Standard name | |
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Human Ortholog | |
Description | Putative protein of unknown function; mutant has long chronological lifespan; has homology to Staphylococcus aureus nuclease; GFP-fusion protein localizes to mitochondria; is induced in response to the DNA-damaging agent MMS |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 4 | 1 | – | 0 | 5 | 5 | 3 | 0 | 6 | 12 | 15 |
Bud | 2 | 1 | 0 | 2 | 1 | 0 | 2 | 4 | 11 | 2 | 6 | 5 | 1 | 5 | 0 | – | 5 | 4 | 1 | 0 | 0 | 2 | 7 | 7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 1 | 2 | 1 | 2 | 0 | – | 3 | 2 | 2 | 0 | 0 | 1 | 5 | 4 |
Bud Site | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 4 | 1 | 3 | 4 | 0 | 0 | 0 | – | 0 | 3 | – | – | – | – | – | – |
Cell Periphery | 2 | 4 | 0 | 2 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 9 | 8 | 4 | – | 8 | 3 | 0 | 0 | 0 | 1 | 2 | 0 |
Cytoplasm | 5 | 32 | 3 | 14 | 2 | 8 | 17 | 26 | 12 | 12 | 14 | 16 | 101 | 188 | 207 | – | 85 | 84 | 1 | 5 | 0 | 5 | 10 | 15 |
Endoplasmic Reticulum | 0 | 3 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 2 | 0 | 1 | 8 | 9 | 8 | – | 21 | 29 | 3 | 2 | 0 | 2 | 6 | 6 |
Endosome | 49 | 14 | 4 | 3 | 0 | 0 | 7 | 15 | 0 | 0 | 0 | 0 | 40 | 63 | 57 | – | 59 | 21 | 41 | 12 | 3 | 19 | 34 | 30 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 3 | 2 | – | 6 | 1 | 16 | 5 | 0 | 6 | 16 | 10 |
Mitochondria | 112 | 92 | 7 | 45 | 14 | 45 | 42 | 69 | 135 | 119 | 126 | 126 | 27 | 31 | 44 | – | 32 | 28 | 172 | 139 | 44 | 200 | 379 | 303 |
Nucleus | 3 | 2 | 0 | 0 | 0 | 1 | 1 | 5 | 1 | 2 | 2 | 3 | 0 | 0 | 1 | – | 4 | 8 | 1 | 2 | 0 | 1 | 3 | 1 |
Nuclear Periphery | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 4 | 0 | 0 | 1 | 1 | 6 |
Nucleolus | 11 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 4 | 0 | 0 | 2 | 1 | 0 |
Peroxisomes | 18 | 8 | 2 | 3 | 0 | 0 | 0 | 2 | 0 | 1 | 2 | 0 | 23 | 54 | 48 | – | 61 | 7 | 4 | 3 | 3 | 7 | 22 | 19 |
SpindlePole | 6 | 3 | 2 | 1 | 1 | 4 | 4 | 16 | 12 | 9 | 8 | 9 | 12 | 25 | 13 | – | 53 | 52 | 3 | 3 | 0 | 4 | 8 | 5 |
Vac/Vac Membrane | 20 | 15 | 1 | 2 | 0 | 1 | 5 | 9 | 4 | 4 | 2 | 5 | 17 | 24 | 28 | – | 40 | 33 | 31 | 7 | 0 | 13 | 18 | 17 |
Unique Cell Count | 179 | 145 | 16 | 62 | 16 | 55 | 69 | 124 | 145 | 136 | 140 | 143 | 207 | 369 | 370 | 303 | 238 | 299 | 191 | 56 | 278 | 536 | 455 | |
