ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; contains ankyrin (Ank) repeats; YCR051W is not an essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 – 0 0 0 0 – 0 0.05 0 0 0.05 0.14 0.21 0.15 0.21 0.21 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 – 0 0 0.14 0 – 0.06 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.84 0.83 – 0.98 0.95 0.88 0.91 – 0.88 0.87 0.9 0.93 0.91 0.73 0.69 0.69 0.64 0.64 0.88 0.91 0.92 0.85 0.69 0.61
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.13 0 – 0 0 0.13 0.05 – 0.06 0.08 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0.06 0.1
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.14 – 0 0 0 0 – 0 0 0 0 0.05 0.15 0.12 0.16 0.21 0.22 0 0.06 0.05 0.07 0.17 0.17
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 0 3 0 0 1 0 0 0 0 0 0 1
Bud 0 1 – 0 0 0 0 – 1 1 1 1 0 1 0 0 1 0 3 0 0 16 18 17
Bud Neck 0 0 – 0 0 0 0 – 0 1 0 1 0 0 0 0 0 0 0 0 0 1 1 2
Bud Site 0 0 – 0 0 1 0 – 1 3 8 6 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 – 0 0 0 0 – 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 0 – 4 7 6 15 – 10 18 9 6 29 107 175 28 29 18 3 0 0 3 6 7
Endoplasmic Reticulum 0 0 – 0 0 0 0 – 0 0 0 0 1 0 0 5 1 3 0 0 0 2 1 4
Endosome 0 1 – 0 0 0 1 – 1 0 0 0 2 4 11 0 0 1 1 0 0 3 8 13
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 1 0 0 1 3 2 0 0 0 2 2 4
Mitochondria 2 0 – 0 0 25 4 – 20 12 22 16 0 0 1 2 1 1 2 0 0 0 3 6
Nucleus 127 29 – 172 267 152 348 – 305 332 338 302 538 569 586 127 87 56 172 51 42 563 558 508
Nuclear Periphery 2 0 – 0 0 1 1 – 1 2 1 0 0 0 0 0 0 0 0 0 0 0 2 4
Nucleolus 19 1 – 0 8 22 18 – 19 30 25 16 5 3 8 1 0 0 2 0 0 15 45 79
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 – 0 0 0 1 – 0 0 0 0 0 1 2 0 0 0 0 0 0 2 14 27
Vac/Vac Membrane 3 5 – 0 4 1 9 – 11 15 7 6 30 117 105 29 28 19 6 3 2 46 139 144
Unique Cell Count 151 35 176 281 173 383 345 380 374 324 593 777 851 184 136 87 195 56 46 662 811 828
Labelled Cell Count 159 37 176 286 208 397 369 414 411 354 606 807 892 193 151 100 195 56 46 662 811 828


