Standard name | |
---|---|
Human Ortholog | |
Description | Protein that associates with mitochondrial ribosome; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL027C is not an essential gene |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 4 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 13 | 5 | 7 | 3 | 5 | 1 | 2 | 2 | 13 | 2 | 11 | 14 |
Bud | 6 | 1 | 6 | 8 | 11 | 14 | 22 | 15 | 23 | 25 | 27 | 25 | 1 | 3 | 2 | 5 | 8 | 1 | 1 | 1 | 0 | 2 | 3 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 3 | 1 | 0 | 2 | 0 | 2 | 0 | 1 | 1 | 3 | 3 |
Bud Site | 0 | 0 | 0 | 1 | 1 | 2 | 8 | 8 | 3 | 9 | 13 | 9 | 0 | 0 | 1 | 1 | 0 | 2 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 10 | 5 | 4 | 1 | 4 | 1 | 1 | 2 | 0 | 4 | 5 | 10 | 7 | 17 | 14 | 7 | 0 | 0 | 0 | 1 | 0 | 2 |
Cytoplasm | 18 | 8 | 28 | 28 | 27 | 11 | 23 | 12 | 15 | 6 | 11 | 10 | 89 | 135 | 173 | 27 | 20 | 16 | 1 | 0 | 1 | 12 | 13 | 10 |
Endoplasmic Reticulum | 4 | 6 | 8 | 1 | 0 | 4 | 2 | 0 | 1 | 2 | 0 | 3 | 9 | 8 | 9 | 9 | 12 | 5 | 5 | 0 | 2 | 2 | 1 | 2 |
Endosome | 24 | 0 | 27 | 16 | 50 | 5 | 5 | 10 | 6 | 0 | 2 | 0 | 9 | 23 | 31 | 42 | 38 | 35 | 36 | 13 | 29 | 12 | 19 | 25 |
Golgi | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 32 | 27 | 14 | 7 | 0 | 11 | 1 | 4 | 9 |
Mitochondria | 170 | 68 | 191 | 162 | 270 | 353 | 519 | 472 | 426 | 555 | 430 | 480 | 14 | 17 | 21 | 38 | 23 | 10 | 178 | 69 | 201 | 175 | 294 | 304 |
Nucleus | 3 | 0 | 1 | 0 | 4 | 6 | 16 | 17 | 6 | 13 | 7 | 9 | 1 | 1 | 2 | 10 | 1 | 4 | 0 | 0 | 0 | 3 | 2 | 3 |
Nuclear Periphery | 0 | 1 | 0 | 1 | 0 | 17 | 23 | 17 | 8 | 21 | 6 | 15 | 0 | 0 | 0 | 1 | 1 | 0 | 5 | 0 | 2 | 0 | 0 | 3 |
Nucleolus | 6 | 1 | 0 | 0 | 2 | 4 | 17 | 14 | 15 | 11 | 9 | 7 | 0 | 0 | 0 | 2 | 1 | 0 | 1 | 0 | 1 | 0 | 2 | 1 |
Peroxisomes | 12 | 0 | 34 | 33 | 35 | 3 | 7 | 12 | 11 | 11 | 2 | 4 | 2 | 0 | 5 | 32 | 17 | 18 | 7 | 2 | 24 | 2 | 10 | 13 |
SpindlePole | 13 | 0 | 13 | 12 | 13 | 13 | 15 | 31 | 19 | 19 | 8 | 21 | 7 | 10 | 14 | 17 | 24 | 20 | 4 | 2 | 4 | 9 | 8 | 11 |
Vac/Vac Membrane | 16 | 8 | 15 | 5 | 36 | 21 | 13 | 27 | 20 | 20 | 14 | 24 | 15 | 22 | 15 | 48 | 27 | 27 | 11 | 6 | 1 | 17 | 25 | 22 |
Unique Cell Count | 219 | 75 | 265 | 208 | 348 | 377 | 571 | 527 | 459 | 575 | 449 | 503 | 153 | 225 | 270 | 234 | 178 | 131 | 274 | 107 | 303 | 252 | 410 | 439 |
