Standard name
Human Ortholog
Description Subunit of SEACAT, a subcomplex of the SEA complex; Mtc1p, along with Rtc1p and Sea4p, redundantly inhibit the TORC1 inhibitory role of the Iml1p/SEACIT (Iml1p-Npr2p-Npr3p) subcomplex, a GAP for GTPase Gtr1p (EGOC subunit) in response to amino acid limitation, thereby resulting in activation of TORC1 signaling; SEA is a coatomer-related complex that associates dynamically with the vacuole; relative distribution to the vacuolar membrane decreases upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.07 0.16 0.2 0.39 0.48 0.57 0.68 0.62 0.73 – 0.7 0.63 0.23 0.26 0.37 0.33 0.46 0.34 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0.06 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0.28 0.05 0 0 0.1 0 0 0 0 – 0 0 0.06 0.09 0.06 0.12 0.12 0.12 0.34 0.12 0.16 0.11 0.17 0.25
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.22 0.23 0.64 0.63 0.38 0.6 0.52 0.56 0.58 – 0.65 0.73 0.06 0.06 0.18 0.07 0.1 0.07 0.05 0 0.08 0 0 0
Nucleus 0.06 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.05 0.07 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0
Nucleolus 0.11 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.07 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.58 0.7 0.39 0.33 0.39 0.18 0.12 0.05 0.05 – 0 0 0.76 0.7 0.58 0.59 0.52 0.59 0.48 0.72 0.63 0.74 0.61 0.59
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 0 0 1
Bud 0 0 1 2 4 3 9 16 13 – 13 10 1 2 2 0 0 0 0 1 0 1 4 3
Bud Neck 0 0 0 0 0 0 1 1 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 1 0 1 1 3 – 1 4 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 17 20 25 62 174 196 377 447 295 – 230 290 39 74 111 80 47 62 0 2 4 5 7 7
Endoplasmic Reticulum 4 4 7 6 0 4 0 8 0 – 1 1 0 7 7 13 4 6 0 0 2 1 1 2
Endosome 64 7 1 1 35 0 1 4 1 – 0 1 10 27 17 29 12 23 86 17 22 15 38 76
Golgi 0 0 0 0 0 0 0 2 0 – 0 0 0 0 0 2 0 2 0 0 0 0 0 2
Mitochondria 49 30 79 101 136 204 290 408 232 – 213 337 10 17 54 17 10 12 11 6 10 1 6 4
Nucleus 13 1 5 1 3 5 1 4 1 – 0 2 3 6 3 7 0 6 2 2 1 1 4 7
Nuclear Periphery 12 9 0 2 0 0 1 3 0 – 0 0 1 3 2 0 1 2 14 3 3 1 7 9
Nucleolus 26 4 1 3 0 0 1 0 0 – 0 0 2 2 0 1 1 1 3 1 0 0 5 1
Peroxisomes 1 0 0 0 1 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 15 3 0 1 3 1 1 3 2 – 3 0 0 0 0 2 0 2 6 1 0 1 6 4
Vac/Vac Membrane 131 89 48 53 142 63 67 39 21 – 12 19 129 201 176 141 53 109 121 104 83 98 140 175
Unique Cell Count 227 128 123 160 360 342 558 726 402 330 460 169 286 303 241 102 184 252 146 134 132 230 300
Labelled Cell Count 332 167 167 232 499 476 750 936 568 473 665 195 339 372 292 128 225 252 146 134 132 230 300


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.7 4.8 3.8 3.8 4.3 3.7 3.6 3.6 3.4 – 3.3 3.0 5.7 5.6 5.0 6.4 6.6 7.0 5.3 5.4 5.8
Std Deviation (1e-4) 0.8 0.9 0.7 0.8 1.4 1.0 1.2 0.9 1.3 – 1.1 0.7 1.4 1.1 1.3 1.5 1.6 1.7 1.2 1.5 1.4
Intensity Change (Log2) – – – -0.01 0.18 -0.03 -0.08 -0.09 -0.17 – -0.2 -0.36 0.57 0.56 0.38 0.74 0.78 0.87 0.46 0.5 0.6


