Standard name
Human Ortholog
Description Conserved mitochondrial protein of unknown function; processed by both mitochondrial processing peptidase and mitochondrial octapeptidyl aminopeptidase; gene contains the nested antisense gene NAG1
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.05 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0.05 0.16 0.16 0.18 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.08 0 0 0.05 0 0 0 0 0 0 0 0.05 0.06 0.07 0 0 0 0 0.05 0
Golgi 0 0 0.2 0.19 0.12 0 0 0 0 0 0 0.05 0.27 0.38 0.52 0.31 0.41 0.28 0 0.08 0 0.06 0.06 0
Mitochondria 0.95 0.89 0.84 0.92 0.82 0.87 0.9 0.85 0.93 0.88 0.94 0.8 0.82 0.68 0.58 0.06 0.06 0 0.85 0.75 0.81 0.78 0.71 0.75
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.08 0.08 0 0 0 0.12 0.1 0.06 0.11 0.09 0.11 0.08 0.05 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.07 0 0 0 0 0.05 0.08 0.09 0.07 0.07 0.08 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Peroxisomes 0 0 0.1 0.05 0.06 0 0 0 0 0 0 0 0 0.06 0.05 0.18 0.16 0.22 0.05 0 0.07 0.07 0.08 0.08
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.08 0.1 0.06 0 0.1 0.07 0.05 0 0 0 0 0.1 0.09 0.11 0.12 0.21 0.17 0.08 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 4 4 2 0 0 2 0 1 2 0 1 4 5 8 5 3 6 3 7 1 5 10 17
Bud 3 1 8 1 6 3 9 10 11 8 13 8 0 2 1 10 1 10 0 1 1 0 2 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 2 0
Bud Site 0 0 0 1 0 2 5 0 3 1 1 1 0 0 0 3 1 0 – – – – – –
Cell Periphery 7 14 14 11 5 6 4 3 1 2 2 7 17 15 31 41 33 29 0 0 2 0 1 3
Cytoplasm 2 7 3 7 6 9 10 7 7 6 6 9 10 20 15 9 9 7 0 0 0 0 0 1
Endoplasmic Reticulum 4 4 4 0 3 0 2 0 2 3 0 2 4 6 9 7 5 5 0 0 0 0 0 2
Endosome 1 4 11 1 27 13 6 20 1 9 1 10 7 26 26 12 12 11 6 8 8 7 18 7
Golgi 3 12 50 44 38 7 10 2 0 6 0 15 104 219 312 76 84 46 12 22 11 15 23 16
Mitochondria 276 244 214 211 262 322 420 366 321 378 295 244 319 399 348 14 12 7 250 205 235 209 261 320
Nucleus 0 1 0 0 1 3 5 4 0 1 0 3 1 1 1 3 2 0 0 1 0 0 0 1
Nuclear Periphery 24 22 11 6 11 46 46 26 39 40 34 23 20 11 12 5 5 7 1 3 1 3 5 4
Nucleolus 21 10 7 5 12 18 39 37 25 29 25 13 5 6 1 5 5 12 0 0 1 1 0 3
Peroxisomes 5 5 25 12 20 7 5 12 2 6 3 10 8 33 28 46 33 36 15 10 20 19 27 35
SpindlePole 0 0 1 1 6 5 3 6 4 6 4 3 2 7 4 11 7 2 1 2 1 1 2 0
Vac/Vac Membrane 24 28 15 10 33 26 24 15 11 17 7 31 36 66 73 53 35 13 1 7 3 2 7 7
Unique Cell Count 292 273 256 229 321 372 467 429 346 428 315 304 387 584 599 249 203 164 296 273 291 269 368 429
Labelled Cell Count 371 356 367 312 430 467 590 508 428 514 391 380 537 816 869 300 248 191 296 273 291 269 368 429


