Standard name
Human Ortholog
Description A cargo receptor protein for Pma1p; works with Psg1p to promote the transport and maturation of Pma1p; localizes to the ER and COPII vesicles; a non-essential protein
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.05 0.09 0.09 0.07 – 0.07 0 0 0 0 0 0 0.05 0.13 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.05 0 0 0 0 – 0 0.05 0 0.05 0.07 0.06 0.09 0.15 0.29 0.1 0.28 0 0 0 0 0 0
Cytoplasm 0.08 0.14 0.12 0.1 0.15 0.15 – 0.21 0.23 0.33 0.28 0.25 0.12 0.11 0.09 0.29 0.38 0.34 0 0 0 0.06 0 0.06
Endoplasmic Reticulum 0.89 0.88 0.78 0.83 0.78 0.68 – 0.41 0.39 0.13 0.27 0.33 0.84 0.84 0.83 0 0 0.06 0.93 0.91 0.83 0.82 0.83 0.77
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0.06 0 – 0 0 0 0 0 0 0 0 0.26 0.3 0.25 0 0 0 0 0 0
Mitochondria 0 0 0.08 0.22 0.06 0.24 – 0.19 0.35 0.34 0.38 0.4 0 0 0 0.09 0.05 0.05 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0.05 – 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0 0.1 0 0 0.07 – 0.21 0.17 0.28 0.15 0.12 0 0 0 0.09 0.22 0.07 0 0 0 0.05 0.06 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 14 4 6 26 38 31 – 43 7 22 14 16 8 15 22 23 1 2 4 2 0 0 6 5
Bud 5 2 2 1 12 3 – 15 17 33 17 13 0 0 2 4 1 5 3 0 0 0 1 2
Bud Neck 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 2
Bud Site 0 0 0 0 0 0 – 1 0 1 0 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 16 10 5 6 15 15 – 18 23 8 17 31 20 32 68 51 12 34 0 0 1 1 1 6
Cytoplasm 39 28 14 27 61 65 – 132 118 180 102 119 36 39 39 50 47 41 11 8 5 6 12 18
Endoplasmic Reticulum 440 180 93 227 321 288 – 260 198 74 99 158 261 287 372 4 1 7 473 193 123 94 303 249
Endosome 0 0 0 0 1 5 – 15 4 11 8 4 0 0 0 0 0 0 1 0 0 1 4 1
Golgi 3 2 0 5 25 3 – 3 0 0 0 0 2 1 1 46 37 30 3 0 1 1 2 1
Mitochondria 3 0 10 60 26 100 – 118 174 190 141 190 1 2 2 16 6 6 2 1 3 1 3 4
Nucleus 2 0 0 1 1 1 – 3 4 4 2 4 0 0 1 2 0 0 0 0 0 0 0 1
Nuclear Periphery 3 0 0 11 5 21 – 22 31 10 10 8 1 2 5 2 1 0 0 0 3 0 2 1
Nucleolus 0 0 0 0 0 0 – 2 0 3 1 1 0 0 0 0 2 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 – 2 1 2 1 0 0 1 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 24 2 12 2 11 28 – 135 84 153 55 59 5 4 8 16 27 9 3 2 5 5 23 25
Unique Cell Count 494 204 119 274 412 425 628 503 551 367 473 310 341 447 174 124 120 508 213 148 115 365 324
Labelled Cell Count 549 228 142 366 516 561 770 661 692 467 605 334 383 520 214 135 134 508 213 148 115 365 324


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 17.5 22.1 10.0 12.8 12.0 9.6 – 8.7 7.5 7.7 7.7 7.1 18.7 17.5 16.5 33.9 31.2 30.7 15.8 17.9 16.6
Std Deviation (1e-4) 3.2 4.1 2.0 2.5 2.2 1.9 – 2.0 1.9 3.7 4.0 2.1 3.8 3.4 2.8 10.5 9.9 8.7 3.4 3.7 4.0
Intensity Change (Log2) – – – 0.35 0.26 -0.07 – -0.21 -0.42 -0.37 -0.38 -0.51 0.9 0.8 0.72 1.76 1.64 1.61 0.66 0.83 0.73


