Standard name
Human Ortholog
Description Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region; required for H3K36 trimethylation but not dimethylation by Set2p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0.07 0 0.05 0 0.05 0.08 0.07 0 0 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.05 0.05 0 0.09 0.13 0 0.16 0.15 0.08 0.07 0 0 0 0 0 0.07 0 0 0 0 0 0
Nucleus 0.81 0.83 0.76 0.84 0.83 0.75 0.69 0.74 0.55 0.64 0.59 0.67 0.82 0.79 0.74 0.75 0.78 0.73 0.62 0.78 0.62 0.67 0.64 0.47
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.59 0.83 0.53 0.36 0.44 0.33 0.52 0.5 0.49 0.46 0.57 0.6 0.64 0.4 0.42 0.43 0.34 0.38 0.32 0.16 0.32 0.3 0.26 0.42
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 0 0 0 0 0 0 0 0 0 0 0 1 8 0 0 0 4 0 0 0 2 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 1 0 0 1 3 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 1 1 4 7 7 1 2 1 5 1 6 13 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 1 0 3 2 2 3 5 7 5 5 3 8 32 30 8 6 13 0 0 0 0 0 1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 2 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 3 3 2 1 0 0 0 0 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 1
Mitochondria 4 2 2 5 5 10 28 8 17 26 9 12 2 1 8 6 7 13 1 0 0 0 0 5
Nucleus 223 67 29 91 144 79 154 183 57 113 63 121 125 305 300 162 135 145 177 77 42 85 198 164
Nuclear Periphery 0 0 0 0 1 2 5 1 2 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 161 67 20 39 76 35 116 124 51 81 60 109 97 153 171 92 59 76 91 15 22 38 80 147
Peroxisomes 1 0 1 0 0 0 0 0 0 0 0 0 0 2 6 1 1 2 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 1 17 17 1 1 0 1 0 0 0 3 4
Vac/Vac Membrane 0 0 0 0 0 1 1 0 0 1 0 0 0 3 6 4 5 3 3 3 1 2 23 16
Unique Cell Count 275 81 38 108 174 106 223 248 104 176 106 181 152 385 405 216 173 200 285 99 69 129 311 350
Labelled Cell Count 392 138 52 139 229 133 314 328 136 231 142 250 235 521 564 281 218 256 285 99 69 129 311 350


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 23.7 16.9 13.0 12.3 11.5 11.7 10.2 10.2 10.4 10.0 9.8 9.1 19.1 18.7 15.4 21.2 23.0 25.6 19.6 19.4 21.5
Std Deviation (1e-4) 7.4 5.0 3.6 3.4 2.8 3.1 2.7 2.9 4.0 3.1 4.7 2.9 6.8 7.5 6.3 7.6 8.4 9.3 5.6 6.6 5.9
Intensity Change (Log2) -0.08 -0.18 -0.15 -0.35 -0.36 -0.33 -0.38 -0.4 -0.52 0.56 0.52 0.24 0.7 0.82 0.97 0.59 0.58 0.72


