Standard name | |
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Human Ortholog | |
Description | Transmembrane protein; subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-S homolog |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 10 | 8 | 4 | 5 | 7 | 6 | 20 | 9 | 12 | 9 | 9 | 5 | 10 | 5 | 26 | 18 | 22 | 2 | 6 | 5 | 1 | 5 | 8 |
Bud | 0 | 0 | 2 | 2 | 5 | 8 | 16 | 7 | 6 | 16 | 6 | 3 | 0 | 0 | 0 | 2 | 0 | 2 | 1 | 5 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 1 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 1 | 1 | 1 | 3 | 8 | 6 | 12 | 12 | 25 | 13 | 11 | 4 | 31 | 16 | 40 | 30 | 40 | 0 | 0 | 0 | 1 | 4 | 9 |
Cytoplasm | 18 | 36 | 34 | 13 | 27 | 46 | 27 | 45 | 35 | 58 | 27 | 25 | 13 | 31 | 27 | 100 | 49 | 36 | 0 | 10 | 5 | 3 | 2 | 4 |
Endoplasmic Reticulum | 24 | 191 | 94 | 84 | 72 | 70 | 69 | 117 | 97 | 126 | 67 | 75 | 65 | 146 | 90 | 14 | 1 | 4 | 136 | 242 | 141 | 55 | 113 | 129 |
Endosome | 6 | 1 | 1 | 0 | 5 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 11 | 2 | 1 | 0 | 4 | 2 | 0 | 1 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 11 | 39 | 13 | 0 | 5 | 1 | 0 | 1 | 1 |
Mitochondria | 23 | 8 | 40 | 46 | 55 | 129 | 156 | 112 | 100 | 195 | 83 | 89 | 2 | 5 | 7 | 22 | 13 | 8 | 4 | 4 | 11 | 2 | 2 | 5 |
Nucleus | 0 | 1 | 2 | 0 | 1 | 3 | 2 | 3 | 4 | 4 | 0 | 2 | 0 | 1 | 0 | 2 | 2 | 2 | 0 | 0 | 0 | 1 | 1 | 0 |
Nuclear Periphery | 1 | 9 | 9 | 13 | 9 | 13 | 16 | 15 | 15 | 15 | 7 | 7 | 2 | 5 | 2 | 1 | 3 | 0 | 2 | 4 | 6 | 0 | 1 | 0 |
Nucleolus | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 45 | 35 | 19 | 7 | 61 | 49 | 54 | 33 | 30 | 46 | 16 | 14 | 0 | 3 | 8 | 104 | 63 | 41 | 2 | 9 | 7 | 2 | 4 | 4 |
Unique Cell Count | 104 | 255 | 169 | 129 | 191 | 247 | 260 | 283 | 223 | 353 | 164 | 166 | 81 | 190 | 131 | 293 | 189 | 148 | 152 | 299 | 188 | 72 | 143 | 169 |
Labelled Cell Count | 122 | 292 | 211 | 170 | 244 | 335 | 356 | 366 | 309 | 500 | 228 | 237 | 91 | 232 | 155 | 336 | 222 | 169 | 152 | 299 | 188 | 72 | 143 | 169 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.9 | 9.1 | 7.1 | 6.8 | 7.1 | 6.5 | 5.8 | 6.9 | 6.8 | 6.3 | 6.4 | 6.6 | 9.0 | 9.2 | 8.7 | 9.6 | 11.9 | 12.0 | 7.7 | 8.7 | 9.7 |
Std Deviation (1e-4) | 1.9 | 2.2 | 1.9 | 1.6 | 1.5 | 1.6 | 1.4 | 1.6 | 1.5 | 1.4 | 1.3 | 1.7 | 2.0 | 2.2 | 2.4 | 2.6 | 3.0 | 3.0 | 1.5 | 2.1 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.06 | -0.0 | -0.14 | -0.3 | -0.06 | -0.08 | -0.17 | -0.17 | -0.11 | 0.34 | 0.37 | 0.28 | 0.43 | 0.74 | 0.75 | 0.12 | 0.28 | 0.45 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.7 | -1.1 | -1.0 | -1.4 | 1.0 | -0.3 | -0.7 | 0.3 | 0.3 | 0 | 0.2 | -0.4 | 1.6 | 1.7 | 3.1 |
