Standard name | |
---|---|
Human Ortholog | |
Description | Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29B has a paralog, RPS29A, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 2 | – | 21 | 16 | 21 | 12 | 24 | – | – | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 2 | 0 | 0 | 0 | – | 0 | 2 | 1 | 1 | 2 | – | – | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 1 | 4 | 5 | – | 13 | 20 | 15 | 11 | 25 | – | – | – | 4 | 2 | 8 | 0 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 18 | 22 | 121 | 150 | 235 | 131 | – | 207 | 193 | 177 | 106 | 156 | – | – | – | 166 | 295 | 333 | 49 | 46 | 121 | 2 | 10 | 18 |
Endoplasmic Reticulum | 0 | 0 | 1 | 1 | 9 | 6 | – | 44 | 46 | 34 | 28 | 34 | – | – | – | 7 | 12 | 9 | 0 | 1 | 2 | 0 | 0 | 1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 11 | 11 | 6 | 7 | 3 | – | – | – | 0 | 2 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 2 | 1 | 1 | 4 | – | 18 | 24 | 36 | 34 | 55 | – | – | – | 0 | 3 | 3 | 0 | 0 | 2 | 0 | 0 | 0 |
Nucleus | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 3 | 2 | 2 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 1 | 1 | 0 | – | 0 | 0 | 0 | 1 | 0 | – | – | – | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 1 | 1 | – | 5 | 2 | 2 | 0 | 3 | – | – | – | 4 | 14 | 4 | 0 | 0 | 1 | 0 | 0 | 0 |
Unique Cell Count | 18 | 22 | 124 | 153 | 249 | 142 | 288 | 275 | 248 | 167 | 247 | 173 | 313 | 349 | 51 | 51 | 132 | 3 | 11 | 22 | ||||
Labelled Cell Count | 19 | 22 | 127 | 154 | 252 | 149 | 320 | 315 | 295 | 202 | 304 | 181 | 329 | 367 | 51 | 51 | 132 | 3 | 11 | 22 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 53.7 | 79.6 | 33.6 | 46.8 | 41.3 | 29.9 | – | 26.8 | 25.2 | 24.9 | 23.0 | 22.9 | – | – | – | 71.9 | 65.1 | 73.4 | – | – | – |
Std Deviation (1e-4) | 20.3 | 12.8 | 8.6 | 12.8 | 9.0 | 7.2 | – | 5.8 | 6.0 | 5.7 | 4.9 | 5.3 | – | – | – | 24.3 | 24.5 | 23.9 | – | – | – |
Intensity Change (Log2) | – | – | – | 0.48 | 0.3 | -0.17 | – | -0.33 | -0.41 | -0.44 | -0.55 | -0.55 | – | – | – | 1.1 | 0.95 | 1.13 | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 3.1 | 0 | 3.3 | 3.0 | 3.6 | – | – | – | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 2.7 | 2.3 | 2.5 | 3.3 | – | – | – | 0 | 0 | 0 |
Cytoplasm | 0.3 | -1.4 | -1.9 | – | -5.9 | -6.2 | -6.0 | -6.9 | -7.2 | – | – | – | -0.8 | -1.5 | -1.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 4.3 | 4.6 | 4.0 | 4.5 | 4.0 | – | – | – | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | – | 2.0 | 2.7 | 3.9 | 4.8 | 5.2 | – | – | – | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 51.318 | 87.6994 | 74.8865 | 68.7191 | 59.8384 | 79.1386 | 71.6824 | 91.6732 | 81.8798 | 85.8267 | 72.8379 | 72.6392 | 49.5908 | 105.112 | 95.366 | 91.026 | 97.0089 | 87.7108 |
Actin | 0.0168 | 0.0008 | 0.0129 | 0.002 | 0.0003 | 0.0023 | 0.0051 | 0.0003 | 0.0012 | 0.0008 | 0.0007 | 0.0008 | 0.043 | 0.0012 | 0.0137 | 0.0004 | 0 | 0.0126 |
Bud | 0.0058 | 0.0018 | 0.0083 | 0.0025 | 0.0023 | 0.0012 | 0.0014 | 0.0004 | 0.0009 | 0.0021 | 0.0002 | 0.0011 | 0.0049 | 0.0008 | 0.0031 | 0.0016 | 0 | 0.0025 |
Bud Neck | 0.0011 | 0.0004 | 0.001 | 0.0022 | 0.0004 | 0.0085 | 0.0004 | 0.0003 | 0.0007 | 0.0009 | 0.0006 | 0.0011 | 0.0005 | 0.0004 | 0.0004 | 0.0004 | 0.0005 | 0.001 |
Bud Periphery | 0.0096 | 0.0014 | 0.0049 | 0.0017 | 0.0025 | 0.0012 | 0.0015 | 0.0001 | 0.0005 | 0.0013 | 0.0001 | 0.0005 | 0.0043 | 0.0003 | 0.0025 | 0.0008 | 0 | 0.0016 |
