Standard name | |
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Human Ortholog | |
Description | Protein kinase; phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 1 | 0 | 0 | 1 | 1 |
Bud | 0 | 0 | 2 | 2 | 1 | 1 | 2 | 3 | 3 | 8 | 1 | 2 | 1 | 0 | 1 | – | – | – | 0 | 0 | 0 | 0 | 0 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 1 | 0 | 0 | 1 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 0 | 4 | 0 | 1 | 0 | 0 | 0 | 1 | 3 | 3 | 1 | 1 | 1 | 0 | 0 | – | – | – | 0 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 41 | 119 | 76 | 106 | 113 | 117 | 133 | 172 | 96 | 140 | 61 | 83 | 98 | 107 | 110 | – | – | – | 68 | 132 | 71 | 60 | 86 | 84 |
Endoplasmic Reticulum | 1 | 3 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 1 | 4 | 1 | 1 | 0 | 0 | – | – | – | 1 | 1 | 1 | 1 | 1 | 8 |
Endosome | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 1 | 0 | 1 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | – | – | 1 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3 | 16 | 31 | 44 | 39 | 58 | 62 | 90 | 48 | 107 | 57 | 73 | 11 | 19 | 42 | – | – | – | 3 | 9 | 19 | 1 | 6 | 8 |
Nucleus | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 3 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | – | 0 | 2 | 0 | 1 | 2 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 2 | 0 | 2 | 0 | 0 | 1 | 3 | 1 | 0 | 1 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 2 | 0 | 1 | 2 | 0 | 2 | 1 | 2 | 5 | 4 | 4 | 2 | 1 | 0 | – | – | – | 0 | 7 | 3 | 2 | 2 | 5 |
Unique Cell Count | 43 | 131 | 88 | 124 | 134 | 146 | 169 | 225 | 123 | 199 | 106 | 119 | 105 | 116 | 116 | 76 | 160 | 105 | 71 | 108 | 125 | |||
Labelled Cell Count | 46 | 146 | 109 | 157 | 157 | 179 | 202 | 275 | 155 | 268 | 129 | 165 | 115 | 128 | 153 | 76 | 160 | 105 | 71 | 108 | 125 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.1 | 5.0 | 4.1 | 5.0 | 4.8 | 4.4 | 4.7 | 4.5 | 4.7 | 4.4 | 4.3 | 4.1 | 5.4 | 5.1 | 4.6 | – | – | – | 5.2 | 6.1 | 6.9 |
Std Deviation (1e-4) | 0.8 | 1.0 | 0.6 | 2.2 | 1.8 | 1.5 | 2.2 | 2.2 | 2.3 | 2.4 | 1.9 | 2.3 | 1.4 | 1.2 | 1.4 | – | – | – | 1.1 | 1.8 | 2.1 |
Intensity Change (Log2) | – | – | – | 0.31 | 0.23 | 0.11 | 0.2 | 0.15 | 0.21 | 0.11 | 0.06 | 0.01 | 0.4 | 0.32 | 0.17 | – | – | – | 0.34 | 0.58 | 0.75 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cytoplasm | -0.2 | -0.4 | -1.2 | -1.5 | -1.9 | -1.5 | -2.9 | -4.4 | -2.8 | 1.6 | 1.4 | 2.1 | – | – | – |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -4.2 | -3.1 | 0 | – | – | – |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0806 | 0.0055 | 0.0016 | 0.4239 | 0.0012 | 0.0776 | 0.0961 | 0.0001 | 0.0033 | 0.0769 | 0.0001 | 0.0001 | – | – | – | – | – | – |
Bud | 0.0017 | 0.001 | 0.0005 | 0.0033 | 0.0001 | 0.001 | 0.0006 | 0.0001 | 0.0005 | 0.0016 | 0.0001 | 0.0001 | – | – | – | – | – | – |
Bud Neck | 0.0104 | 0.0005 | 0.0003 | 0.0082 | 0.0008 | 0.0028 | 0.0036 | 0 | 0.0007 | 0.0017 | 0.0001 | 0.0004 | – | – | – | – | – | – |
