Standard name
Human Ortholog
Description Non-essential INO80 chromatin remodeling complex subunit; deletion affects telomere maintenance via recombination
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0.07 0.14 0.13 0.07 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.16 0.14 0.09 0.15 0.17 0.16 0.31 0.22 0.29 0.31 0 0 0 0 0.05 0 0 0 0 0 0 0
Nucleus 0.93 0.94 0.84 0.9 0.85 0.85 0.78 0.78 0.69 0.74 0.74 0.69 0.93 0.9 0.8 0.77 0.84 0.78 0.9 0.91 0.89 0.82 0.79 0.71
Nuclear Periphery 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.16 0.13 0.05 0.19 0.2 0.29 0.29 0.33 0.43 0.39 0.4 0.4 0.17 0.16 0.12 0.1 0.08 0.16 0 0 0 0.09 0.14 0.13
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Bud 0 0 0 0 0 0 0 1 0 3 1 2 0 1 0 0 1 0 2 1 0 0 2 3
Bud Neck 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 2 3 1 2 6 4 14 4 10 1 1 2 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 2 1 8 13 5 13 12 4 5 1 10 13 41 82 9 7 6 0 1 0 0 0 2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 0 0 0 0 0 0 0 0
Endosome 1 1 0 0 0 0 0 0 0 0 0 0 0 2 4 0 1 0 6 3 0 3 2 4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 4
Mitochondria 9 3 3 27 30 34 56 71 84 83 62 87 7 4 2 0 5 3 3 1 1 0 0 3
Nucleus 273 232 16 173 277 200 253 339 188 281 157 194 408 504 468 55 87 57 277 234 53 70 325 349
Nuclear Periphery 0 0 1 3 3 3 7 5 4 2 6 7 0 0 1 1 0 0 0 0 0 0 1 1
Nucleolus 47 31 1 37 65 69 95 143 118 149 85 111 75 87 73 7 8 12 8 8 2 7 56 64
Peroxisomes 1 0 0 0 0 0 0 1 0 0 0 0 0 2 5 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 0 1 0 0 1 2 10 0 0 0 1 0 0 0 3 3
Vac/Vac Membrane 4 3 0 2 3 0 2 1 1 3 0 2 5 2 4 2 1 3 5 5 1 1 17 52
Unique Cell Count 294 247 19 193 326 234 326 437 272 379 213 280 438 560 588 71 103 73 308 259 60 86 414 493
Labelled Cell Count 340 272 22 252 394 312 428 581 403 542 316 423 510 647 651 76 110 81 308 259 60 86 414 493


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.5 9.2 5.1 6.3 6.5 5.5 5.5 5.5 4.9 5.2 4.9 5.0 9.0 9.0 8.6 14.2 14.2 12.4 7.7 8.8 9.5
Std Deviation (1e-4) 1.9 1.8 1.1 1.6 1.6 1.4 1.9 1.5 1.4 1.5 1.2 1.6 2.0 2.2 2.6 4.8 4.8 3.4 2.1 2.0 2.3
Intensity Change (Log2) – – – 0.31 0.36 0.12 0.13 0.13 -0.05 0.05 -0.05 -0.01 0.82 0.82 0.76 1.49 1.49 1.29 0.61 0.81 0.91


