Standard name | |
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Human Ortholog | |
Description | Vacuolar alpha mannosidase; involved in free oligosaccharide (fOS) degradation; delivered to the vacuole in a novel pathway separate from the secretory pathway |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 1 | 0 | 1 | 1 | 2 | 1 | – | – | 1 | 2 | 4 | 6 | 5 | 6 | 4 |
Bud | 1 | 3 | 1 | 4 | 14 | 22 | 20 | 22 | – | 9 | 13 | 14 | 0 | 0 | 0 | – | – | 4 | 7 | 4 | 3 | 5 | 7 | 12 |
Bud Neck | 2 | 0 | 1 | 0 | 4 | 1 | 0 | 0 | – | 1 | 0 | 0 | 2 | 1 | 0 | – | – | 0 | 1 | 2 | 2 | 0 | 4 | 3 |
Bud Site | 0 | 0 | 1 | 0 | 2 | 5 | 2 | 3 | – | 3 | 3 | 2 | 0 | 0 | 0 | – | – | 2 | – | – | – | – | – | – |
Cell Periphery | 2 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | – | 1 | 2 | 0 | 0 | 0 | 0 | – | – | 0 | 2 | 0 | 2 | 3 | 2 | 2 |
Cytoplasm | 394 | 318 | 322 | 235 | 87 | 124 | 201 | 216 | – | 215 | 168 | 263 | 220 | 220 | 205 | – | – | 230 | 336 | 283 | 216 | 186 | 255 | 223 |
Endoplasmic Reticulum | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 6 | 6 | 3 | – | – | 10 | 0 | 0 | 5 | 1 | 2 | 1 |
Endosome | 1 | 2 | 4 | 4 | 102 | 121 | 129 | 73 | – | 48 | 30 | 54 | 2 | 2 | 2 | – | – | 58 | 10 | 5 | 14 | 4 | 5 | 3 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 1 | 2 | 1 | 2 | 0 | 0 | 2 |
Mitochondria | 7 | 18 | 13 | 20 | 4 | 29 | 18 | 35 | – | 55 | 26 | 18 | 0 | 0 | 0 | – | – | 13 | 11 | 12 | 44 | 32 | 32 | 22 |
Nucleus | 0 | 0 | 0 | 0 | 2 | 7 | 8 | 29 | – | 29 | 19 | 7 | 2 | 2 | 2 | – | – | 4 | 0 | 1 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 2 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Nucleolus | 0 | 0 | 1 | 0 | 12 | 50 | 69 | 47 | – | 29 | 23 | 28 | 0 | 0 | 0 | – | – | 18 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1 | 0 | 2 | 0 | 29 | 14 | 6 | 3 | – | 4 | 4 | 4 | 0 | 0 | 0 | – | – | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 7 | 1 | 8 | 5 | 110 | 93 | 92 | 53 | – | 31 | 33 | 34 | 2 | 1 | 4 | – | – | 61 | 14 | 6 | 14 | 5 | 6 | 14 |
Vac/Vac Membrane | 1 | 2 | 5 | 0 | 26 | 102 | 205 | 221 | – | 201 | 128 | 272 | 0 | 0 | 0 | – | – | 101 | 9 | 7 | 15 | 7 | 7 | 12 |
Unique Cell Count | 410 | 331 | 336 | 246 | 269 | 398 | 543 | 537 | 484 | 340 | 559 | 232 | 230 | 215 | 377 | 408 | 338 | 335 | 262 | 339 | 312 | |||
Labelled Cell Count | 417 | 347 | 361 | 268 | 393 | 569 | 750 | 702 | 629 | 450 | 699 | 235 | 234 | 217 | 508 | 408 | 338 | 335 | 262 | 339 | 312 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.7 | 3.7 | 3.5 | 3.3 | 4.8 | 5.4 | 6.1 | 6.2 | – | 6.1 | 6.5 | 7.5 | 5.1 | 5.1 | 5.2 | 4.7 | 4.7 | 6.6 | 5.0 | 5.1 | 5.6 |
Std Deviation (1e-4) | 0.6 | 0.9 | 1.6 | 1.3 | 1.8 | 1.7 | 1.9 | 2.0 | – | 2.1 | 2.4 | 2.9 | 1.2 | 1.2 | 1.1 | 1.7 | 1.2 | 2.8 | 1.9 | 1.6 | 2.2 |
Intensity Change (Log2) | – | – | – | -0.08 | 0.46 | 0.63 | 0.8 | 0.83 | – | 0.79 | 0.89 | 1.1 | 0.55 | 0.55 | 0.58 | 0.44 | 0.42 | 0.93 | 0.52 | 0.54 | 0.68 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 9.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0269 | 0.0058 | 0.0097 | 0.0032 | 0.0048 | 0.0023 | 0.032 | 0.0001 | 0.0301 | 0.0343 | 0.0241 | 0.0081 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0011 |
Bud | 0.0024 | 0.0068 | 0.0016 | 0.0005 | 0.0005 | 0.0006 | 0.0023 | 0.0032 | 0.0025 | 0.0002 | 0.0014 | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0.0001 |
Bud Neck | 0.0095 | 0.0039 | 0.0017 | 0.0007 | 0.0003 | 0.0011 | 0.0026 | 0.0001 | 0.0009 | 0.0152 | 0.0006 | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0.0001 |
