Standard name
Human Ortholog
Description Putative RNA binding protein; localizes to stress granules induced by glucose deprivation; interacts with Rbg1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.09 0.07 0.1 0 0 0 0 0 0
Cytoplasm 0.99 0.97 0.99 0.93 0.95 0.92 0.85 0.77 0.62 – 0.52 0.44 0.98 0.97 0.97 0.89 0.89 0.84 0.96 0.95 0.89 0.76 0.81 0.84
Endoplasmic Reticulum 0 0 0 0 0 0 0.05 0.05 0.05 – 0 0 0 0 0 0 0 0.05 0 0 0 0.15 0.1 0.08
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.07 0 0.15 0.16 0.18 0.43 – 0.61 0.55 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.08 0 – 0.05 0.12 0 0 0 0.1 0.09 0.13 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 3 3 1 – 1 2 0 0 0 0 0 1 0 1 0 0 1 0
Bud 1 1 1 1 2 0 2 5 1 – 4 1 0 1 0 0 0 0 0 2 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 8 3 0 6 2 4 1 8 – 6 2 0 7 7 29 22 27 0 0 0 0 0 0
Cytoplasm 281 478 151 52 146 119 186 163 110 – 103 51 123 216 211 280 284 233 277 484 158 66 136 150
Endoplasmic Reticulum 5 18 2 0 3 2 11 10 9 – 8 4 2 2 4 7 11 14 2 6 7 13 16 13
Endosome 0 0 0 0 0 0 0 1 0 – 1 0 0 0 0 3 0 3 1 2 0 1 0 0
Golgi 0 1 0 0 0 0 0 0 0 – 0 0 0 1 0 3 4 3 2 2 0 0 0 1
Mitochondria 0 3 0 4 3 19 36 38 77 – 121 64 0 1 7 5 3 7 0 1 3 2 4 1
Nucleus 0 0 0 2 1 0 0 1 1 – 7 1 0 0 1 1 0 2 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 1 0 0 2 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 0 0 0 1 0 3 18 6 – 9 14 0 0 0 32 30 37 0 6 2 1 5 8
Unique Cell Count 284 495 153 56 154 130 220 212 178 198 117 125 223 217 316 318 278 290 510 178 87 169 179
Labelled Cell Count 292 509 157 60 162 143 245 240 215 260 140 125 228 230 360 355 327 290 510 178 87 169 179