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 1.4 0 1.2 1.4 0 0 0 0 0 0
Nucleus 0.7 0.1 0.1 -0.7 -0.7 -1.4 -1.0 -1.0 -1.4 1.5 0.8 -0.5 -0.6 0 -0.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 2.3 2.3 2.5 3.3 3.0 3.0 3.0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.8817 3.7518 3.7668 3.1821 2.6214 3.9997 4.2032 3.9443 3.6263 3.1692 3.3296 3.4131 6.1833 6.4733 5.7894 5.3001 5.3332 5.7052
Actin 0.0036 0.0004 0.0078 0.0056 0.1082 0 0.0282 0 0.013 0 0 0.0001 0.0415 0 0.008 0.0072 0.001 0.0002
Bud 0.0007 0 0.0001 0.0003 0.0013 0 0.0007 0 0.0001 0 0 0.0001 0.0003 0 0.0002 0.0002 0.0004 0
Bud Neck 0.0016 0 0.0001 0.0004 0.0012 0.0005 0.0018 0 0.0001 0 0.0002 0.0007 0.0006 0 0.0009 0.0001 0.0124 0.002
Bud Periphery 0.0022 0 0.0001 0.0005 0.002 0.0001 0.002 0 0.0001 0 0.0001 0.0002 0.0006 0 0.0008 0.0003 0.0007 0.0001
Bud Site 0.0068 0.0001 0.0022 0.0005 0.0063 0 0.0025 0 0.0004 0 0.0001 0.0002 0.0009 0 0.0019 0.0007 0.0049 0.0001
Cell Periphery 0.0006 0 0 0.0001 0.0005 0 0.0009 0 0 0 0 0.0001 0.0001 0 0.0007 0.0001 0.0007 0
Cytoplasm 0.0009 0.0005 0.0001 0.0001 0.0013 0 0.0011 0 0.0002 0 0 0.0001 0.0042 0 0.0006 0.0018 0.0007 0.0001
Cytoplasmic Foci 0.0244 0.0025 0.0022 0.0187 0.0541 0 0.0083 0 0.0026 0 0 0.0011 0.0208 0 0.0035 0.0121 0.0051 0.0055
Eisosomes 0 0 0.0001 0.0001 0.0005 0 0.0007 0 0.0001 0 0 0 0.0002 0 0.0005 0.0002 0.0001 0
Endoplasmic Reticulum 0.0016 0.0002 0.0001 0.0002 0.0014 0 0.0014 0 0.0004 0 0 0 0.0058 0 0.0018 0.0014 0.0004 0
Endosome 0.0094 0.0082 0.0008 0.0179 0.0732 0 0.0144 0 0.0025 0 0 0.0012 0.014 0 0.0018 0.0034 0.0083 0.0005
Golgi 0.0041 0.0038 0.001 0.0315 0.0823 0 0.0073 0 0.004 0 0 0.0011 0.0122 0 0.0022 0.0015 0.0035 0.0006
Lipid Particles 0.0128 0.0003 0.0015 0.0464 0.0793 0 0.0158 0 0.0031 0 0 0.0037 0.0139 0 0.0118 0.007 0.0139 0.0011
Mitochondria 0.0029 0.001 0.0008 0.1086 0.1578 0.0001 0.0123 0 0.0046 0 0.0006 0.0013 0.0056 0.0001 0.0078 0.0301 0.0056 0.0002
None 0.0004 0.0001 0.0001 0.0001 0.0008 0 0.0023 0 0.0002 0 0 0 0.0096 0 0.0014 0.0071 0.0007 0
Nuclear Periphery 0.0095 0.0001 0.0002 0.0002 0.0023 0.0001 0.0039 0.0001 0.0032 0 0.0009 0.0004 0.0182 0.0003 0.0015 0.0019 0.0095 0.0003
Nucleolus 0.0253 0.0173 0.0167 0.0029 0.0438 0.0786 0.0449 0.018 0.0209 0.0315 0.1607 0.0982 0.0249 0.0225 0.0189 0.0076 0.0971 0.084
Nucleus 0.8636 0.9646 0.964 0.7472 0.358 0.9203 0.8377 0.9816 0.9426 0.9683 0.8366 0.8863 0.795 0.9762 0.9249 0.9104 0.8011 0.8991
Peroxisomes 0.0226 0.0004 0.0017 0.0168 0.0181 0 0.0046 0 0.0013 0 0 0.0009 0.0116 0 0.0074 0.002 0.0111 0.005
Punctate Nuclear 0.002 0.0003 0.0002 0.0007 0.0013 0.0001 0.0026 0.0001 0.0002 0 0.0005 0.0038 0.018 0.0008 0.0025 0.0035 0.0152 0.0009
Vacuole 0.0038 0.0001 0.0001 0.0006 0.0035 0.0001 0.0049 0 0.0002 0 0.0001 0.0004 0.001 0 0.0006 0.001 0.0059 0.0002
Vacuole Periphery 0.001 0.0001 0.0001 0.0006 0.0029 0 0.0018 0 0.0003 0 0.0001 0.0002 0.0012 0 0.0004 0.0006 0.0017 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 35.0287 36.5325 21.2997 43.796 28.3852 46.6791 32.9803 22.2595 43.2237 39.5739
Translational Efficiency 1.9445 1.9716 3.2939 2.384 2.2812 1.8702 2.1474 4.2725 2.1118 2.4228

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Non-essential INO80 chromatin remodeling complex subunit; deletion affects telomere maintenance via recombination
Localization
Cell Percentages nucleus (100%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Ies5

Ies5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ies5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available