Standard name
Human Ortholog
Description Ribosomal stalk protein P1 alpha; involved in the interaction between translational elongation factors and the ribosome; free (non-ribosomal) P1 stimulates the phosphorylation of the eIF2 alpha subunit (Sui2p) by Gcn2p; accumulation of P1 in the cytoplasm is regulated by phosphorylation and interaction with the P2 stalk component

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0.07 0.05 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.95 0.99 0.96 0.98 0.93 0.9 0.83 0.9 0.84 1.0 0.97 0.99 1.0 0.95 1.0 0.96 0.94 0.84 0.94 0.95 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0.05 0 0.14 0.08 0.1 0 0 0 0 0.11 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 2 2 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 1 0 0 0 2 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1 0 0 0 2 0 7 10 8 3 6 0 0 2 0 5 0 0 0 2 0 0 0
Cytoplasm 124 66 72 170 153 135 212 130 141 115 122 118 177 173 35 63 23 129 86 60 52 118 137
Endoplasmic Reticulum 1 0 0 0 2 3 12 6 23 10 14 0 2 0 1 7 1 0 0 1 0 1 3
Endosome 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 1 0 1 0 0 0 0 0 2 0 0 0 2 1 0 0 0 2 0 0 0
Nucleus 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Vac/Vac Membrane 0 1 1 0 0 0 0 1 0 0 0 0 2 0 0 0 0 1 0 1 1 0 1
Unique Cell Count 125 67 76 172 159 138 229 145 169 128 146 118 182 174 35 66 23 135 92 72 56 125 146
Labelled Cell Count 126 67 76 172 161 138 235 151 172 130 146 118 183 175 38 76 24 135 92 72 56 125 146


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 294.8 311.4 188.4 272.8 237.9 191.8 153.5 133.7 128.4 111.2 109.1 306.0 300.3 287.5 318.8 302.9 292.7 294.2 339.1 336.0
Std Deviation (1e-4) 59.5 103.5 79.6 72.6 76.3 59.2 42.5 35.1 33.2 31.6 32.5 67.1 73.4 56.7 126.2 109.0 106.1 82.2 85.3 85.0
Intensity Change (Log2) 0.53 0.34 0.03 -0.3 -0.5 -0.55 -0.76 -0.79 0.7 0.67 0.61 0.76 0.68 0.64 0.64 0.85 0.83