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.3 9.2 – 10.9 10.4 6.2 8.2 – 7.3 7.8 7.3 7.3 12.8 11.8 11.2 20.4 21.7 21.9 10.9 10.5 10.6
Std Deviation (1e-4) 2.6 2.0 – 2.5 2.2 1.9 1.8 – 1.6 1.8 1.5 1.5 3.1 2.9 3.0 6.8 6.2 7.6 2.5 2.2 2.4
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.6219 5.6972 4.5555 4.0097 2.7851 4.6747 5.7108 8.1855 7.1466 6.616 4.6956 6.7933 7.3735 9.4685 8.3812 8.1994 7.2015 7.9902
Actin 0.0272 0 0 0.0415 0.0409 0.0017 0.0185 0 0.0049 0.0001 0.0046 0.0042 0 0 0 0 0 0
Bud 0.0002 0 0.0002 0.0002 0.0007 0 0.0004 0 0.0001 0.0001 0.0007 0.0001 0 0.0002 0 0 0 0
Bud Neck 0.003 0 0.0002 0.0004 0.0009 0.0008 0.0045 0.0001 0.0001 0.0001 0.0021 0.0007 0 0.0002 0 0 0.0001 0.003
Bud Periphery 0.0003 0 0.0005 0.0002 0.0013 0.0001 0.0008 0 0.0001 0.0002 0.0017 0.0002 0 0.0001 0 0 0 0.0001
Bud Site 0.0029 0 0.0002 0.0013 0.0015 0.0001 0.0048 0 0.0003 0.0003 0.0018 0.0002 0 0.0011 0 0 0 0.0002
Cell Periphery 0.0001 0 0.0002 0 0.0002 0 0.0003 0 0 0.0002 0.0005 0.0001 0 0 0 0 0 0
Cytoplasm 0.0028 0.0002 0.0008 0.0007 0.0022 0.0002 0.0026 0.0002 0.0012 0.0005 0.0007 0.0007 0.0005 0.0017 0.001 0.0002 0.0003 0.0012
Cytoplasmic Foci 0.0062 0 0 0.0039 0.014 0.0012 0.0173 0 0.0026 0.0042 0.006 0.0025 0 0 0 0 0 0
Eisosomes 0.0001 0 0 0.0001 0.0003 0 0.0003 0 0.0001 0.0001 0.0002 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.001 0 0.0003 0.0005 0.0016 0 0.0019 0 0.0001 0.0003 0.0006 0.0012 0 0 0 0 0 0.0001
Endosome 0.0088 0 0.0003 0.0027 0.0391 0.0003 0.018 0 0.0055 0.0026 0.0018 0.0041 0 0 0 0 0 0.0001
Golgi 0.0087 0 0 0.0027 0.013 0.0007 0.0082 0 0.0024 0.0004 0.0022 0.004 0 0 0 0 0 0
Lipid Particles 0.0034 0 0 0.0016 0.0225 0.0043 0.0161 0 0.0037 0.0149 0.0088 0.0021 0 0 0 0 0 0
Mitochondria 0.0014 0.0001 0.0004 0.0008 0.009 0.0011 0.0062 0.0001 0.0043 0.0073 0.0166 0.0033 0.0001 0.0001 0.0001 0.0001 0.0001 0
None 0.0011 0 0 0.0005 0.003 0 0.0041 0 0.0035 0.0023 0.0007 0.0001 0 0 0 0 0 0
Nuclear Periphery 0.0099 0.0013 0.0023 0.0025 0.0168 0.003 0.0155 0.0015 0.0012 0.0036 0.0045 0.0021 0.0012 0.0011 0.001 0.0005 0.0015 0.0006
Nucleolus 0.0047 0.002 0.0026 0.0016 0.0189 0.0064 0.005 0.0028 0.0015 0.006 0.0506 0.0067 0.002 0.0022 0.0013 0.0014 0.0034 0.0055
Nucleus 0.902 0.9963 0.9871 0.9341 0.797 0.9689 0.8467 0.9951 0.9634 0.9475 0.8477 0.9642 0.9956 0.9927 0.9954 0.9976 0.9941 0.9879
Peroxisomes 0.0021 0 0 0.0014 0.0072 0.0105 0.0136 0 0.0042 0.0013 0.0415 0.0017 0 0 0 0 0 0
Punctate Nuclear 0.0126 0.0001 0.0001 0.0031 0.0072 0.0004 0.0101 0.0002 0.0003 0.0012 0.0034 0.0002 0.0005 0.0006 0.0011 0.0001 0.0003 0.0004
Vacuole 0.0008 0 0.004 0.0001 0.0012 0.0002 0.0027 0 0.0003 0.0037 0.0023 0.0011 0 0 0 0 0.0001 0.0006
Vacuole Periphery 0.0005 0 0.0007 0.0001 0.0014 0.0002 0.0024 0 0.0002 0.0034 0.0009 0.0006 0 0 0 0 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 80.8174 91.413 106.1249 71.57 75.9144 74.1789 67.4573 61.3763 74.58 68.9218
Translational Efficiency 2.0274 2.1707 1.6063 2.3844 2.2393 1.9743 2.0604 2.4638 1.5296 1.6328

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; contains ankyrin (Ank) repeats; YCR051W is not an essential gene
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-5

YCR051W

YCR051W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ycr051w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available