Bud | 0 | 0 | 0 | 2.5 | 0 | 0 | 2.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 2.6 | 3.2 | 3.3 | 3.0 | 0 | 5.2 | 4.3 | 4.8 | 5.1 | 7.0 |
Cytoplasm | -2.4 | -1.5 | -0.4 | -2.8 | -1.1 | -1.1 | -1.0 | -0.9 | -1.2 | -0.8 | -0.9 | 0.1 | 3.2 | 1.3 | 0.9 |
Endoplasmic Reticulum | 1.7 | -3.4 | -5.6 | -6.1 | -2.9 | -2.4 | -4.3 | -2.7 | -1.9 | 3.8 | 4.3 | 2.3 | -12.4 | -11.8 | -10.2 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6.3 | 3.9 |
Mitochondria | 2.3 | 1.1 | 5.8 | 7.4 | 3.5 | 4.3 | 6.8 | 5.1 | 5.6 | -4.2 | -6.0 | -4.3 | -4.9 | -4.5 | -4.5 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1.6 | -0.3 | 0 | 0.4 | 0 | 0.6 | -0.5 | -0.5 | -0.5 | 0 | -1.3 | 0 | 0 | -2.0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.8 | 4.7 | 2.3 | 2.6 | 0.1 | 0.7 | 0.6 | -0.4 | -0.9 | -3.1 | -3.8 | -1.5 | 5.7 | 5.0 | 3.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.7751 | 5.174 | 5.0691 | 5.8209 | 9.1021 | 5.151 | 3.6614 | 4.6706 | 4.7568 | 4.1224 | 3.1993 | 5.0494 | 5.4307 | 5.4348 | 5.609 | 5.4337 | 5.5714 | 5.8577 |
Actin | 0 | 0.0012 | 0.0011 | 0.0006 | 0.0023 | 0.0046 | 0.002 | 0.0021 | 0.029 | 0.0123 | 0.0474 | 0.0035 | 0.0063 | 0.0117 | 0.0162 | 0.0067 | 0.0029 | 0.0132 |
Bud | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0003 | 0.0004 | 0.0003 | 0.0002 | 0.0004 | 0 |
Bud Neck | 0 | 0.0006 | 0.0092 | 0.0001 | 0.0002 | 0.003 | 0.0002 | 0.0001 | 0.0005 | 0.002 | 0.0007 | 0.0004 | 0.0006 | 0.0014 | 0.0004 | 0.0003 | 0.0007 | 0.0004 |
Bud Periphery | 0 | 0.0001 | 0.0003 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0007 | 0.0004 | 0.0002 | 0.0004 | 0.0001 | 0.0005 | 0.0015 | 0.0007 | 0.0003 | 0.0006 | 0.0001 |
Bud Site | 0 | 0.008 | 0.0004 | 0 | 0.0002 | 0.0002 | 0.0003 | 0.0017 | 0.0034 | 0.0006 | 0.0016 | 0 | 0.0024 | 0.0171 | 0.0009 | 0.0004 | 0.0023 | 0 |
Cell Periphery | 0.0001 | 0.0159 | 0.0055 | 0.0003 | 0.0004 | 0.0025 | 0.0005 | 0.004 | 0.0024 | 0.0006 | 0.0005 | 0.0003 | 0.0008 | 0.0032 | 0.0012 | 0.0007 | 0.0004 | 0.0005 |
Cytoplasm | 0.0012 | 0.2391 | 0.1763 | 0.033 | 0.0138 | 0.0836 | 0.0556 | 0.2001 | 0.0415 | 0.165 | 0.1312 | 0.0629 | 0.1263 | 0.1249 | 0.0792 | 0.1127 | 0.0911 | 0.0801 |
Cytoplasmic Foci | 0.0001 | 0.0169 | 0.0032 | 0.0008 | 0.0019 | 0.0038 | 0.0241 | 0.0058 | 0.0069 | 0.0344 | 0.0307 | 0.0061 | 0.0277 | 0.0108 | 0.0053 | 0.0033 | 0.0332 | 0.0044 |
