck | 0.0057 | 0.0003 | 0.0004 | 0.0002 | 0.0014 | 0.0002 | 0.0004 | 0.0001 | 0.0006 | 0.0004 | 0.0012 | 0.0001 | 0.0008 | 0.0002 | 0.0002 | 0.0004 | 0.0002 | 0.0003 |
Bud Periphery | 0.0004 | 0 | 0.0003 | 0 | 0.0003 | 0 | 0.0003 | 0.0001 | 0.0013 | 0.0004 | 0.0017 | 0 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0 | 0 |
Bud Site | 0.0066 | 0.0002 | 0.001 | 0.0001 | 0.0002 | 0.0001 | 0.0009 | 0.0005 | 0.0019 | 0.0006 | 0.0019 | 0 | 0.0014 | 0.0012 | 0.0002 | 0.0008 | 0 | 0.0002 |
Cell Periphery | 0.0004 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0008 | 0.0001 | 0.001 | 0.0001 | 0.0004 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0006 | 0.0001 | 0 |
Cytoplasm | 0.001 | 0 | 0.0007 | 0 | 0 | 0 | 0.1292 | 0 | 0.0029 | 0.0002 | 0.0035 | 0 | 0.0048 | 0 | 0.0003 | 0.0016 | 0 | 0 |
Cytoplasmic Foci | 0.0059 | 0.0001 | 0.0074 | 0.0001 | 0.0013 | 0.0008 | 0.0319 | 0.0001 | 0.0236 | 0.0161 | 0.0053 | 0.0002 | 0.012 | 0.0025 | 0.0083 | 0.0175 | 0 | 0.0019 |
Eisosomes | 0.0004 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0004 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0003 | 0 | 0 |
Endoplasmic Reticulum | 0.0011 | 0 | 0.0009 | 0 | 0 | 0 | 0.0046 | 0 | 0.0064 | 0.0002 | 0.002 | 0 | 0.0019 | 0 | 0.0004 | 0.0082 | 0 | 0 |
Endosome | 0.0046 | 0 | 0.0067 | 0 | 0.0003 | 0.0008 | 0.0059 | 0 | 0.0185 | 0.012 | 0.1139 | 0 | 0.0157 | 0.0002 | 0.0034 | 0.0049 | 0 | 0.0004 |
Golgi | 0.0066 | 0 | 0.0021 | 0 | 0.0003 | 0.002 | 0.001 | 0 | 0.0148 | 0.0531 | 0.006 | 0 | 0.0045 | 0.0003 | 0.0009 | 0.0033 | 0 | 0.001 |
Lipid Particles | 0.0095 | 0.0003 | 0.0048 | 0.0002 | 0.0048 | 0.0023 | 0.0195 | 0.0002 | 0.0146 | 0.0128 | 0.0033 | 0.0013 | 0.0045 | 0.0018 | 0.0114 | 0.0199 | 0.0001 | 0.0027 |
Mitochondria | 0.0151 | 0 | 0.0037 | 0.0001 | 0.0037 | 0.0013 | 0.008 | 0.0011 | 0.017 | 0.0065 | 0.0036 | 0.0001 | 0.0012 | 0.0009 | 0.0038 | 0.0158 | 0 | 0.0004 |
None | 0.0017 | 0 | 0.0026 | 0 | 0.0001 | 0 | 0.0034 | 0 | 0.0041 | 0.0002 | 0.0004 | 0 | 0.0007 | 0 | 0.0001 | 0.0029 | 0 | 0 |
Nuclear Periphery | 0.007 | 0 | 0.0049 | 0 | 0.0013 | 0.0001 | 0.0054 | 0 | 0.0239 | 0.0001 | 0.0033 | 0 | 0.0061 | 0 | 0.001 | 0.0108 | 0 | 0.0001 |
Nucleolus | 0.8159 | 0.867 | 0.867 | 0.9189 | 0.8454 | 0.8952 | 0.6886 | 0.8253 | 0.748 | 0.7062 | 0.7358 | 0.9053 | 0.8296 | 0.84 | 0.8715 | 0.7238 | 0.8851 | 0.8919 |
Nucleus | 0.0872 | 0.1291 | 0.0833 | 0.0793 | 0.0891 | 0.0829 | 0.0544 | 0.1711 | 0.0727 | 0.0706 | 0.0755 | 0.0875 | 0.0837 | 0.148 | 0.0839 | 0.15 | 0.1095 | 0.0941 |
Peroxisomes | 0.0049 | 0 | 0.0026 | 0 | 0.0004 | 0.002 | 0.0016 | 0 | 0.02 | 0.0457 | 0.0005 | 0 | 0.0017 | 0.0019 | 0.0047 | 0.0114 | 0 | 0.001 |
Punctate Nuclear | 0.0048 | 0.0023 | 0.002 | 0.0005 | 0.0502 | 0.0099 | 0.0311 | 0.0008 | 0.0021 | 0.0008 | 0.0102 | 0.0052 | 0.013 | 0.0019 | 0.007 | 0.013 | 0.0046 | 0.0044 |
Vacuole | 0.0007 | 0.0004 | 0.0011 | 0.0003 | 0.0004 | 0.0001 | 0.0072 | 0.0002 | 0.0051 | 0.0002 | 0.0243 | 0.0001 | 0.0013 | 0.0003 | 0.0009 | 0.0022 | 0.0001 | 0.0002 |
Vacuole Periphery | 0.0005 | 0 | 0.0006 | 0.0001 | 0.0004 | 0 | 0.0051 | 0.0002 | 0.0043 | 0.0002 | 0.0062 | 0 | 0.0006 | 0.0001 | 0.0005 | 0.0038 | 0 | 0.0001 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 32.862 | 31.6363 | 25.9908 | 28.8423 | 27.0391 | 32.8438 | 29.4171 | 19.2762 | 25.3563 | 34.4046 |
Translational Efficiency | 3.4997 | 2.6678 | 2.9045 | 2.2232 | 3.3553 | 2.2284 | 1.7574 | 2.0858 | 1.6693 | 1.8923 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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