Standard name
Human Ortholog
Description GTPase-activating protein (GAP); contains Rho1p-specific GAP activity, interacting with activated forms of Rho1p; functions along with Sac7p as a negative regulator of the Pkc1p-mediated cell wall integrity signaling pathway; negative regulator of cell wall 1,3-beta-glucan biosynthesis; required for efficient cell fusion; contains a RhoGAP domain and three Lin-11-Isl1-Mec-3 (LIM) domains
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.58 0.46 – – – – – – – – – – – – – – – – 0.27 0.21 – – 0 –
Bud 0.13 0.21 – – – – – – – – – – – – – – – – 0.28 0.26 – – 0 –
Bud Neck 0 0 – – – – – – – – – – – – – – – – 0.24 0.29 – – 0 –
Bud Site 0.24 0.16 – – – – – – – – – – – – – – – – – – – – – –
Cell Periphery 0.07 0.05 – – – – – – – – – – – – – – – – 0.06 0 – – 0 –
Cytoplasm 0.27 0.38 – – – – – – – – – – – – – – – – 0.05 0.1 – – 0 –
Endoplasmic Reticulum 0 0.05 – – – – – – – – – – – – – – – – 0 0 – – 0 –
Endosome 0 0 – – – – – – – – – – – – – – – – 0 0 – – 0 –
Golgi 0 0 – – – – – – – – – – – – – – – – 0 0 – – 0 –
Mitochondria 0 0.09 – – – – – – – – – – – – – – – – 0 0 – – 0 –
Nucleus 0 0 – – – – – – – – – – – – – – – – 0 0 – – 0 –
Nuclear Periphery 0 0 – – – – – – – – – – – – – – – – 0 0 – – 0 –
Nucleolus 0 0 – – – – – – – – – – – – – – – – 0 0 – – 0 –
Peroxisomes 0.05 0.05 – – – – – – – – – – – – – – – – 0 0 – – 0.96 –
SpindlePole 0.12 0 – – – – – – – – – – – – – – – – 0 0 – – 0 –
Vac/Vac Membrane 0 0 – – – – – – – – – – – – – – – – 0 0 – – 0 –
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 126 78 – – – – – – – – – – – – – – – – 71 45 – – 0 –
Bud 29 35 – – – – – – – – – – – – – – – – 73 55 – – 0 –
Bud Neck 1 1 – – – – – – – – – – – – – – – – 64 62 – – 0 –
Bud Site 52 28 – – – – – – – – – – – – – – – – – – – – – –
Cell Periphery 15 8 – – – – – – – – – – – – – – – – 15 3 – – 0 –
Cytoplasm 58 64 – – – – – – – – – – – – – – – – 13 22 – – 0 –
Endoplasmic Reticulum 6 8 – – – – – – – – – – – – – – – – 4 2 – – 0 –
Endosome 1 0 – – – – – – – – – – – – – – – – 5 4 – – 0 –
Golgi 0 1 – – – – – – – – – – – – – – – – 1 2 – – 0 –
Mitochondria 4 15 – – – – – – – – – – – – – – – – 4 4 – – 0 –
Nucleus 5 1 – – – – – – – – – – – – – – – – 0 0 – – 0 –
Nuclear Periphery 3 0 – – – – – – – – – – – – – – – – 1 0 – – 0 –
Nucleolus 0 0 – – – – – – – – – – – – – – – – 0 0 – – 0 –
Peroxisomes 11 9 – – – – – – – – – – – – – – – – 1 0 – – 0 –
SpindlePole 26 4 – – – – – – – – – – – – – – – – 2 1 – – 0 –
Vac/Vac Membrane 0 1 – – – – – – – – – – – – – – – – 2 5 – – 0 –
Unique Cell Count 217 170 266 213 1
Labelled Cell Count 337 253 266 213 1


