Standard name
Human Ortholog
Description Protein containing a Kruppel-type zinc-finger domain; similar to Stp1p, Stp2p; predicted transcription factor; relative distribution to the nucleus increases upon DNA replication stress; STP4 has a paralog, STP3, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.94 0.9 0.91 0.97 0.95 0.94 0.85 0.88 0.81 0.76 0.69 0.71 0.98 0.99 1.0 – 0.93 0.92 0.68 0.75 0.63 0.84 0.87 0.79
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.09 0.09 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.14 0.09 0.15 0.14 0.1 0.17 0.36 0.21 0.31 0.45 0.55 0.46 0 0 0 – 0 0 0.16 0.09 0.23 0 0 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.06 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 1 4 0 1 0 1 4
Bud 0 1 0 0 1 0 2 0 3 1 3 4 0 1 0 – 0 1 0 0 0 1 3 7
Bud Neck 0 0 0 0 4 2 1 3 0 4 0 2 0 0 0 – 2 0 1 0 0 0 1 3
Bud Site 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 1 1 0 1 2 0 0 0 0 1 0 – 3 4 0 1 0 0 1 1
Cytoplasm 209 82 77 34 107 93 153 120 98 96 82 84 201 286 244 – 263 206 160 87 50 104 202 211
Endoplasmic Reticulum 0 1 0 0 0 1 0 0 0 0 1 0 1 1 0 – 26 20 1 0 1 0 1 3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 2 5 5 3 1 1 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 – 0 0 1 0 0 1 0 0
Mitochondria 30 8 13 5 11 17 66 29 38 57 66 54 3 2 1 – 8 6 38 10 18 5 9 14
Nucleus 0 0 1 0 0 1 2 2 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 1 1 2 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 2 0 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 3 0 1 0 0 0 0 0
SpindlePole 0 1 0 0 0 0 1 0 0 2 0 1 0 0 0 – 1 1 4 3 1 2 3 5
Vac/Vac Membrane 0 1 1 0 0 0 0 0 1 2 0 1 3 1 0 – 16 9 9 4 1 1 2 6
Unique Cell Count 222 91 85 35 113 99 181 136 121 126 119 118 206 289 244 282 223 234 116 80 124 233 267
Labelled Cell Count 239 94 92 39 124 115 227 155 145 163 154 146 209 292 245 324 254 234 116 80 124 233 267


Cytoplasm, Mitochondria, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.1 3.5 3.2 3.6 3.0 3.0 2.2 3.1 2.7 2.3 2.6 2.4 3.7 4.0 3.9 4.2 5.3 5.4 3.6 3.7 4.4
Std Deviation (1e-4) 0.4 0.9 1.2 1.8 1.3 1.9 1.0 1.8 1.6 1.0 1.4 1.3 1.3 1.4 1.2 1.5 2.4 2.3 1.3 1.7 1.5
Intensity Change (Log2) – – – 0.17 -0.06 -0.09 -0.51 -0.04 -0.22 -0.47 -0.27 -0.43 0.21 0.34 0.3 0.42 0.75 0.77 0.17 0.23 0.49


