Standard name | |
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Human Ortholog | |
Description | NADPH-dependent aldehyde reductase; utilizes aromatic and alophatic aldehyde substrates; member of the short-chain dehydrogenase/reductase superfamily |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 |
Bud | – | – | 0 | 0 | 0 | 1 | 2 | 3 | 0 | 4 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 |
Bud Neck | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Bud Site | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | – | – | 1 | 1 | 7 | 2 | 4 | 5 | 3 | 3 | 5 | 10 | 4 | 1 | 3 | 46 | 35 | 37 | – | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | – | – | 27 | 78 | 123 | 115 | 145 | 114 | 110 | 130 | 95 | 112 | 43 | 87 | 77 | 497 | 489 | 488 | – | 29 | 61 | 52 | 102 | 115 |
Endoplasmic Reticulum | – | – | 0 | 1 | 0 | 1 | 0 | 0 | 3 | 1 | 0 | 3 | 0 | 0 | 0 | 14 | 15 | 13 | – | 0 | 0 | 0 | 1 | 2 |
Endosome | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | – | 0 | 0 | 0 | 1 | 0 |
Golgi | – | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 2 | – | 0 | 0 | 0 | 1 | 2 |
Mitochondria | – | – | 0 | 3 | 6 | 23 | 47 | 38 | 62 | 51 | 82 | 44 | 0 | 0 | 0 | 2 | 6 | 5 | – | 0 | 0 | 1 | 6 | 5 |
Nucleus | – | – | 1 | 0 | 0 | 3 | 4 | 7 | 5 | 7 | 6 | 6 | 0 | 1 | 0 | 4 | 0 | 6 | – | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Nucleolus | – | – | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
SpindlePole | – | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | – | – | 2 | 14 | 19 | 15 | 28 | 15 | 12 | 26 | 25 | 24 | 0 | 6 | 6 | 30 | 80 | 36 | – | 0 | 0 | 0 | 1 | 2 |
Unique Cell Count | 30 | 95 | 146 | 146 | 200 | 160 | 172 | 199 | 166 | 177 | 44 | 92 | 81 | 536 | 559 | 532 | 34 | 66 | 57 | 121 | 138 | |||
Labelled Cell Count | 31 | 98 | 155 | 160 | 231 | 183 | 198 | 223 | 217 | 201 | 47 | 95 | 86 | 597 | 629 | 588 | 34 | 66 | 57 | 121 | 138 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | – | 8.0 | 7.4 | 7.9 | 6.5 | 6.4 | 6.8 | 5.6 | 6.3 | 5.8 | 6.4 | 8.8 | 9.8 | 9.8 | 8.7 | 8.3 | 8.2 | 8.8 | 8.1 | 8.8 |
Std Deviation (1e-4) | – | – | 1.1 | 1.7 | 2.4 | 1.6 | 1.6 | 2.0 | 1.4 | 1.6 | 1.5 | 1.5 | 1.3 | 1.9 | 2.0 | 1.1 | 1.3 | 1.2 | 3.1 | 1.7 | 2.6 |
Intensity Change (Log2) | – | – | – | -0.1 | -0.02 | -0.3 | -0.32 | -0.24 | -0.52 | -0.34 | -0.45 | -0.31 | 0.14 | 0.29 | 0.29 | 0.12 | 0.06 | 0.04 | 0.14 | 0.02 | 0.13 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.0 | -0.8 | -1.4 | -2.1 | -2.2 | -2.8 | -2.7 | -3.4 | -2.9 | 1.4 | 0.9 | 1.0 | 0.6 | -0.4 | 0.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 3.0 | 3.0 | 4.0 | 3.1 | 5.0 | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.5112 | 0.0189 | 0.0033 | 0.0003 | 0.0001 | 0.001 | 0.1643 | 0.0013 | 0.0003 | 0.0004 | 0.0003 | 0.0002 | – | – | – | – | – | – |
Bud | 0.003 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0023 | 0.0004 | 0.0003 | 0.0003 | 0.0004 | 0.0003 | – | – | – | – | – | – |
Bud Neck | 0.2775 | 0.0002 | 0.0004 | 0.0008 | 0.0002 | 0.0011 | 0.009 | 0.0004 | 0.0007 | 0.0013 | 0.0018 | 0.002 | – | – | – | – | – | – |
