Standard name
Human Ortholog
Description Nucleolar protein that binds nuclear localization sequences; required for pre-rRNA processing and ribosome biogenesis; binds to single stranded telomeric DNA and mRNA; methylated by Hmt1p; interaction with Top1p and nucleolar localization are negatively regulated by polyphosphorylation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.11 0.13 0.11 0.1 0.15 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.08 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0.1 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Mitochondria 0.5 0.23 0.47 0.43 0.3 0.59 0.51 0.36 0.4 0.46 0.46 0.44 0.21 0.18 0.24 0.12 0.08 0.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.23 0.22 0.17 0.08 0.1 0 0.07 0.06 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.98 0.94 0.93 0.94 0.89 0.93 0.93 0.9 0.9 0.87 0.86 0.85 0.86 0.83 0.82 0.61 0.7 0.65 0.74 0.63 0.64 0.73 0.75 0.66
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.1 0.14 0.06 0.06 0.11
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 2 1 2 0 2 1 0 1 0 0 0 0 0 0 0 0 1
Bud 0 0 0 0 0 0 0 4 2 2 2 1 0 0 1 0 0 0 0 0 3 4 14 12
Bud Neck 0 0 0 0 0 0 0 1 0 1 0 0 0 2 0 0 0 0 0 0 3 2 4 8
Bud Site 0 0 0 0 1 1 2 0 4 6 3 10 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 2 6 10 8 12 13 10 4 4 9 42 74 93 27 22 40 0 0 3 0 0 2
Endoplasmic Reticulum 0 0 0 0 1 0 0 1 0 1 0 0 0 0 0 15 17 19 0 0 0 0 0 3
Endosome 0 0 2 1 0 2 0 1 2 9 1 6 2 2 5 1 0 0 0 10 8 7 8 21
Golgi 0 1 8 2 35 0 1 8 1 2 2 1 22 22 7 13 4 10 1 3 6 10 3 15
Mitochondria 68 54 135 107 103 249 283 210 162 203 151 155 128 119 176 29 17 26 1 0 0 5 2 7
Nucleus 1 5 4 0 2 7 10 7 7 7 10 8 30 22 14 54 48 47 11 24 13 33 33 28
Nuclear Periphery 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 3 1 5 8 17
Nucleolus 132 216 267 232 301 393 510 532 368 386 281 302 519 539 595 144 154 177 104 156 194 364 438 500
Peroxisomes 0 0 0 3 1 0 0 2 0 0 0 0 2 3 3 0 0 1 0 0 6 3 1 7
SpindlePole 0 0 1 0 0 0 0 2 2 2 2 1 4 3 1 0 0 0 9 24 42 31 36 79
Vac/Vac Membrane 3 9 6 3 13 6 12 18 14 16 13 10 2 5 13 12 8 7 3 12 10 16 17 25
Unique Cell Count 135 230 287 247 340 421 551 588 409 446 328 356 600 650 724 236 220 273 141 247 306 502 583 754
Labelled Cell Count 204 285 425 354 467 666 832 801 573 641 469 505 752 794 910 295 270 327 141 247 306 502 583 754


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 72.1 70.0 71.1 72.5 62.0 41.7 39.2 36.8 33.5 30.1 31.0 30.3 87.3 81.3 71.3 148.0 132.0 139.5 103.2 98.8 93.9
Std Deviation (1e-4) 21.8 18.4 19.4 20.6 24.3 11.9 12.2 11.4 10.5 9.8 9.4 9.5 28.6 31.8 29.2 46.5 42.5 48.1 31.6 32.3 29.2
Intensity Change (Log2) – – – 0.03 -0.2 -0.77 -0.86 -0.95 -1.09 -1.24 -1.2 -1.23 0.3 0.19 0.0 1.06 0.89 0.97 0.54 0.47 0.4