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.9 0.5 1.2 -0.6 -1.3 -2.4 -1.2 -2.4 2.5 1.0 2.4 1.4 0.2 1.1
Endoplasmic Reticulum 0 0 0 0 0 3.4 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 221.8363 341.0973 298.6163 234.3487 224.3048 213.1921 198.6183 343.4382 281.3251 281.6279 266.4788 310.0589 263.0425 414.4092 359.8285 366.6771 308.5747 394.2698
Actin 0.013 0.0015 0.0039 0.0691 0.0084 0.0239 0.0278 0.0007 0.0084 0.001 0.0104 0.0035 0.0398 0.0006 0.0051 0.0006 0.011 0.0111
Bud 0.0044 0.0049 0.0083 0.0044 0.006 0.0173 0.0044 0.0029 0.0051 0.0059 0.0114 0.0052 0.0059 0.0032 0.0038 0.0038 0.0074 0.0051
Bud Neck 0.0011 0.0011 0.0028 0.0012 0.0016 0.0026 0.0019 0.0006 0.0011 0.0018 0.0023 0.0015 0.0024 0.0006 0.0012 0.0016 0.001 0.0019
Bud Periphery 0.0044 0.0025 0.0057 0.003 0.0082 0.0103 0.0056 0.001 0.003 0.0027 0.0103 0.0023 0.0046 0.0011 0.0015 0.0011 0.0026 0.0036
Bud Site 0.0014 0.0031 0.0127 0.0068 0.002 0.0044 0.006 0.0019 0.003 0.0019 0.0013 0.0012 0.011 0.0022 0.0022 0.0004 0.001 0.0012
Cell Periphery 0.0532 0.0247 0.0501 0.0304 0.007 0.007 0.0344 0.0188 0.0498 0.0562 0.0075 0.0313 0.0582 0.0234 0.0206 0.0092 0.0058 0.0038
Cytoplasm 0.4262 0.7685 0.613 0.3524 0.3248 0.3932 0.4399 0.8478 0.5694 0.5163 0.456 0.5817 0.5004 0.8394 0.7859 0.6286 0.6098 0.6272
Cytoplasmic Foci 0.0424 0.0025 0.0033 0.0078 0.0558 0.0789 0.0034 0.0013 0.0143 0.0027 0.0063 0.0075 0.004 0.0008 0.0029 0.0012 0.0118 0.0069
Eisosomes 0.0036 0.0002 0.0004 0.0079 0.0007 0.0016 0.0248 0.0004 0.0049 0.0008 0.0001 0.0004 0.0093 0.0001 0.0075 0 0.0018 0.0006
Endoplasmic Reticulum 0.0199 0.0024 0.0049 0.0058 0.0372 0.0063 0.024 0.0017 0.0029 0.0018 0.0081 0.0035 0.006 0.0022 0.0012 0.0003 0.004 0.0088
Endosome 0.0094 0.002 0.0053 0.0142 0.0787 0.0739 0.014 0.0018 0.0058 0.0057 0.0321 0.0073 0.004 0.0009 0.0025 0.0021 0.0088 0.004
Golgi 0.0037 0.0004 0.0017 0.0169 0.0442 0.0157 0.0029 0.0003 0.0027 0.0003 0.0058 0.0013 0.002 0.0001 0.0014 0.0001 0.0039 0.0011
Lipid Particles 0.0104 0.0009 0.0028 0.0059 0.0114 0.015 0.004 0.0008 0.0059 0.0026 0.0014 0.0025 0.0046 0.0005 0.0026 0.0006 0.0057 0.0016
Mitochondria 0.0802 0.0149 0.0184 0.0943 0.0272 0.0675 0.0676 0.0079 0.0601 0.0091 0.0801 0.1376 0.0793 0.0027 0.0188 0.0019 0.0868 0.1322
None 0.0094 0.0018 0.0025 0.0088 0.0098 0.0455 0.0087 0.0012 0.0077 0.0022 0.0037 0.0042 0.0088 0.0014 0.0033 0.001 0.0128 0.0084
Nuclear Periphery 0.0468 0.0061 0.0154 0.0205 0.0277 0.0301 0.0706 0.0063 0.0068 0.0127 0.0254 0.0322 0.0662 0.008 0.0089 0.0128 0.0123 0.0327
Nucleolus 0.0314 0.0092 0.0189 0.0703 0.0093 0.0114 0.0142 0.0024 0.0146 0.0278 0.002 0.0208 0.0492 0.0021 0.0085 0.0055 0.0071 0.0096
Nucleus 0.0502 0.0238 0.0303 0.0377 0.0261 0.0279 0.0386 0.0136 0.0171 0.03 0.015 0.0406 0.0459 0.0172 0.0203 0.057 0.0259 0.0293
Peroxisomes 0.0099 0.0005 0.0009 0.0047 0.0061 0.0032 0.0013 0.0003 0.0015 0.0008 0.0007 0.0022 0.0016 0.0002 0.0005 0.0002 0.0011 0.0014
Punctate Nuclear 0.0014 0.0001 0.0002 0.0004 0.0013 0.0024 0.002 0.0001 0.0006 0.0001 0.002 0.0005 0.0009 0.0001 0.0002 0.0001 0.0011 0.0007
Vacuole 0.124 0.0982 0.1449 0.1495 0.1737 0.0595 0.1273 0.0705 0.1547 0.2496 0.1722 0.0534 0.0575 0.077 0.0765 0.1694 0.0883 0.059
Vacuole Periphery 0.0536 0.0307 0.0535 0.0879 0.1329 0.1024 0.0764 0.0176 0.0604 0.0679 0.1457 0.0591 0.0387 0.0162 0.0247 0.1024 0.09 0.0498

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3229.4345 3917.8493 5191.9408 2420.0662 3638.9245 4624.175 2846.7229 2401.0273 3508.7395 1984.2905
Translational Efficiency 1.3409 1.2719 1.0769 3.5519 1.7141 0.8789 1.4058 2.7938 1.3226 1.9647

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Ribosomal stalk protein P1 alpha; involved in the interaction between translational elongation factors and the ribosome; free (non-ribosomal) P1 stimulates the phosphorylation of the eIF2 alpha subunit (Sui2p) by Gcn2p; accumulation of P1 in the cytoplasm is regulated by phosphorylation and interaction with the P2 stalk component
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Rpp1a

Rpp1a


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpp1a-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available