Standard name | |
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Human Ortholog | |
Description | Glucosidase II beta subunit, forms a complex with alpha subunit Rot2p; involved in removal of two glucose residues from N-linked glycans during glycoprotein biogenesis in the ER; relocalizes from ER to cytoplasm upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 26 | 32 | 62 | 41 | 60 | 34 | 60 | 39 | 9 | 14 | 14 | 38 | 10 | 12 | 13 | 16 | 16 | 9 | 9 | 22 | 28 | 9 | 44 | 25 |
Bud | 2 | 10 | 9 | 9 | 11 | 23 | 31 | 23 | 35 | 34 | 39 | 29 | 1 | 3 | 2 | 0 | 4 | 0 | 0 | 1 | 2 | 1 | 1 | 1 |
Bud Neck | 0 | 0 | 4 | 0 | 3 | 2 | 1 | 1 | 0 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 9 | 2 | 3 | 2 |
Bud Site | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 1 | 24 | 18 | 11 | 16 | 16 | 18 | 13 | 13 | 10 | 14 | 8 | 22 | 28 | 22 | 15 | 9 | 0 | 2 | 12 | 7 | 28 | 23 |
Cytoplasm | 55 | 93 | 161 | 178 | 216 | 248 | 302 | 267 | 272 | 227 | 188 | 219 | 37 | 71 | 81 | 23 | 13 | 15 | 9 | 21 | 27 | 6 | 3 | 3 |
Endoplasmic Reticulum | 200 | 129 | 129 | 82 | 110 | 107 | 199 | 76 | 46 | 41 | 10 | 78 | 204 | 144 | 163 | 5 | 6 | 2 | 262 | 207 | 379 | 128 | 227 | 190 |
Endosome | 0 | 10 | 1 | 11 | 9 | 12 | 4 | 1 | 16 | 6 | 3 | 4 | 5 | 18 | 11 | 2 | 1 | 2 | 0 | 6 | 3 | 0 | 0 | 2 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 3 | 3 | 2 | 2 | 1 | 0 | 1 |
Mitochondria | 21 | 45 | 1 | 31 | 28 | 98 | 160 | 117 | 213 | 222 | 239 | 128 | 21 | 11 | 9 | 3 | 5 | 2 | 11 | 53 | 90 | 19 | 22 | 21 |
Nucleus | 1 | 0 | 1 | 0 | 2 | 0 | 2 | 9 | 3 | 7 | 9 | 4 | 1 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 3 | 1 | 0 | 0 | 1 | 0 | 2 | 4 | 0 | 6 | 4 | 0 | 9 | 0 | 3 | 0 | 0 | 0 | 2 | 2 | 4 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 3 | 0 | 0 | 0 | 6 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
SpindlePole | 2 | 0 | 4 | 3 | 7 | 5 | 9 | 8 | 5 | 10 | 1 | 13 | 0 | 4 | 0 | 3 | 4 | 0 | 0 | 1 | 2 | 0 | 0 | 0 |
Vac/Vac Membrane | 1 | 14 | 18 | 17 | 34 | 32 | 38 | 64 | 23 | 29 | 44 | 24 | 4 | 10 | 7 | 6 | 2 | 2 | 7 | 16 | 23 | 2 | 3 | 5 |
Unique Cell Count | 269 | 280 | 376 | 346 | 438 | 507 | 697 | 552 | 545 | 495 | 463 | 475 | 251 | 270 | 275 | 67 | 52 | 38 | 311 | 350 | 596 | 182 | 340 | 283 |
Labelled Cell Count | 315 | 336 | 415 | 390 | 492 | 578 | 825 | 630 | 639 | 618 | 568 | 553 | 300 | 303 | 318 | 83 | 68 | 45 | 311 | 350 | 596 | 182 | 340 | 283 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.2 | 5.4 | 6.2 | 5.9 | 6.3 | 5.1 | 5.5 | 5.7 | 4.8 | 4.7 | 4.4 | 5.3 | 6.2 | 6.6 | 6.5 | 9.4 | 8.7 | 9.6 | 5.7 | 5.8 | 6.4 |
