Standard name
Human Ortholog
Description Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway; CPR4 has a paralog, CPR8, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.35 0.5 0.62 0.77 0.82 0.83 0.87 0.9 0.91 0.88 0.86 0.82 0.87 0.95 0.3 0.27 0.3 0 0.1 0.05 0.06 0.12 0.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0.06 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0
Mitochondria 0.11 0 0.07 0 0 0.09 0.05 0 0.07 0.12 0.16 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.14 0 0 0 0 0 0 0 0 0 0 0 0 0 0.25 0.17 0.14 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.59 0.56 0.42 0.45 0.28 0.19 0.18 0.11 0.09 0.1 0.07 0.24 0.14 0 0.61 0.68 0.7 0.9 0.77 0.82 0.71 0.7 0.78
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 2 0 0 2 0 0 0 0 1 1 0 0 0
Bud 0 3 0 2 10 14 11 6 9 34 35 1 1 3 0 0 0 0 2 1 0 1 0
Bud Neck 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 2
Bud Site 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0
Cell Periphery 0 1 0 0 1 1 0 2 1 1 1 0 0 3 0 0 0 0 0 0 0 0 0
Cytoplasm 28 70 82 359 690 818 1044 1028 1142 801 971 28 80 190 87 71 71 2 17 7 1 12 15
Endoplasmic Reticulum 0 2 3 0 1 1 1 1 0 1 2 0 1 2 15 13 6 1 2 9 0 3 1
Endosome 0 4 1 7 17 6 10 21 17 7 4 0 1 0 15 7 6 0 6 2 0 2 4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 1 1 0 1
Mitochondria 9 0 9 5 16 87 61 27 85 110 185 0 1 0 9 4 3 1 2 1 0 4 4
Nucleus 11 2 0 6 1 15 15 17 9 3 7 0 1 0 72 45 33 3 0 0 0 0 1
Nuclear Periphery 0 1 0 0 4 2 11 11 2 0 4 0 0 0 0 2 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 2 1 5 1 1 4 0 1 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 2 0 5 3 2 1 0 0 1 0 0 1 0 1 0 0 0 0
Vac/Vac Membrane 47 79 56 208 240 192 216 126 111 87 81 8 13 3 176 175 164 96 127 125 18 74 118
Unique Cell Count 79 140 132 464 843 986 1202 1141 1250 912 1134 34 92 199 287 259 235 107 166 152 26 107 152
Labelled Cell Count 95 162 151 587 980 1140 1370 1252 1381 1048 1297 37 99 205 376 318 284 107 166 152 26 107 152


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 7.0 5.7 6.3 6.1 4.6 4.2 4.8 3.9 3.9 3.6 6.7 6.6 4.9 8.6 8.4 8.8 7.6 7.4
Std Deviation (1e-4) 1.2 1.8 1.2 1.7 1.5 1.5 1.3 5.5 1.1 0.9 0.8 1.4 2.0 1.0 2.0 1.9 2.0 2.5 1.5
Intensity Change (Log2) 0.14 0.09 -0.29 -0.45 -0.26 -0.54 -0.56 -0.67 0.24 0.21 -0.22 0.59 0.56 0.64 0.42 0.38


