ORF | |
---|---|
Human Ortholog | |
Description | Putative protein of unknown function; conserved among S. cerevisiae strains; YEL008W is not an essential gene; predicted to be involved in metabolism |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 3 |
Bud | 1 | 0 | 3 | 1 | 2 | 0 | – | 2 | 3 | 2 | 6 | 7 | 0 | 2 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 4 | 14 |
Bud Neck | 0 | 0 | 0 | 1 | 2 | 0 | – | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 6 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 167 | 170 | 58 | 136 | 221 | 161 | – | 280 | 158 | 246 | 148 | 282 | 391 | 488 | 681 | 78 | 79 | 48 | 164 | 161 | 66 | 93 | 333 | 404 |
Endoplasmic Reticulum | 1 | 0 | 0 | 0 | 1 | 1 | – | 0 | 1 | 2 | 2 | 0 | 0 | 2 | 0 | 5 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 2 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 1 | 3 | 0 | 1 | 2 | 0 | 0 | 2 | 4 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 2 |
Mitochondria | 5 | 0 | 1 | 15 | 15 | 49 | – | 116 | 109 | 130 | 122 | 197 | 0 | 2 | 1 | 5 | 4 | 2 | 1 | 1 | 0 | 1 | 7 | 5 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 2 | – | 2 | 0 | 3 | 2 | 2 | 1 | 2 | 2 | 0 | 2 | 1 | 0 | 1 | 0 | 0 | 2 | 3 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 0 | – | 2 | 0 | 4 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 3 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 1 | 1 | – | 1 | 3 | 0 | 0 | 4 | 7 | 0 | 5 | 1 | 3 | 0 | 2 | 0 | 0 | 0 | 3 | 7 |
Unique Cell Count | 172 | 171 | 59 | 141 | 228 | 174 | 329 | 199 | 318 | 217 | 361 | 396 | 490 | 689 | 86 | 87 | 50 | 175 | 175 | 70 | 98 | 362 | 465 | |
Labelled Cell Count | 176 | 171 | 62 | 153 | 243 | 214 | 405 | 275 | 389 | 283 | 497 | 403 | 497 | 693 | 102 | 97 | 53 | 175 | 175 | 70 | 98 | 362 | 465 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.3 | 5.2 | 3.1 | 3.6 | 4.0 | 3.3 | – | 3.2 | 3.2 | 3.0 | 2.9 | 2.9 | 5.8 | 5.7 | 5.5 | 5.4 | 5.5 | 5.4 | 4.7 | 5.1 | 5.9 |
Std Deviation (1e-4) | 0.6 | 0.8 | 0.5 | 1.3 | 1.3 | 1.3 | – | 1.0 | 2.0 | 0.9 | 1.2 | 1.1 | 6.4 | 1.4 | 1.5 | 1.1 | 1.4 | 1.3 | 1.0 | 1.3 | 1.9 |
Intensity Change (Log2) | – | – | – | 0.18 | 0.35 | 0.09 | – | 0.04 | 0.0 | -0.08 | -0.13 | -0.11 | 0.87 | 0.85 | 0.8 | 0.77 | 0.8 | 0.78 | 0.59 | 0.71 | 0.9 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.7 | -0.6 | -1.6 | – | -2.8 | -3.5 | -3.7 | -4.7 | -3.7 | 0.3 | 1.3 | 0.4 | -1.9 | -1.8 | -0.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.7147 | 5.2945 | 4.8012 | 3.8548 | 3.8952 | 4.4467 | 2.9147 | 4.492 | 3.8641 | 3.2716 | 3.4398 | 3.58 | 4.4056 | 5.8563 | 5.709 | 4.5199 | 4.6488 | 5.1262 |
Actin | 0.0068 | 0.0005 | 0.009 | 0.0004 | 0.0047 | 0.0026 | 0.0232 | 0.0006 | 0.0112 | 0.0019 | 0.0102 | 0.0036 | 0.0003 | 0.0003 | 0.0004 | 0.0016 | 0.0062 | 0.0002 |
Bud | 0.0007 | 0.0018 | 0.0005 | 0.0001 | 0.0016 | 0.0002 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0006 | 0.0006 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 |
Bud Neck | 0.0127 | 0.0003 | 0.0009 | 0.0004 | 0.0008 | 0.002 | 0.0012 | 0.0001 | 0.0005 | 0.0003 | 0.0006 | 0.0011 | 0.0001 | 0.0001 | 0.0002 | 0.0005 | 0.0009 | 0.0009 |