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.9 10.4 9.3 8.8 8.6 7.5 7.5 7.8 6.8 7.5 7.0 9.0 9.1 9.5 9.4 9.5 10.6 9.7 9.9 10.2 10.6
Std Deviation (1e-4) 1.4 2.2 1.8 1.8 1.5 1.7 1.7 1.6 1.7 1.7 1.8 7.4 1.5 1.7 1.5 2.3 2.5 2.2 2.3 2.3 2.2
Intensity Change (Log2) – – – -0.07 -0.11 -0.3 -0.3 -0.25 -0.44 -0.3 -0.39 -0.05 -0.02 0.04 0.02 0.04 0.19 0.06 0.1 0.14 0.2


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 -1.0 0 -1.0 -0.6 0 -1.1 0.6 -0.3 0 0 0 0.5 0 1.5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.3 -2.6 -2.7 -3.8 -3.9 -4.0 -4.2 -3.5 -2.0 -0.6 -2.1 -0.2 4.0 3.8 4.0
Cytoplasm 0 0 0 0 0 0 0 0 1.5 1.2 1.8 1.2 1.8 2.2 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 1.0 0 0
Endosome -2.7 2.0 -0.5 -2.6 0.2 -3.5 -1.6 -3.3 -0.6 -1.9 0.1 0 0.3 0.8 1.1
Golgi -0.1 -2.6 -7.6 -8.1 -9.1 -8.6 -8.4 -8.2 -5.4 2.1 5.1 8.8 2.9 5.1 2.0
Mitochondria 2.9 -0.6 1.0 2.5 0.6 3.5 1.8 3.8 -1.0 -0.4 -4.6 -7.2 -17.6 -16.5 -15.9
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -1.0 -0.5 3.5 2.6 1.0 3.1 2.4 2.9 1.6 0.5 -2.0 -1.9 -1.5 -1.1 0
Nucleolus -0.4 0.7 1.3 3.0 3.0 2.4 2.3 2.7 1.0 0 0 0 -0.5 -0.2 2.2
Peroxisomes -1.9 -1.6 -4.4 -5.6 -3.9 -5.4 -5.1 -4.9 -3.2 -4.3 -2.2 -2.8 2.8 2.1 3.5
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 3.0 0 0
Vacuole -0.7 1.9 0.6 -0.4 -1.5 -1.6 -1.1 -2.2 1.9 1.6 2.5 2.8 5.1 3.9 0.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.1388 5.0175 4.7799 4.7337 4.5373 4.9498 4.5246 5.217 4.9037 4.7733 3.7435 4.8198 6.3427 5.8128 5.7448 5.591 4.952 5.5864
Actin 0.0021 0.0044 0.0013 0.0001 0.0001 0.0017 0.0532 0.0126 0.0111 0.0294 0.004 0.0048 0.0096 0.008 0.0024 0.0002 0.0346 0.001
Bud 0.0027 0.0025 0.0027 0.001 0 0.0001 0.0011 0.0013 0.0005 0.0003 0 0.0001 0.0002 0.0007 0.0002 0 0.0001 0.0001
Bud Neck 0.0002 0.0003 0.0001 0 0 0.0001 0.0044 0.0005 0.0006 0.0008 0.0001 0.0001 0.0002 0.0003 0.0003 0 0.0009 0.0001
Bud Periphery 0.0025 0.0028 0.0016 0.0059 0.0001 0.0001 0.0019 0.0022 0.0009 0.0008 0.0001 0.0002 0.0005 0.0009 0.0004 0.0002 0.0003 0.0002
Bud Site 0.0023 0.0107 0.0042 0.0002 0 0.0001 0.0129 0.0206 0.001 0.0013 0.0001 0.0003 0.0005 0.0085 0.0004 0 0.0002 0.0001
Cell Periphery 0.0005 0.0007 0.0002 0.0004 0 0.0001 0.0012 0.0021 0.0005 0.0003 0 0.0001 0.0004 0.0004 0.0003 0.0001 0.0002 0.0001
Cytoplasm 0.0013 0.0013 0.0001 0 0 0.0001 0.0116 0.0009 0.0053 0.0002 0.0046 0.0006 0.001 0.0003 0.0001 0.0001 0.0002 0
Cytoplasmic Foci 0.0072 0.0097 0.0012 0.0004 0.0004 0.0052 0.0171 0.0134 0.0067 0.0039 0.0052 0.0067 0.0044 0.0066 0.0015 0.0008 0.0008 0.0014