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3.5 5.2 5.7 7.4 9.1 10.0 7.6 6.8 2.2 4.1 7.8 -6.2 -6.7 -5.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 1.4 1.3 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 2.2 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 -1.7 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 6.3 5.1 4.5
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.5 -3.2 -6.0 -6.6 -9.8 -11.2 -10.3 -12.3 -2.0 -4.5 -9.5 3.6 4.8 5.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.3488 2.5788 2.3337 2.2982 1.9309 2.2575 0.9201 2.2985 1.9226 1.9538 1.8098 1.9877 -0.6899 0.9234 0.6612 0.2984 0.5667 0.5631
Actin 0.0096 0.0002 0.0096 0.0036 0.0212 0.0008 0.0223 0.0002 0.0075 0.0324 0.005 0.006 0.0171 0.0001 0.0001 0 0 0
Bud 0.001 0.0019 0.0016 0.002 0.0006 0.0041 0.0005 0.0008 0.0009 0.0008 0.0004 0.0005 0.0003 0.0003 0.0004 0.0005 0.0002 0.0002
Bud Neck 0.0024 0.0041 0.0018 0.0016 0.0016 0.0038 0.0014 0.0041 0.0006 0.001 0.0013 0.0016 0.0004 0.0008 0.0003 0.0003 0.0004 0.0011
Bud Periphery 0.0028 0.0014 0.0038 0.0042 0.0008 0.003 0.0009 0.001 0.0016 0.0006 0.0006 0.001 0.0005 0.0005 0.0005 0.0012 0.0001 0.0003
Bud Site 0.0034 0.0143 0.0049 0.0044 0.0022 0.004 0.0018 0.0071 0.0019 0.0021 0.0011 0.0009 0.0014 0.0013 0.0015 0.0005 0.0005 0.0003
Cell Periphery 0.0015 0.0013 0.0011 0.0006 0.0004 0.0009 0.0009 0.002 0.0013 0.0004 0.0007 0.0009 0.0006 0.0007 0.0006 0.0003 0.0002 0.0004
Cytoplasm 0.0859 0.1851 0.1341 0.0345 0.104 0.1266 0.1075 0.1855 0.1572 0.0945 0.1304 0.1598 0.1771 0.2226 0.2267 0.1155 0.0236 0.177
Cytoplasmic Foci 0.057 0.0645 0.0528 0.0555 0.0527 0.0354 0.0587 0.0359 0.0426 0.044 0.0429 0.0352 0.0445 0.0259 0.0315 0.0206 0.0109 0.0187
Eisosomes 0.0003 0.0001 0.0008 0.0001 0.0002 0 0.0002 0.0001 0.0002 0.0003 0 0.0001 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0202 0.0125 0.0168 0.0026 0.0097 0.0086 0.0214 0.0192 0.0275 0.0061 0.0062 0.0158 0.0449 0.0247 0.0491 0.0304 0.0026 0.013
Endosome 0.2927 0.2094 0.2954 0.3744 0.302 0.2334 0.3026 0.2424 0.3158 0.4091 0.3434 0.2661 0.3164 0.2055 0.3042 0.3206 0.3755 0.2628
Golgi 0.0116 0.0037 0.0073 0.0168 0.0119 0.0045 0.0141 0.0055 0.0113 0.0081 0.0079 0.0069 0.0087 0.0025 0.0076 0.0058 0.0032 0.0026
Lipid Particles 0.0197 0.0111 0.0154 0.018 0.0226 0.0111 0.0185 0.013 0.0086 0.0056 0.0115 0.013 0.0151 0.0036 0.0071 0.0047 0.0007 0.0038
Mitochondria 0.0181 0.0025 0.0076 0.0133 0.0064 0.0051 0.0116 0.0027 0.0151 0.0023 0.003 0.0105 0.0042 0.0126 0.0067 0.0233 0.0003 0.0014
None 0.1467 0.1029 0.1044 0.0375 0.051 0.0485 0.1594 0.1098 0.1213 0.0382 0.0336 0.1017 0.1123 0.0749 0.0894 0.0277 0.0002 0.0148
Nuclear Periphery 0.0104 0.0109 0.0129 0.003 0.0088 0.0067 0.0167 0.0105 0.0149 0.0052 0.0048 0.0076 0.0162 0.0139 0.0188 0.0137 0.0037 0.0172
Nucleolus 0.0027 0.0045 0.0039 0.0017 0.0011 0.0019 0.0009 0.0014 0.001 0.0016 0.001 0.001 0.0008 0.0008 0.0007 0.0009 0.0004 0.0005
Nucleus 0.0099 0.0165 0.0063 0.0032 0.0106 0.0116 0.0042 0.0052 0.004 0.0054 0.0039 0.0049 0.0053 0.0063 0.0041 0.0052 0.002 0.0045
Peroxisomes 0.0107 0.0012 0.0058 0.0167 0.02 0.0078 0.0067 0.001 0.0019 0.003 0.022 0.0024 0.0046 0.0002 0.0003 0.0007 0.0001 0.0002
Punctate Nuclear 0.0107 0.0036 0.0027 0.0023 0.0019 0.001 0.0058 0.0009 0.0045 0.0057 0.0015 0.0008 0.0114 0.0006 0.0007 0.0006 0.0001 0.0002
Vacuole 0.2584 0.3273 0.2803 0.368 0.3348 0.4392 0.2243 0.3145 0.2197 0.3028 0.3444 0.325 0.1956 0.3715 0.2168 0.3556 0.5195 0.4172
Vacuole Periphery 0.0242 0.0211 0.0309 0.0362 0.0357 0.0419 0.0195 0.0373 0.0406 0.0308 0.0343 0.0384 0.0227 0.0305 0.033 0.0721 0.0558 0.0639

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 70.9084 69.0566 67.3042 54.2269 56.8059 73.75 93.5317 96.0102 80.2232 56.517
Translational Efficiency 0.993 1.1505 0.8104 0.8462 0.8209 1.0978 0.9273 0.8557 0.8724 1.0102

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway; CPR4 has a paralog, CPR8, that arose from the whole genome duplication
Localization
Cell Percentages vacuole (70%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Cpr4

Cpr4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cpr4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available