Std Deviation (1e-4) | 0.7 | 0.8 | 0.7 | 0.7 | 1.0 | 0.7 | 0.9 | 1.0 | 0.8 | 1.0 | 0.7 | 0.9 | 0.8 | 1.2 | 1.0 | 1.5 | 1.6 | 2.3 | 1.0 | 1.0 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.08 | 0.02 | -0.28 | -0.19 | -0.13 | -0.38 | -0.4 | -0.51 | -0.24 | -0.01 | 0.07 | 0.06 | 0.6 | 0.48 | 0.62 | -0.14 | -0.1 | 0.03 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -1.8 | -1.1 | -4.6 | -3.9 | -4.5 | 0 | 0 | 0 | -3.8 | -4.8 | -4.7 | -4.6 | 1.5 | 2.5 | 1.1 |
Bud | 0.2 | 0.1 | 1.7 | 1.7 | 1.5 | 0 | 0 | 0 | 2.6 | 0 | -1.2 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.7 | -2.7 | -2.3 | -3.4 | -2.2 | 0 | 0 | 0 | -2.4 | -1.8 | 0.9 | 1.8 | 6.5 | 0 | 0 |
Cytoplasm | 2.3 | 1.9 | 1.8 | 0.2 | 1.7 | 2.1 | 0.9 | -0.6 | 1.0 | -7.4 | -4.3 | -3.5 | -1.3 | -2.5 | -0.4 |
Endoplasmic Reticulum | -3.1 | -2.9 | -4.4 | -2.0 | -7.4 | 0 | 0 | 0 | -6.0 | 11.5 | 4.8 | 6.3 | -4.4 | -3.3 | -3.7 |
Endosome | 3.1 | 0 | 2.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.7 | 3.5 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 5.7 | 4.7 | 8.9 | 9.9 | 9.4 | 0 | 0 | 0 | 10.8 | 5.4 | 3.5 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0.7 | 0 | 0 | 0 | 0 | 0 | 0 | 1.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.1 | 1.7 | 1.0 | 0.5 | 3.6 | -0.4 | 0.7 | 2.6 | 0.2 | -2.1 | -0.7 | -1.5 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.9295 | 1.1115 | 1.0706 | 1.2395 | 0.6343 | 1.0444 | 1.6739 | 1.9906 | 1.8361 | 1.8883 | 1.8875 | 1.771 | 1.8219 | 2.1146 | 1.8536 | 1.7866 | 1.6364 | 1.7392 |
Actin | 0.0115 | 0.0091 | 0.0119 | 0.0315 | 0.0072 | 0.0088 | 0.0368 | 0.0141 | 0.0381 | 0.0287 | 0.0063 | 0.0168 | 0.0436 | 0.005 | 0.0029 | 0.0436 | 0.0066 | 0.0148 |
Bud | 0.0011 | 0.001 | 0.0005 | 0.0004 | 0.0005 | 0.0003 | 0.0005 | 0.0005 | 0.0012 | 0.0008 | 0.0015 | 0.0004 | 0.0007 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 |
Bud Neck | 0.0024 | 0.0026 | 0.0097 | 0.0023 | 0.0019 | 0.007 | 0.0011 | 0.0015 | 0.0052 | 0.007 | 0.0021 | 0.0092 | 0.0019 | 0.0009 | 0.004 | 0.0148 | 0.0028 | 0.0059 |
Bud Periphery | 0.0026 | 0.0029 | 0.0014 | 0.0007 | 0.0013 | 0.0009 | 0.0011 | 0.0012 | 0.0039 | 0.0016 | 0.0085 | 0.0011 | 0.0017 | 0.0003 | 0.0003 | 0.001 | 0.0002 | 0.0002 |
Bud Site | 0.0052 | 0.0252 | 0.0183 | 0.0009 | 0.0014 | 0.0006 | 0.0033 | 0.0273 | 0.0094 | 0.0021 | 0.0014 | 0.0008 | 0.0038 | 0.0061 | 0.01 | 0.0023 | 0.0004 | 0.0003 |
Cell Periphery | 0.0077 | 0.0082 | 0.0083 | 0.0011 | 0.002 | 0.0038 | 0.0064 | 0.0149 | 0.0145 | 0.0052 | 0.0046 | 0.0046 | 0.0112 | 0.0102 | 0.0058 | 0.0121 | 0.0016 | 0.0018 |
Cytoplasm | 0.1859 | 0.2947 | 0.2514 | 0.3202 | 0.2855 | 0.3151 | 0.1499 | 0.2087 | 0.1948 | 0.2111 | 0.1367 | 0.2441 | 0.1495 | 0.2532 | 0.2066 | 0.1766 | 0.1678 | 0.2222 |
Cytoplasmic Foci | 0.0216 | 0.0246 | 0.0275 | 0.0269 | 0.0202 | 0.037 | 0.0405 | 0.0245 | 0.0269 | 0.0395 | 0.0442 | 0.026 | 0.0201 | 0.018 | 0.0288 | 0.0166 | 0.0183 | 0.0179 |