Labelled Cell Count | 273 | 97 | 337 | 272 | 454 | 454 | 674 | 636 | 554 | 695 | 529 | 611 | 166 | 239 | 289 | 284 | 220 | 160 | 274 | 107 | 303 | 252 | 410 | 439 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.0 | 4.3 | 4.6 | 4.2 | 4.9 | 4.0 | 4.0 | 3.9 | 3.9 | 3.7 | 3.4 | 3.5 | 5.6 | 5.6 | 5.5 | 6.8 | 6.7 | 6.3 | 5.3 | 5.8 | 6.0 |
Std Deviation (1e-4) | 0.8 | 0.5 | 1.4 | 1.8 | 2.3 | 1.3 | 1.6 | 1.2 | 1.5 | 0.9 | 1.0 | 1.1 | 0.8 | 1.1 | 1.0 | 1.3 | 1.4 | 1.3 | 1.0 | 1.2 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.14 | 0.08 | -0.21 | -0.21 | -0.23 | -0.26 | -0.32 | -0.45 | -0.4 | 0.27 | 0.28 | 0.25 | 0.56 | 0.54 | 0.45 | 0.19 | 0.33 | 0.36 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 5.0 | 3.3 | 4.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.6392 | 2.9493 | 3.0099 | 3.0425 | 2.8609 | 3.2166 | 0.399 | 0.7013 | 0.4321 | -0.136 | 0.6145 | 0.6872 | 0.2954 | 0.5406 | 0.0483 | 0.1241 | -0.4523 | -0.0055 |
Actin | 0.013 | 0.009 | 0.0271 | 0.007 | 0.0159 | 0.0251 | 0.0581 | 0.0195 | 0.04 | 0.0587 | 0.0839 | 0.0194 | 0.0493 | 0.0141 | 0.0385 | 0.0108 | 0.0423 | 0.027 |
Bud | 0.0022 | 0.004 | 0.0077 | 0.0023 | 0.006 | 0.0031 | 0.0017 | 0.0036 | 0.0061 | 0.0065 | 0.0121 | 0.0019 | 0.0088 | 0.0055 | 0.0093 | 0.0062 | 0.0047 | 0.0018 |
Bud Neck | 0.0036 | 0.0025 | 0.0053 | 0.0058 | 0.0021 | 0.019 | 0.0035 | 0.0017 | 0.0038 | 0.0093 | 0.0013 | 0.0238 | 0.0041 | 0.0047 | 0.0057 | 0.0017 | 0.0021 | 0.0128 |
Bud Periphery | 0.0049 | 0.0062 | 0.0086 | 0.0065 | 0.0053 | 0.0066 | 0.0033 | 0.0034 | 0.0072 | 0.0057 | 0.0163 | 0.0025 | 0.0075 | 0.0068 | 0.0065 | 0.0083 | 0.0152 | 0.0026 |
Bud Site | 0.0125 | 0.0508 | 0.0228 | 0.005 | 0.0022 | 0.0035 | 0.0126 | 0.04 | 0.0296 | 0.0125 | 0.0059 | 0.0028 | 0.0086 | 0.0554 | 0.024 | 0.0096 | 0.0073 | 0.0019 |
Cell Periphery | 0.0055 | 0.0027 | 0.0044 | 0.0014 | 0.001 | 0.0057 | 0.0106 | 0.0012 | 0.0024 | 0.0011 | 0.002 | 0.0006 | 0.0011 | 0.0012 | 0.0041 | 0.0007 | 0.0087 | 0.0005 |
Cytoplasm | 0.0631 | 0.1374 | 0.1083 | 0.0714 | 0.1003 | 0.0924 | 0.0372 | 0.1445 | 0.1472 | 0.2145 | 0.096 | 0.1476 | 0.0484 | 0.1578 | 0.1147 | 0.1411 | 0.0589 | 0.1346 |
Cytoplasmic Foci | 0.1124 | 0.1304 | 0.1118 | 0.0651 | 0.0939 | 0.1177 | 0.1424 | 0.1381 | 0.1111 | 0.1 | 0.0691 | 0.0963 | 0.107 | 0.1329 | 0.1535 | 0.0909 | 0.1236 | 0.1477 |
Eisosomes | 0.0031 | 0.0014 | 0.0013 | 0.0005 | 0.0003 | 0.0006 | 0.0007 | 0.0002 | 0.0006 | 0.0003 | 0.0013 | 0.0002 | 0.0007 | 0.0027 | 0.0011 | 0.0002 | 0.0018 | 0.0003 |
Endoplasmic Reticulum | 0.0103 | 0.0057 | 0.006 | 0.0079 | 0.0022 | 0.0045 | 0.0105 | 0.0088 | 0.0099 | 0.0039 | 0.0132 | 0.0048 | 0.0158 | 0.0056 | 0.0071 | 0.0068 | 0.0115 | 0.0073 |
