Standard name | |
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Human Ortholog | |
Description | Ribosomal 60S subunit protein L35A; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35A has a paralog, RPL35B, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | 0 | 0 | 0 | 0 | 0 | 5 | 3 | 19 | 16 | 27 | 36 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 |
Bud | – | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 2 | 3 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 5 | 8 | 4 |
Bud Neck | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | – | 0 | 2 | 6 | 14 | 26 | 34 | 28 | 25 | 41 | 45 | 52 | 0 | 2 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | – | 34 | 511 | 489 | 491 | 589 | 673 | 560 | 568 | 616 | 452 | 556 | 118 | 176 | 222 | 116 | 96 | 83 | 14 | 53 | 485 | 156 | 205 | 168 |
Endoplasmic Reticulum | – | 3 | 4 | 8 | 33 | 44 | 130 | 151 | 111 | 185 | 93 | 146 | 1 | 2 | 5 | 6 | 7 | 3 | 0 | 0 | 11 | 2 | 1 | 4 |
Endosome | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Golgi | – | 0 | 1 | 0 | 1 | 1 | 19 | 16 | 30 | 33 | 37 | 56 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | – | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 7 | 6 | 5 | 8 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 |
Nucleus | – | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 5 | 5 | 3 |
Nuclear Periphery | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 |
Nucleolus | – | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Vac/Vac Membrane | – | 0 | 8 | 3 | 5 | 2 | 7 | 9 | 5 | 4 | 2 | 1 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 2 | 3 | 4 | 4 | 2 |
Unique Cell Count | 37 | 524 | 497 | 529 | 637 | 825 | 747 | 720 | 845 | 611 | 782 | 121 | 178 | 227 | 118 | 97 | 85 | 15 | 61 | 524 | 179 | 236 | 194 | |
Labelled Cell Count | 37 | 527 | 507 | 545 | 663 | 870 | 776 | 768 | 904 | 664 | 857 | 121 | 180 | 227 | 126 | 105 | 87 | 15 | 61 | 524 | 179 | 236 | 194 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | 114.8 | 113.8 | 102.7 | 82.0 | 62.4 | 54.0 | 48.1 | 44.0 | 44.1 | 43.6 | 43.9 | 112.3 | 102.3 | 98.4 | 122.1 | 145.2 | 162.8 | 105.6 | 111.4 | 99.0 |
Std Deviation (1e-4) | – | 30.0 | 30.2 | 22.4 | 18.4 | 13.0 | 11.9 | 12.2 | 10.3 | 10.0 | 10.0 | 10.7 | 31.1 | 30.4 | 24.2 | 33.7 | 46.3 | 57.3 | 44.4 | 50.0 | 50.7 |
Intensity Change (Log2) | – | – | – | -0.15 | -0.47 | -0.87 | -1.08 | -1.24 | -1.37 | -1.37 | -1.39 | -1.37 | -0.02 | -0.15 | -0.21 | 0.1 | 0.35 | 0.52 | -0.11 | -0.03 | -0.2 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 3.7 | 3.2 | 4.9 | 5.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 3.0 | 4.1 | 4.2 | 3.9 | 3.7 | 4.6 | 5.9 | 5.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.0 | -3.6 | -3.8 | -8.7 | -10.9 | -9.6 | -11.6 | -11.0 | -12.1 | 0 | 1.1 | 0.2 | 0.5 | 0.9 | 0.1 |
Endoplasmic Reticulum | 1.3 | 4.8 | 5.2 | 9.0 | 10.4 | 8.8 | 11.0 | 8.7 | 9.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 3.1 | 3.0 | 4.4 | 4.3 | 5.5 | 6.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.4 | -0.9 | 0 | -1.2 | -0.5 | -1.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | – | – | – | – | – | – | 0.0389 | 0.0167 | 0.0044 | 0.119 | 0.0199 | 0.0149 | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | 0.013 | 0.0241 | 0.0046 | 0.0127 | 0.0103 | 0.0405 | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | 0.0016 | 0.002 | 0.0026 | 0.0013 | 0.0078 | 0.0046 | – | – | – | – | – | – |
Bud Periphery | – | – | – | – | – | – | 0.0344 | 0.0146 | 0.0037 | 0.03 | 0.0046 | 0.058 | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | 0.0084 | 0.0051 | 0.0291 | 0.0072 | 0.0024 | 0.0149 | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | 0.0636 | 0.0167 | 0.1576 | 0.0076 | 0.0161 | 0.1616 | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | 0.1932 | 0.8196 | 0.2824 | 0.0017 | 0.6476 | 0.0549 | – | – | – | – | – | – |
Cytoplasmic Foci | – | – | – | – | – | – | 0.0057 | 0.0035 | 0.0148 | 0.0012 | 0.0111 | 0.0027 | – | – | – | – | – | – |
Eisosomes | – | – | – | – | – | – | 0.0041 | 0.0001 | 0.0009 | 0.0026 | 0.0002 | 0.0006 | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | 0.0317 | 0.0035 | 0.0064 | 0.0109 | 0.0016 | 0.0018 | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | 0.0069 | 0.0057 | 0.0529 | 0.0177 | 0.0057 | 0.0062 | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | 0.0014 | 0.002 | 0.002 | 0.0185 | 0.0014 | 0.0016 | – | – | – | – | – | – |
Lipid Particles | – | – | – | – | – | – | 0.0023 | 0.0011 | 0.0314 | 0.0077 | 0.0024 | 0.0094 | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | 0.2028 | 0.0086 | 0.0093 | 0.2807 | 0.0133 | 0.0462 | – | – | – | – | – | – |
None | – | – | – | – | – | – | 0.0022 | 0.0007 | 0.0275 | 0.0031 | 0.0039 | 0.0015 | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | 0.0688 | 0.0037 | 0.0336 | 0.1817 | 0.0088 | 0.003 | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | 0.0846 | 0.0007 | 0.0218 | 0.0062 | 0.005 | 0.0166 | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | 0.0268 | 0.0048 | 0.0289 | 0.0195 | 0.0196 | 0.0154 | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | 0.0013 | 0.001 | 0.0023 | 0.0027 | 0.0024 | 0.0039 | – | – | – | – | – | – |
Punctate Nuclear | – | – | – | – | – | – | 0.0005 | 0.0001 | 0.0326 | 0.0006 | 0.0014 | 0.0001 | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | 0.1384 | 0.0533 | 0.2173 | 0.0434 | 0.1621 | 0.3712 | – | – | – | – | – | – |
Vacuole Periphery | – | – | – | – | – | – | 0.0696 | 0.0123 | 0.0338 | 0.2241 | 0.0525 | 0.1704 | – | – | – | – | – | – |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 2446.9437 | 2600.4463 | 3199.9415 | 2611.8191 | 3022.8085 | 3050.1509 | 2310.6045 | 2200.1777 | 2450.8541 | 2233.6993 |
Translational Efficiency | 1.0551 | 1.0826 | 0.9092 | 1.0448 | 1.1687 | 0.8773 | 0.9016 | 1.2296 | 0.9726 | 1.1805 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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