Bud Periphery | 0.0038 | 0.0001 | 0 | 0 | 0 | 0 | 0.0022 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | – | – | – | – | – | – |
Bud Site | 0.026 | 0.0013 | 0.0003 | 0 | 0 | 0.0001 | 0.0051 | 0.0014 | 0.0005 | 0.0003 | 0.0005 | 0.0002 | – | – | – | – | – | – |
Cell Periphery | 0.0029 | 0.0001 | 0 | 0 | 0 | 0 | 0.0006 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cytoplasm | 0.0049 | 0.9495 | 0.9073 | 0.9859 | 0.994 | 0.9146 | 0.4416 | 0.9446 | 0.8926 | 0.88 | 0.8875 | 0.8711 | – | – | – | – | – | – |
Cytoplasmic Foci | 0.0195 | 0.0035 | 0.0106 | 0.0018 | 0.0032 | 0.0065 | 0.0517 | 0.0056 | 0.0069 | 0.0064 | 0.0044 | 0.0066 | – | – | – | – | – | – |
Eisosomes | 0.0036 | 0.0002 | 0 | 0 | 0 | 0 | 0.0019 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Endoplasmic Reticulum | 0.0115 | 0.0016 | 0.0016 | 0.0006 | 0.0001 | 0.0032 | 0.0196 | 0.0013 | 0.002 | 0.0023 | 0.002 | 0.0024 | – | – | – | – | – | – |
Endosome | 0.0288 | 0.0012 | 0.0158 | 0.0003 | 0.0011 | 0.0071 | 0.125 | 0.002 | 0.005 | 0.0049 | 0.0034 | 0.0081 | – | – | – | – | – | – |
Golgi | 0.0519 | 0.003 | 0.0458 | 0 | 0.0001 | 0.0005 | 0.0229 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | – | – | – | – | – | – |
Lipid Particles | 0.0181 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0184 | 0.0003 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Mitochondria | 0.013 | 0.0018 | 0.001 | 0.0001 | 0.0001 | 0.0004 | 0.0131 | 0.0011 | 0.0004 | 0.0004 | 0.0003 | 0.001 | – | – | – | – | – | – |
None | 0.0013 | 0.0104 | 0.0029 | 0.0028 | 0.0007 | 0.0067 | 0.0408 | 0.0086 | 0.006 | 0.0068 | 0.0055 | 0.0061 | – | – | – | – | – | – |
Nuclear Periphery | 0.0028 | 0.0011 | 0.001 | 0.0007 | 0 | 0.003 | 0.0308 | 0.0021 | 0.0048 | 0.0069 | 0.0042 | 0.0051 | – | – | – | – | – | – |
Nucleolus | 0.0007 | 0 | 0 | 0 | 0 | 0 | 0.002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | – | – | – | – | – | – |
Nucleus | 0.0008 | 0.0053 | 0.0087 | 0.0055 | 0.0001 | 0.0529 | 0.0155 | 0.026 | 0.0751 | 0.0825 | 0.084 | 0.0902 | – | – | – | – | – | – |
Peroxisomes | 0.0141 | 0.0003 | 0.0001 | 0 | 0 | 0.0001 | 0.0096 | 0.001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | – | – | – | – | – | – |
Punctate Nuclear | 0.0017 | 0.0005 | 0.0005 | 0.0007 | 0 | 0.0007 | 0.0141 | 0.0028 | 0.0031 | 0.0049 | 0.0037 | 0.0033 | – | – | – | – | – | – |
Vacuole | 0.0018 | 0.0004 | 0.0003 | 0.0002 | 0.0001 | 0.0015 | 0.0054 | 0.0005 | 0.0014 | 0.0016 | 0.0016 | 0.0024 | – | – | – | – | – | – |
Vacuole Periphery | 0.001 | 0.0001 | 0.0001 | 0 | 0 | 0.0003 | 0.0043 | 0.0001 | 0.0003 | 0.0004 | 0.0004 | 0.0006 | – | – | – | – | – | – |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 70.2839 | 54.7987 | 25.2391 | 37.0792 | 25.3509 | 64.4353 | 43.4657 | 42.2316 | 49.6423 | 48.0426 |
Translational Efficiency | 1.6882 | 1.1037 | 1.3661 | 1.2309 | 1.5055 | 2.0887 | 1.3536 | 1.2446 | 1.2706 | 1.1006 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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