Bud Neck, Cell Periphery, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.3 4.9 – – – – – – – – – – – – – – – – – – –
Std Deviation (1e-4) 1.0 0.8 – – – – – – – – – – – – – – – – – – –
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.1144 2.088 2.4598 2.2178 1.9128 2.6825 1.1865 -0.8718 -0.3052 -0.1033 -0.4681 -0.3471 4.6312 4.2212 4.5241 5.0929 4.6966 4.8384
Actin 0.0406 0.0047 0.0739 0.0378 0.0158 0.0273 0.0916 0.0297 0.0648 0.0265 0.0308 0.0249 0.083 0.0211 0.0783 0.0158 0.0234 0.0748
Bud 0.004 0.0165 0.0549 0.0225 0.0106 0.0019 0.0087 0.0294 0.118 0.0307 0.0255 0.0049 0.0175 0.0176 0.0776 0.0254 0.0225 0.0109
Bud Neck 0.1558 0.0145 0.1202 0.2313 0.2442 0.7599 0.1107 0.0097 0.0555 0.1373 0.1323 0.7119 0.1768 0.0238 0.1717 0.1444 0.1722 0.6962
Bud Periphery 0.0125 0.0368 0.2441 0.2655 0.227 0.0088 0.0459 0.0611 0.3806 0.3205 0.179 0.0198 0.0942 0.0249 0.3226 0.238 0.2499 0.0365
Bud Site 0.2414 0.3563 0.1182 0.035 0.002 0.0073 0.1783 0.2955 0.1082 0.0449 0.0109 0.026 0.2953 0.4388 0.1697 0.02 0.0221 0.0145
Cell Periphery 0.1321 0.026 0.0399 0.0608 0.1088 0.0096 0.0246 0.0164 0.0175 0.0379 0.0682 0.0038 0.0735 0.0126 0.0244 0.1166 0.1158 0.0049
Cytoplasm 0.22 0.1717 0.1468 0.124 0.1863 0.0725 0.169 0.2553 0.0724 0.217 0.2713 0.1187 0.1315 0.1631 0.0672 0.152 0.159 0.0742
Cytoplasmic Foci 0.0048 0.0022 0.0046 0.0076 0.0026 0.0147 0.0328 0.0139 0.0124 0.0121 0.0103 0.0268 0.0158 0.0058 0.0036 0.0041 0.0031 0.0151
Eisosomes 0.0024 0.0009 0.0025 0.0012 0.0026 0.0003 0.0033 0.0008 0.0012 0.0017 0.0005 0.0003 0.0039 0.0017 0.0029 0.0009 0.0029 0.0009
Endoplasmic Reticulum 0.0075 0.0025 0.0044 0.0123 0.0082 0.0006 0.0195 0.0069 0.0077 0.0235 0.0176 0.0032 0.0083 0.0071 0.0015 0.0026 0.0047 0.0019
Endosome 0.0015 0.0002 0.0033 0.0052 0.0006 0.0004 0.0327 0.0026 0.0068 0.0073 0.0319 0.0044 0.0033 0.0008 0.0004 0.0009 0.0007 0.0009
Golgi 0.0006 0.0003 0.0016 0.0014 0.0005 0.0005 0.011 0.001 0.002 0.0041 0.0085 0.0022 0.0015 0.0006 0.0006 0.0005 0.0006 0.001
Lipid Particles 0.0012 0.0004 0.001 0.0031 0.0005 0.0003 0.0415 0.0014 0.0021 0.002 0.0081 0.0053 0.0031 0.0008 0.0004 0.0005 0.0005 0.0007
Mitochondria 0.0005 0.0003 0.0014 0.0011 0.0005 0.0002 0.0077 0.0029 0.0117 0.0086 0.0041 0.0008 0.0007 0.0008 0.0008 0.0006 0.0006 0.0005
None 0.0808 0.3616 0.1653 0.1723 0.1843 0.093 0.0693 0.2554 0.1082 0.0743 0.1695 0.0307 0.0803 0.2659 0.0716 0.2705 0.2081 0.059
Nuclear Periphery 0.0133 0.0003 0.0034 0.0011 0.0011 0.0002 0.0417 0.0014 0.0051 0.0063 0.0099 0.0006 0.001 0.0011 0.0004 0.0004 0.001 0.0004
Nucleolus 0.0007 0.0004 0.0009 0.001 0.0002 0.0002 0.0068 0.0015 0.0019 0.0044 0.0017 0.0008 0.0008 0.0017 0.0008 0.0005 0.0008 0.0003
Nucleus 0.0657 0.0011 0.0022 0.0029 0.0007 0.0005 0.0271 0.0039 0.0101 0.0155 0.005 0.0014 0.0013 0.0053 0.0016 0.0009 0.0029 0.0006
Peroxisomes 0.0005 0.0002 0.0004 0.0002 0.0001 0.0008 0.0182 0.0019 0.0008 0.0086 0.001 0.0054 0.0005 0.0004 0.0006 0.0007 0.0002 0.002
Punctate Nuclear 0.0096 0.0003 0.0007 0.001 0.0002 0.0004 0.0303 0.0021 0.0033 0.0036 0.0022 0.005 0.0014 0.0025 0.0007 0.0004 0.0022 0.0039
Vacuole 0.0042 0.0028 0.01 0.0118 0.003 0.0007 0.024 0.0064 0.0085 0.0107 0.0094 0.0025 0.0059 0.0035 0.0024 0.0041 0.0064 0.0008
Vacuole Periphery 0.0002 0.0001 0.0005 0.0008 0.0002 0.0001 0.0056 0.0007 0.0012 0.0026 0.0022 0.0002 0.0004 0.0002 0.0002 0.0002 0.0004 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.9486 8.3974 8.1486 10.3708 11.0083 8.3053 12.0828 12.7837 10.3546 14.8097
Translational Efficiency 0.5702 0.5563 0.4573 0.2968 0.4157 0.4569 0.4812 0.3473 0.4359 0.316

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description GTPase-activating protein (GAP); contains Rho1p-specific GAP activity, interacting with activated forms of Rho1p; functions along with Sac7p as a negative regulator of the Pkc1p-mediated cell wall integrity signaling pathway; negative regulator of cell wall 1,3-beta-glucan biosynthesis; required for efficient cell fusion; contains a RhoGAP domain and three Lin-11-Isl1-Mec-3 (LIM) domains
Localization
Cell Percentages bud neck (24%), cytoplasm (16%), mixed (31%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Lrg1

Lrg1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Lrg1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available