Standard name
Human Ortholog
Description Forms dehydrodolichyl diphosphate syntase complex with RER2 or SRT1; tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY, suggesting that Nus1p may be involved in protein trafficking; mutations in human homolog NUS1 have been implicated in congenital scoliosis, neurological impairment, refractory epilepsy, hearing deficit, and visual impairment; human cis-prenyltransferase complex complements yeast null mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0.21 0.14 0.18 0 0 0 0 0 0 0 – – – – – – 0 0.06 0 0 0 0.06
Bud – 0 0 0.09 0.09 0.06 0.07 0 0.08 0.05 0 0.06 – – – – – – 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 – – – – – – 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – – – – –
Cell Periphery – 0 0.06 0.05 0.05 0 0 0 0 0 0 0 – – – – – – 0 0 0 0 0 0
Cytoplasm – 0.2 0.08 0.09 0.07 0 0 0 0 0 0 0 – – – – – – 0.05 0.06 0 0.9 0.18 0.55
Endoplasmic Reticulum – 0.42 0.25 0.06 0 0 0 0 0 0 0 0 – – – – – – 0 0.59 0.45 0 0 0
Endosome – 0.05 0 0.11 0.17 0.08 0 0 0 0 0 0 – – – – – – 0.28 0.06 0.07 0.09 0 0
Golgi – 0.09 0 0 0 0 0 0 0 0 0 0 – – – – – – 0 0.08 0.07 0 0 0
Mitochondria – 0.06 0.46 0.67 0.61 0.8 0.88 0.79 0.92 0.88 0.91 0.9 – – – – – – 0.31 0 0.26 0 0 0
Nucleus – 0 0 0 0 0 0 0 0 0 0 0 – – – – – – 0 0 0 0 0.31 0
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 – – – – – – 0 0 0 0 0 0.08
Nucleolus – 0.07 0 0 0 0.06 0.09 0.08 0.07 0.06 0.1 0.09 – – – – – – 0 0 0 0 0.4 0.1
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 – – – – – – 0 0 0 0 0 0
SpindlePole – 0 0 0 0.05 0 0 0 0 0 0 0 – – – – – – 0.05 0 0 0 0.05 0
Vac/Vac Membrane – 0.25 0.06 0.16 0.11 0.12 0.12 0.19 0.1 0.15 0.16 0.19 – – – – – – 0 0.06 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 3 30 17 34 7 2 6 2 1 0 0 – – – – – – 0 6 7 0 0 0
Bud – 0 6 11 16 14 17 10 18 12 8 12 – – – – – – 0 1 0 0 0 0
Bud Neck – 0 0 0 0 0 1 0 0 0 0 0 – – – – – – 0 0 1 0 0 0
Bud Site – 0 0 0 0 2 3 5 7 2 1 1 – – – – – – – – – – – –
Cell Periphery – 0 9 6 9 1 5 1 4 2 3 4 – – – – – – 0 0 4 0 0 0
Cytoplasm – 16 12 11 14 9 10 5 4 6 7 3 – – – – – – 0 6 2 0 0 3
Endoplasmic Reticulum – 34 36 7 8 2 0 1 2 2 2 1 – – – – – – 0 61 105 0 0 0
Endosome – 4 5 14 32 18 4 4 1 0 1 0 – – – – – – 1 5 17 0 0 0
Golgi – 7 2 2 6 0 1 1 1 0 1 0 – – – – – – 0 8 17 0 0 0
Mitochondria – 5 67 84 114 176 205 195 212 221 193 188 – – – – – – 1 4 61 0 0 0
Nucleus – 0 1 0 0 1 1 5 3 3 2 1 – – – – – – 0 0 0 0 0 0
Nuclear Periphery – 3 0 0 0 2 1 3 3 6 6 6 – – – – – – 0 0 2 0 0 0
Nucleolus – 6 0 1 0 14 20 19 16 14 21 18 – – – – – – 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 – – – – – – 0 0 0 0 0 0
SpindlePole – 0 3 1 10 8 6 1 4 1 0 2 – – – – – – 0 0 0 0 0 0
Vac/Vac Membrane – 20 9 20 20 27 28 46 22 37 35 40 – – – – – – 0 5 7 0 0 0
Unique Cell Count 80 146 125 187 221 233 246 231 252 213 209 6 104 235 1 2 6
Labelled Cell Count 98 180 174 263 281 304 302 299 307 280 276 6 104 235 1 2 6