Eisosomes | 0 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0003 | 0.0023 | 0.0003 | 0.0001 | 0.0008 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.927 | 0.5912 | 0.6696 | 0.9402 | 0.9426 | 0.8265 | 0.6739 | 0.612 | 0.7589 | 0.5579 | 0.5077 | 0.7731 | 0.6654 | 0.725 | 0.8084 | 0.7739 | 0.6019 | 0.82 |
Endosome | 0.0035 | 0.087 | 0.0041 | 0.0047 | 0.0115 | 0.0061 | 0.0362 | 0.0059 | 0.0381 | 0.0459 | 0.0406 | 0.0172 | 0.0408 | 0.0114 | 0.0093 | 0.0339 | 0.0895 | 0.0047 |
Golgi | 0.0001 | 0.0047 | 0.0011 | 0.0003 | 0.0025 | 0.0017 | 0.036 | 0.0012 | 0.0195 | 0.0646 | 0.0502 | 0.0137 | 0.012 | 0.0025 | 0.0042 | 0.0111 | 0.0225 | 0.0005 |
Lipid Particles | 0.0014 | 0.0157 | 0.006 | 0.0008 | 0.0019 | 0.0071 | 0.0317 | 0.0062 | 0.0065 | 0.0075 | 0.0536 | 0.0066 | 0.0137 | 0.0099 | 0.0066 | 0.0033 | 0.0324 | 0.0043 |
Mitochondria | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0009 | 0.0004 | 0.0033 | 0.0005 | 0.0177 | 0.0094 | 0.0802 | 0.0026 | 0.0042 | 0.001 | 0.0013 | 0.0094 | 0.0045 | 0.0001 |
None | 0.0004 | 0.0011 | 0.1103 | 0.0013 | 0.0005 | 0.0014 | 0.0869 | 0.142 | 0.0474 | 0.0668 | 0.0193 | 0.0802 | 0.0233 | 0.0139 | 0.0317 | 0.0213 | 0.032 | 0.0416 |
Nuclear Periphery | 0.0606 | 0.0106 | 0.0076 | 0.0132 | 0.0169 | 0.0447 | 0.0129 | 0.0081 | 0.0125 | 0.0077 | 0.0082 | 0.0262 | 0.0404 | 0.0405 | 0.0256 | 0.0152 | 0.0457 | 0.0247 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0005 | 0.0002 | 0.0002 | 0.0001 | 0.0004 | 0 |
Nucleus | 0.0005 | 0.0012 | 0.0013 | 0.0013 | 0.0009 | 0.0017 | 0.0009 | 0.001 | 0.001 | 0.001 | 0.0008 | 0.0012 | 0.0091 | 0.0083 | 0.0023 | 0.0012 | 0.0064 | 0.0016 |
Peroxisomes | 0 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0111 | 0.0001 | 0.0063 | 0.0191 | 0.0193 | 0.0004 | 0.0109 | 0.0034 | 0.0008 | 0.0001 | 0.0023 | 0.0001 |
Punctate Nuclear | 0.0001 | 0.0012 | 0.0003 | 0.0003 | 0.0002 | 0.0004 | 0.0206 | 0.0006 | 0.0006 | 0.001 | 0.0017 | 0.0004 | 0.0027 | 0.0032 | 0.0011 | 0.0003 | 0.018 | 0.0003 |
Vacuole | 0.0026 | 0.0046 | 0.0027 | 0.0025 | 0.0022 | 0.0102 | 0.0023 | 0.0048 | 0.0047 | 0.0028 | 0.0034 | 0.0037 | 0.0068 | 0.0067 | 0.0027 | 0.0038 | 0.0104 | 0.0027 |
Vacuole Periphery | 0.0022 | 0.0005 | 0.0002 | 0.0004 | 0.0007 | 0.0015 | 0.0007 | 0.0004 | 0.0016 | 0.0007 | 0.0011 | 0.0013 | 0.005 | 0.0025 | 0.0014 | 0.0017 | 0.0024 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 28.8741 | 17.2078 | 19.6094 | 34.5569 | 24.9577 | 20.613 | 33.8655 | 35.8581 | 29.7731 | 43.5309 |
Translational Efficiency | 1.4649 | 1.8815 | 1.6278 | 0.7706 | 1.201 | 1.7209 | 1.0876 | 0.6825 | 0.9684 | 0.7988 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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