Standard name
Human Ortholog
Description Essential Fe-S protein; required for ribosome biogenesis, translation initiation/termination; facilitates binding of multifactor complex (MFC) of initiation factors to small ribosomal subunit; Dom34-Hbs1 complex and Rli1p work in dissociating inactive ribosomes, thereby facilitating translation restart; forms complex with Lto1p and Yae1p; dependency on ROS-labile FeS clusters, activity in nuclear ribosomal-subunit export impaired by mild oxidative stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0.05 – 0 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0.06 0 – 0.08 0.12 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.97 0.96 0.97 0.92 0.9 0.84 0.75 – 0.69 0.61 0.63 0.96 0.98 0.97 0.97 0.92 0.98 0.94 0.95 0.89 0.83 0.83 0.85
Endoplasmic Reticulum 0 0 0 0 0.06 0.07 0.08 0.11 – 0.11 0.09 0.09 0 0 0 0 0 0 0 0 0.05 0.06 0.11 0.07
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.05 0.11 – 0.2 0.3 0.29 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 6 5 22 – 9 18 17 0 0 0 0 0 0 0 0 2 0 0 0
Bud 0 1 1 0 0 0 1 6 – 1 2 1 0 2 0 0 2 0 0 1 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 7 2 9 11 19 17 – 24 39 35 4 8 11 3 2 2 0 0 3 0 0 0
Cytoplasm 18 295 347 269 220 296 274 325 – 216 192 185 279 304 344 434 366 265 37 281 318 62 108 113
Endoplasmic Reticulum 0 8 9 5 14 23 26 46 – 36 29 26 10 6 13 13 6 8 0 2 18 4 14 9
Endosome 0 0 0 1 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 1 0 1 2 7 5 – 5 3 16 0 0 0 0 2 0 0 0 0 0 0 1
Mitochondria 0 0 4 1 1 12 15 48 – 63 95 84 0 0 0 6 3 2 1 0 1 3 0 0
Nucleus 0 1 0 1 2 0 2 1 – 1 0 1 0 2 2 3 2 2 0 3 0 1 0 0
Nuclear Periphery 0 0 0 0 0 0 2 0 – 0 3 2 0 0 0 0 0 0 0 0 1 1 0 0
Nucleolus 0 0 0 0 0 0 0 1 – 1 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 4 2 4 1 2 15 – 10 4 6 4 1 1 14 33 3 0 3 3 0 1 2
Unique Cell Count 18 305 360 276 238 329 327 436 315 316 293 290 310 353 446 400 270 40 298 357 76 131 133
Labelled Cell Count 18 307 373 281 251 351 353 486 366 385 375 297 323 371 473 416 282 40 298 357 76 131 133


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 48.2 38.7 33.0 28.2 24.6 21.3 19.5 16.1 – 15.3 15.1 15.9 36.6 33.5 33.8 24.9 26.3 32.9 41.3 43.0 43.5
Std Deviation (1e-4) 7.1 9.6 8.3 6.4 6.3 4.3 4.3 4.0 – 3.6 3.9 3.7 8.1 7.4 6.5 7.0 8.2 9.8 11.1 10.6 10.6
Intensity Change (Log2) – – – -0.23 -0.42 -0.63 -0.76 -1.03 – -1.11 -1.12 -1.05 0.15 0.02 0.04 -0.41 -0.33 -0.0 0.33 0.38 0.4