Endosome | 0.1735 | 0.1536 | 0.1325 | 0.0936 | 0.2017 | 0.149 | 0.2919 | 0.2582 | 0.2305 | 0.273 | 0.2355 | 0.2036 | 0.218 | 0.232 | 0.1854 | 0.1782 | 0.2419 | 0.243 |
Golgi | 0.0897 | 0.0858 | 0.0692 | 0.0526 | 0.069 | 0.1104 | 0.109 | 0.1428 | 0.0958 | 0.0891 | 0.152 | 0.1721 | 0.101 | 0.1183 | 0.1076 | 0.0955 | 0.0953 | 0.1119 |
Lipid Particles | 0.0418 | 0.0197 | 0.0182 | 0.0121 | 0.0019 | 0.0173 | 0.0346 | 0.0087 | 0.0088 | 0.006 | 0.0426 | 0.0158 | 0.0252 | 0.0212 | 0.0092 | 0.0047 | 0.0123 | 0.0114 |
Mitochondria | 0.297 | 0.3068 | 0.3252 | 0.4706 | 0.3582 | 0.3325 | 0.1688 | 0.153 | 0.2405 | 0.1642 | 0.1979 | 0.2375 | 0.2494 | 0.115 | 0.2561 | 0.3306 | 0.2679 | 0.1961 |
None | 0.0948 | 0.0401 | 0.0883 | 0.1321 | 0.0811 | 0.063 | 0.0339 | 0.0414 | 0.0148 | 0.0025 | 0.0188 | 0.0142 | 0.0644 | 0.0707 | 0.0283 | 0.0796 | 0.0756 | 0.0362 |
Nuclear Periphery | 0.0107 | 0.0018 | 0.0015 | 0.0014 | 0.0004 | 0.0007 | 0.0115 | 0.0026 | 0.0024 | 0.0016 | 0.0022 | 0.0008 | 0.0123 | 0.0024 | 0.0035 | 0.0013 | 0.0019 | 0.002 |
Nucleolus | 0.0022 | 0.0022 | 0.0009 | 0.0009 | 0.0003 | 0.0011 | 0.0014 | 0.0003 | 0.0005 | 0.0005 | 0.0012 | 0.0004 | 0.002 | 0.0025 | 0.0009 | 0.0003 | 0.0005 | 0.0083 |
Nucleus | 0.0056 | 0.002 | 0.0017 | 0.0011 | 0.0006 | 0.0016 | 0.0036 | 0.0012 | 0.0017 | 0.0071 | 0.0013 | 0.0012 | 0.0187 | 0.0032 | 0.0034 | 0.0012 | 0.0011 | 0.0017 |
Peroxisomes | 0.0357 | 0.0217 | 0.0439 | 0.0452 | 0.0389 | 0.0355 | 0.0254 | 0.0122 | 0.0243 | 0.0161 | 0.0336 | 0.0363 | 0.0241 | 0.0193 | 0.0246 | 0.0179 | 0.0086 | 0.0324 |
Punctate Nuclear | 0.0021 | 0.0023 | 0.001 | 0.0004 | 0.0004 | 0.0008 | 0.0175 | 0.001 | 0.002 | 0.0009 | 0.0012 | 0.0005 | 0.0052 | 0.0015 | 0.0045 | 0.0014 | 0.0032 | 0.001 |
Vacuole | 0.0114 | 0.0109 | 0.0107 | 0.0128 | 0.0132 | 0.0064 | 0.0156 | 0.0128 | 0.0144 | 0.0194 | 0.0074 | 0.012 | 0.019 | 0.0198 | 0.0084 | 0.0082 | 0.0093 | 0.0132 |
Vacuole Periphery | 0.0052 | 0.003 | 0.0037 | 0.0043 | 0.005 | 0.0034 | 0.0061 | 0.0048 | 0.0065 | 0.007 | 0.0053 | 0.0057 | 0.0091 | 0.0072 | 0.0036 | 0.0047 | 0.0064 | 0.006 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 16.5991 | 4.6859 | 6.9542 | 15.9724 | 14.7252 | 10.4778 | 16.3852 | 12.791 | 17.2484 | 16.8602 |
Translational Efficiency | 0.4775 | 1.64 | 0.8593 | 0.4273 | 0.4013 | 0.8809 | 0.3422 | 0.6152 | 0.495 | 0.4262 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|