Eisosomes | 0.0006 | 0.0006 | 0.0006 | 0.0004 | 0.001 | 0.0006 | 0.0007 | 0.0012 | 0.0012 | 0.0007 | 0.0002 | 0.0003 | 0.0013 | 0.0004 | 0.0003 | 0.0007 | 0.0001 | 0.0002 |
Endoplasmic Reticulum | 0.5717 | 0.4114 | 0.4768 | 0.3467 | 0.385 | 0.4194 | 0.5167 | 0.5036 | 0.5032 | 0.4013 | 0.4361 | 0.54 | 0.5793 | 0.5441 | 0.5956 | 0.6034 | 0.6786 | 0.6163 |
Endosome | 0.0498 | 0.0595 | 0.0529 | 0.0408 | 0.0736 | 0.0431 | 0.0485 | 0.0474 | 0.0575 | 0.0474 | 0.1581 | 0.046 | 0.0331 | 0.0445 | 0.0376 | 0.0262 | 0.0126 | 0.0192 |
Golgi | 0.0175 | 0.01 | 0.0101 | 0.0071 | 0.0084 | 0.0087 | 0.0204 | 0.0243 | 0.0244 | 0.0408 | 0.0353 | 0.0127 | 0.011 | 0.0067 | 0.007 | 0.0128 | 0.0039 | 0.009 |
Lipid Particles | 0.0368 | 0.0354 | 0.034 | 0.0167 | 0.0206 | 0.0287 | 0.0671 | 0.0594 | 0.0458 | 0.0868 | 0.0606 | 0.0263 | 0.0563 | 0.043 | 0.0452 | 0.0114 | 0.0215 | 0.0212 |
Mitochondria | 0.0057 | 0.0044 | 0.0048 | 0.0091 | 0.0139 | 0.0141 | 0.0057 | 0.0069 | 0.0262 | 0.0156 | 0.0662 | 0.0063 | 0.0023 | 0.0009 | 0.0013 | 0.002 | 0.0037 | 0.0019 |
None | 0.0462 | 0.0687 | 0.0583 | 0.1222 | 0.1404 | 0.0847 | 0.0462 | 0.0382 | 0.0249 | 0.0735 | 0.0041 | 0.0412 | 0.0477 | 0.0454 | 0.0347 | 0.0477 | 0.0047 | 0.055 |
Nuclear Periphery | 0.0146 | 0.0209 | 0.0126 | 0.0382 | 0.0171 | 0.0098 | 0.0308 | 0.0117 | 0.0089 | 0.0068 | 0.0083 | 0.0118 | 0.0151 | 0.0087 | 0.0083 | 0.0106 | 0.0068 | 0.0056 |
Nucleolus | 0.0006 | 0.0005 | 0.0005 | 0.0007 | 0.0006 | 0.0003 | 0.0004 | 0.0003 | 0.0002 | 0.0006 | 0.0003 | 0.0002 | 0.0007 | 0.0001 | 0.0002 | 0.0001 | 0.0207 | 0.0001 |
Nucleus | 0.0022 | 0.0036 | 0.0039 | 0.015 | 0.0046 | 0.0048 | 0.003 | 0.0017 | 0.0014 | 0.0016 | 0.0018 | 0.0017 | 0.0042 | 0.0014 | 0.0013 | 0.0015 | 0.0114 | 0.0011 |
Peroxisomes | 0.0045 | 0.0007 | 0.0025 | 0.0026 | 0.0009 | 0.002 | 0.008 | 0.0008 | 0.0014 | 0.0164 | 0.0031 | 0.0008 | 0.0027 | 0.0003 | 0.0008 | 0.0007 | 0.0267 | 0.0015 |
Punctate Nuclear | 0.0013 | 0.0019 | 0.0024 | 0.0076 | 0.0022 | 0.0023 | 0.0022 | 0.0013 | 0.0012 | 0.002 | 0.0023 | 0.0011 | 0.0054 | 0.0009 | 0.0013 | 0.0006 | 0.0065 | 0.0007 |
Vacuole | 0.0088 | 0.0122 | 0.01 | 0.0075 | 0.0094 | 0.0067 | 0.0085 | 0.0094 | 0.0085 | 0.0091 | 0.0144 | 0.007 | 0.0067 | 0.0092 | 0.0071 | 0.0131 | 0.004 | 0.0044 |
Vacuole Periphery | 0.0016 | 0.0018 | 0.0015 | 0.0015 | 0.0023 | 0.0013 | 0.0024 | 0.0012 | 0.0014 | 0.0015 | 0.0037 | 0.0016 | 0.0015 | 0.0007 | 0.0007 | 0.0018 | 0.0011 | 0.0006 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 17.0036 | 20.111 | 18.7408 | 16.8039 | 13.5667 | 12.5496 | 26.0089 | 21.0651 | 19.7425 | 23.8099 |
Translational Efficiency | 0.7214 | 0.8282 | 0.7008 | 0.7956 | 0.7837 | 1.0743 | 0.6892 | 0.698 | 0.7202 | 0.6252 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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