Standard name | |
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Human Ortholog | |
Description | Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 1 | 1 | 12 | 15 | 13 | 19 | 25 | 15 | 9 | 12 | 20 | 7 | 2 | 4 | 3 | 2 | 3 | 0 | 0 | 0 | 1 | 4 | 1 |
Bud | 0 | 0 | 0 | 1 | 0 | 1 | 4 | 8 | 7 | 6 | 9 | 7 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 0 | 4 | 7 | 3 | 13 | 11 | 14 | 11 | 13 | 10 | 5 | 7 | 13 | 9 | 12 | 8 | 0 | 0 | 0 | 1 | 0 | 0 |
Cytoplasm | 97 | 71 | 32 | 136 | 216 | 149 | 185 | 148 | 202 | 94 | 106 | 117 | 327 | 476 | 492 | 159 | 109 | 163 | 87 | 85 | 46 | 170 | 362 | 340 |
Endoplasmic Reticulum | 5 | 5 | 2 | 15 | 35 | 22 | 31 | 43 | 31 | 12 | 24 | 20 | 62 | 48 | 33 | 7 | 3 | 7 | 15 | 8 | 10 | 25 | 60 | 41 |
Endosome | 1 | 0 | 0 | 3 | 3 | 1 | 0 | 5 | 4 | 2 | 1 | 4 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 2 | 2 | 5 | 3 |
Golgi | 0 | 0 | 0 | 3 | 8 | 10 | 4 | 0 | 5 | 4 | 0 | 7 | 1 | 2 | 0 | 9 | 2 | 10 | 3 | 3 | 2 | 1 | 10 | 7 |
Mitochondria | 9 | 10 | 0 | 3 | 10 | 42 | 88 | 108 | 172 | 105 | 182 | 139 | 0 | 0 | 0 | 4 | 5 | 7 | 4 | 4 | 6 | 8 | 13 | 5 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 4 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Vac/Vac Membrane | 0 | 3 | 0 | 11 | 7 | 4 | 17 | 27 | 24 | 26 | 7 | 44 | 8 | 5 | 5 | 32 | 3 | 18 | 3 | 10 | 5 | 8 | 11 | 9 |
Unique Cell Count | 103 | 83 | 34 | 172 | 271 | 217 | 299 | 303 | 384 | 226 | 290 | 304 | 383 | 520 | 525 | 203 | 125 | 194 | 120 | 115 | 76 | 223 | 478 | 419 |
Labelled Cell Count | 113 | 92 | 35 | 189 | 301 | 245 | 363 | 375 | 474 | 271 | 355 | 371 | 410 | 542 | 552 | 227 | 136 | 217 | 120 | 115 | 76 | 223 | 478 | 419 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.0 | 9.3 | 10.6 | 11.0 | 10.3 | 9.0 | 8.4 | 8.6 | 8.0 | 7.6 | 7.1 | 7.4 | 12.6 | 13.1 | 12.9 | 14.1 | 15.3 | 15.7 | 11.7 | 12.2 | 12.5 |
Std Deviation (1e-4) | 1.5 | 2.8 | 1.4 | 1.7 | 1.5 | 1.6 | 1.7 | 1.7 | 1.7 | 1.5 | 1.4 | 1.5 | 1.7 | 2.0 | 2.0 | 3.1 | 3.2 | 2.8 | 1.8 | 1.9 | 1.9 |
Intensity Change (Log2) | – | – | – | 0.05 | -0.05 | -0.24 | -0.34 | -0.31 | -0.42 | -0.48 | -0.59 | -0.52 | 0.24 | 0.3 | 0.28 | 0.4 | 0.52 | 0.56 | 0.14 | 0.2 | 0.23 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -2.1 | -2.0 | -3.1 | -3.7 | -5.0 | -4.7 | -5.7 | -6.4 | -6.2 | -1.4 | -0.5 | -0.1 | -2.2 | -1.1 | -1.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.6 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 2.8 | 3.7 | 4.2 | 5.1 | 5.1 | 7.0 | 5.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.1661 | 6.7768 | 5.6104 | 5.1447 | 5.0012 | 5.3822 | 3.9353 | 7.2797 | 6.2894 | 4.873 | 4.4356 | 6.3865 | 4.5841 | 7.0579 | 6.0253 | 5.6797 | 5.7039 | 6.3932 |
Actin | 0.0092 | 0.0058 | 0.0169 | 0.0321 | 0.0019 | 0.0116 | 0.0126 | 0.0102 | 0.0063 | 0.0015 | 0.0061 | 0.0031 | 0.0229 | 0.0012 | 0.0099 | 0.0022 | 0.0006 | 0.0064 |
Bud | 0.0012 | 0.0009 | 0.0029 | 0.0072 | 0.0009 | 0.0007 | 0.0009 | 0.001 | 0.0013 | 0.0005 | 0.0004 | 0.0009 | 0.0012 | 0.0029 | 0.0011 | 0.0018 | 0.0005 | 0.001 |
Bud Neck | 0.0008 | 0.0004 | 0.0052 | 0.0012 | 0.0006 | 0.001 | 0.0006 | 0.0004 | 0.0006 | 0.0005 | 0.0006 | 0.0011 | 0.0018 | 0.0004 | 0.0005 | 0.0008 | 0.0005 | 0.0016 |
Bud Periphery | 0.0008 | 0.0003 | 0.0015 | 0.0113 | 0.0011 | 0.0008 | 0.0005 | 0.0003 | 0.0005 | 0.0003 | 0.0002 | 0.0007 | 0.0011 | 0.001 | 0.0005 | 0.002 | 0.0002 | 0.0009 |
