Standard name
Human Ortholog
Description Ribosomal RNA processing element (RRPE)-binding protein; involved in the glucose-induced transition from quiescence to growth; restricted to nucleus in quiescent cells, released into cytoplasm after glucose repletion; binds Sin3p; relative distribution to the nucleus increases upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0.08 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.76 0.88 0.7 0.08 0.07 0.06 0.07 0.07 0 0 0 0 0.17 0.16 0.18 0.93 0.93 0.93 0.57 0.82 0.58 0.59 0.85 0.79
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0.06 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0 0.05 0.05 0.31 0.27 0.22 0.39 0.38 0.35 0.44 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.09 0 0.11 0.86 0.82 0.71 0.64 0.67 0.68 0.62 0.64 0.59 0.65 0.73 0.73 0 0.05 0 0.1 0.05 0.12 0.11 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0.19 0.13 0.21 0.23 0.27 0.32 0.36 0.36 0.34 0.06 0.09 0.09 0 0 0 0.09 0 0.05 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0.05 0 0
Vac/Vac Membrane 0.1 0.08 0.13 0 0 0 0 0 0 0 0 0 0.12 0.05 0 0 0.07 0.05 0 0 0.06 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 1 0 0 2
Bud 0 1 4 0 0 1 0 1 2 0 2 3 1 0 1 0 0 0 5 3 11 9 5 4
Bud Neck 0 0 0 1 0 0 1 0 1 3 2 1 0 0 0 0 0 0 0 0 0 0 1 2
Bud Site 0 0 0 0 0 4 1 4 9 8 10 12 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 9 1 0 0 0 0 0 0 0 0 0 1 0 0 17 11 7 0 0 0 0 0 0
Cytoplasm 80 338 157 12 17 19 27 35 9 16 9 10 39 56 67 400 240 151 65 333 151 71 252 206
Endoplasmic Reticulum 0 6 2 0 0 0 0 0 0 0 0 0 1 0 0 12 16 7 0 1 3 0 0 4
Endosome 2 5 13 1 2 2 1 3 0 0 0 0 10 9 12 2 0 2 6 8 9 7 4 3
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 2 0 0 0
Mitochondria 5 1 8 7 11 107 101 114 117 150 106 147 4 1 1 10 0 1 0 2 1 0 1 5
Nucleus 9 12 24 129 190 246 238 354 204 245 193 196 150 250 267 14 13 4 11 19 31 13 7 6
Nuclear Periphery 0 1 0 1 0 1 3 5 2 2 9 7 0 2 1 0 0 0 0 1 1 0 0 0
Nucleolus 3 0 2 29 30 73 84 144 96 144 108 113 13 32 33 0 0 0 10 4 13 4 3 5
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 1 2 6 0 0 0 0 1 0 0 0 1
SpindlePole 0 0 0 0 0 0 0 1 1 0 2 0 3 0 5 1 0 0 9 7 10 5 7 3
Vac/Vac Membrane 10 32 29 2 6 11 4 7 1 5 3 3 27 18 9 15 17 8 1 10 14 3 5 10
Unique Cell Count 105 384 225 150 231 345 369 528 301 395 300 331 232 341 367 428 258 162 114 404 261 120 296 260
Labelled Cell Count 109 406 241 182 256 464 460 668 442 573 444 492 250 370 402 472 297 180 114 404 261 120 296 260


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.2 7.3 6.6 7.1 6.8 5.9 5.6 6.1 5.6 5.3 5.6 5.4 7.9 7.8 7.4 8.8 9.9 9.8 7.4 7.0 8.0
Std Deviation (1e-4) 0.7 0.9 1.1 2.5 1.7 1.7 1.5 1.6 1.4 1.5 1.5 1.5 1.5 1.7 1.9 1.5 1.6 1.8 1.6 1.1 1.3
Intensity Change (Log2) – – – 0.11 0.04 -0.17 -0.23 -0.12 -0.23 -0.31 -0.24 -0.3 0.26 0.25 0.17 0.41 0.59 0.58 0.16 0.08 0.28