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 2.0 0 0 0 0 0 0 0 4.0 5.5 6.0 5.3 4.9 6.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 4.3 4.8 4.5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 3.7 0 0 -1.5 0 0 0 0 0.7 0.5 0 1.6 -0.7 0.6
Mitochondria -0.9 -4.3 3.2 1.2 -3.2 -2.0 -0.4 -0.2 -0.9 -7.8 -9.1 -7.1 -8.5 -9.6 -9.8
Nucleus 0 0 0 0 0 0 0 1.4 0.8 2.6 1.7 0.6 7.8 7.5 6.5
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.4 -1.9 0.2 -0.2 -1.3 -1.4 -2.7 -2.9 -3.2 -2.9 -4.1 -4.4 -8.9 -6.8 -8.2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 1.3 0 0.1 0.8 1.0 1.2 1.3 0.6 0 0 -0.3 1.9 1.1 0.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 83.7006 113.5037 98.4218 107.5842 96.4841 99.406 72.3758 111.6533 90.9537 89.8632 77.2915 100.8164 89.2073 128.6561 106.6419 101.6233 111.6202 107.6556
Actin 0.005 0 0.0022 0 0.0175 0 0.0063 0 0.0005 0 0.001 0.0012 0.0119 0.0024 0.0123 0.0002 0.0004 0.0265
Bud 0.0001 0 0.0001 0.0001 0.0004 0 0.0005 0.0001 0.0001 0 0.0005 0.0001 0.0003 0.0001 0.0002 0.0002 0.0001 0.0001
Bud Neck 0.0002 0.0001 0.0001 0.0001 0.0055 0.0002 0.0002 0.0001 0.0003 0.0002 0.0008 0.0001 0.0003 0.0001 0.0002 0.0002 0.0003 0.0001
Bud Periphery 0.0001 0 0 0 0.0005 0 0.0003 0 0.0001 0 0.0003 0.0002 0.0004 0.0001 0.0002 0.0002 0.0001 0.0001
Bud Site 0.0006 0.0001 0.0002 0.0001 0.0004 0.0001 0.0011 0.0002 0.0013 0.0003 0.0022 0.0001 0.0015 0.0011 0.0006 0.0005 0.0003 0.0002
Cell Periphery 0.0005 0.0002 0.0003 0.0016 0.0018 0.0002 0.0004 0.0004 0.0004 0.0006 0.0009 0.0001 0.001 0.0002 0.0003 0.0009 0.0008 0.0001
Cytoplasm 0.0013 0.0002 0.0039 0.0007 0.007 0.0001 0.0033 0.0002 0.0006 0.0003 0.0015 0.0002 0.0063 0.0015 0.0021 0.0009 0.0024 0.0002
Cytoplasmic Foci 0.0156 0.0001 0.0032 0.0015 0.0118 0.0036 0.0109 0.0055 0.0084 0.0196 0.0895 0.0008 0.0224 0.0057 0.0319 0.0185 0.0271 0.0038
Eisosomes 0.0002 0 0.0001 0 0.0004 0 0.0001 0 0 0 0.0013 0 0.0015 0 0.0002 0.0002 0.0002 0.0001
Endoplasmic Reticulum 0.0008 0 0.0001 0 0.0003 0 0.0008 0 0.0078 0.0001 0 0.0017 0.0037 0.0001 0.0005 0.0003 0.0002 0.0004
Endosome 0.0031 0 0.0006 0.0002 0.0023 0.0001 0.0067 0 0.0056 0.0105 0.0013 0.016 0.01 0.0005 0.0028 0.0011 0.0006 0.0027
Golgi 0.001 0 0.0005 0 0.004 0.0001 0.0019 0 0.0027 0.002 0.0013 0.0058 0.007 0.0003 0.0026 0.0004 0.0006 0.0022
Lipid Particles 0.0084 0.0001 0.0032 0.0003 0.0104 0.0012 0.009 0.0005 0.0167 0.0138 0.0218 0.0011 0.0094 0.0007 0.0121 0.0084 0.0104 0.0044
Mitochondria 0.0058 0.0003 0.0024 0.0003 0.0052 0.0011 0.0112 0.0016 0.0023 0.0013 0.0338 0.0019 0.0129 0.0036 0.0044 0.0075 0.007 0.0027
None 0.003 0 0.0024 0 0.001 0.0001 0.0053 0.0002 0.0001 0.0001 0.0063 0.0001 0.0096 0.0003 0.0049 0.0043 0.0064 0.0008
Nuclear Periphery 0.0019 0.0004 0.0011 0.0002 0.0062 0.0008 0.0098 0.0001 0.0013 0.0003 0.0006 0.0089 0.0077 0.0011 0.0003 0.001 0.0067 0.0022
Nucleolus 0.818 0.8203 0.8524 0.8 0.779 0.8816 0.7751 0.8496 0.8741 0.8261 0.7549 0.868 0.7852 0.8702 0.8332 0.8883 0.7965 0.8316
Nucleus 0.1112 0.1771 0.1124 0.1049 0.1229 0.0955 0.1308 0.1386 0.0688 0.1076 0.0633 0.0794 0.0897 0.1096 0.0827 0.0586 0.1065 0.1158
Peroxisomes 0.0017 0 0.008 0 0.004 0.0001 0.004 0.0001 0.002 0.0008 0.0049 0.0001 0.0051 0.0003 0.0056 0.0012 0.0005 0.0023
Punctate Nuclear 0.016 0.0007 0.0037 0.0131 0.0121 0.0117 0.0195 0.0012 0.0048 0.0109 0.0084 0.0119 0.0115 0.0009 0.0016 0.0033 0.0296 0.0026
Vacuole 0.0025 0.0003 0.0017 0.0573 0.0033 0.0008 0.0012 0.0009 0.0011 0.0025 0.0022 0.0008 0.0012 0.0006 0.0009 0.0019 0.0012 0.0004
Vacuole Periphery 0.0028 0.0004 0.0012 0.0194 0.0039 0.0025 0.0017 0.0005 0.0009 0.0031 0.003 0.0016 0.0015 0.0004 0.0008 0.0018 0.0021 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 401.0366 503.8837 405.0621 198.5808 357.3658 311.7939 236.9145 134.8956 147.461 205.3423
Translational Efficiency 2.6263 2.278 2.0896 3.0783 2.0396 1.8077 1.9912 2.7492 1.5932 2.0158

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Nucleolar protein that binds nuclear localization sequences; required for pre-rRNA processing and ribosome biogenesis; binds to single stranded telomeric DNA and mRNA; methylated by Hmt1p; interaction with Top1p and nucleolar localization are negatively regulated by polyphosphorylation
Localization
Cell Percentages nucleolus (46%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Nsr1

Nsr1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nsr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available