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 1.7 0 0 0 0 0 0 0 0 0
Nucleus 1.1 0.9 -0.2 -0.9 -0.3 -2.3 -1.4 -1.9 -1.1 0.8 0.4 -0.3 -0.2 0.2 -0.5
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -1.8 -1.0 -2.1 -0.1 -0.3 -0.4 -0.7 0.4 0.9 1.3 -1.5 -1.2 -1.1 -2.1 -1.7
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 11.1938 13.9172 14.3029 13.0493 12.191 12.6741 20.8784 21.8336 19.0115 17.5458 19.4047 19.2989 13.4348 15.5206 13.9032 15.8125 10.5077 13.9661
Actin 0.0462 0 0.0299 0 0.1129 0.0219 0.0106 0 0.0078 0 0.0079 0.0002 0.0948 0.0002 0.0107 0 0.0312 0.0028
Bud 0.0007 0 0.0001 0 0.0023 0.0003 0.0002 0 0.0005 0.0001 0.0003 0.0001 0.0002 0.0013 0.0005 0 0.0005 0
Bud Neck 0.001 0 0.0002 0 0.0045 0.0009 0.0001 0 0.0001 0 0.0001 0.0002 0.002 0.0001 0.0001 0 0.0113 0.0002
Bud Periphery 0.0009 0 0.0001 0 0.003 0.0003 0.0003 0 0.0007 0.0004 0.0006 0.0001 0.0002 0.0027 0.0008 0 0.0009 0.0001
Bud Site 0.0044 0.0001 0.0002 0 0.01 0.0017 0.0002 0.0001 0.0012 0.0001 0.0003 0.0001 0.0031 0.0032 0.0032 0 0.003 0.0001
Cell Periphery 0.0004 0 0 0 0.0005 0.0002 0.0001 0.0001 0.0001 0.0006 0.0008 0.0001 0.0002 0.0005 0.0004 0 0.0011 0
Cytoplasm 0.0221 0 0.0003 0 0.0015 0.0018 0.0051 0 0.0004 0.0001 0 0.0001 0.0042 0.0003 0.0019 0 0.0023 0.0003
Cytoplasmic Foci 0.0194 0 0.0044 0 0.0268 0.0108 0.0027 0.0005 0.0004 0.0002 0.0011 0 0.01 0 0.0024 0 0.0159 0.0011
Eisosomes 0.0004 0 0.0001 0 0.0005 0.0003 0.0001 0.0001 0.0001 0 0.0002 0 0.0005 0 0.0004 0 0.0004 0
Endoplasmic Reticulum 0.0125 0 0.0022 0 0.0036 0.0009 0.001 0 0.0005 0 0.0001 0 0.0013 0.0001 0.0013 0 0.0051 0.0003
Endosome 0.0382 0 0.0056 0 0.0299 0.0063 0.0045 0 0.0026 0.0001 0.0031 0.0002 0.0032 0.0005 0.0036 0 0.0188 0.0036
Golgi 0.0083 0 0.0065 0 0.0194 0.0145 0.0014 0 0.0006 0 0.0092 0 0.0026 0 0.0028 0 0.0083 0.0006
Lipid Particles 0.0103 0 0.0013 0 0.0133 0.0061 0.001 0.0002 0.0003 0.0005 0.0194 0 0.0058 0.0001 0.0065 0 0.0242 0.001
Mitochondria 0.021 0.0016 0.0016 0.0011 0.0614 0.0151 0.0044 0.0035 0.0038 0.0044 0.0714 0.0009 0.0006 0.0021 0.0068 0.0003 0.0161 0.0006
None 0.0066 0 0.0003 0 0.0015 0.0054 0.0047 0.0002 0.0003 0.0002 0.0002 0.0001 0.0032 0.0002 0.0053 0 0.0035 0.0003
Nuclear Periphery 0.0353 0.0002 0.0022 0.0001 0.0038 0.0015 0.0193 0.0003 0.0086 0.0016 0.0017 0.0031 0.0044 0.0019 0.0005 0.0003 0.0041 0.0036
Nucleolus 0.215 0.1309 0.1049 0.0719 0.408 0.303 0.1378 0.0891 0.0781 0.1263 0.5508 0.2935 0.1252 0.0391 0.0691 0.1056 0.4918 0.3115
Nucleus 0.5245 0.8664 0.8355 0.9264 0.2491 0.5898 0.7912 0.9052 0.8867 0.788 0.3232 0.6987 0.6978 0.9389 0.8801 0.8934 0.3406 0.668
Peroxisomes 0.0045 0 0.004 0 0.0416 0.002 0.0004 0.0001 0.0003 0.0001 0.0045 0 0.008 0.0001 0.0016 0 0.0079 0.0004
Punctate Nuclear 0.0234 0.0001 0.0003 0.0001 0.0018 0.0149 0.0137 0 0.0044 0.0662 0.0007 0.0023 0.0318 0.0001 0.0004 0.0001 0.0109 0.0052
Vacuole 0.0023 0.0001 0.0001 0.0001 0.002 0.0007 0.0004 0.0001 0.0011 0.0029 0.0014 0.0002 0.0008 0.0057 0.0009 0 0.0013 0.0001
Vacuole Periphery 0.0024 0.0004 0.0002 0.0001 0.0025 0.0016 0.0011 0.0003 0.0014 0.0079 0.0029 0.0002 0.0002 0.003 0.0007 0.0001 0.0008 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 102.8426 102.3512 92.0798 100.4852 94.7501 89.1146 84.5413 89.3948 89.9389 95.0235
Translational Efficiency 1.2865 1.2525 1.1789 1.2178 1.0611 1.1494 1.0959 0.9043 0.891 0.9978

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region; required for H3K36 trimethylation but not dimethylation by Set2p
Localization
Cell Percentages nucleus (87%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Spt6

Spt6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Spt6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available