Standard name
Human Ortholog
Description Glucosidase II beta subunit, forms a complex with alpha subunit Rot2p; involved in removal of two glucose residues from N-linked glycans during glycoprotein biogenesis in the ER; relocalizes from ER to cytoplasm upon DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.1 0.11 0.16 0.12 0.14 0.07 0.09 0.07 0 0 0 0.08 0 0 0.05 0.24 0.31 0.24 0 0.06 0.05 0.05 0.13 0.09
Bud 0 0 0 0 0 0.05 0 0 0.06 0.07 0.08 0.06 0 0 0 0 0.08 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0.08 0.1 0.33 0.29 0.24 0 0 0 0 0.08 0.08
Cytoplasm 0.2 0.33 0.43 0.51 0.49 0.49 0.43 0.48 0.5 0.46 0.41 0.46 0.15 0.26 0.29 0.34 0.25 0.39 0 0.06 0.05 0 0 0
Endoplasmic Reticulum 0.74 0.46 0.34 0.24 0.25 0.21 0.29 0.14 0.08 0.08 0 0.16 0.81 0.53 0.59 0.07 0.12 0.05 0.84 0.59 0.64 0.71 0.67 0.67
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0.05 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0
Mitochondria 0.08 0.16 0 0.09 0.06 0.19 0.23 0.21 0.39 0.45 0.52 0.27 0.08 0 0 0 0.1 0.05 0 0.15 0.15 0.1 0.07 0.08
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.05 0.05 0.05 0.08 0.06 0.05 0.12 0 0.06 0.1 0.05 0 0 0 0.09 0 0.05 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 26 32 62 41 60 34 60 39 9 14 14 38 10 12 13 16 16 9 9 22 28 9 44 25
Bud 2 10 9 9 11 23 31 23 35 34 39 29 1 3 2 0 4 0 0 1 2 1 1 1
Bud Neck 0 0 4 0 3 2 1 1 0 2 1 1 0 0 0 0 0 0 0 6 9 2 3 2
Bud Site 1 0 0 0 0 0 1 0 2 1 3 1 0 0 0 0 0 0
Cell Periphery 3 1 24 18 11 16 16 18 13 13 10 14 8 22 28 22 15 9 0 2 12 7 28 23
Cytoplasm 55 93 161 178 216 248 302 267 272 227 188 219 37 71 81 23 13 15 9 21 27 6 3 3
Endoplasmic Reticulum 200 129 129 82 110 107 199 76 46 41 10 78 204 144 163 5 6 2 262 207 379 128 227 190
Endosome 0 10 1 11 9 12 4 1 16 6 3 4 5 18 11 2 1 2 0 6 3 0 0 2
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 3 3 2 2 1 0 1
Mitochondria 21 45 1 31 28 98 160 117 213 222 239 128 21 11 9 3 5 2 11 53 90 19 22 21
Nucleus 1 0 1 0 2 0 2 9 3 7 9 4 1 1 0 1 1 1 0 0 0 0 0 1
Nuclear Periphery 3 1 0 0 1 0 2 4 0 6 4 0 9 0 3 0 0 0 2 2 4 0 0 0
Nucleolus 0 0 1 0 0 0 0 2 1 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 1 0 1 1 3 0 0 0 6 1 1 0 0 0 1 1 0 0 0
SpindlePole 2 0 4 3 7 5 9 8 5 10 1 13 0 4 0 3 4 0 0 1 2 0 0 0
Vac/Vac Membrane 1 14 18 17 34 32 38 64 23 29 44 24 4 10 7 6 2 2 7 16 23 2 3 5
Unique Cell Count 269 280 376 346 438 507 697 552 545 495 463 475 251 270 275 67 52 38 311 350 596 182 340 283
Labelled Cell Count 315 336 415 390 492 578 825 630 639 618 568 553 300 303 318 83 68 45 311 350 596 182 340 283