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 2.6 2.7 0
Cytoplasm -11.8 -13.7 -16.2 -16.0 -18.2 -16.3 -17.5 -16.3 -16.9 -11.4 -12.8 -12.5 8.1 6.7 5.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 3.1 0
Endosome -2.6 -2.9 -3.8 -4.3 0 -4.2 0 -4.2 -4.4 -0.7 -1.9 -1.5 -4.3 -3.9 -2.3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.5 0.6 8.0 7.3 6.1 9.4 9.5 8.7 10.5 -1.2 0 0 -0.9 0 0
Nucleus 14.5 15.3 14.2 12.8 14.2 13.1 12.4 12.4 11.5 11.9 14.6 14.7 -3.8 -2.3 -3.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 6.4 5.1 7.0 7.3 8.4 9.0 10.0 9.8 9.5 2.8 4.2 4.1 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -4.0 -4.1 -4.4 -6.1 -6.8 -6.1 -6.1 -5.6 -6.0 -0.4 -3.2 -5.0 -4.5 -2.4 -2.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.3678 3.5678 2.8588 2.9833 2.0452 2.7668 5.4454 5.9954 5.6445 4.9102 5.2685 5.6238 5.4565 6.5504 5.9371 5.7247 5.835 5.8685
Actin 0.0406 0.0008 0.02 0.0016 0.005 0.0052 0.0095 0.0034 0.0054 0.0437 0.0067 0.0042 0.0778 0.0016 0.0323 0.0103 0.0634 0.0077
Bud 0.0003 0.0002 0.0004 0.0023 0.001 0.0054 0.0047 0.0059 0.0042 0.0033 0.0026 0.0051 0.0033 0.0059 0.0053 0.0065 0.0029 0.0046
Bud Neck 0.0014 0.0004 0.0014 0.0012 0.0039 0.0102 0.0053 0.0025 0.0022 0.0049 0.0038 0.0104 0.0022 0.0033 0.0034 0.023 0.0023 0.0058
Bud Periphery 0.0004 0.0001 0.0004 0.0019 0.0014 0.0062 0.0053 0.0028 0.0031 0.0081 0.0023 0.0053 0.0046 0.0036 0.0052 0.013 0.0017 0.0063
Bud Site 0.0028 0.0015 0.0009 0.0013 0.0348 0.0029 0.0157 0.0384 0.0105 0.0051 0.0028 0.0028 0.0084 0.0173 0.0188 0.0043 0.0015 0.0028
Cell Periphery 0.0004 0.0004 0.0003 0.0001 0.0007 0.0005 0.0009 0.0004 0.0002 0.001 0.0001 0.0003 0.0014 0.0004 0.0006 0.0009 0.0002 0.0004
Cytoplasm 0.632 0.788 0.7129 0.7549 0.6314 0.7238 0.4879 0.5034 0.4207 0.3323 0.4876 0.5102 0.3955 0.5655 0.4978 0.4445 0.375 0.6815
Cytoplasmic Foci 0.0224 0.0035 0.0106 0.0078 0.0121 0.0073 0.0225 0.0141 0.0194 0.03 0.0452 0.0156 0.019 0.0114 0.0185 0.0294 0.0236 0.0108
Eisosomes 0.0007 0 0.0001 0 0.0002 0.0001 0.0001 0 0 0.0003 0 0 0.0023 0.0001 0.0002 0.0001 0.0002 0.0001
Endoplasmic Reticulum 0.0169 0.0041 0.0105 0.0048 0.0041 0.0048 0.0079 0.0023 0.0032 0.0029 0.0029 0.0045 0.0194 0.0028 0.0054 0.0041 0.0078 0.0057
Endosome 0.0276 0.0022 0.0138 0.0222 0.0232 0.0081 0.0335 0.0073 0.0155 0.0304 0.03 0.011 0.0442 0.0027 0.0181 0.0203 0.0171 0.0102
Golgi 0.0048 0.0004 0.0054 0.0014 0.0052 0.0008 0.011 0.0017 0.005 0.0145 0.0098 0.0021 0.0181 0.0004 0.0081 0.0159 0.0154 0.0026
Lipid Particles 0.0065 0.0002 0.0024 0.0008 0.0362 0.0033 0.003 0.0004 0.0041 0.0019 0.0024 0.0013 0.0164 0.0004 0.003 0.0042 0.0095 0.0024
Mitochondria 0.0089 0.0002 0.0014 0.0053 0.0068 0.0008 0.0059 0.0012 0.0028 0.0136 0.0054 0.0025 0.0321 0.0008 0.0043 0.0402 0.0681 0.0058
None 0.0547 0.0382 0.0497 0.015 0.0059 0.0284 0.0321 0.0355 0.0435 0.0573 0.0417 0.0365 0.088 0.0966 0.0769 0.0166 0.0213 0.0467
Nuclear Periphery 0.0192 0.0049 0.0063 0.0078 0.0059 0.0048 0.0136 0.0045 0.006 0.0055 0.0065 0.005 0.0179 0.0078 0.008 0.0066 0.0184 0.0062
Nucleolus 0.0018 0.0016 0.0034 0.0033 0.0076 0.0032 0.004 0.0045 0.0035 0.0038 0.0055 0.0066 0.0025 0.0039 0.0035 0.0035 0.0048 0.0024
Nucleus 0.1176 0.1465 0.1534 0.1168 0.1929 0.1667 0.3016 0.3522 0.4194 0.4058 0.2958 0.3509 0.2126 0.2591 0.2642 0.3116 0.2392 0.174
Peroxisomes 0.0039 0.0001 0.0005 0.0004 0.0036 0.003 0.005 0.0016 0.0101 0.0117 0.0172 0.0062 0.0118 0.004 0.0077 0.0182 0.0631 0.0006
Punctate Nuclear 0.0313 0.0039 0.0026 0.0411 0.0112 0.0025 0.0152 0.0114 0.0151 0.0097 0.0274 0.0128 0.0068 0.0079 0.0118 0.0057 0.0585 0.0181
Vacuole 0.0045 0.0027 0.0029 0.0075 0.0056 0.0111 0.0133 0.0061 0.0053 0.0123 0.0037 0.006 0.0127 0.004 0.006 0.0187 0.0048 0.0045
Vacuole Periphery 0.0012 0.0002 0.0005 0.0026 0.0012 0.001 0.002 0.0005 0.0007 0.0022 0.0006 0.0008 0.0031 0.0005 0.0011 0.0026 0.0012 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.7335 8.3158 8.544 14.2075 9.2776 9.2972 14.3907 14.6237 15.153 17.7661
Translational Efficiency 2.0559 2.0552 1.9011 1.3551 1.9905 3.5327 1.4987 1.3225 1.6509 1.3235

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Ribosomal RNA processing element (RRPE)-binding protein; involved in the glucose-induced transition from quiescence to growth; restricted to nucleus in quiescent cells, released into cytoplasm after glucose repletion; binds Sin3p; relative distribution to the nucleus increases upon DNA replication stress
Localization
Cell Percentages cytoplasm (61%), nucleus (21%), mixed (18%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Stb3

Stb3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Stb3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available