Standard name
Human Ortholog
Description Voltage-gated high-affinity calcium channel; involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and partially co-localizes with Mid1p; however, evidence suggests CCH1 is not required for Mid1p function
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.07 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0.07 0.08 0.05 0.1 0 0.06 0.14 0.18 0.17
Bud 0 – 0.06 0 0 0.05 – 0.05 0.06 0 0.07 0.06 0 0 0 0 0 0 0 0.05 0 0.1 0.11 0.09
Bud Neck 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.06 0.1 0.07 0.12 0.08 0.06
Bud Site 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.67 – 0.84 0.78 0.76 0.72 – 0.62 0.55 0.64 0.56 0.45 0.88 0.83 0.86 0.81 0.74 0.84 0.29 0.39 0.38 0.21 0.24 0.3
Endoplasmic Reticulum 0.11 – 0 0 0 0 – 0 0 0 0 0.05 0.05 0 0 0.06 0.06 0.1 0.07 0.05 0 0 0.07 0.05
Endosome 0 – 0 0 0 0 – 0 0 0 0 0 0 0.1 0.08 0.05 0.08 0 0.06 0 0 0 0.05 0
Golgi 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.14 – 0.12 0.25 0.09 0.27 – 0.37 0.59 0.43 0.54 0.63 0 0 0 0 0 0 0.25 0.15 0.27 0.22 0.15 0.14
Nucleus 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
Nuclear Periphery 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Vac/Vac Membrane 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.06 0 0.09 0.14 0.08 0.09 0.08 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 18 – 1 7 14 2 – 2 0 6 7 12 9 12 5 10 12 8 30 0 8 13 59 65
Bud 8 – 6 9 16 22 – 41 21 21 28 29 4 22 8 2 5 3 13 1 6 10 34 35
Bud Neck 2 – 3 0 3 1 – 7 3 8 0 1 0 0 1 0 0 0 18 4 9 12 24 22
Bud Site 1 – 1 6 5 4 – 7 3 5 9 4 1 0 0 1 2 0 – – – – – –
Cell Periphery 2 – 1 5 0 3 – 1 0 5 3 6 1 4 1 4 6 1 2 0 1 1 2 5
Cytoplasm 165 – 92 186 300 333 – 470 183 304 232 224 208 417 422 121 106 146 90 16 52 21 79 118
Endoplasmic Reticulum 27 – 1 1 14 7 – 5 0 1 6 26 12 5 10 9 8 17 20 2 2 1 22 19
Endosome 7 – 0 1 17 12 – 18 0 2 2 0 7 52 39 7 11 4 17 1 4 4 15 14
Golgi 0 – 0 0 0 0 – 0 0 0 0 0 1 0 1 3 2 2 4 0 0 0 1 4
Mitochondria 34 – 13 60 36 127 – 283 195 206 223 311 5 9 7 3 4 1 76 6 37 21 47 55
Nucleus 2 – 0 0 0 0 – 4 1 3 0 2 0 3 2 1 0 1 0 1 0 0 0 0
Nuclear Periphery 0 – 0 1 0 0 – 3 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1 – 0 0 0 0 – 3 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 – 0 5 9 5 – 3 0 2 0 1 1 12 4 3 2 0 1 0 0 0 2 0
SpindlePole 1 – 0 4 11 11 – 29 10 13 8 4 2 3 6 4 3 9 1 0 1 0 3 4
Vac/Vac Membrane 4 – 0 0 8 9 – 8 3 7 4 6 0 5 8 6 8 2 29 5 11 8 27 37
Unique Cell Count 247 109 239 397 465 755 331 475 416 495 237 501 489 150 143 174 311 43 137 99 325 390
Labelled Cell Count 272 118 285 433 536 884 419 585 522 627 251 544 514 174 169 194 311 43 137 99 325 390


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 – 3.6 3.8 4.2 3.4 – 3.6 3.1 3.4 3.2 3.1 5.2 5.3 5.2 7.1 7.3 6.7 5.1 5.6 5.5
Std Deviation (1e-4) 0.4 – 0.7 0.9 0.8 0.7 – 1.0 0.9 0.7 0.6 0.6 0.9 1.2 1.2 1.3 2.0 1.5 1.2 1.7 1.3
Intensity Change (Log2) – – – 0.11 0.25 -0.06 – 0.01 -0.21 -0.06 -0.16 -0.21 0.55 0.59 0.54 0.99 1.04 0.92 0.53 0.66 0.63