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.3 9.4 7.6 7.1 8.0 6.3 6.7 7.1 6.5 – 5.9 6.4 7.6 8.6 8.8 12.2 12.3 11.4 7.0 7.5 8.5
Std Deviation (1e-4) 1.2 1.9 1.1 1.7 0.9 1.0 1.0 1.0 1.2 – 1.2 1.2 0.9 1.3 1.4 3.2 2.4 3.0 1.2 1.2 1.5
Intensity Change (Log2) – – – -0.09 0.08 -0.27 -0.19 -0.09 -0.23 – -0.37 -0.24 0.01 0.17 0.21 0.68 0.69 0.59 -0.13 -0.01 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 1.3 – 0 0 0 0 0 2.9 2.2 3.0
Cytoplasm -2.2 -1.9 -2.9 -4.6 -5.9 -8.2 – -9.7 -10.3 -0.2 -1.1 -0.9 -3.8 -3.6 -4.7
Endoplasmic Reticulum 0 0 0 1.9 1.8 1.9 – 0 0 0 0 0 0 0 2.0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.9 5.3 5.5 9.3 – 11.9 10.5 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 3.7 0 – 0 4.4 0 0 0 4.1 3.9 4.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.1423 3.9005 3.5451 3.1091 3.1633 3.3299 3.9683 4.5907 4.2419 3.8185 3.2944 4.3607 3.3653 4.0601 3.687 4.0162 3.6771 4.1398
Actin 0.0184 0.0008 0.0038 0.0002 0.0009 0.0008 0.0461 0.0007 0.0401 0.0223 0.0638 0.0007 0.0396 0.001 0.006 0.0135 0.0076 0.0079
Bud 0.0005 0.0014 0.0006 0.0003 0.0006 0.0003 0.0011 0.0003 0.001 0.0021 0.0014 0.0002 0.0034 0.0006 0.0095 0.0009 0.006 0.0007
Bud Neck 0.0006 0.0002 0.0005 0.0003 0.0006 0.0008 0.0017 0.0001 0.0028 0.0009 0.0105 0.0007 0.0018 0.0002 0.0017 0.0023 0.0007 0.0012
Bud Periphery 0.0007 0.001 0.0005 0.0001 0.0002 0.0002 0.0014 0.0001 0.0009 0.0021 0.0013 0.0001 0.002 0.0003 0.0041 0.0011 0.0037 0.0005
Bud Site 0.0026 0.0029 0.0008 0.0003 0.0003 0.0002 0.0045 0.0011 0.0141 0.0029 0.0422 0.0001 0.0064 0.007 0.0117 0.0013 0.0023 0.0005
Cell Periphery 0.0006 0.0002 0.0002 0.0001 0.0001 0.0001 0.0007 0.0003 0.0006 0.0007 0.0005 0.0001 0.0006 0.0002 0.0007 0.0001 0.0004 0.0001
Cytoplasm 0.7789 0.9195 0.8406 0.8555 0.8545 0.8608 0.5504 0.9496 0.8409 0.7868 0.6497 0.8955 0.693 0.9166 0.7903 0.7663 0.7408 0.8736
Cytoplasmic Foci 0.0271 0.012 0.0217 0.0138 0.027 0.0187 0.0374 0.01 0.0195 0.0292 0.0536 0.0232 0.0436 0.0169 0.0373 0.046 0.0602 0.0298
Eisosomes 0.0002 0 0 0 0 0 0.0007 0 0.0001 0.0002 0.0006 0 0.0007 0 0.0001 0.0001 0.0001 0
Endoplasmic Reticulum 0.0312 0.013 0.023 0.011 0.0399 0.0251 0.0529 0.0085 0.0134 0.0149 0.0088 0.0251 0.0216 0.0076 0.0115 0.0096 0.0079 0.0114
Endosome 0.0675 0.0287 0.0567 0.0862 0.0524 0.0618 0.082 0.0151 0.033 0.08 0.0461 0.0374 0.0615 0.0191 0.0604 0.1036 0.0862 0.0428
Golgi 0.0066 0.0018 0.005 0.0019 0.0073 0.0077 0.0318 0.0022 0.0123 0.0176 0.0131 0.0044 0.0082 0.0017 0.01 0.0213 0.0127 0.0058
Lipid Particles 0.0029 0.0002 0.0005 0.0001 0.0001 0.0003 0.0105 0.0002 0.0021 0.0012 0.0183 0.0003 0.0116 0.0002 0.0021 0.003 0.0035 0.0017
Mitochondria 0.014 0.0012 0.0032 0.0018 0.0069 0.0082 0.0222 0.0004 0.0021 0.0088 0.0029 0.0026 0.0128 0.0018 0.0168 0.0046 0.0097 0.0039
None 0.0249 0.0066 0.0284 0.0056 0.0014 0.0057 0.0516 0.0073 0.0068 0.0049 0.006 0.0049 0.0389 0.0203 0.0179 0.0075 0.0123 0.0082
Nuclear Periphery 0.0042 0.0018 0.0037 0.002 0.0018 0.0013 0.0291 0.0008 0.0019 0.0032 0.0048 0.0007 0.0092 0.0008 0.0019 0.0023 0.0193 0.0012
Nucleolus 0.0006 0 0.0003 0 0 0 0.003 0 0.0002 0.0001 0.0027 0 0.0005 0.0001 0.0003 0.0001 0.0004 0.0001
Nucleus 0.0026 0.0012 0.0015 0.0019 0.0012 0.001 0.0198 0.0006 0.0015 0.002 0.0073 0.0007 0.0077 0.0012 0.0021 0.0014 0.0063 0.0011
Peroxisomes 0.0019 0.0001 0.0002 0 0.0002 0.0001 0.0093 0 0.0014 0.0007 0.0452 0.0001 0.0196 0.0002 0.0055 0.003 0.0055 0.0044
Punctate Nuclear 0.0013 0.0002 0.0005 0.0002 0.0001 0.0002 0.0318 0.0001 0.001 0.0006 0.0163 0.0002 0.0033 0.0005 0.0021 0.0008 0.0064 0.0011
Vacuole 0.0101 0.0059 0.0064 0.0141 0.0032 0.0048 0.0082 0.002 0.0036 0.0162 0.0036 0.0021 0.011 0.003 0.0064 0.0085 0.0054 0.0029
Vacuole Periphery 0.0025 0.0013 0.0019 0.0044 0.0014 0.002 0.0037 0.0003 0.0008 0.0026 0.0011 0.0008 0.0031 0.0005 0.0017 0.0028 0.0028 0.0011

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.1122 12.6135 13.1037 21.4235 13.7207 9.8716 17.1109 22.6671 19.9946 20.9795
Translational Efficiency 1.559 1.5126 1.2401 0.788 1.1562 1.8595 1.1377 0.9089 1.097 1.094

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Putative RNA binding protein; localizes to stress granules induced by glucose deprivation; interacts with Rbg1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (95%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Rie1

Rie1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rie1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available