| Standard name | |
|---|---|
| Human Ortholog | |
| Description | Component of the INO80 chromatin remodeling complex; critical for INO80 function; involved in regulation of chromosome segregation and maintenance of normal centromeric chromatin structure; human ortholog INO80C is a member of the human INO80 complex; implicated in DNA repair based on genetic interactions with RAD52 epistasis genes |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | – | 0 | 0 | 0 | 0 | 0 | – | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
| Bud | – | 0 | 0 | 0 | 0 | 0 | – | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 5 | 1 | 0 | 0 | 0 |
| Bud Neck | – | 0 | 0 | 0 | 0 | 0 | – | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
| Bud Site | – | 0 | 0 | 0 | 0 | 0 | – | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | 0 | 0 | 0 | 0 | 0 | – | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
| Cytoplasm | – | 12 | 2 | 4 | 2 | 5 | – | 5 | – | – | – | – | – | – | – | – | – | – | 0 | 2 | 0 | 0 | 0 | 0 |
| Endoplasmic Reticulum | – | 0 | 2 | 0 | 0 | 1 | – | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
| Endosome | – | 1 | 1 | 0 | 0 | 0 | – | 1 | – | – | – | – | – | – | – | – | – | – | 0 | 1 | 5 | 0 | 0 | 0 |
| Golgi | – | 0 | 0 | 0 | 0 | 1 | – | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 1 | 1 | 0 | 0 | 0 |
| Mitochondria | – | 0 | 8 | 0 | 0 | 0 | – | 1 | – | – | – | – | – | – | – | – | – | – | 0 | 1 | 2 | 0 | 0 | 0 |
| Nucleus | – | 115 | 30 | 13 | 14 | 12 | – | 12 | – | – | – | – | – | – | – | – | – | – | 15 | 115 | 56 | 3 | 3 | 0 |
| Nuclear Periphery | – | 0 | 0 | 0 | 0 | 0 | – | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
| Nucleolus | – | 4 | 1 | 5 | 3 | 1 | – | 2 | – | – | – | – | – | – | – | – | – | – | 0 | 4 | 0 | 0 | 0 | 0 |
| Peroxisomes | – | 0 | 0 | 0 | 0 | 0 | – | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
| SpindlePole | – | 0 | 0 | 0 | 0 | 0 | – | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 1 | 0 | 0 | 0 |
| Vac/Vac Membrane | – | 10 | 3 | 3 | 0 | 0 | – | 1 | – | – | – | – | – | – | – | – | – | – | 0 | 18 | 8 | 1 | 0 | 0 |
| Unique Cell Count | 140 | 41 | 20 | 17 | 19 | 22 | 19 | 155 | 80 | 5 | 6 | 1 | ||||||||||||
| Labelled Cell Count | 142 | 47 | 25 | 19 | 20 | 22 | 19 | 155 | 80 | 5 | 6 | 1 | ||||||||||||
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | – | 10.9 | 5.3 | 6.7 | 7.1 | 6.7 | – | 7.1 | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Std Deviation (1e-4) | – | 2.6 | 1.3 | 1.8 | 1.2 | 1.6 | – | 1.9 | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Intensity Change (Log2) | – | – | – | 0.33 | 0.42 | 0.32 | – | 0.42 | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Cortical Patches | 0 | 0 | 0 | – | 0 | – | – | – | – | – | – | – | – | – | – |
| Bud | 0 | 0 | 0 | – | 0 | – | – | – | – | – | – | – | – | – | – |
| Bud Neck | 0 | 0 | 0 | – | 0 | – | – | – | – | – | – | – | – | – | – |
| Bud Site | 0 | 0 | 0 | – | 0 | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | 0 | 0 | 0 | – | 0 | – | – | – | – | – | – | – | – | – | – |
| Cytoplasm | 0 | 0 | 0 | – | 0 | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | – | – | – | – | – | – | – | – | – | – |
| Endosome | 0 | 0 | 0 | – | 0 | – | – | – | – | – | – | – | – | – | – |
| Golgi | 0 | 0 | 0 | – | 0 | – | – | – | – | – | – | – | – | – | – |
| Mitochondria | 0 | 0 | 0 | – | 0 | – | – | – | – | – | – | – | – | – | – |
| Nucleus | -0.7 | 0.7 | -0.8 | – | -1.5 | – | – | – | – | – | – | – | – | – | – |
| Nuclear Periphery | 0 | 0 | 0 | – | 0 | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | 0 | 0 | 0 | – | 0 | – | – | – | – | – | – | – | – | – | – |
| Peroxisomes | 0 | 0 | 0 | – | 0 | – | – | – | – | – | – | – | – | – | – |
| SpindlePole | 0 | 0 | 0 | – | 0 | – | – | – | – | – | – | – | – | – | – |
| Vacuole | 0 | 0 | 0 | – | 0 | – | – | – | – | – | – | – | – | – | – |
External localization resources
Images
