Standard name
Human Ortholog
Description Putative mitochondrial NAD+ transporter; member of the mitochondrial carrier subfamily (see also YIA6); has putative human ortholog; YEA6 has a paralog, YIA6, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0.06 0.07 0.05 0 0.07 0.06 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.54 0.34 0.31 0.3 0.21 0.25 0.28 0.23 0.19 0.26 0.23 0.19 0.32 0.53 0.62 0.82 0.83 0.82 0.12 0 0.07 0 0 0
Endoplasmic Reticulum 0.1 0.08 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.08 0.09 0.08 0.1 0.05 0 0 0 0
Endosome 0.1 0.08 0 0 0 0 0 0 0 0 0 0 0.11 0.13 0.15 0 0 0.07 0.07 0.11 0.06 0.08 0.08 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.16 0.43 0.68 0.71 0.78 0.8 0.75 0.79 0.83 0.76 0.82 0.85 0.29 0.16 0 0.12 0 0.08 0.56 0.62 0.68 0.67 0.6 0.58
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.05 0.05 0.06 0.07 0.06 0.11 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.08 0.14 0 0 0.06 0 0 0 0 0 0 0 0.2 0.13 0.14 0 0.07 0 0.08 0.08 0.06 0.1 0.12 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 2 2 0 0 0 0 0 0 0 0 0 4 9 0 4 0 0 3 5 4 0 13 14
Bud 6 6 5 7 29 38 35 28 42 40 29 33 4 4 3 0 0 0 0 1 0 0 7 7
Bud Neck 0 0 0 0 0 1 2 1 2 0 1 0 3 1 1 1 1 0 1 2 2 1 9 11
Bud Site 0 0 0 2 3 14 9 8 11 13 4 9 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 4 1 2 1 1 1 2 4 3 1 5 6 6 4 3 4 1 0 1 0 0 0 1
Cytoplasm 90 53 45 86 100 142 183 147 107 160 112 107 43 172 213 99 101 97 30 9 12 6 6 13
Endoplasmic Reticulum 17 13 4 2 6 2 1 0 2 0 2 6 6 9 8 10 11 9 25 11 4 3 7 4
Endosome 17 13 0 12 21 5 8 1 2 4 1 1 15 41 51 3 4 8 17 25 10 17 34 27
Golgi 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2 2 5 2 2 4 10 4
Mitochondria 26 67 100 202 378 455 486 499 469 473 403 486 38 50 15 14 5 9 143 147 125 143 257 239
Nucleus 0 1 0 1 2 12 6 9 9 7 6 7 0 1 2 3 0 0 0 1 0 0 4 2
Nuclear Periphery 0 0 1 0 1 0 1 0 3 1 1 4 0 0 0 0 0 0 0 0 0 0 2 1
Nucleolus 0 0 0 0 0 0 1 2 0 4 2 3 0 0 2 0 0 1 0 0 0 1 1 3
Peroxisomes 0 2 0 11 11 5 3 3 1 5 1 2 4 12 34 0 0 1 0 1 2 4 7 6
SpindlePole 7 2 2 10 22 30 39 41 35 66 22 21 9 11 12 2 0 3 4 3 2 3 6 7
Vac/Vac Membrane 13 22 4 9 28 15 19 15 10 17 13 11 26 43 48 1 8 5 19 19 11 21 49 58
Unique Cell Count 167 156 146 285 486 566 647 629 564 619 489 573 133 322 346 120 122 119 258 237 185 216 427 412
Labelled Cell Count 186 186 164 344 602 720 794 756 697 793 598 695 158 359 393 142 136 136 258 237 185 216 427 412


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 5.0 3.6 3.7 3.8 3.3 3.1 3.2 3.0 2.8 2.9 2.9 5.8 5.3 5.5 5.3 5.5 5.9 6.1 6.2 6.4
Std Deviation (1e-4) 0.5 0.9 0.7 1.3 1.6 1.2 1.8 1.3 1.3 1.1 1.3 1.0 1.8 0.9 1.5 1.0 1.1 2.0 1.3 1.6 2.1
Intensity Change (Log2) – – – 0.07 0.08 -0.12 -0.2 -0.18 -0.24 -0.33 -0.27 -0.32 0.7 0.58 0.63 0.57 0.63 0.73 0.78 0.79 0.84


