Standard name
Human Ortholog
Description Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29B has a paralog, RPS29A, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0.07 0.06 0.08 0.07 0.1 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0.05 0.07 0.06 0.07 0.1 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 1.0 0.98 0.98 0.94 0.92 0.72 0.7 0.71 0.63 0.63 0.96 0.94 0.95 0.97 0.91 0.92 1.0 0.96 0.84
Endoplasmic Reticulum 0 0 0 0 0 0 0.15 0.17 0.14 0.17 0.14 0 0 0 0 0 0 0 0 0.06
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.06 0.09 0.15 0.2 0.22 0 0 0 0 0 0 0 0 0
Nucleus 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 2 21 16 21 12 24 0 0 1 0 0 0 0 0 0
Bud 0 0 2 0 0 0 0 2 1 1 2 0 1 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 1 1 4 5 13 20 15 11 25 4 2 8 0 0 1 0 0 0
Cytoplasm 18 22 121 150 235 131 207 193 177 106 156 166 295 333 49 46 121 2 10 18
Endoplasmic Reticulum 0 0 1 1 9 6 44 46 34 28 34 7 12 9 0 1 2 0 0 1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 11 11 6 7 3 0 2 6 0 0 0 0 0 0
Mitochondria 0 0 2 1 1 4 18 24 36 34 55 0 3 3 0 0 2 0 0 0
Nucleus 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 1 3 2 2 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 1 0 0 0 0 1 0 0 0 2 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 1 1 5 2 2 0 3 4 14 4 0 0 1 0 0 0
Unique Cell Count 18 22 124 153 249 142 288 275 248 167 247 173 313 349 51 51 132 3 11 22
Labelled Cell Count 19 22 127 154 252 149 320 315 295 202 304 181 329 367 51 51 132 3 11 22