Protein Concentration and Protein Localization Data
| R1 | R2 | R3 | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
| Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Actin | 0.0151 | 0.0002 | 0.006 | 0 | 0.0011 | 0 | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | 0.0014 | 0 | 0.0001 | 0 | 0.0001 | 0 | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Neck | 0.0123 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0014 | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Periphery | 0.0026 | 0 | 0.0003 | 0 | 0.0002 | 0.0001 | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Site | 0.0294 | 0.0001 | 0.0007 | 0 | 0.0001 | 0.0001 | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | 0.0019 | 0 | 0.0001 | 0 | 0.0001 | 0 | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasm | 0.0049 | 0.0001 | 0.0035 | 0.0001 | 0.0001 | 0 | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasmic Foci | 0.0203 | 0.0002 | 0.0004 | 0 | 0.0013 | 0 | – | – | – | – | – | – | – | – | – | – | – | – |
| Eisosomes | 0.0013 | 0 | 0.0004 | 0 | 0.0001 | 0 | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | 0.0043 | 0 | 0.0016 | 0 | 0.0001 | 0 | – | – | – | – | – | – | – | – | – | – | – | – |
| Endosome | 0.0345 | 0.0003 | 0.0065 | 0 | 0.0062 | 0 | – | – | – | – | – | – | – | – | – | – | – | – |
| Golgi | 0.0147 | 0.0004 | 0.0008 | 0 | 0.0168 | 0 | – | – | – | – | – | – | – | – | – | – | – | – |
| Lipid Particles | 0.1681 | 0.0005 | 0.0007 | 0 | 0.0066 | 0 | – | – | – | – | – | – | – | – | – | – | – | – |
| Mitochondria | 0.09 | 0.0044 | 0.0258 | 0.0001 | 0.3627 | 0.0001 | – | – | – | – | – | – | – | – | – | – | – | – |
| None | 0.0041 | 0 | 0.0001 | 0 | 0.0001 | 0 | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuclear Periphery | 0.1003 | 0.0024 | 0.0039 | 0.0016 | 0.0007 | 0.0003 | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | 0.0473 | 0.005 | 0.0032 | 0.0025 | 0.0089 | 0.0394 | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleus | 0.2645 | 0.9854 | 0.9403 | 0.9955 | 0.5903 | 0.958 | – | – | – | – | – | – | – | – | – | – | – | – |
| Peroxisomes | 0.085 | 0.0003 | 0.0019 | 0 | 0.0017 | 0 | – | – | – | – | – | – | – | – | – | – | – | – |
| Punctate Nuclear | 0.0771 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole | 0.0107 | 0.0002 | 0.0024 | 0 | 0.0006 | 0.0003 | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole Periphery | 0.0101 | 0.0002 | 0.0008 | 0 | 0.0021 | 0.0001 | – | – | – | – | – | – | – | – | – | – | – | – |
Sequencing Data
| R1 | R2 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
| Gene Expression | 49.5541 | 61.0333 | 49.6721 | 44.2232 | 44.2605 | 33.0178 | 55.241 | 53.7426 | 54.7038 | 48.8269 |
| Translational Efficiency | 1.675 | 1.714 | 1.482 | 1.5934 | 2.0972 | 2.147 | 1.4209 | 1.591 | 1.1378 | 1.5059 |
Hit Data
| Dataset | Hit |
|---|---|
| Protein Concentration | – |
| Protein Localization | ✘ |
| Gene Expression | ✘ |
| Translational Efficiency | ✘ |
| Standard name | |
|---|---|
| Human Ortholog | |
| Description | Component of the INO80 chromatin remodeling complex; critical for INO80 function; involved in regulation of chromosome segregation and maintenance of normal centromeric chromatin structure; human ortholog INO80C is a member of the human INO80 complex; implicated in DNA repair based on genetic interactions with RAD52 epistasis genes |
| Localization | |
| Cell Percentages | nucleus (98%) |
| Cell Cycle Regulation | No |
| Subcompartmental Group | nucleus-4 |
Ies6
Ies6
Nearest neighbours ( genes)
correlation threshold
0.80
Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)
Endocytosis
| Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
|---|---|---|---|---|
| 37℃ | ||||
| RT |
Cell Cycle Omics
CYCLoPs (Ies6-GFP)
| Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
|---|
| Gene | Images |
|---|
| Gene | Images |
|---|
Images are not yet available
Images are not yet available