Bud Periphery | 0.0055 | 0.01 | 0.0018 | 0.0008 | 0.0012 | 0.0013 | 0.0096 | 0.0091 | 0.0096 | 0.0002 | 0.0035 | 0.001 | 0 | 0 | 0 | 0 | 0 | 0.0001 |
Bud Site | 0.0087 | 0.0246 | 0.0086 | 0.0006 | 0.0022 | 0.0005 | 0.0062 | 0.0016 | 0.01 | 0.0016 | 0.0025 | 0.0008 | 0 | 0 | 0 | 0 | 0 | 0.0003 |
Cell Periphery | 0.0014 | 0.0011 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0005 | 0.0003 | 0.0005 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.0431 | 0.0922 | 0.0648 | 0.0612 | 0.0454 | 0.0973 | 0.0885 | 0.0772 | 0.0526 | 0.0625 | 0.0879 | 0.1523 | 0.0281 | 0.0957 | 0.0703 | 0.2525 | 0.0051 | 0.0553 |
Cytoplasmic Foci | 0.0451 | 0.0305 | 0.0201 | 0.0849 | 0.0728 | 0.0386 | 0.0874 | 0.0027 | 0.0646 | 0.048 | 0.0121 | 0.03 | 0.0007 | 0.0164 | 0.0009 | 0.0046 | 0.0004 | 0.0655 |
Eisosomes | 0.0003 | 0.0002 | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0.0006 | 0.0003 | 0.0004 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0044 | 0.0015 | 0.0016 | 0.0011 | 0.0024 | 0.0021 | 0.0085 | 0.0005 | 0.0028 | 0.0003 | 0.0009 | 0.0017 | 0.0008 | 0.0021 | 0.0005 | 0.0011 | 0 | 0.0004 |
Endosome | 0.0305 | 0.0154 | 0.0113 | 0.0194 | 0.0219 | 0.0133 | 0.0315 | 0.0005 | 0.0213 | 0.0015 | 0.0127 | 0.0081 | 0.0001 | 0.0108 | 0.0001 | 0.0042 | 0 | 0.0154 |
Golgi | 0.0088 | 0.0012 | 0.0035 | 0.0073 | 0.0029 | 0.0045 | 0.0082 | 0.0001 | 0.0073 | 0.0047 | 0.0168 | 0.0018 | 0 | 0.0002 | 0 | 0 | 0 | 0.009 |
Lipid Particles | 0.0177 | 0.0041 | 0.0062 | 0.0098 | 0.0134 | 0.0052 | 0.0108 | 0.0002 | 0.0136 | 0.0024 | 0.0029 | 0.0126 | 0.0001 | 0.0009 | 0.0001 | 0 | 0 | 0.0046 |
Mitochondria | 0.0073 | 0.0028 | 0.0077 | 0.0068 | 0.0135 | 0.008 | 0.0046 | 0.0014 | 0.0192 | 0.0003 | 0.0081 | 0.0045 | 0 | 0.0006 | 0 | 0.0001 | 0 | 0.003 |
None | 0.7255 | 0.7533 | 0.8278 | 0.7683 | 0.7157 | 0.7974 | 0.6693 | 0.8991 | 0.7345 | 0.7971 | 0.7857 | 0.7363 | 0.9693 | 0.8652 | 0.9273 | 0.7338 | 0.9943 | 0.8352 |
Nuclear Periphery | 0.022 | 0.0025 | 0.0016 | 0.0018 | 0.0417 | 0.0023 | 0.0097 | 0.0003 | 0.0052 | 0.0001 | 0.0097 | 0.0041 | 0.0001 | 0.0008 | 0.0001 | 0.0005 | 0 | 0.0002 |
Nucleolus | 0.0023 | 0.0022 | 0.0007 | 0.0005 | 0.001 | 0.0006 | 0.0007 | 0.0005 | 0.0013 | 0.0001 | 0.0068 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 | 0 |
Nucleus | 0.0123 | 0.0043 | 0.0083 | 0.001 | 0.0272 | 0.0034 | 0.0061 | 0.001 | 0.0013 | 0.0002 | 0.0108 | 0.0037 | 0.0001 | 0.0006 | 0.0001 | 0.0004 | 0 | 0.0001 |
Peroxisomes | 0.0071 | 0.0115 | 0.0026 | 0.0162 | 0.0079 | 0.0066 | 0.0108 | 0.0002 | 0.0151 | 0.0047 | 0.0049 | 0.0203 | 0 | 0.0001 | 0 | 0 | 0 | 0.008 |
Punctate Nuclear | 0.0072 | 0.0073 | 0.0125 | 0.0122 | 0.0025 | 0.0097 | 0.004 | 0.0006 | 0.0024 | 0.0261 | 0.005 | 0.0019 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0 | 0.0014 |
Vacuole | 0.0089 | 0.0163 | 0.0066 | 0.0028 | 0.0202 | 0.0045 | 0.0049 | 0.001 | 0.0035 | 0.0002 | 0.002 | 0.0109 | 0.0001 | 0.0051 | 0.0001 | 0.002 | 0 | 0.0002 |
Vacuole Periphery | 0.0033 | 0.0025 | 0.0007 | 0.0007 | 0.002 | 0.0008 | 0.0012 | 0.0002 | 0.0012 | 0 | 0.0014 | 0.0008 | 0 | 0.0006 | 0 | 0.0005 | 0 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 8.1432 | 4.853 | 2.9259 | 5.0297 | 3.2994 | 7.7996 | 7.8203 | 10.6599 | 11.1828 | 11.4006 |
Translational Efficiency | 0.6936 | 0.6445 | 1.0037 | 0.687 | 0.9677 | 1.1041 | 0.6441 | 0.3405 | 0.6574 | 0.6108 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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