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.2 5.4 6.2 5.9 6.3 5.1 5.5 5.7 4.8 4.7 4.4 5.3 6.2 6.6 6.5 9.4 8.7 9.6 5.7 5.8 6.4
Std Deviation (1e-4) 0.7 0.8 0.7 0.7 1.0 0.7 0.9 1.0 0.8 1.0 0.7 0.9 0.8 1.2 1.0 1.5 1.6 2.3 1.0 1.0 1.3
Intensity Change (Log2) -0.08 0.02 -0.28 -0.19 -0.13 -0.38 -0.4 -0.51 -0.24 -0.01 0.07 0.06 0.6 0.48 0.62 -0.14 -0.1 0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.8 -1.1 -4.6 -3.9 -4.5 0 0 0 -3.8 -4.8 -4.7 -4.6 1.5 2.5 1.1
Bud 0.2 0.1 1.7 1.7 1.5 0 0 0 2.6 0 -1.2 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.7 -2.7 -2.3 -3.4 -2.2 0 0 0 -2.4 -1.8 0.9 1.8 6.5 0 0
Cytoplasm 2.3 1.9 1.8 0.2 1.7 2.1 0.9 -0.6 1.0 -7.4 -4.3 -3.5 -1.3 -2.5 -0.4
Endoplasmic Reticulum -3.1 -2.9 -4.4 -2.0 -7.4 0 0 0 -6.0 11.5 4.8 6.3 -4.4 -3.3 -3.7
Endosome 3.1 0 2.6 0 0 0 0 0 0 0 4.7 3.5 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 5.7 4.7 8.9 9.9 9.4 0 0 0 10.8 5.4 3.5 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0.7 0 0 0 0 0 0 1.7 0 0 0 0 0 0
Vacuole 0.1 1.7 1.0 0.5 3.6 -0.4 0.7 2.6 0.2 -2.1 -0.7 -1.5 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.9295 1.1115 1.0706 1.2395 0.6343 1.0444 1.6739 1.9906 1.8361 1.8883 1.8875 1.771 1.8219 2.1146 1.8536 1.7866 1.6364 1.7392
Actin 0.0115 0.0091 0.0119 0.0315 0.0072 0.0088 0.0368 0.0141 0.0381 0.0287 0.0063 0.0168 0.0436 0.005 0.0029 0.0436 0.0066 0.0148
Bud 0.0011 0.001 0.0005 0.0004 0.0005 0.0003 0.0005 0.0005 0.0012 0.0008 0.0015 0.0004 0.0007 0.0001 0.0001 0.0003 0.0001 0.0001
Bud Neck 0.0024 0.0026 0.0097 0.0023 0.0019 0.007 0.0011 0.0015 0.0052 0.007 0.0021 0.0092 0.0019 0.0009 0.004 0.0148 0.0028 0.0059
Bud Periphery 0.0026 0.0029 0.0014 0.0007 0.0013 0.0009 0.0011 0.0012 0.0039 0.0016 0.0085 0.0011 0.0017 0.0003 0.0003 0.001 0.0002 0.0002
Bud Site 0.0052 0.0252 0.0183 0.0009 0.0014 0.0006 0.0033 0.0273 0.0094 0.0021 0.0014 0.0008 0.0038 0.0061 0.01 0.0023 0.0004 0.0003
Cell Periphery 0.0077 0.0082 0.0083 0.0011 0.002 0.0038 0.0064 0.0149 0.0145 0.0052 0.0046 0.0046 0.0112 0.0102 0.0058 0.0121 0.0016 0.0018
Cytoplasm 0.1859 0.2947 0.2514 0.3202 0.2855 0.3151 0.1499 0.2087 0.1948 0.2111 0.1367 0.2441 0.1495 0.2532 0.2066 0.1766 0.1678 0.2222
Cytoplasmic Foci 0.0216 0.0246 0.0275 0.0269 0.0202 0.037 0.0405 0.0245 0.0269 0.0395 0.0442 0.026 0.0201 0.018 0.0288 0.0166 0.0183 0.0179
Eisosomes 0.0006 0.0006 0.0006 0.0004 0.001 0.0006 0.0007 0.0012 0.0012 0.0007 0.0002 0.0003 0.0013 0.0004 0.0003 0.0007 0.0001 0.0002