Bud Site | 0.0027 | 0.0024 | 0.0204 | 0.0025 | 0.0012 | 0.0004 | 0.0029 | 0.0038 | 0.0041 | 0.0006 | 0.0004 | 0.0004 | 0.0041 | 0.0052 | 0.0018 | 0.0006 | 0.0003 | 0.0003 |
Cell Periphery | 0.0007 | 0.0002 | 0.0004 | 0.0004 | 0.0002 | 0.0003 | 0.0007 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0.0002 | 0.0005 | 0 | 0.0003 |
Cytoplasm | 0.4294 | 0.6869 | 0.4796 | 0.3782 | 0.2648 | 0.4834 | 0.5303 | 0.7135 | 0.6033 | 0.3162 | 0.4666 | 0.5484 | 0.3881 | 0.6888 | 0.4798 | 0.3712 | 0.4785 | 0.4301 |
Cytoplasmic Foci | 0.0728 | 0.0567 | 0.0635 | 0.0589 | 0.0642 | 0.0713 | 0.0843 | 0.0559 | 0.0605 | 0.0531 | 0.0567 | 0.0543 | 0.0885 | 0.06 | 0.0673 | 0.0598 | 0.0487 | 0.0644 |
Eisosomes | 0.0004 | 0 | 0.0004 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0202 | 0.0064 | 0.0131 | 0.0319 | 0.0427 | 0.0177 | 0.0222 | 0.0094 | 0.0071 | 0.0027 | 0.0038 | 0.0201 | 0.0499 | 0.0062 | 0.0097 | 0.0216 | 0.0073 | 0.0244 |
Endosome | 0.2842 | 0.1848 | 0.2542 | 0.3094 | 0.481 | 0.2925 | 0.2205 | 0.1539 | 0.2317 | 0.4829 | 0.3153 | 0.2363 | 0.2968 | 0.1807 | 0.3275 | 0.3845 | 0.3779 | 0.3239 |
Golgi | 0.0782 | 0.0257 | 0.0584 | 0.0327 | 0.0444 | 0.0444 | 0.0533 | 0.0311 | 0.046 | 0.0446 | 0.0899 | 0.063 | 0.0636 | 0.0347 | 0.0495 | 0.0552 | 0.0463 | 0.0999 |
Lipid Particles | 0.0112 | 0.0002 | 0.0055 | 0.0145 | 0.0048 | 0.0056 | 0.0144 | 0.0003 | 0.0031 | 0.0248 | 0.0123 | 0.0126 | 0.007 | 0.0002 | 0.004 | 0.0004 | 0.009 | 0.0005 |
Mitochondria | 0.039 | 0.0098 | 0.0293 | 0.009 | 0.0228 | 0.0266 | 0.0132 | 0.0068 | 0.0135 | 0.0128 | 0.022 | 0.04 | 0.0245 | 0.0066 | 0.0139 | 0.0408 | 0.0067 | 0.0292 |
None | 0.0041 | 0.0017 | 0.0205 | 0.0026 | 0.0008 | 0.0103 | 0.0043 | 0.0018 | 0.0014 | 0.0007 | 0.0007 | 0.0008 | 0.0037 | 0.0015 | 0.0009 | 0.0007 | 0.0004 | 0.0014 |
Nuclear Periphery | 0.0069 | 0.001 | 0.0014 | 0.0082 | 0.0144 | 0.0102 | 0.0066 | 0.0012 | 0.001 | 0.0018 | 0.0017 | 0.0006 | 0.0139 | 0.0006 | 0.0025 | 0.0027 | 0.0017 | 0.0015 |
Nucleolus | 0.0005 | 0 | 0.0005 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0.0002 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 |
Nucleus | 0.0057 | 0.0009 | 0.0009 | 0.0017 | 0.0023 | 0.0027 | 0.0022 | 0.001 | 0.0006 | 0.001 | 0.0006 | 0.0004 | 0.0018 | 0.0006 | 0.0008 | 0.0009 | 0.0007 | 0.0006 |
Peroxisomes | 0.0075 | 0.0003 | 0.0042 | 0.0186 | 0.001 | 0.0023 | 0.0123 | 0.0004 | 0.002 | 0.0078 | 0.0013 | 0.0052 | 0.0055 | 0.0003 | 0.0017 | 0.0003 | 0.0006 | 0.0004 |
Punctate Nuclear | 0.0021 | 0.0002 | 0.0008 | 0.0028 | 0.001 | 0.002 | 0.0016 | 0.0008 | 0.0002 | 0.0011 | 0.0003 | 0.0006 | 0.0046 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0.0006 |
Vacuole | 0.015 | 0.0109 | 0.0129 | 0.0611 | 0.0303 | 0.0096 | 0.0119 | 0.0051 | 0.0104 | 0.0278 | 0.0138 | 0.0058 | 0.014 | 0.0057 | 0.0181 | 0.0337 | 0.0109 | 0.0065 |
Vacuole Periphery | 0.0072 | 0.0044 | 0.0074 | 0.0154 | 0.0194 | 0.0062 | 0.0044 | 0.0028 | 0.0061 | 0.0192 | 0.0073 | 0.0051 | 0.0062 | 0.0033 | 0.0096 | 0.0202 | 0.009 | 0.0062 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 17.1483 | 28.5446 | 20.3969 | 22.0469 | 22.2335 | 29.5497 | 23.5167 | 23.0869 | 28.9981 | 22.2578 |
Translational Efficiency | 2.0967 | 1.4336 | 1.6589 | 2.2264 | 1.6317 | 1.3465 | 1.8258 | 1.6258 | 1.4141 | 1.7689 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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