Bud Site | 0.0069 | 0.0016 | 0.0021 | 0.0014 | 0.0008 | 0.0002 | 0.0015 | 0.0003 | 0.0006 | 0.0001 | 0 | 0.0001 | 0.0031 | 0.0011 | 0.0041 | 0.0004 | 0 | 0.0003 |
Cell Periphery | 0.0128 | 0.0065 | 0.0102 | 0.0026 | 0.0058 | 0.0045 | 0.0069 | 0.0008 | 0.0019 | 0.0008 | 0.0004 | 0.0005 | 0.0053 | 0.0071 | 0.0081 | 0.0021 | 0 | 0.0008 |
Cytoplasm | 0.6019 | 0.9212 | 0.8177 | 0.7224 | 0.676 | 0.7714 | 0.7789 | 0.9835 | 0.9117 | 0.8932 | 0.8337 | 0.8757 | 0.5954 | 0.9553 | 0.8753 | 0.6562 | 0.9753 | 0.7888 |
Cytoplasmic Foci | 0.0115 | 0.0013 | 0.0085 | 0.0157 | 0.0017 | 0.0066 | 0.002 | 0.0013 | 0.0027 | 0.0036 | 0.0038 | 0.0072 | 0.0203 | 0.0011 | 0.0064 | 0.0091 | 0.007 | 0.0071 |
Eisosomes | 0.0004 | 0 | 0.0003 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0011 | 0 | 0.0002 | 0.0001 | 0 | 0.0005 |
Endoplasmic Reticulum | 0.0549 | 0.0072 | 0.0298 | 0.0183 | 0.0131 | 0.0529 | 0.0365 | 0.0039 | 0.0248 | 0.0061 | 0.013 | 0.0113 | 0.0378 | 0.0028 | 0.0051 | 0.0025 | 0.0017 | 0.0268 |
Endosome | 0.0514 | 0.0022 | 0.0112 | 0.0427 | 0.0084 | 0.0501 | 0.013 | 0.0017 | 0.01 | 0.022 | 0.0223 | 0.0125 | 0.0166 | 0.0019 | 0.014 | 0.0312 | 0.0057 | 0.0346 |
Golgi | 0.0073 | 0.0004 | 0.0043 | 0.0057 | 0.0003 | 0.0074 | 0.002 | 0.0003 | 0.0012 | 0.0008 | 0.0016 | 0.001 | 0.0051 | 0.0007 | 0.003 | 0.0009 | 0.0001 | 0.0097 |
Lipid Particles | 0.0044 | 0.0006 | 0.0016 | 0.0038 | 0.0008 | 0.0021 | 0.0014 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0.0002 | 0.0056 | 0.0004 | 0.0005 | 0.0018 | 0.0001 | 0.0004 |
Mitochondria | 0.0176 | 0.0017 | 0.0074 | 0.0078 | 0.0001 | 0.0076 | 0.0154 | 0.0002 | 0.0011 | 0.0008 | 0.0007 | 0.0013 | 0.0385 | 0.0003 | 0.0086 | 0.001 | 0 | 0.075 |
None | 0.0058 | 0.0005 | 0.0032 | 0.0038 | 0.0008 | 0.0008 | 0.0013 | 0.0001 | 0.0003 | 0.0003 | 0.0001 | 0.003 | 0.0139 | 0.0002 | 0.0019 | 0.0076 | 0.0001 | 0.0013 |
Nuclear Periphery | 0.0519 | 0.0023 | 0.0083 | 0.0414 | 0.0402 | 0.0163 | 0.0275 | 0.0006 | 0.0063 | 0.0054 | 0.0033 | 0.0352 | 0.0433 | 0.0017 | 0.0031 | 0.007 | 0.0021 | 0.0082 |
Nucleolus | 0.0021 | 0.0008 | 0.0059 | 0.0007 | 0.0004 | 0.0001 | 0.0007 | 0 | 0.0001 | 0 | 0 | 0.0003 | 0.0063 | 0.0001 | 0.0003 | 0.0012 | 0 | 0.0003 |
Nucleus | 0.0219 | 0.0083 | 0.0051 | 0.0071 | 0.0394 | 0.0028 | 0.0396 | 0.0005 | 0.0029 | 0.0017 | 0.0006 | 0.004 | 0.0976 | 0.0017 | 0.0027 | 0.005 | 0.0013 | 0.0031 |
Peroxisomes | 0.001 | 0.0001 | 0.0003 | 0.0008 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.001 | 0 | 0.0004 | 0.0001 | 0 | 0.0002 |
Punctate Nuclear | 0.0019 | 0 | 0.0004 | 0.001 | 0.0005 | 0.0002 | 0.0003 | 0 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.018 | 0 | 0.0001 | 0.0004 | 0.0001 | 0.0003 |
Vacuole | 0.0911 | 0.0358 | 0.0471 | 0.0909 | 0.1625 | 0.0388 | 0.0465 | 0.0049 | 0.0267 | 0.0459 | 0.0362 | 0.0173 | 0.0259 | 0.0213 | 0.0401 | 0.1855 | 0.0047 | 0.0139 |
Vacuole Periphery | 0.0219 | 0.005 | 0.0095 | 0.0254 | 0.0437 | 0.0248 | 0.0176 | 0.0007 | 0.0056 | 0.0141 | 0.0828 | 0.027 | 0.0125 | 0.0016 | 0.0063 | 0.0847 | 0.0012 | 0.011 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 638.8131 | 496.0722 | 843.2218 | 831.6002 | 991.3891 | 644.9085 | 648.8438 | 553.0659 | 550.6797 | 712.8692 |
Translational Efficiency | 3.9587 | 4.9493 | 2.9509 | 2.1746 | 3.1947 | 4.9834 | 3.5235 | 3.6918 | 4.6963 | 3.9949 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|