Labelled Cell Count | 231 | 178 | 21 | 74 | 19 | 61 | 79 | 153 | 183 | 157 | 166 | 171 | 242 | 417 | 413 | 377 | 280 | 299 | 191 | 56 | 278 | 536 | 455 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.6 | 4.5 | 5.6 | 4.9 | 3.7 | 4.4 | 4.8 | 5.2 | 3.7 | 3.8 | 3.6 | 3.7 | 5.7 | 5.5 | 5.5 | 4.5 | 5.5 | 5.4 | 5.8 | 6.3 | 6.9 |
Std Deviation (1e-4) | 0.6 | 3.0 | 0.8 | 1.8 | 0.5 | 1.9 | 1.8 | 2.3 | 1.5 | 1.1 | 0.9 | 1.0 | 1.3 | 1.4 | 1.4 | 1.0 | 1.5 | 1.2 | 1.3 | 2.0 | 2.2 |
Intensity Change (Log2) | – | – | – | -0.18 | -0.61 | -0.35 | -0.23 | -0.11 | -0.61 | -0.55 | -0.63 | -0.58 | 0.02 | -0.03 | -0.04 | -0.31 | -0.01 | -0.05 | 0.06 | 0.18 | 0.29 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.0457 | 0.0327 | 0.0325 | -0.4428 | -0.1891 | 0.281 | 5.2094 | 5.2833 | 5.313 | 4.6174 | 4.6448 | 5.4299 | 2.1958 | 2.5149 | 2.3835 | 2.2566 | 2.0415 | 2.5638 |
Actin | 0.0428 | 0.0079 | 0.0548 | 0.0084 | 0.0124 | 0.01 | 0.025 | 0.0167 | 0.0257 | 0.0094 | 0.0339 | 0.0178 | 0.0192 | 0.006 | 0.0131 | 0.0297 | 0.0078 | 0.009 |
Bud | 0.0028 | 0.0024 | 0.0038 | 0.0048 | 0.0019 | 0.0019 | 0.0016 | 0.0047 | 0.004 | 0.0022 | 0.0063 | 0.0016 | 0.002 | 0.0047 | 0.0032 | 0.0027 | 0.0104 | 0.0018 |
Bud Neck | 0.0051 | 0.0013 | 0.0038 | 0.0012 | 0.0019 | 0.0144 | 0.0018 | 0.0031 | 0.0068 | 0.0019 | 0.0044 | 0.0091 | 0.0139 | 0.0058 | 0.0073 | 0.0019 | 0.0014 | 0.0102 |
Bud Periphery | 0.0071 | 0.0053 | 0.0098 | 0.0212 | 0.0061 | 0.0065 | 0.0039 | 0.0076 | 0.0121 | 0.0069 | 0.0224 | 0.0045 | 0.0046 | 0.0098 | 0.0079 | 0.0059 | 0.0526 | 0.0045 |
Bud Site | 0.0171 | 0.0255 | 0.0258 | 0.0043 | 0.0017 | 0.0015 | 0.0064 | 0.0544 | 0.0249 | 0.0079 | 0.0072 | 0.0021 | 0.0116 | 0.0593 | 0.0186 | 0.0073 | 0.0029 | 0.0024 |
Cell Periphery | 0.0025 | 0.0018 | 0.0033 | 0.0012 | 0.0008 | 0.0012 | 0.0025 | 0.0026 | 0.0049 | 0.0013 | 0.0018 | 0.0011 | 0.0018 | 0.0027 | 0.0026 | 0.0007 | 0.0012 | 0.0018 |
Cytoplasm | 0.0657 | 0.1236 | 0.0773 | 0.0718 | 0.0798 | 0.0888 | 0.0601 | 0.0864 | 0.0943 | 0.0647 | 0.0945 | 0.0852 | 0.0824 | 0.0891 | 0.0662 | 0.0959 | 0.0899 | 0.0754 |
Cytoplasmic Foci | 0.1082 | 0.1185 | 0.0971 | 0.1256 | 0.1706 | 0.1312 | 0.1719 | 0.1443 | 0.1254 | 0.1419 | 0.1516 | 0.1737 | 0.1523 | 0.1254 | 0.1254 | 0.1477 | 0.15 | 0.1457 |
Eisosomes | 0.0023 | 0.0007 | 0.0026 | 0.0006 | 0.0017 | 0.0004 | 0.0019 | 0.0007 | 0.0016 | 0.0007 | 0.0042 | 0.0012 | 0.0012 | 0.0006 | 0.002 | 0.0005 | 0.0003 | 0.0009 |
Endoplasmic Reticulum | 0.0098 | 0.0146 | 0.0062 | 0.0025 | 0.0025 | 0.0085 | 0.0049 | 0.0027 | 0.0047 | 0.0027 | 0.0046 | 0.0031 | 0.0072 | 0.0056 | 0.0066 | 0.0044 | 0.0031 | 0.0039 |
