Standard name
Human Ortholog
Description 14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of exocytosis, vesicle transport, Ras/MAPK and rapamycin-sensitive signaling, aggresome formation, spindle position checkpoint; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress; antiapoptotic gene similar to human 14-3-3; BMH1 has a paralog, BMH2, that arose from whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0.05 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.99 0.98 0.96 0.99 0.95 0.92 0.98 0.94 0.94 0.93 0.99 0.99 0.99 0.99 0.99 1.0 0.98 0.95 0.96 0.94 0.93 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0 0.07 0 0 0 0.05 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 0 0 0 2 0 0 0 0 0 0 2 0 0 0 1 0 1 0 1 2 3 3 6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 0 2 0 3 5 6 9 5 9 7 9 3 0 1 6 5 2 0 0 0 0 0 0
Cytoplasm 343 138 217 164 136 181 275 260 215 183 143 158 275 374 338 360 203 211 332 125 196 230 307 301
Endoplasmic Reticulum 1 1 2 4 4 1 13 20 2 6 3 8 4 1 3 17 6 9 0 0 1 0 0 2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 1 0 0 0 0 0 0 0 1 0 0 1 0 2 1 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0
Nucleus 0 0 0 0 1 0 0 1 0 1 0 0 0 0 0 2 0 0 0 3 0 3 8 13
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 0 0 0 1 0 0 1 0 1 0 1 4 0 1 1 1 0 0 1 2 6
Unique Cell Count 344 139 220 168 141 183 291 284 220 194 152 170 277 377 342 365 206 212 340 132 205 245 332 346
Labelled Cell Count 347 139 223 168 146 187 295 290 224 200 156 178 282 377 346 388 217 224 340 132 205 245 332 346


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 128.8 89.7 104.0 103.3 81.2 97.4 94.4 89.1 86.6 90.7 93.4 89.4 112.8 109.8 105.6 83.3 107.2 113.1 107.8 110.4 115.4
Std Deviation (1e-4) 20.0 22.0 26.8 32.6 25.2 28.8 24.4 23.8 26.1 30.3 30.8 29.6 29.7 24.4 29.6 21.7 29.3 33.1 30.7 31.7 37.1
Intensity Change (Log2) – – – -0.01 -0.36 -0.09 -0.14 -0.22 -0.26 -0.2 -0.16 -0.22 0.12 0.08 0.02 -0.32 0.04 0.12 0.05 0.09 0.15