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 4.3 – 0 4.6 4.6 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 1.4 1.1 2.7 1.6 – 3.5 5.4 5.2 0 0.6 1.0 0 0 0
Cytoplasm 0.8 -2.1 -3.4 -5.6 -8.5 – -9.7 -11.5 -10.9 -0.1 1.3 0.8 0.7 -2.8 1.3
Endoplasmic Reticulum -0.6 2.1 2.8 3.2 4.5 – 4.6 3.8 3.6 0.7 -0.5 0.9 0.4 -1.0 0.4
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 4.1 0 0 0 0 0 0
Mitochondria 0 0 2.2 2.8 5.6 – 8.2 10.6 10.3 0 0 0 0 0 0
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 2.1 – 1.9 0 0 0 0 0 1.9 4.6 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 41.4922 48.7452 45.4735 44.7756 43.2955 44.4429 40.1363 49.2993 44.5024 44.2257 44.3258 43.2845 26.3374 48.6234 40.6787 45.4789 41.5665 43.619
Actin 0.0002 0.0002 0.0002 0.0001 0.0003 0.0002 0.0006 0.0003 0.0005 0.0002 0.0001 0.0009 0.0043 0.0004 0.0146 0.0001 0.0001 0.0005
Bud 0.0002 0.0003 0.0003 0.0004 0.0003 0.0001 0.0003 0.0002 0.0003 0.0005 0.0001 0.0003 0.0002 0.0001 0.0027 0.0005 0.0003 0.0005
Bud Neck 0.0004 0.0006 0.0006 0.0006 0.0007 0.0014 0.0004 0.0002 0.0003 0.0006 0.0003 0.0013 0.0004 0.0002 0.0004 0.0005 0.0004 0.0009
Bud Periphery 0.0006 0.0002 0.0004 0.0002 0.0002 0.0003 0.0002 0.0001 0.0001 0.0002 0 0.0002 0.0001 0 0.0009 0.0001 0 0.0002
Bud Site 0.0001 0.0005 0.0002 0.0001 0.0001 0 0.0002 0.0001 0.0002 0.0002 0 0.0001 0.0004 0.0002 0.0008 0.0001 0 0.0001
Cell Periphery 0.0059 0.0077 0.0047 0.0018 0.0009 0.0038 0.0016 0.0003 0.0003 0.0002 0.0001 0.0002 0.0003 0.0002 0.0005 0.0003 0.0001 0.0002
Cytoplasm 0.8541 0.9212 0.8994 0.905 0.9285 0.8339 0.8636 0.9783 0.9501 0.9205 0.9618 0.9266 0.6631 0.9915 0.8914 0.8732 0.8946 0.9367
Cytoplasmic Foci 0.0013 0.0011 0.0016 0.0015 0.0035 0.0023 0.0017 0.0009 0.0029 0.0038 0.0017 0.0037 0.0231 0.0005 0.0049 0.0027 0.0024 0.0039
Eisosomes 0 0 0 0 0 0 0 0 0 0 0 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0872 0.0399 0.0417 0.0072 0.0314 0.131 0.079 0.012 0.0235 0.0091 0.0059 0.0439 0.1415 0.0022 0.0229 0.0201 0.0734 0.0241
Endosome 0.0058 0.0019 0.0043 0.0065 0.0069 0.0051 0.0073 0.0014 0.0063 0.0145 0.0031 0.009 0.0862 0.0004 0.0136 0.0101 0.0089 0.0115
Golgi 0.0005 0.0007 0.0005 0.0003 0.0005 0.0008 0.0008 0.0002 0.002 0.0009 0.0001 0.002 0.0063 0.0001 0.009 0.0002 0.0002 0.0007
Lipid Particles 0.0011 0.003 0.0008 0.0001 0.0003 0.001 0.0004 0 0.0001 0 0 0.0001 0.0153 0 0.0003 0 0 0.0001
Mitochondria 0.0009 0.0003 0.0003 0.0005 0.0002 0.0024 0.0008 0.0001 0.0003 0.0004 0.0001 0.0026 0.0026 0 0.0024 0.0001 0.0006 0.0004
None 0.0004 0.0003 0.0002 0.0002 0.0002 0.0003 0.0004 0.0001 0.0002 0.0001 0.0001 0.0002 0.0043 0.0001 0.0003 0.0002 0.0004 0.0002
Nuclear Periphery 0.0047 0.0018 0.0029 0.003 0.0015 0.0032 0.0116 0.0006 0.0017 0.0045 0.002 0.0019 0.0219 0.0017 0.0087 0.0077 0.002 0.0045
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0.0004 0 0 0 0 0
Nucleus 0.0017 0.0012 0.0015 0.0026 0.0013 0.0012 0.0033 0.0006 0.0009 0.002 0.0012 0.0011 0.0054 0.0009 0.0022 0.0022 0.0012 0.0015
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0.0008 0 0.0001 0 0 0
Punctate Nuclear 0 0 0 0 0 0 0.0001 0 0 0 0 0 0.0038 0 0.0001 0 0 0.0001
Vacuole 0.0326 0.0181 0.0379 0.0655 0.0211 0.0117 0.0237 0.0041 0.0088 0.0359 0.0194 0.0045 0.0154 0.0011 0.0204 0.0705 0.0123 0.0112
Vacuole Periphery 0.0022 0.0007 0.0024 0.0045 0.0021 0.0011 0.004 0.0004 0.0015 0.0063 0.0038 0.0012 0.0043 0.0001 0.004 0.0114 0.003 0.0026

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 146.7582 134.9721 114.1768 107.5956 112.3661 98.9919 95.3926 81.6078 70.2925 106.6007
Translational Efficiency 1.111 1.1734 1.1621 1.1352 1.1314 1.0844 1.1932 1.0278 1.1299 1.0637

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

No result is found for the queried gene(s). Please try another query.


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rli1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available