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.1 -2.6 -1.4 -0.6 -1.9 -3.1 -1.2 -1.9 -3.2 0.3 4.5 6.2 8.4 8.5 8.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 2.0 2.2 1.8
Endosome 0 0 0 0 0 0 0 0 0 4.2 4.5 4.9 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -6.7 -11.4 -15.4 -9.3 -10.8 -10.0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 3.9 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 2.3 0 0 0 0 0
Vacuole 0 1.5 0 0 0 0 0 0 0 4.5 3.5 3.7 0 1.5 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.1283 0.9799 0.9153 1.3507 0.4327 0.9973 1.2397 1.4859 1.7744 1.7069 1.9131 1.7421 -0.7884 -0.025 -0.474 -0.3418 -0.6791 -0.5134
Actin 0.0086 0.0081 0.0123 0.0295 0.0072 0.0094 0.0291 0.0191 0.0512 0.0358 0.029 0.0125 0.0104 0.012 0.0261 0.0248 0.0292 0.0041
Bud 0.0096 0.0082 0.0051 0.0046 0.003 0.0044 0.0018 0.0063 0.0032 0.0028 0.0025 0.0011 0.001 0.0021 0.0019 0.0056 0.0024 0.0019
Bud Neck 0.0064 0.0022 0.0053 0.0032 0.0017 0.0093 0.0057 0.0015 0.0158 0.0022 0.0044 0.0093 0.0076 0.0012 0.0012 0.0035 0.0033 0.0063
Bud Periphery 0.0044 0.0092 0.0063 0.0122 0.0084 0.0056 0.0033 0.0172 0.0107 0.0104 0.0148 0.0023 0.0016 0.0033 0.0032 0.0127 0.005 0.0041
Bud Site 0.0283 0.0545 0.0199 0.0193 0.0238 0.003 0.017 0.0459 0.0256 0.0034 0.0064 0.0018 0.0148 0.0164 0.0067 0.0081 0.0082 0.0025
Cell Periphery 0.0013 0.0013 0.0011 0.0016 0.0006 0.0011 0.0027 0.0034 0.0057 0.0012 0.0013 0.0008 0.0016 0.0012 0.001 0.0014 0.0005 0.0006
Cytoplasm 0.0304 0.0791 0.0389 0.0349 0.039 0.0549 0.0461 0.0403 0.0576 0.0218 0.0138 0.0259 0.0205 0.0756 0.0341 0.0379 0.0079 0.037
Cytoplasmic Foci 0.1075 0.1164 0.1294 0.1001 0.097 0.114 0.1338 0.1246 0.1078 0.0787 0.1185 0.0918 0.1007 0.1348 0.1076 0.0711 0.0807 0.1006
Eisosomes 0.0008 0.0003 0.0007 0.0005 0.0003 0.0003 0.0011 0.001 0.003 0.0013 0.0005 0.0003 0.0005 0.0007 0.0007 0.0002 0.0004 0.0003
Endoplasmic Reticulum 0.0039 0.0042 0.0038 0.003 0.0039 0.0036 0.0158 0.0035 0.0119 0.0015 0.0019 0.0059 0.0105 0.0087 0.0063 0.0027 0.0035 0.0053
Endosome 0.181 0.1813 0.1626 0.1632 0.228 0.1554 0.1808 0.121 0.111 0.1207 0.1643 0.16 0.248 0.1911 0.1797 0.0948 0.1817 0.1611
Golgi 0.0762 0.074 0.0613 0.0706 0.0983 0.0639 0.0755 0.0903 0.0694 0.0533 0.0985 0.098 0.0951 0.0887 0.0729 0.0412 0.1185 0.0769
Lipid Particles 0.0689 0.017 0.0245 0.045 0.0138 0.0248 0.0556 0.0187 0.0221 0.0055 0.0745 0.0278 0.0753 0.0244 0.0231 0.0229 0.0276 0.046
Mitochondria 0.2881 0.3059 0.3715 0.3121 0.3534 0.389 0.2674 0.3818 0.3746 0.5618 0.3208 0.447 0.2774 0.345 0.4074 0.6103 0.3961 0.442
None 0.0826 0.0672 0.0557 0.1007 0.0405 0.0612 0.0665 0.0395 0.0492 0.0553 0.016 0.033 0.0326 0.0193 0.0383 0.0024 0.0025 0.0074
Nuclear Periphery 0.0035 0.0024 0.0018 0.0016 0.0054 0.0018 0.0125 0.0006 0.0027 0.0004 0.001 0.0021 0.0113 0.0015 0.0022 0.0011 0.012 0.002
Nucleolus 0.0036 0.0053 0.0016 0.0013 0.0016 0.0026 0.0035 0.0005 0.0011 0.0003 0.0008 0.0005 0.0047 0.0006 0.001 0.0005 0.0029 0.0005
Nucleus 0.0032 0.0067 0.0036 0.0017 0.0038 0.0035 0.0043 0.0008 0.0014 0.0005 0.001 0.0007 0.0026 0.0011 0.0021 0.0012 0.003 0.001
Peroxisomes 0.06 0.0341 0.0751 0.0774 0.0496 0.0743 0.0518 0.0746 0.0589 0.0313 0.1012 0.0643 0.0531 0.0548 0.0614 0.0441 0.0826 0.0836
Punctate Nuclear 0.0097 0.0042 0.0051 0.0019 0.0029 0.0036 0.008 0.0007 0.0006 0.0003 0.0029 0.001 0.0018 0.001 0.0012 0.002 0.0191 0.0012
Vacuole 0.0155 0.0138 0.0102 0.0093 0.0117 0.0093 0.0132 0.0058 0.0125 0.0067 0.0173 0.0086 0.0203 0.0118 0.0146 0.0073 0.0071 0.0099
Vacuole Periphery 0.0067 0.0047 0.0044 0.0063 0.0061 0.0049 0.0046 0.003 0.0041 0.0048 0.0087 0.0054 0.0085 0.0048 0.0074 0.0042 0.0061 0.0058

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.0617 14.6782 10.8919 18.8821 17.7407 10.9404 19.5408 15.0251 19.8689 20.1943
Translational Efficiency 0.5787 0.54 0.7256 0.4741 0.5647 0.7999 0.5819 0.8593 0.6866 0.6991

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Putative mitochondrial NAD+ transporter; member of the mitochondrial carrier subfamily (see also YIA6); has putative human ortholog; YEA6 has a paralog, YIA6, that arose from the whole genome duplication
Localization
Cell Percentages mitochondrion (59%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Yea6

Yea6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yea6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available