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 2.4 0 0 0 0 0 0 0 0
Cytoplasm -0.7 -1.4 0.2 -2.4 -3.5 -0.7 -2.4 -2.4 -2.9 0.7 0.7 0.2 0 -0.1 1.0
Endoplasmic Reticulum 0 0 0 2.4 3.3 0 0 0 0 0 0 0 2.5 0 2.2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 138.9407 153.6366 135.183 102.3861 124.5096 145.332 156.9084 171.6184 159.6103 160.8865 133.623 163.4205 118.8375 147.3605 139.8982 129.7134 112.8217 127.3099
Actin 0.0125 0.0001 0.0053 0.0214 0.024 0.0005 0.0059 0.0002 0.0002 0 0.0002 0.0001 0.0015 0.0002 0.0001 0.0012 0.0323 0.0032
Bud 0.0006 0 0.0005 0.0046 0.0011 0.0009 0.0003 0 0.0005 0 0.0017 0.0004 0.0002 0 0.0009 0.0003 0.0033 0.0019
Bud Neck 0.0006 0.0001 0.0012 0.0011 0.0016 0.002 0.0002 0.0001 0.0002 0.0001 0.0003 0.0004 0.0004 0.0001 0.0002 0.0003 0.0002 0.002
Bud Periphery 0.0004 0 0.0004 0.0057 0.0011 0.0005 0.0002 0 0.0002 0 0.0009 0.0001 0.0001 0 0.0003 0.0001 0.0054 0.0006
Bud Site 0.0007 0 0.0013 0.0027 0.0018 0.0002 0.0002 0 0.0001 0 0.0005 0 0.0002 0 0.0001 0.0001 0.0003 0.0004
Cell Periphery 0.0006 0.0001 0.0003 0.0006 0.0002 0.0005 0.0003 0 0.0003 0 0.001 0.0001 0.0002 0 0 0 0.0002 0.0001
Cytoplasm 0.9322 0.9945 0.923 0.7726 0.8987 0.95 0.9712 0.9986 0.9722 0.9984 0.8782 0.9891 0.8936 0.9987 0.9906 0.9639 0.8007 0.924
Cytoplasmic Foci 0.0106 0.0001 0.0095 0.0543 0.0042 0.002 0.0025 0.0002 0.005 0.0001 0.0199 0.0004 0.0004 0.0001 0.0011 0.0007 0.0004 0.0019
Eisosomes 0.0003 0 0.0002 0.0002 0.0002 0 0.0002 0 0.0001 0 0.0004 0 0 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0015 0.0001 0.0005 0.0014 0.0012 0.0005 0.0007 0.0002 0.0001 0 0.0005 0.0002 0.004 0 0.0001 0.0049 0.0022 0.0019
Endosome 0.0048 0 0.0134 0.0107 0.0191 0.001 0.0015 0 0.0001 0 0.0006 0.0001 0.0011 0 0.0003 0.0055 0.0072 0.0025
Golgi 0.0016 0 0.0054 0.0092 0.0153 0.0001 0.0005 0 0 0 0.0001 0 0.0001 0 0 0.0002 0.0017 0.0001
Lipid Particles 0.0032 0 0.0049 0.0233 0.0053 0.0001 0.0005 0 0.0008 0 0.0034 0 0 0 0 0 0.0002 0
Mitochondria 0.0019 0 0.0041 0.054 0.0083 0.0009 0.0014 0 0.0022 0 0.0092 0.0001 0.0007 0 0.0009 0.0008 0.0846 0.0008
None 0.0085 0.0002 0.006 0.0061 0.0007 0.0034 0.0042 0.0001 0.0081 0.0002 0.0136 0.0017 0.0007 0.0001 0.0017 0.0003 0.0013 0.0022
Nuclear Periphery 0.0046 0.0027 0.0017 0.0017 0.0051 0.0018 0.0026 0.0001 0.0004 0.0002 0.0041 0.0006 0.0518 0.0001 0.0002 0.0134 0.035 0.0207
Nucleolus 0.0022 0 0.0043 0.0045 0.0006 0.0023 0.0019 0 0.0036 0 0.028 0.0005 0.0001 0 0.0001 0 0.0004 0.0003
Nucleus 0.0052 0.0017 0.005 0.006 0.0043 0.0127 0.0028 0.0004 0.0023 0.0009 0.0172 0.0042 0.0419 0.0006 0.0012 0.0058 0.0109 0.0334
Peroxisomes 0.0015 0 0.0031 0.004 0.0028 0.0001 0.0002 0 0.0002 0 0.0006 0 0 0 0.0001 0 0.0003 0.0001
Punctate Nuclear 0.0022 0 0.0043 0.0029 0.0012 0.0003 0.0003 0 0.0003 0 0.001 0.0002 0.0005 0 0 0.0002 0.0003 0.0028
Vacuole 0.0032 0.0001 0.0026 0.0061 0.002 0.0168 0.0015 0 0.0018 0 0.0088 0.0015 0.0013 0 0.0016 0.0013 0.0025 0.0007
Vacuole Periphery 0.0012 0 0.0029 0.0068 0.0013 0.0035 0.0008 0 0.0014 0 0.0099 0.0003 0.001 0 0.0006 0.0011 0.0104 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 579.8363 563.1177 678.2221 708.7137 702.8505 621.3501 753.575 739.267 875.7942 674.6915
Translational Efficiency 1.7955 2.0241 1.8433 2.1567 2.1094 1.9553 1.7193 2.1039 1.6527 1.9371

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description 14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of exocytosis, vesicle transport, Ras/MAPK and rapamycin-sensitive signaling, aggresome formation, spindle position checkpoint; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress; antiapoptotic gene similar to human 14-3-3; BMH1 has a paralog, BMH2, that arose from whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Bmh1

Bmh1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bmh1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available