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 18.7 7.5 6.6 7.3 6.7 6.6 7.1 6.5 6.5 6.6 6.7 – – – – – – – – –
Std Deviation (1e-4) – 9.4 1.4 1.5 1.5 1.6 1.3 1.4 1.7 1.3 1.4 1.5 – – – – – – – – –
Intensity Change (Log2) – – – -0.19 -0.04 -0.18 -0.2 -0.09 -0.21 -0.21 -0.19 -0.16 – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.5 -0.5 -5.4 -6.7 -6.0 -6.7 -7.2 -6.9 -6.8 – – – – – –
Bud 1.6 1.6 0.9 1.3 0 1.4 0.3 -0.2 0.7 – – – – – –
Bud Neck 0 0 0 0 0 0 0 0 0 – – – – – –
Bud Site 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery -0.5 -0.5 0 -2.0 0 -2.3 0 0 -2.1 – – – – – –
Cytoplasm 0.2 -0.2 -1.7 -1.6 -2.9 -3.0 -2.7 -2.1 -3.1 – – – – – –
Endoplasmic Reticulum -4.3 -5.4 -7.3 -8.0 -7.9 -7.5 -7.8 -7.2 -7.3 – – – – – –
Endosome 2.5 3.9 1.8 0 0 0 0 0 0 – – – – – –
Golgi 0 0 0 0 0 0 0 0 0 – – – – – –
Mitochondria 3.5 2.7 6.7 8.9 6.8 9.9 9.0 9.3 9.1 – – – – – –
Nucleus 0 0 0 0 0 0 0 0 0 – – – – – –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – – – – – –
Nucleolus 0 0 3.1 3.6 3.4 3.2 2.9 3.9 3.6 – – – – – –
Peroxisomes 0 0 0 0 0 0 0 0 0 – – – – – –
SpindlePole 0 1.5 0 0 0 0 0 0 0 – – – – – –
Vacuole 2.6 1.5 1.9 1.9 3.5 1.2 2.6 2.9 3.5 – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0007 0.0038 0.0037 0.0055 0.0006 0.0017 0.0025 0.0057 0.0256 0.1429 0.0015 0.0046 – – – – – –
Bud 0.0006 0.0004 0.0008 0.0004 0.0001 0.0002 0.0001 0.0002 0.0004 0.0003 0 0.0001 – – – – – –
Bud Neck 0.0013 0.0007 0.0005 0.0008 0.0003 0.0011 0.0007 0.0008 0.0035 0.0019 0.0003 0.0009 – – – – – –
Bud Periphery 0.0003 0.0003 0.001 0.0008 0.0001 0.0004 0.0001 0.0004 0.0008 0.0004 0 0.0002 – – – – – –
Bud Site 0.0186 0.0086 0.0024 0.0006 0.0002 0.0004 0.0004 0.0011 0.0013 0.0022 0.0001 0.0002 – – – – – –
Cell Periphery 0.0007 0.0004 0.0005 0.0008 0.0003 0.0004 0.0006 0.0047 0.001 0.0005 0.0003 0.0025 – – – – – –
Cytoplasm 0.4121 0.1247 0.0761 0.0517 0.0885 0.0771 0.0173 0.013 0.0058 0.0022 0.0014 0.0024 – – – – – –
Cytoplasmic Foci 0.049 0.1025 0.0813 0.0807 0.0507 0.0637 0.0329 0.0579 0.0407 0.0208 0.0254 0.0379 – – – – – –
Eisosomes 0 0 0.0003 0.0005 0 0.0001 0.0002 0.0001 0.0002 0.0005 0 0.0003 – – – – – –
Endoplasmic Reticulum 0.0083 0.1485 0.1638 0.2438 0.4201 0.2504 0.0643 0.1399 0.0897 0.413 0.0056 0.1653 – – – – – –
Endosome 0.3394 0.3955 0.3757 0.3306 0.2803 0.3414 0.0512 0.0774 0.0622 0.0583 0.0271 0.051 – – – – – –
Golgi 0.0371 0.0318 0.0574 0.0418 0.0662 0.0385 0.0524 0.0718 0.0568 0.1412 0.0411 0.1535 – – – – – –
Lipid Particles 0.116 0.0757 0.0946 0.0978 0.0294 0.1169 0.6912 0.575 0.6481 0.1753 0.8908 0.4412 – – – – – –
Mitochondria 0.0019 0.0111 0.0304 0.0393 0.0065 0.0168 0.0031 0.003 0.0165 0.0032 0.0005 0.0489 – – – – – –
None 0.0017 0.012 0.0289 0.0108 0.0014 0.0106 0.0179 0.0002 0.0004 0.0004 0 0.0003 – – – – – –
Nuclear Periphery 0.0013 0.0353 0.0432 0.0496 0.0424 0.0365 0.0189 0.0106 0.015 0.0236 0.0006 0.0186 – – – – – –
Nucleolus 0.0001 0.0019 0.0007 0.0003 0 0.0006 0.0013 0.0026 0.0003 0.0001 0.0001 0.009 – – – – – –
Nucleus 0.0007 0.007 0.005 0.0037 0.0016 0.0072 0.0195 0.0013 0.0025 0.0006 0.0001 0.0021 – – – – – –
Peroxisomes 0.0013 0.0066 0.007 0.0167 0.0012 0.0043 0.0029 0.0027 0.0133 0.0052 0.0017 0.0015 – – – – – –
Punctate Nuclear 0.0004 0.0078 0.0039 0.0032 0.0007 0.0093 0.0159 0.0071 0.0019 0.0007 0.0007 0.04 – – – – – –
Vacuole 0.0066 0.0205 0.0158 0.0133 0.0066 0.0161 0.0042 0.0222 0.0098 0.0046 0.0016 0.0073 – – – – – –
Vacuole Periphery 0.0017 0.005 0.0069 0.0072 0.0029 0.0062 0.0026 0.0023 0.0043 0.0022 0.0008 0.0121 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 39.1278 37.6012 24.6573 28.342 24.0972 31.2849 24.9773 26.2567 27.8788 36.404
Translational Efficiency 1.2499 0.9597 1.2807 1.4257 1.5922 1.5963 1.2713 1.148 1.3336 1.1658

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Forms dehydrodolichyl diphosphate syntase complex with RER2 or SRT1; tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY, suggesting that Nus1p may be involved in protein trafficking; mutations in human homolog NUS1 have been implicated in congenital scoliosis, neurological impairment, refractory epilepsy, hearing deficit, and visual impairment; human cis-prenyltransferase complex complements yeast null mutant
Localization
Cell Percentages ER (92%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Nus1

Nus1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nus1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available