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 53.7 79.6 33.6 46.8 41.3 29.9 26.8 25.2 24.9 23.0 22.9 71.9 65.1 73.4
Std Deviation (1e-4) 20.3 12.8 8.6 12.8 9.0 7.2 5.8 6.0 5.7 4.9 5.3 24.3 24.5 23.9
Intensity Change (Log2) 0.48 0.3 -0.17 -0.33 -0.41 -0.44 -0.55 -0.55 1.1 0.95 1.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 3.1 0 3.3 3.0 3.6 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 2.7 2.3 2.5 3.3 0 0 0
Cytoplasm 0.3 -1.4 -1.9 -5.9 -6.2 -6.0 -6.9 -7.2 -0.8 -1.5 -1.1
Endoplasmic Reticulum 0 0 0 4.3 4.6 4.0 4.5 4.0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 2.0 2.7 3.9 4.8 5.2 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 51.318 87.6994 74.8865 68.7191 59.8384 79.1386 71.6824 91.6732 81.8798 85.8267 72.8379 72.6392 49.5908 105.112 95.366 91.026 97.0089 87.7108
Actin 0.0168 0.0008 0.0129 0.002 0.0003 0.0023 0.0051 0.0003 0.0012 0.0008 0.0007 0.0008 0.043 0.0012 0.0137 0.0004 0 0.0126
Bud 0.0058 0.0018 0.0083 0.0025 0.0023 0.0012 0.0014 0.0004 0.0009 0.0021 0.0002 0.0011 0.0049 0.0008 0.0031 0.0016 0 0.0025
Bud Neck 0.0011 0.0004 0.001 0.0022 0.0004 0.0085 0.0004 0.0003 0.0007 0.0009 0.0006 0.0011 0.0005 0.0004 0.0004 0.0004 0.0005 0.001
Bud Periphery 0.0096 0.0014 0.0049 0.0017 0.0025 0.0012 0.0015 0.0001 0.0005 0.0013 0.0001 0.0005 0.0043 0.0003 0.0025 0.0008 0 0.0016
Bud Site 0.0069 0.0016 0.0021 0.0014 0.0008 0.0002 0.0015 0.0003 0.0006 0.0001 0 0.0001 0.0031 0.0011 0.0041 0.0004 0 0.0003
Cell Periphery 0.0128 0.0065 0.0102 0.0026 0.0058 0.0045 0.0069 0.0008 0.0019 0.0008 0.0004 0.0005 0.0053 0.0071 0.0081 0.0021 0 0.0008
Cytoplasm 0.6019 0.9212 0.8177 0.7224 0.676 0.7714 0.7789 0.9835 0.9117 0.8932 0.8337 0.8757 0.5954 0.9553 0.8753 0.6562 0.9753 0.7888
Cytoplasmic Foci 0.0115 0.0013 0.0085 0.0157 0.0017 0.0066 0.002 0.0013 0.0027 0.0036 0.0038 0.0072 0.0203 0.0011 0.0064 0.0091 0.007 0.0071
Eisosomes 0.0004 0 0.0003 0.0001 0 0 0.0001 0 0 0 0 0 0.0011 0 0.0002 0.0001 0 0.0005
Endoplasmic Reticulum 0.0549 0.0072 0.0298 0.0183 0.0131 0.0529 0.0365 0.0039 0.0248 0.0061 0.013 0.0113 0.0378 0.0028 0.0051 0.0025 0.0017 0.0268
Endosome 0.0514 0.0022 0.0112 0.0427 0.0084 0.0501 0.013 0.0017 0.01 0.022 0.0223 0.0125 0.0166 0.0019 0.014 0.0312 0.0057 0.0346
Golgi 0.0073 0.0004 0.0043 0.0057 0.0003 0.0074 0.002 0.0003 0.0012 0.0008 0.0016 0.001 0.0051 0.0007 0.003 0.0009 0.0001 0.0097
Lipid Particles 0.0044 0.0006 0.0016 0.0038 0.0008 0.0021 0.0014 0.0001 0.0004 0.0001 0.0002 0.0002 0.0056 0.0004 0.0005 0.0018 0.0001 0.0004
Mitochondria 0.0176 0.0017 0.0074 0.0078 0.0001 0.0076 0.0154 0.0002 0.0011 0.0008 0.0007 0.0013 0.0385 0.0003 0.0086 0.001 0 0.075
None 0.0058 0.0005 0.0032 0.0038 0.0008 0.0008 0.0013 0.0001 0.0003 0.0003 0.0001 0.003 0.0139 0.0002 0.0019 0.0076 0.0001 0.0013
Nuclear Periphery 0.0519 0.0023 0.0083 0.0414 0.0402 0.0163 0.0275 0.0006 0.0063 0.0054 0.0033 0.0352 0.0433 0.0017 0.0031 0.007 0.0021 0.0082
Nucleolus 0.0021 0.0008 0.0059 0.0007 0.0004 0.0001 0.0007 0 0.0001 0 0 0.0003 0.0063 0.0001 0.0003 0.0012 0 0.0003
Nucleus 0.0219 0.0083 0.0051 0.0071 0.0394 0.0028 0.0396 0.0005 0.0029 0.0017 0.0006 0.004 0.0976 0.0017 0.0027 0.005 0.0013 0.0031
Peroxisomes 0.001 0.0001 0.0003 0.0008 0 0.0001 0.0001 0 0.0001 0.0001 0 0.0001 0.001 0 0.0004 0.0001 0 0.0002
Punctate Nuclear 0.0019 0 0.0004 0.001 0.0005 0.0002 0.0003 0 0.0001 0.0001 0 0.0002 0.018 0 0.0001 0.0004 0.0001 0.0003
Vacuole 0.0911 0.0358 0.0471 0.0909 0.1625 0.0388 0.0465 0.0049 0.0267 0.0459 0.0362 0.0173 0.0259 0.0213 0.0401 0.1855 0.0047 0.0139
Vacuole Periphery 0.0219 0.005 0.0095 0.0254 0.0437 0.0248 0.0176 0.0007 0.0056 0.0141 0.0828 0.027 0.0125 0.0016 0.0063 0.0847 0.0012 0.011

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 638.8131 496.0722 843.2218 831.6002 991.3891 644.9085 648.8438 553.0659 550.6797 712.8692
Translational Efficiency 3.9587 4.9493 2.9509 2.1746 3.1947 4.9834 3.5235 3.6918 4.6963 3.9949

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29B has a paralog, RPS29A, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Rps29b

Rps29b


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rps29b-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available