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.266 0.2067 0.3305 -0.1805 0.2541 0.2476 1.2908 1.2292 1.2539 1.0097 1.6597 1.063 2.056 1.0356 1.213 2.4598 1.3995 1.3655
Actin 0.0517 0.0003 0.0171 0 0.0171 0.0041 0.031 0.0003 0.0131 0.0048 0.0222 0.001 0.0442 0.0073 0.0117 0.0247 0.019 0.0074
Bud 0.0038 0.0088 0.0054 0.0001 0.0058 0.0002 0.001 0.005 0.0006 0.0006 0.0021 0.003 0.0007 0.0001 0.0003 0.0005 0.0014 0.0022
Bud Neck 0.0024 0.0008 0.0011 0.0003 0.0005 0.0025 0.0089 0.0015 0.0011 0.0005 0.0006 0.0009 0.0026 0.0001 0.0008 0.0002 0.0001 0.001
Bud Periphery 0.0049 0.0053 0.0109 0.0001 0.009 0.0009 0.0045 0.0025 0.0014 0.0006 0.0047 0.0007 0.0032 0.0001 0.0003 0.0016 0.0049 0.0124
Bud Site 0.0071 0.0224 0.0058 0.0001 0.001 0.0002 0.0083 0.0253 0.0129 0.0008 0.0068 0.0001 0.007 0.0022 0.0058 0.0009 0.0002 0.004
Cell Periphery 0.0006 0.0004 0.0003 0 0.0002 0.0001 0.001 0.0011 0.0014 0.0001 0.0006 0.0001 0.0043 0.0001 0.0002 0.0001 0.0002 0.0002
Cytoplasm 0.0763 0.0707 0.0648 0.0478 0.0472 0.131 0.0184 0.0243 0.0441 0.026 0.0122 0.0676 0.0499 0.0421 0.0557 0.0153 0.022 0.0688
Cytoplasmic Foci 0.0179 0.0043 0.0145 0.0012 0.0066 0.0102 0.028 0.0181 0.0142 0.0171 0.0313 0.0081 0.0263 0.0137 0.0082 0.0086 0.0027 0.004
Eisosomes 0.0007 0.0001 0.0005 0 0.0003 0.0002 0.0022 0.0001 0.0005 0.0001 0.0003 0.0001 0.0012 0.0003 0.002 0.0006 0.0002 0.0001
Endoplasmic Reticulum 0.0032 0.0014 0.0024 0.0004 0.0015 0.0017 0.0036 0.0004 0.0013 0.0011 0.0016 0.0007 0.0083 0.0006 0.0018 0.0013 0.0018 0.0009
Endosome 0.0122 0.0007 0.006 0.0003 0.0054 0.0031 0.0103 0.0009 0.0118 0.0303 0.0411 0.0015 0.0203 0.003 0.0023 0.0113 0.003 0.003
Golgi 0.0062 0.0001 0.003 0 0.0096 0.0005 0.0039 0.0002 0.0068 0.0189 0.0128 0.0002 0.0058 0.0031 0.001 0.0066 0.0121 0.0024
Lipid Particles 0.0093 0.0003 0.0046 0.0001 0.0101 0.0011 0.013 0.0012 0.0059 0.0111 0.0132 0.0005 0.0154 0.0049 0.0035 0.0035 0.0003 0.0052
Mitochondria 0.0062 0.0004 0.0169 0.0003 0.0309 0.0022 0.0109 0.0005 0.0072 0.0081 0.0224 0.0003 0.0032 0.0022 0.0007 0.0097 0.0015 0.0015
None 0.7061 0.8414 0.776 0.891 0.7857 0.7814 0.8126 0.8771 0.8558 0.8492 0.8011 0.8896 0.7534 0.9038 0.8861 0.8749 0.9259 0.8542
Nuclear Periphery 0.0073 0.0013 0.0098 0.0005 0.0018 0.0027 0.0096 0.0003 0.0019 0.0014 0.0012 0.0011 0.012 0.0007 0.002 0.0104 0.0019 0.0045
Nucleolus 0.0017 0.0013 0.0014 0.0007 0.0008 0.0013 0.0015 0.0078 0.0005 0.0005 0.0008 0.0006 0.0011 0.0003 0.0008 0.0007 0.0001 0.0005
Nucleus 0.0573 0.0344 0.0408 0.0561 0.0534 0.0443 0.0034 0.0217 0.0069 0.013 0.002 0.0122 0.0137 0.0083 0.0082 0.0021 0.0007 0.0152
Peroxisomes 0.0104 0.0002 0.005 0 0.0085 0.0005 0.0139 0.0013 0.0076 0.0102 0.0101 0.0055 0.0111 0.0024 0.0048 0.0033 0.0002 0.0015
Punctate Nuclear 0.012 0.0044 0.011 0.0007 0.0022 0.0097 0.0115 0.0038 0.0031 0.0036 0.0042 0.0058 0.0125 0.0043 0.003 0.0214 0.0009 0.0099
Vacuole 0.002 0.001 0.0017 0.0004 0.0014 0.0015 0.0017 0.0063 0.0012 0.0016 0.0064 0.0005 0.0024 0.0003 0.0005 0.0009 0.0005 0.0005
Vacuole Periphery 0.0008 0.0001 0.0011 0.0001 0.0009 0.0004 0.0008 0.0003 0.0005 0.0005 0.0025 0.0001 0.0015 0.0001 0.0001 0.0013 0.0003 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 2.9963 0.6696 1.4907 3.1294 0.9712 2.9947 1.1849 3.8843 2.2661 1.9116
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –
Standard name
Human Ortholog
Description Protein containing a Kruppel-type zinc-finger domain; similar to Stp1p, Stp2p; predicted transcription factor; relative distribution to the nucleus increases upon DNA replication stress; STP4 has a paralog, STP3, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (50%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Stp4

Stp4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Stp4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available