Endoplasmic Reticulum 0.5717 0.4114 0.4768 0.3467 0.385 0.4194 0.5167 0.5036 0.5032 0.4013 0.4361 0.54 0.5793 0.5441 0.5956 0.6034 0.6786 0.6163
Endosome 0.0498 0.0595 0.0529 0.0408 0.0736 0.0431 0.0485 0.0474 0.0575 0.0474 0.1581 0.046 0.0331 0.0445 0.0376 0.0262 0.0126 0.0192
Golgi 0.0175 0.01 0.0101 0.0071 0.0084 0.0087 0.0204 0.0243 0.0244 0.0408 0.0353 0.0127 0.011 0.0067 0.007 0.0128 0.0039 0.009
Lipid Particles 0.0368 0.0354 0.034 0.0167 0.0206 0.0287 0.0671 0.0594 0.0458 0.0868 0.0606 0.0263 0.0563 0.043 0.0452 0.0114 0.0215 0.0212
Mitochondria 0.0057 0.0044 0.0048 0.0091 0.0139 0.0141 0.0057 0.0069 0.0262 0.0156 0.0662 0.0063 0.0023 0.0009 0.0013 0.002 0.0037 0.0019
None 0.0462 0.0687 0.0583 0.1222 0.1404 0.0847 0.0462 0.0382 0.0249 0.0735 0.0041 0.0412 0.0477 0.0454 0.0347 0.0477 0.0047 0.055
Nuclear Periphery 0.0146 0.0209 0.0126 0.0382 0.0171 0.0098 0.0308 0.0117 0.0089 0.0068 0.0083 0.0118 0.0151 0.0087 0.0083 0.0106 0.0068 0.0056
Nucleolus 0.0006 0.0005 0.0005 0.0007 0.0006 0.0003 0.0004 0.0003 0.0002 0.0006 0.0003 0.0002 0.0007 0.0001 0.0002 0.0001 0.0207 0.0001
Nucleus 0.0022 0.0036 0.0039 0.015 0.0046 0.0048 0.003 0.0017 0.0014 0.0016 0.0018 0.0017 0.0042 0.0014 0.0013 0.0015 0.0114 0.0011
Peroxisomes 0.0045 0.0007 0.0025 0.0026 0.0009 0.002 0.008 0.0008 0.0014 0.0164 0.0031 0.0008 0.0027 0.0003 0.0008 0.0007 0.0267 0.0015
Punctate Nuclear 0.0013 0.0019 0.0024 0.0076 0.0022 0.0023 0.0022 0.0013 0.0012 0.002 0.0023 0.0011 0.0054 0.0009 0.0013 0.0006 0.0065 0.0007
Vacuole 0.0088 0.0122 0.01 0.0075 0.0094 0.0067 0.0085 0.0094 0.0085 0.0091 0.0144 0.007 0.0067 0.0092 0.0071 0.0131 0.004 0.0044
Vacuole Periphery 0.0016 0.0018 0.0015 0.0015 0.0023 0.0013 0.0024 0.0012 0.0014 0.0015 0.0037 0.0016 0.0015 0.0007 0.0007 0.0018 0.0011 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 17.0036 20.111 18.7408 16.8039 13.5667 12.5496 26.0089 21.0651 19.7425 23.8099
Translational Efficiency 0.7214 0.8282 0.7008 0.7956 0.7837 1.0743 0.6892 0.698 0.7202 0.6252

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Glucosidase II beta subunit, forms a complex with alpha subunit Rot2p; involved in removal of two glucose residues from N-linked glycans during glycoprotein biogenesis in the ER; relocalizes from ER to cytoplasm upon DNA replication stress
Localization
Cell Percentages ER (98%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Gtb1

Gtb1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gtb1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available