Eisosomes 0.0002 0.0002 0.0001 0.0001 0.0002 0.0001 0.0008 0.0004 0.0004 0.0002 0.0001 0.0001 0.0006 0.0002 0.0002 0.0002 0.0008 0.0001
Endoplasmic Reticulum 0.0004 0.0018 0.0001 0 0.0001 0.0002 0.0072 0.0004 0.0053 0.0008 0.0013 0.0003 0.0006 0.0002 0.0001 0.0001 0.0019 0.0001
Endosome 0.0128 0.0229 0.0081 0.0012 0.0106 0.0121 0.046 0.0227 0.0201 0.0163 0.0082 0.0165 0.0104 0.0184 0.0078 0.0157 0.0065 0.0072
Golgi 0.0106 0.0253 0.0086 0.001 0.0095 0.0396 0.0225 0.0324 0.0185 0.0101 0.0011 0.0185 0.0143 0.033 0.0199 0.0306 0.0074 0.0228
Lipid Particles 0.0171 0.012 0.0024 0.0004 0.0006 0.0093 0.0387 0.0074 0.0092 0.0145 0.0015 0.0088 0.0201 0.0089 0.0019 0.001 0.0038 0.0024
Mitochondria 0.9086 0.8431 0.9545 0.9833 0.9715 0.9241 0.6761 0.8436 0.892 0.8924 0.9072 0.9239 0.8973 0.8936 0.9563 0.9463 0.932 0.957
None 0.0003 0.0018 0 0.0001 0 0 0.0101 0.0002 0.0009 0.0003 0.0025 0.0004 0.0054 0.0001 0 0 0.0009 0
Nuclear Periphery 0.0009 0.0024 0 0 0 0.0001 0.0255 0.0005 0.0039 0.0072 0.0219 0.0014 0.0028 0.0001 0 0.0001 0.0042 0.0001
Nucleolus 0.0011 0.001 0.0001 0.0002 0 0.0001 0.0068 0.001 0.0003 0.0007 0.0002 0.0001 0.0011 0.0015 0.0001 0 0.0002 0.0014
Nucleus 0.0007 0.0014 0.0001 0.0002 0 0 0.022 0.0006 0.0016 0.001 0.0041 0.0002 0.0013 0.0002 0.0001 0.0001 0.0005 0.0004
Peroxisomes 0.0184 0.0121 0.01 0.0012 0.0034 0.0038 0.0119 0.0168 0.0137 0.01 0.0009 0.011 0.011 0.0105 0.0043 0.0005 0.0014 0.0023
Punctate Nuclear 0.0003 0.0005 0 0 0 0 0.0113 0.0001 0.0012 0.0006 0.0357 0.0026 0.0028 0.0002 0 0 0.0013 0
Vacuole 0.0044 0.0215 0.0019 0.0021 0.0009 0.0007 0.0092 0.0119 0.0038 0.0038 0.0002 0.0008 0.0071 0.0029 0.0013 0.0011 0.0004 0.0009
Vacuole Periphery 0.0055 0.0216 0.0026 0.002 0.0024 0.0021 0.0085 0.0082 0.0026 0.0052 0.0009 0.0024 0.0084 0.0044 0.0025 0.0028 0.0014 0.0023

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.0846 25.8816 19.9166 18.8821 24.3301 15.004 19.3843 22.8955 23.561 16.9887
Translational Efficiency 0.8809 0.4797 0.7588 1.0521 0.5416 0.7279 0.7528 0.6683 0.6891 0.7525

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Conserved mitochondrial protein of unknown function; processed by both mitochondrial processing peptidase and mitochondrial octapeptidyl aminopeptidase; gene contains the nested antisense gene NAG1
Localization
Cell Percentages mitochondrion (98%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Imo32

Imo32


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Imo32-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available