Endosome | 0.1587 | 0.1717 | 0.1133 | 0.081 | 0.1795 | 0.1282 | 0.1528 | 0.1582 | 0.1317 | 0.1653 | 0.2591 | 0.1258 | 0.1746 | 0.1679 | 0.0931 | 0.1701 | 0.1383 | 0.1106 |
Golgi | 0.0472 | 0.0476 | 0.0432 | 0.038 | 0.0592 | 0.0469 | 0.0553 | 0.0783 | 0.048 | 0.0662 | 0.1069 | 0.0524 | 0.0466 | 0.0529 | 0.05 | 0.0567 | 0.0385 | 0.0363 |
Lipid Particles | 0.0603 | 0.0188 | 0.0232 | 0.0105 | 0.0379 | 0.0398 | 0.0347 | 0.0187 | 0.0194 | 0.0049 | 0.0059 | 0.0156 | 0.0444 | 0.0154 | 0.0164 | 0.0053 | 0.0234 | 0.0327 |
Mitochondria | 0.1822 | 0.2289 | 0.2082 | 0.331 | 0.2829 | 0.2568 | 0.239 | 0.2361 | 0.3035 | 0.3002 | 0.1797 | 0.2908 | 0.1627 | 0.1586 | 0.3128 | 0.2769 | 0.2186 | 0.2685 |
None | 0.2121 | 0.1795 | 0.2618 | 0.2058 | 0.0615 | 0.1659 | 0.1361 | 0.1302 | 0.1358 | 0.1542 | 0.0552 | 0.1362 | 0.2226 | 0.2389 | 0.2177 | 0.1506 | 0.193 | 0.2168 |
Nuclear Periphery | 0.009 | 0.003 | 0.0031 | 0.0007 | 0.0065 | 0.0145 | 0.0172 | 0.0008 | 0.001 | 0.0007 | 0.0036 | 0.0009 | 0.0021 | 0.002 | 0.0074 | 0.0046 | 0.0008 | 0.0016 |
Nucleolus | 0.0031 | 0.0013 | 0.0015 | 0.001 | 0.001 | 0.0008 | 0.001 | 0.0008 | 0.0015 | 0.0006 | 0.0004 | 0.0008 | 0.0025 | 0.0016 | 0.0015 | 0.001 | 0.0007 | 0.0014 |
Nucleus | 0.0054 | 0.006 | 0.0021 | 0.0013 | 0.0025 | 0.0069 | 0.0142 | 0.0011 | 0.0016 | 0.0008 | 0.0016 | 0.001 | 0.0023 | 0.0025 | 0.0033 | 0.0017 | 0.001 | 0.0074 |
Peroxisomes | 0.0324 | 0.0171 | 0.0371 | 0.0744 | 0.0412 | 0.06 | 0.0426 | 0.041 | 0.0366 | 0.0575 | 0.0387 | 0.065 | 0.0267 | 0.0278 | 0.0295 | 0.0212 | 0.0516 | 0.0521 |
Punctate Nuclear | 0.0033 | 0.0017 | 0.0027 | 0.0019 | 0.0274 | 0.0027 | 0.0165 | 0.0009 | 0.0019 | 0.0006 | 0.0036 | 0.0015 | 0.0032 | 0.0019 | 0.0053 | 0.0017 | 0.0028 | 0.0045 |
Vacuole | 0.0165 | 0.0176 | 0.0142 | 0.0074 | 0.0159 | 0.0085 | 0.0074 | 0.0078 | 0.0104 | 0.0056 | 0.0092 | 0.0073 | 0.012 | 0.016 | 0.0074 | 0.008 | 0.0081 | 0.0086 |
Vacuole Periphery | 0.0062 | 0.0052 | 0.0051 | 0.0054 | 0.0051 | 0.0047 | 0.0034 | 0.0029 | 0.0038 | 0.0039 | 0.0051 | 0.0033 | 0.0039 | 0.0054 | 0.0026 | 0.0055 | 0.0037 | 0.0036 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 7.356 | 8.3693 | 3.5488 | 8.5914 | 5.2022 | 9.357 | 8.7644 | 12.6466 | 10.0086 | 8.3905 |
Translational Efficiency | 0.73 | 0.5308 | 1.1009 | 0.6471 | 0.8587 | 0.5943 | 0.647 | 0.3834 | 0.4615 | 0.552 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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