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 2.7 0
Bud 0 0 0 – 0 0 0 0 0 0 -0.5 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.4 -2.0 -2.7 – -4.5 -5.4 -4.1 -5.5 -7.4 0.9 -0.3 0.5 -0.8 -2.0 -0.1
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 3.0
Endosome 0 0 0 – 0 0 0 0 0 0 3.5 3.0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 0 0 0 0 0 -3.8 0 0 -3.3 -2.9 -4.3
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.7113 0.7489 0.6803 0.4056 0.6691 0.6323 -0.1639 -0.0139 -0.241 -0.2507 -0.1042 -0.0149 1.7454 1.8269 1.8563 2.3892 2.0187 1.748
Actin 0.0411 0.0112 0.0263 0.0016 0.0474 0.0331 0.0161 0.0024 0.0037 0.0013 0.0066 0.0127 0.0573 0.0134 0.0153 0.0051 0.0269 0.0072
Bud 0.0047 0.0034 0.0047 0.0042 0.0032 0.0032 0.001 0.0014 0.0012 0.0005 0.0014 0.0006 0.0013 0.0009 0.0023 0.0004 0.0042 0.0011
Bud Neck 0.0059 0.0067 0.0031 0.0008 0.0023 0.0933 0.0026 0.0057 0.0119 0.0007 0.0015 0.0738 0.0176 0.0012 0.0022 0.0013 0.009 0.1572
Bud Periphery 0.0143 0.006 0.0078 0.0047 0.005 0.0049 0.001 0.0014 0.0013 0.0003 0.0018 0.0011 0.0026 0.0011 0.0033 0.0004 0.0053 0.0011
Bud Site 0.0261 0.0877 0.0191 0.0014 0.0082 0.0029 0.0193 0.0455 0.0038 0.0004 0.0009 0.0008 0.0182 0.0389 0.0275 0.001 0.0272 0.0017
Cell Periphery 0.0015 0.0013 0.0008 0.0004 0.0005 0.0005 0.0012 0.0013 0.0006 0.0002 0.0003 0.0005 0.0024 0.002 0.0008 0.0002 0.0007 0.0006
Cytoplasm 0.2761 0.3458 0.3422 0.2469 0.2143 0.3925 0.3118 0.5035 0.4112 0.4589 0.4972 0.4899 0.2928 0.5282 0.4486 0.5269 0.4435 0.517
Cytoplasmic Foci 0.1134 0.0712 0.1285 0.1024 0.0781 0.107 0.1343 0.066 0.1345 0.1245 0.0954 0.0967 0.0883 0.0561 0.0882 0.0675 0.1214 0.0762
Eisosomes 0.0006 0.0004 0.0004 0.0003 0.0006 0.0002 0.0004 0.0002 0.0002 0 0 0.0002 0.0013 0.0003 0.0003 0.0001 0.0004 0.0001
Endoplasmic Reticulum 0.0085 0.0063 0.007 0.0143 0.0087 0.0054 0.0196 0.0099 0.0081 0.0137 0.0113 0.0061 0.0254 0.013 0.0093 0.0071 0.0079 0.0085
Endosome 0.0821 0.0509 0.0874 0.1582 0.0567 0.0476 0.1515 0.0582 0.149 0.217 0.1715 0.0726 0.0683 0.0339 0.0786 0.069 0.086 0.0244
Golgi 0.0186 0.0077 0.0133 0.0133 0.019 0.0073 0.0229 0.0083 0.0188 0.0168 0.0306 0.0134 0.0122 0.007 0.0168 0.0032 0.0167 0.0082
Lipid Particles 0.0197 0.0063 0.0089 0.0253 0.0297 0.0046 0.0141 0.0066 0.004 0.0036 0.0052 0.006 0.0263 0.0026 0.0081 0.0065 0.0292 0.0013
Mitochondria 0.018 0.0045 0.0087 0.0103 0.0174 0.01 0.0174 0.0053 0.0044 0.0035 0.0426 0.0127 0.0083 0.0014 0.0127 0.0019 0.0086 0.0027
None 0.3182 0.3718 0.2944 0.3609 0.4385 0.2618 0.247 0.2622 0.2261 0.1308 0.118 0.1964 0.3072 0.2898 0.2498 0.1738 0.1157 0.1838
Nuclear Periphery 0.0079 0.0015 0.0052 0.0062 0.0152 0.0013 0.008 0.0014 0.0015 0.0018 0.0015 0.0009 0.015 0.0021 0.0046 0.0057 0.0104 0.0005
Nucleolus 0.0021 0.0015 0.0015 0.0009 0.0014 0.0005 0.0008 0.0004 0.0003 0.0002 0.0002 0.0002 0.0018 0.0003 0.0017 0.0086 0.0049 0.0001
Nucleus 0.0052 0.0026 0.008 0.0042 0.0052 0.0023 0.0032 0.0018 0.0015 0.0012 0.0013 0.0011 0.0179 0.0012 0.0045 0.0261 0.0136 0.0007
Peroxisomes 0.0186 0.003 0.0118 0.0041 0.0294 0.0123 0.0132 0.0087 0.0046 0.0022 0.0028 0.0068 0.0121 0.0012 0.011 0.0717 0.0228 0.0049
Punctate Nuclear 0.008 0.0037 0.01 0.0037 0.0129 0.0038 0.0045 0.0012 0.0015 0.0013 0.001 0.0028 0.012 0.0011 0.0054 0.0125 0.0275 0.0007
Vacuole 0.0077 0.0056 0.0089 0.0296 0.0046 0.0047 0.0083 0.0078 0.0098 0.0174 0.0069 0.0036 0.0096 0.0038 0.0069 0.0093 0.0136 0.0016
Vacuole Periphery 0.0017 0.0009 0.002 0.0065 0.0018 0.0009 0.0021 0.0009 0.0021 0.0037 0.0022 0.0011 0.0021 0.0006 0.0022 0.0017 0.0046 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.1783 13.8608 9.3286 12.3428 9.1166 8.289 14.2184 13.9685 11.1668 14.7615
Translational Efficiency 0.3697 0.2225 0.417 0.2787 0.3551 0.4565 0.2725 0.225 0.3114 0.2291

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Voltage-gated high-affinity calcium channel; involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and partially co-localizes with Mid1p; however, evidence suggests CCH1 is not required for Mid1p function
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Cch1

Cch1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cch1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available