Bud Periphery | 0.0012 | 0.0023 | 0.0004 | 0 | 0.0025 | 0.0003 | 0.0003 | 0 | 0.0002 | 0.0002 | 0.0009 | 0.0006 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 |
Bud Site | 0.0059 | 0.0026 | 0.003 | 0.0001 | 0.0014 | 0.0003 | 0.0034 | 0.0003 | 0.0013 | 0.0002 | 0.0007 | 0.0004 | 0.0001 | 0.0003 | 0.0002 | 0.0002 | 0.0004 | 0 |
Cell Periphery | 0.0006 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0 |
Cytoplasm | 0.8382 | 0.9567 | 0.9119 | 0.9088 | 0.789 | 0.9375 | 0.8328 | 0.9595 | 0.9228 | 0.8959 | 0.8407 | 0.9414 | 0.9253 | 0.976 | 0.9701 | 0.9678 | 0.7951 | 0.9545 |
Cytoplasmic Foci | 0.0351 | 0.0032 | 0.0103 | 0.0152 | 0.0109 | 0.0079 | 0.0175 | 0.0066 | 0.0086 | 0.0046 | 0.011 | 0.0096 | 0.0023 | 0.0018 | 0.0019 | 0.0035 | 0.0032 | 0.0032 |
Eisosomes | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0003 | 0 |
Endoplasmic Reticulum | 0.0047 | 0.0018 | 0.0027 | 0.0016 | 0.0036 | 0.0024 | 0.0051 | 0.0016 | 0.0019 | 0.0018 | 0.0012 | 0.0031 | 0.004 | 0.001 | 0.001 | 0.0015 | 0.0045 | 0.0009 |
Endosome | 0.0149 | 0.0016 | 0.0208 | 0.0348 | 0.0852 | 0.0079 | 0.0215 | 0.0066 | 0.0101 | 0.0051 | 0.0262 | 0.0057 | 0.0008 | 0.0004 | 0.0004 | 0.0009 | 0.0116 | 0.0011 |
Golgi | 0.0084 | 0.0002 | 0.005 | 0.0178 | 0.008 | 0.0031 | 0.0082 | 0.0023 | 0.0064 | 0.003 | 0.0217 | 0.0006 | 0 | 0 | 0 | 0.0001 | 0.0008 | 0.0001 |
Lipid Particles | 0.0083 | 0.0001 | 0.0006 | 0.0012 | 0.0022 | 0.001 | 0.0157 | 0.0004 | 0.0021 | 0.0029 | 0.0121 | 0.0017 | 0 | 0 | 0 | 0 | 0.0011 | 0 |
Mitochondria | 0.0069 | 0.001 | 0.0025 | 0.0009 | 0.015 | 0.0097 | 0.0029 | 0.0003 | 0.0066 | 0.0402 | 0.034 | 0.0052 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0013 | 0.0005 |
None | 0.024 | 0.0188 | 0.0189 | 0.0079 | 0.0035 | 0.0102 | 0.0362 | 0.0125 | 0.0162 | 0.0294 | 0.0066 | 0.0071 | 0.0584 | 0.016 | 0.0187 | 0.0182 | 0.0396 | 0.0295 |
Nuclear Periphery | 0.0014 | 0.0009 | 0.0014 | 0.0009 | 0.0093 | 0.0017 | 0.0086 | 0.0009 | 0.0009 | 0.0013 | 0.0093 | 0.0024 | 0.0012 | 0.0004 | 0.0007 | 0.0007 | 0.0542 | 0.0006 |
Nucleolus | 0.0008 | 0.0001 | 0.0001 | 0 | 0.0005 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0005 | 0.0003 | 0 | 0 | 0 | 0 | 0.003 | 0 |
Nucleus | 0.0048 | 0.0047 | 0.0044 | 0.0075 | 0.0536 | 0.0092 | 0.0077 | 0.0059 | 0.006 | 0.0101 | 0.0077 | 0.0088 | 0.0064 | 0.0031 | 0.0054 | 0.0037 | 0.0565 | 0.0075 |
Peroxisomes | 0.0174 | 0.0002 | 0.0009 | 0.0007 | 0.001 | 0.0004 | 0.0061 | 0.001 | 0.0034 | 0.0009 | 0.0084 | 0.0008 | 0 | 0 | 0 | 0.0001 | 0.0003 | 0.0001 |
Punctate Nuclear | 0.0014 | 0.0021 | 0.0006 | 0.0004 | 0.0012 | 0.0015 | 0.0059 | 0.0006 | 0.0005 | 0.0007 | 0.0023 | 0.0059 | 0.0003 | 0.0003 | 0.0005 | 0.0004 | 0.018 | 0.0004 |
Vacuole | 0.0054 | 0.0008 | 0.0054 | 0.0011 | 0.0033 | 0.0014 | 0.0018 | 0.0004 | 0.0008 | 0.0008 | 0.0019 | 0.0007 | 0.0003 | 0.0002 | 0.0002 | 0.0003 | 0.0015 | 0.0003 |
Vacuole Periphery | 0.0004 | 0.0002 | 0.0006 | 0.0003 | 0.0024 | 0.0004 | 0.0008 | 0.0001 | 0.0002 | 0.0006 | 0.003 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0.0012 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | – | – | – | – | – | – | – | – | – | – |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | – |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|