Standard name
Human Ortholog
Description Protein kinase; phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.95 0.91 0.86 0.85 0.84 0.8 0.79 0.76 0.78 0.7 0.58 0.7 0.93 0.92 0.95 0.9 0.83 0.68 0.85 0.8 0.67
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0.12 0.35 0.35 0.29 0.4 0.37 0.4 0.39 0.54 0.54 0.61 0.1 0.16 0.36 0 0.06 0.19 0 0.06 0.07
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 1
Bud 0 0 2 2 1 1 2 3 3 8 1 2 1 0 1 0 0 0 0 0 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0
Bud Site 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cell Periphery 0 4 0 1 0 0 0 1 3 3 1 1 1 0 0 0 0 1 0 0 0
Cytoplasm 41 119 76 106 113 117 133 172 96 140 61 83 98 107 110 68 132 71 60 86 84
Endoplasmic Reticulum 1 3 0 0 1 1 0 2 0 1 4 1 1 0 0 1 1 1 1 1 8
Endosome 0 0 0 1 0 0 2 1 0 0 0 0 0 0 0 0 0 1 0 1 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0
Mitochondria 3 16 31 44 39 58 62 90 48 107 57 73 11 19 42 3 9 19 1 6 8
Nucleus 1 1 0 0 0 0 1 3 1 0 0 1 0 0 0 0 2 0 1 2 0
Nuclear Periphery 0 1 0 2 0 2 0 0 1 3 1 0 1 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 2 0 1 2 0 2 1 2 5 4 4 2 1 0 0 7 3 2 2 5
Unique Cell Count 43 131 88 124 134 146 169 225 123 199 106 119 105 116 116 76 160 105 71 108 125
Labelled Cell Count 46 146 109 157 157 179 202 275 155 268 129 165 115 128 153 76 160 105 71 108 125


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.1 5.0 4.1 5.0 4.8 4.4 4.7 4.5 4.7 4.4 4.3 4.1 5.4 5.1 4.6 5.2 6.1 6.9
Std Deviation (1e-4) 0.8 1.0 0.6 2.2 1.8 1.5 2.2 2.2 2.3 2.4 1.9 2.3 1.4 1.2 1.4 1.1 1.8 2.1
Intensity Change (Log2) 0.31 0.23 0.11 0.2 0.15 0.21 0.11 0.06 0.01 0.4 0.32 0.17 0.34 0.58 0.75


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.2 -0.4 -1.2 -1.5 -1.9 -1.5 -2.9 -4.4 -2.8 1.6 1.4 2.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -4.2 -3.1 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin 0.0806 0.0055 0.0016 0.4239 0.0012 0.0776 0.0961 0.0001 0.0033 0.0769 0.0001 0.0001
Bud 0.0017 0.001 0.0005 0.0033 0.0001 0.001 0.0006 0.0001 0.0005 0.0016 0.0001 0.0001
Bud Neck 0.0104 0.0005 0.0003 0.0082 0.0008 0.0028 0.0036 0 0.0007 0.0017 0.0001 0.0004
Bud Periphery 0.0041 0.0028 0.0008 0.0055 0 0.0038 0.0007 0 0.0005 0.0031 0 0
Bud Site 0.0373 0.0133 0.0015 0.0251 0.0002 0.0017 0.0049 0.0003 0.0056 0.0068 0 0
Cell Periphery 0.0086 0.0076 0.0012 0.0037 0.0001 0.0024 0.0003 0.0001 0.0002 0.0006 0 0
Cytoplasm 0.4485 0.8009 0.7334 0.0141 0.8757 0.4751 0.3202 0.7237 0.6974 0.4749 0.8693 0.7389
Cytoplasmic Foci 0.0056 0.0078 0.0073 0.0426 0.0381 0.0218 0.0204 0.0038 0.0169 0.0293 0.0056 0.0088
Eisosomes 0.0027 0.0014 0.0001 0.004 0 0.0019 0.0006 0 0.0006 0.0082 0 0
Endoplasmic Reticulum 0.028 0.0129 0.0232 0.0519 0.0215 0.045 0.0069 0.0025 0.0032 0.0041 0.0055 0.0048
Endosome 0.0225 0.0068 0.0189 0.1654 0.0277 0.0529 0.093 0.0028 0.0181 0.0074 0.006 0.0062
Golgi 0.008 0.0011 0.0012 0.1122 0.0018 0.0136 0.0224 0.0002 0.0022 0.0069 0.0002 0.0003
Lipid Particles 0.0082 0.001 0.0009 0.0733 0.0011 0.0237 0.0167 0.0001 0.0073 0.0068 0 0.0001
Mitochondria 0.0235 0.0071 0.0038 0.0193 0.0004 0.0235 0.0132 0.0001 0.0021 0.0097 0.0001 0.0003
None 0.147 0.1209 0.1638 0.0078 0.0241 0.1964 0.3729 0.2636 0.225 0.3242 0.1095 0.2374
Nuclear Periphery 0.0796 0.0011 0.0131 0.0135 0.0019 0.0217 0.0049 0.0004 0.0013 0.0023 0.0006 0.0004
Nucleolus 0.0028 0.0002 0.0004 0.0007 0 0.0025 0.0004 0 0.0021 0.0038 0 0
Nucleus 0.0497 0.0012 0.015 0.0023 0.0017 0.0055 0.0023 0.0005 0.0016 0.0027 0.0007 0.0005
Peroxisomes 0.0026 0.0001 0.0002 0.0078 0.0003 0.0048 0.0056 0 0.005 0.0214 0 0
Punctate Nuclear 0.0131 0.0003 0.0012 0.0055 0.001 0.0061 0.0032 0.0001 0.0034 0.0054 0.0001 0.0002
Vacuole 0.0114 0.0062 0.0098 0.0069 0.002 0.0106 0.0085 0.0012 0.0025 0.0016 0.0016 0.001
Vacuole Periphery 0.0043 0.0004 0.0018 0.0032 0.0003 0.0057 0.0025 0.0002 0.0006 0.0006 0.0002 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 23.2646 20.401 13.3747 20.9509 17.3799 15.2797 20.0767 20.1397 19.4733 23.2765
Translational Efficiency 0.3161 0.3318 0.4419 0.3229 0.3367 0.4887 0.3841 0.2533 0.3524 0.2926

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Protein kinase; phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control
Localization
Cell Percentages cytoplasm (60%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Gcn2

Gcn2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gcn2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available