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 3.5 1.9 3.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.503 3.4965 3.48 2.9923 2.5802 3.1839 1.6879 2.2403 1.9198 2.2192 1.6093 1.8419 -0.1796 0.3639 -0.1637 -0.3282 -0.2494 -0.129
Actin 0.0001 0 0.0001 0.0001 0 0.001 0.0405 0.0001 0.0128 0.0425 0.0037 0.0017 0.0513 0.0259 0.0087 0.0001 0.0018 0.0011
Bud 0.0003 0.0007 0.0008 0.0008 0.0003 0.0006 0.0008 0.0005 0.0009 0.0028 0.0002 0.0007 0.0005 0.0006 0.0004 0.0034 0.0004 0.0002
Bud Neck 0.0004 0.0006 0.0053 0.0004 0.0004 0.0009 0.0013 0.0005 0.0062 0.0021 0.0003 0.0009 0.0019 0.0008 0.0003 0.0007 0.0003 0.0006
Bud Periphery 0.0005 0.0018 0.0016 0.0018 0.0007 0.0013 0.0011 0.0005 0.0011 0.0032 0.0004 0.0009 0.0006 0.0007 0.0005 0.0085 0.0012 0.0002
Bud Site 0.0009 0.0022 0.0016 0.0006 0.0006 0.0004 0.0038 0.0011 0.0023 0.0024 0.0004 0.0008 0.0025 0.0039 0.0014 0.0015 0.0005 0.0002
Cell Periphery 0.0015 0.0007 0.0009 0.001 0.0015 0.0005 0.0004 0.0003 0.0003 0.0003 0.0001 0.0001 0.0005 0.0005 0.0004 0.0005 0.0002 0.0001
Cytoplasm 0.1131 0.1006 0.1216 0.03 0.0765 0.1278 0.0668 0.1077 0.0823 0.0515 0.044 0.0562 0.0552 0.0938 0.0859 0.0403 0.0576 0.08
Cytoplasmic Foci 0.0363 0.0584 0.0508 0.0442 0.031 0.0444 0.0364 0.0236 0.0315 0.041 0.0358 0.024 0.0743 0.0393 0.06 0.0222 0.0594 0.0513
Eisosomes 0 0 0 0.0001 0 0 0.0003 0 0.0002 0.0002 0.0002 0 0.0003 0.0002 0.0002 0 0.0002 0
Endoplasmic Reticulum 0.012 0.0114 0.0123 0.009 0.016 0.0084 0.0064 0.0075 0.0089 0.003 0.0061 0.0036 0.007 0.0027 0.0101 0.0025 0.002 0.0038
Endosome 0.3686 0.3834 0.41 0.4543 0.5013 0.3878 0.3257 0.2811 0.4357 0.3516 0.4375 0.3729 0.3107 0.3685 0.3831 0.471 0.4033 0.4666
Golgi 0.0059 0.0063 0.0094 0.0124 0.008 0.0071 0.0175 0.0042 0.0093 0.0327 0.0177 0.0083 0.0095 0.0079 0.0096 0.0042 0.0144 0.0079
Lipid Particles 0.017 0.0146 0.0088 0.0052 0.0041 0.0073 0.0209 0.0019 0.0055 0.0238 0.013 0.0029 0.0208 0.0159 0.0195 0.0095 0.0549 0.0209
Mitochondria 0.0071 0.0092 0.0263 0.0184 0.0275 0.0149 0.019 0.0085 0.0119 0.0363 0.015 0.0075 0.0054 0.0117 0.0052 0.0076 0.0451 0.004
None 0.0764 0.0266 0.0565 0.0308 0.015 0.0346 0.06 0.0209 0.0055 0.0012 0.0272 0.0075 0.0578 0.1685 0.149 0.0834 0.1263 0.0694
Nuclear Periphery 0.015 0.0115 0.0063 0.0047 0.0066 0.0099 0.0154 0.0136 0.0131 0.0094 0.0189 0.0095 0.0127 0.0033 0.0149 0.0035 0.0024 0.0063
Nucleolus 0.001 0.0015 0.0016 0.0008 0.0003 0.0007 0.0018 0.0015 0.0008 0.0008 0.0006 0.0005 0.0024 0.0017 0.0014 0.0008 0.0008 0.0007
Nucleus 0.0032 0.004 0.0035 0.0027 0.0021 0.0039 0.0106 0.0054 0.0041 0.0036 0.0016 0.0044 0.0031 0.0033 0.0067 0.003 0.0022 0.0031
Peroxisomes 0.0006 0.0018 0.0017 0.0067 0.0003 0.0009 0.0104 0.0004 0.0011 0.0121 0.0015 0.0009 0.0307 0.0023 0.0059 0.0013 0.0252 0.0018
Punctate Nuclear 0.0009 0.0011 0.0007 0.0003 0.0003 0.0006 0.0022 0.0003 0.0005 0.0034 0.001 0.0003 0.0061 0.0007 0.003 0.0006 0.0011 0.0026
Vacuole 0.2618 0.289 0.2142 0.2878 0.2222 0.2289 0.2892 0.395 0.2791 0.2933 0.2767 0.3408 0.2668 0.1883 0.1782 0.2767 0.1633 0.2115
Vacuole Periphery 0.0775 0.0744 0.066 0.0881 0.0851 0.118 0.0694 0.1253 0.0869 0.0828 0.0978 0.1555 0.0798 0.0598 0.0557 0.0586 0.0376 0.0679

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 17.1257 23.4647 21.2339 25.2645 15.3561 17.2761 24.3038 25.6792 24.5151 24.5065
Translational Efficiency 0.3475 0.2449 0.2967 0.2176 0.3116 0.341 0.2244 0.1878 0.2214 0.1869

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of SEACAT, a subcomplex of the SEA complex; Mtc1p, along with Rtc1p and Sea4p, redundantly inhibit the TORC1 inhibitory role of the Iml1p/SEACIT (Iml1p-Npr2p-Npr3p) subcomplex, a GAP for GTPase Gtr1p (EGOC subunit) in response to amino acid limitation, thereby resulting in activation of TORC1 signaling; SEA is a coatomer-related complex that associates dynamically with the vacuole; relative distribution to the vacuolar membrane decreases upon DNA replication stress
Localization
Cell Percentages vacuolar membrane (32%)
Cell Cycle Regulation No
Subcompartmental Group vac memb-1

Mtc5

Mtc5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mtc5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available