Bud Periphery | 0.0041 | 0.0028 | 0.0008 | 0.0055 | 0 | 0.0038 | 0.0007 | 0 | 0.0005 | 0.0031 | 0 | 0 | – | – | – | – | – | – |
Bud Site | 0.0373 | 0.0133 | 0.0015 | 0.0251 | 0.0002 | 0.0017 | 0.0049 | 0.0003 | 0.0056 | 0.0068 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0.0086 | 0.0076 | 0.0012 | 0.0037 | 0.0001 | 0.0024 | 0.0003 | 0.0001 | 0.0002 | 0.0006 | 0 | 0 | – | – | – | – | – | – |
Cytoplasm | 0.4485 | 0.8009 | 0.7334 | 0.0141 | 0.8757 | 0.4751 | 0.3202 | 0.7237 | 0.6974 | 0.4749 | 0.8693 | 0.7389 | – | – | – | – | – | – |
Cytoplasmic Foci | 0.0056 | 0.0078 | 0.0073 | 0.0426 | 0.0381 | 0.0218 | 0.0204 | 0.0038 | 0.0169 | 0.0293 | 0.0056 | 0.0088 | – | – | – | – | – | – |
Eisosomes | 0.0027 | 0.0014 | 0.0001 | 0.004 | 0 | 0.0019 | 0.0006 | 0 | 0.0006 | 0.0082 | 0 | 0 | – | – | – | – | – | – |
Endoplasmic Reticulum | 0.028 | 0.0129 | 0.0232 | 0.0519 | 0.0215 | 0.045 | 0.0069 | 0.0025 | 0.0032 | 0.0041 | 0.0055 | 0.0048 | – | – | – | – | – | – |
Endosome | 0.0225 | 0.0068 | 0.0189 | 0.1654 | 0.0277 | 0.0529 | 0.093 | 0.0028 | 0.0181 | 0.0074 | 0.006 | 0.0062 | – | – | – | – | – | – |
Golgi | 0.008 | 0.0011 | 0.0012 | 0.1122 | 0.0018 | 0.0136 | 0.0224 | 0.0002 | 0.0022 | 0.0069 | 0.0002 | 0.0003 | – | – | – | – | – | – |
Lipid Particles | 0.0082 | 0.001 | 0.0009 | 0.0733 | 0.0011 | 0.0237 | 0.0167 | 0.0001 | 0.0073 | 0.0068 | 0 | 0.0001 | – | – | – | – | – | – |
Mitochondria | 0.0235 | 0.0071 | 0.0038 | 0.0193 | 0.0004 | 0.0235 | 0.0132 | 0.0001 | 0.0021 | 0.0097 | 0.0001 | 0.0003 | – | – | – | – | – | – |
None | 0.147 | 0.1209 | 0.1638 | 0.0078 | 0.0241 | 0.1964 | 0.3729 | 0.2636 | 0.225 | 0.3242 | 0.1095 | 0.2374 | – | – | – | – | – | – |
Nuclear Periphery | 0.0796 | 0.0011 | 0.0131 | 0.0135 | 0.0019 | 0.0217 | 0.0049 | 0.0004 | 0.0013 | 0.0023 | 0.0006 | 0.0004 | – | – | – | – | – | – |
Nucleolus | 0.0028 | 0.0002 | 0.0004 | 0.0007 | 0 | 0.0025 | 0.0004 | 0 | 0.0021 | 0.0038 | 0 | 0 | – | – | – | – | – | – |
Nucleus | 0.0497 | 0.0012 | 0.015 | 0.0023 | 0.0017 | 0.0055 | 0.0023 | 0.0005 | 0.0016 | 0.0027 | 0.0007 | 0.0005 | – | – | – | – | – | – |
Peroxisomes | 0.0026 | 0.0001 | 0.0002 | 0.0078 | 0.0003 | 0.0048 | 0.0056 | 0 | 0.005 | 0.0214 | 0 | 0 | – | – | – | – | – | – |
Punctate Nuclear | 0.0131 | 0.0003 | 0.0012 | 0.0055 | 0.001 | 0.0061 | 0.0032 | 0.0001 | 0.0034 | 0.0054 | 0.0001 | 0.0002 | – | – | – | – | – | – |
Vacuole | 0.0114 | 0.0062 | 0.0098 | 0.0069 | 0.002 | 0.0106 | 0.0085 | 0.0012 | 0.0025 | 0.0016 | 0.0016 | 0.001 | – | – | – | – | – | – |
Vacuole Periphery | 0.0043 | 0.0004 | 0.0018 | 0.0032 | 0.0003 | 0.0057 | 0.0025 | 0.0002 | 0.0006 | 0.0006 | 0.0002 | 0.0002 | – | – | – | – | – | – |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 23.2646 | 20.401 | 13.3747 | 20.9509 | 17.3799 | 15.2797 | 20.0767 | 20.1397 | 19.4733 | 23.2765 |
Translational Efficiency | 0.3161 | 0.3318 | 0.4419 | 0.3229 | 0.3367 | 0.4887 | 0.3841 | 0.2533 | 0.3524 | 0.2926 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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