Standard name
Human Ortholog
Description Protein that localizes primarily to the plasma membrane; also found at the nuclear envelope; long-lived protein that is asymmetrically retained in the plasma membrane of mother cells; the authentic, non-tagged protein is detected in mitochondria in a phosphorylated state; null mutation confers sensitivity to acetic acid
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0.06 0.13 0.05 0 – 0 0 0 0.11 0.14 0.09 0 0 0 0 – 0 0 0 0
Bud 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
Bud Neck 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
Bud Site 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.87 – 0.95 0.87 0.81 0.81 0.72 0.61 – 0.79 0.85 0.77 0.93 0.96 0.87 0.77 0.8 0.73 0.9 – 0.96 0.79 0.8 0.84
Cytoplasm 0.07 – 0 0.09 0.08 0.11 0.08 0.12 – 0.08 0.08 0.08 0.07 0 0.06 0.12 0 0.11 0 – 0 0 0 0
Endoplasmic Reticulum 0.05 – 0 0 0.07 0.06 0.13 0.2 – 0.1 0.07 0.13 0 0 0.07 0 0.07 0 0 – 0 0 0 0
Endosome 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
Golgi 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
Mitochondria 0 – 0 0 0.08 0.07 0 0 – 0 0 0 0 0 0 0.17 0.15 0.16 0 – 0 0.1 0.1 0.05
Nucleus 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
Nuclear Periphery 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
Nucleolus 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
Vac/Vac Membrane 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 – 4 3 23 44 17 7 – 2 8 5 5 14 10 1 0 0 0 – 0 0 1 1
Bud 1 – 1 3 10 8 7 3 – 3 3 4 1 1 0 0 1 0 1 – 0 0 4 4
Bud Neck 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
Bud Site 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 84 – 117 249 300 282 242 120 – 171 222 222 41 93 93 97 37 66 103 – 130 38 101 118
Cytoplasm 7 – 3 26 28 37 27 23 – 18 22 24 3 2 6 15 2 10 1 – 1 2 3 4
Endoplasmic Reticulum 5 – 2 7 26 21 42 39 – 21 18 37 1 1 7 5 3 4 1 – 1 0 0 3
Endosome 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
Golgi 1 – 0 1 1 0 1 1 – 0 0 0 0 0 0 3 2 4 0 – 0 1 0 0
Mitochondria 1 – 3 0 30 25 15 5 – 3 1 0 0 0 0 21 7 15 2 – 2 4 12 6
Nucleus 0 – 0 0 3 2 3 1 – 0 0 1 0 0 0 0 0 0 1 – 0 0 0 0
Nuclear Periphery 0 – 1 0 0 0 0 1 – 0 1 0 0 0 0 0 0 0 0 – 0 0 0 0
Nucleolus 0 – 0 1 2 0 3 5 – 2 0 3 0 0 0 0 0 0 0 – 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
Vac/Vac Membrane 0 – 0 2 1 0 2 0 – 0 0 0 0 0 1 0 0 0 0 – 0 0 1 1
Unique Cell Count 97 123 285 372 350 335 198 217 262 287 44 97 107 126 46 91 115 137 48 128 141
Labelled Cell Count 103 131 292 424 419 359 205 220 275 296 51 111 117 142 52 99 115 137 48 128 141


Bud, Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 43.1 – 55.7 61.0 54.0 53.7 60.1 67.9 – 87.9 93.8 102.3 52.2 57.1 55.2 81.5 93.6 93.1 51.6 51.8 47.9
Std Deviation (1e-4) 12.8 – 14.5 18.4 18.4 17.3 17.7 21.7 – 25.9 28.9 29.9 16.4 18.3 19.6 27.0 26.1 28.8 13.3 18.4 18.1
Intensity Change (Log2) – – – 0.13 -0.05 -0.05 0.11 0.29 – 0.66 0.75 0.88 -0.09 0.03 -0.01 0.55 0.75 0.74 -0.11 -0.1 -0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 1.2 2.9 0.8 0 – 0 0 0 0 3.0 1.9 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery -2.4 -3.8 -3.8 -5.3 -6.8 – -4.0 -2.9 -4.4 -0.5 0.3 -2.2 -4.1 -3.0 -4.6
Cytoplasm 2.4 2.0 2.8 2.2 2.9 – 2.2 2.2 2.2 0 0 0 2.9 0 2.6
Endoplasmic Reticulum 0 2.2 1.9 3.5 4.7 – 2.8 2.2 3.6 0 0 0 0 0 0
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 0 2.2 1.9 0 0 – 0 0 0 0 0 0 3.8 0 3.7
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin – – – – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – – – – –
Bud Periphery – – – – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – – – – –
Cytoplasmic Foci – – – – – – – – – – – – – – – – – –
Eisosomes – – – – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – – – – –
Lipid Particles – – – – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – – – – –
None – – – – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – – – – –
Punctate Nuclear – – – – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – – – – –
Vacuole Periphery – – – – – – – – – – – – – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 49.2694 107.5488 689.3706 923.9888 233.2358 64.1292 220.8508 571.4235 697.9898 364.7064
Translational Efficiency 0.7332 1.0129 1.513 0.9734 1.3006 0.5926 1.2822 1.0945 1.4735 1.2038

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization –
Gene Expression ✔
Translational Efficiency ✘

No result is found for the queried gene(s). Please try another query.


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrh1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available