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.5 1.5 0.9 – 0.7 0 0 0 0 0 -0.3 -0.1 2.3 0 0
Bud 0 0 0 – 0 0 1.9 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0.2 0 0.2 1.0 0.9 1.8 3.2 5.4 1.7 5.0
Cytoplasm -0.6 0.8 1.0 – 2.3 2.8 4.6 3.6 3.1 0 -0.1 -1.0 3.5 4.7 4.1
Endoplasmic Reticulum 1.1 -0.1 -2.2 – -7.4 -7.6 -14.8 -9.9 -8.8 1.5 1.5 1.3 -13.5 -12.4 -11.3
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 2.8 0 – 0 0 0 0 0 0 0 0 6.1 6.5 5.8
Mitochondria 3.2 -0.8 3.6 – 2.8 5.6 5.6 6.1 6.5 0 0 0 0.2 -1.1 -1.1
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 2.8 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 -3.5 -1.3 – 2.9 1.8 4.1 1.3 0.7 0 0 0 -0.3 2.5 -0.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 13.7332 16.3112 14.9625 12.8306 11.1638 14.568 6.4968 15.506 11.154 11.4656 17.7896 12.813 16.7406 19.0147 16.8133 15.2163 17.0439 19.0283
Actin 0.0155 0.0008 0.0084 0.046 0.0272 0.0026 0.0768 0.0017 0.063 0.0867 0.0002 0.0058 0.0193 0.0003 0.0074 0.0121 0.0075 0.0006
Bud 0.0005 0 0.0001 0.0004 0.0008 0.0001 0.0016 0.0001 0.0031 0.0022 0 0.0016 0.0004 0 0.0012 0.0043 0.0017 0
Bud Neck 0.0004 0.0005 0.0015 0.0024 0.0007 0.0008 0.0013 0.0007 0.003 0.0114 0.0004 0.0005 0.0006 0.0001 0.0002 0.0018 0.0004 0.0003
Bud Periphery 0.0009 0.0001 0.0002 0.0008 0.0014 0.0002 0.0024 0.0001 0.0048 0.0036 0 0.0034 0.0005 0 0.0006 0.0084 0.003 0.0001
Bud Site 0.0032 0.0004 0.0004 0.0009 0.0032 0.0001 0.0036 0.0009 0.0102 0.0216 0 0.0009 0.0038 0.0001 0.0008 0.0028 0.0015 0
Cell Periphery 0.0012 0.0059 0.0018 0.0019 0.0009 0.0008 0.0008 0.0067 0.0034 0.0022 0.0002 0.0006 0.0004 0.0016 0.001 0.0012 0.0004 0.0004
Cytoplasm 0.0183 0.0416 0.0259 0.0512 0.0186 0.0159 0.0712 0.1165 0.0471 0.0172 0.0005 0.0273 0.0172 0.0293 0.0233 0.04 0.0159 0.007
Cytoplasmic Foci 0.0119 0.0014 0.0019 0.0033 0.0142 0.0015 0.0227 0.0017 0.0176 0.0039 0 0.0039 0.0209 0.0004 0.011 0.0131 0.019 0.0062
Eisosomes 0.0003 0.0001 0.0001 0.0004 0.0014 0 0.0008 0.0008 0.0002 0.0014 0 0.0001 0.0002 0 0.0001 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.8469 0.9108 0.9211 0.859 0.6289 0.9493 0.2518 0.7182 0.5746 0.6508 0.9848 0.7629 0.7833 0.9559 0.8699 0.8163 0.848 0.9529
Endosome 0.0177 0.0082 0.0081 0.004 0.057 0.0065 0.0593 0.0031 0.0567 0.0392 0.0005 0.0228 0.0366 0.0007 0.0269 0.0291 0.025 0.0081
Golgi 0.0069 0.0039 0.0073 0.0029 0.0169 0.0022 0.0167 0.0027 0.0143 0.0333 0.0001 0.006 0.0262 0.0002 0.0172 0.0182 0.018 0.0044
Lipid Particles 0.0138 0.007 0.0066 0.0024 0.0322 0.0044 0.02 0.0163 0.0032 0.0041 0.0001 0.0028 0.0222 0.002 0.0059 0.0186 0.0265 0.0006
Mitochondria 0.01 0.0001 0.0055 0.001 0.0636 0.0028 0.0184 0.0001 0.0045 0.094 0 0.0126 0.0066 0 0.0145 0.0044 0.0078 0.001
None 0.0016 0.0001 0.0001 0.0008 0.0054 0.0006 0.0937 0.0886 0.0836 0.0009 0 0.1044 0.0023 0 0.0017 0.0031 0.0003 0.0016
Nuclear Periphery 0.0242 0.0054 0.006 0.0154 0.0731 0.007 0.2185 0.0153 0.0309 0.014 0.012 0.027 0.0394 0.0074 0.006 0.0137 0.0129 0.0079
Nucleolus 0.0004 0 0.0001 0.0001 0.0025 0.0002 0.0023 0.0003 0.0021 0.0009 0 0.0003 0.0004 0 0.0006 0.0002 0 0.0002
Nucleus 0.0095 0.0006 0.0004 0.0019 0.0216 0.0009 0.0492 0.0215 0.0481 0.0035 0.0003 0.0024 0.0036 0.0003 0.0008 0.0016 0.0009 0.0007
Peroxisomes 0.0056 0 0.0017 0.0003 0.0042 0.0008 0.0027 0 0.0014 0.0017 0 0.0004 0.0084 0 0.007 0.0008 0.0064 0
Punctate Nuclear 0.0033 0.0001 0.0002 0.0013 0.0104 0.001 0.069 0.0007 0.0023 0.001 0 0.0011 0.0033 0 0.0006 0.0014 0.0005 0.0052
Vacuole 0.0062 0.0084 0.0021 0.0026 0.0085 0.0018 0.0062 0.0035 0.0219 0.0037 0.0006 0.0061 0.0028 0.0015 0.0024 0.0065 0.0029 0.0015
Vacuole Periphery 0.002 0.0047 0.0004 0.001 0.0073 0.0004 0.011 0.0005 0.0039 0.0029 0.0003 0.007 0.0014 0.0001 0.0008 0.0025 0.0014 0.0011

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 101.7584 83.294 79.6994 80.6267 46.0975 85.7728 90.3132 85.2627 81.9855 84.8651
Translational Efficiency 1.2433 1.4478 1.5904 1.3051 2.0368 1.167 1.2004 1.1209 1.1678 1.0717

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description A cargo receptor protein for Pma1p; works with Psg1p to promote the transport and maturation of Pma1p; localizes to the ER and COPII vesicles; a non-essential protein
Localization
Cell Percentages ER (99%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Exp1

Exp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Exp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available