Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L35A; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35A has a paralog, RPL35B, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0.05 0.07 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.92 0.98 0.98 0.93 0.92 0.82 0.75 0.79 0.73 0.74 0.71 0.98 0.99 0.98 0.98 0.99 0.98 0.95 0.88 0.93 0.87 0.87 0.87
Endoplasmic Reticulum 0.08 0 0 0.06 0.07 0.16 0.2 0.15 0.22 0.15 0.19 0 0 0 0.05 0.07 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0.06 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 5 3 19 16 27 36 0 0 0 0 0 0 0 0 2 0 1 0
Bud 0 0 1 0 1 1 1 2 3 2 2 0 0 0 0 0 0 0 0 5 5 8 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 2 6 14 26 34 28 25 41 45 52 0 2 0 1 0 1 0 0 0 0 0 0
Cytoplasm 34 511 489 491 589 673 560 568 616 452 556 118 176 222 116 96 83 14 53 485 156 205 168
Endoplasmic Reticulum 3 4 8 33 44 130 151 111 185 93 146 1 2 5 6 7 3 0 0 11 2 1 4
Endosome 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Golgi 0 1 0 1 1 19 16 30 33 37 56 0 0 0 1 0 0 0 0 0 0 0 1
Mitochondria 0 0 0 0 0 1 3 7 6 5 8 0 0 0 2 0 0 0 0 2 0 1 0
Nucleus 0 0 0 1 0 0 2 0 0 0 0 1 0 0 0 0 0 0 1 0 5 5 3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0
Nucleolus 0 0 0 0 0 0 3 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Vac/Vac Membrane 0 8 3 5 2 7 9 5 4 2 1 1 0 0 0 2 0 0 2 3 4 4 2
Unique Cell Count 37 524 497 529 637 825 747 720 845 611 782 121 178 227 118 97 85 15 61 524 179 236 194
Labelled Cell Count 37 527 507 545 663 870 776 768 904 664 857 121 180 227 126 105 87 15 61 524 179 236 194


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 114.8 113.8 102.7 82.0 62.4 54.0 48.1 44.0 44.1 43.6 43.9 112.3 102.3 98.4 122.1 145.2 162.8 105.6 111.4 99.0
Std Deviation (1e-4) 30.0 30.2 22.4 18.4 13.0 11.9 12.2 10.3 10.0 10.0 10.7 31.1 30.4 24.2 33.7 46.3 57.3 44.4 50.0 50.7
Intensity Change (Log2) -0.15 -0.47 -0.87 -1.08 -1.24 -1.37 -1.37 -1.39 -1.37 -0.02 -0.15 -0.21 0.1 0.35 0.52 -0.11 -0.03 -0.2


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 3.7 3.2 4.9 5.0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 3.0 4.1 4.2 3.9 3.7 4.6 5.9 5.6 0 0 0 0 0 0
Cytoplasm 1.0 -3.6 -3.8 -8.7 -10.9 -9.6 -11.6 -11.0 -12.1 0 1.1 0.2 0.5 0.9 0.1
Endoplasmic Reticulum 1.3 4.8 5.2 9.0 10.4 8.8 11.0 8.7 9.9 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 3.1 3.0 4.4 4.3 5.5 6.0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.4 -0.9 0 -1.2 -0.5 -1.4 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin 0.0389 0.0167 0.0044 0.119 0.0199 0.0149
Bud 0.013 0.0241 0.0046 0.0127 0.0103 0.0405
Bud Neck 0.0016 0.002 0.0026 0.0013 0.0078 0.0046
Bud Periphery 0.0344 0.0146 0.0037 0.03 0.0046 0.058
Bud Site 0.0084 0.0051 0.0291 0.0072 0.0024 0.0149
Cell Periphery 0.0636 0.0167 0.1576 0.0076 0.0161 0.1616
Cytoplasm 0.1932 0.8196 0.2824 0.0017 0.6476 0.0549
Cytoplasmic Foci 0.0057 0.0035 0.0148 0.0012 0.0111 0.0027
Eisosomes 0.0041 0.0001 0.0009 0.0026 0.0002 0.0006
Endoplasmic Reticulum 0.0317 0.0035 0.0064 0.0109 0.0016 0.0018
Endosome 0.0069 0.0057 0.0529 0.0177 0.0057 0.0062
Golgi 0.0014 0.002 0.002 0.0185 0.0014 0.0016
Lipid Particles 0.0023 0.0011 0.0314 0.0077 0.0024 0.0094
Mitochondria 0.2028 0.0086 0.0093 0.2807 0.0133 0.0462
None 0.0022 0.0007 0.0275 0.0031 0.0039 0.0015
Nuclear Periphery 0.0688 0.0037 0.0336 0.1817 0.0088 0.003
Nucleolus 0.0846 0.0007 0.0218 0.0062 0.005 0.0166
Nucleus 0.0268 0.0048 0.0289 0.0195 0.0196 0.0154
Peroxisomes 0.0013 0.001 0.0023 0.0027 0.0024 0.0039
Punctate Nuclear 0.0005 0.0001 0.0326 0.0006 0.0014 0.0001
Vacuole 0.1384 0.0533 0.2173 0.0434 0.1621 0.3712
Vacuole Periphery 0.0696 0.0123 0.0338 0.2241 0.0525 0.1704

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 2446.9437 2600.4463 3199.9415 2611.8191 3022.8085 3050.1509 2310.6045 2200.1777 2450.8541 2233.6993
Translational Efficiency 1.0551 1.0826 0.9092 1.0448 1.1687 0.8773 0.9016 1.2296 0.9726 1.1805

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

No result is found for the queried gene(s). Please try another query.


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpl35a-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available