ORF | |
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Human Ortholog | |
Description | Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF HUA1/YGR268C |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Bud | 1 | 0 | 2 | 1 | 0 | 2 | 4 | 4 | 1 | 0 | 1 | 8 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 2 | 0 | 2 | 2 | 3 | 1 | 2 | 8 | 3 | 3 | 3 | 2 | 2 | 6 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 454 | 325 | 201 | 228 | 255 | 380 | 388 | 506 | 413 | 348 | 291 | 360 | 288 | 477 | 536 | 47 | 33 | 68 | 452 | 303 | 194 | 192 | 377 | 392 |
Endoplasmic Reticulum | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 3 | 2 | 7 | 7 | 1 | 3 | 2 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 3 | 4 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 4 | 2 | 0 | 1 | 2 | 1 |
Golgi | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 2 | 5 |
Mitochondria | 3 | 4 | 7 | 3 | 3 | 29 | 54 | 86 | 154 | 124 | 134 | 155 | 0 | 0 | 2 | 1 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 0 |
Nucleus | 2 | 2 | 1 | 3 | 2 | 1 | 3 | 8 | 8 | 4 | 5 | 7 | 3 | 3 | 1 | 1 | 1 | 2 | 1 | 0 | 0 | 2 | 1 | 3 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 2 | 1 | 1 | 1 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 5 | 2 | 1 | 0 | 6 | 20 | 17 | 41 | 10 | 13 | 3 | 32 | 0 | 1 | 2 | 4 | 8 | 9 | 2 | 5 | 1 | 1 | 4 | 3 |
Unique Cell Count | 464 | 329 | 206 | 232 | 263 | 412 | 429 | 601 | 519 | 438 | 394 | 472 | 290 | 482 | 544 | 48 | 35 | 72 | 472 | 321 | 201 | 202 | 398 | 418 |
Labelled Cell Count | 469 | 336 | 213 | 239 | 269 | 435 | 470 | 654 | 598 | 496 | 445 | 575 | 295 | 489 | 551 | 55 | 42 | 81 | 472 | 321 | 201 | 202 | 398 | 418 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.8 | 9.5 | 5.9 | 8.3 | 8.5 | 7.2 | 6.1 | 5.9 | 4.9 | 5.2 | 4.8 | 4.7 | 11.2 | 10.6 | 10.3 | 12.5 | 12.4 | 11.9 | 10.3 | 11.2 | 11.5 |
Std Deviation (1e-4) | 1.1 | 1.3 | 1.1 | 1.4 | 1.5 | 1.2 | 1.4 | 1.2 | 1.0 | 1.4 | 0.9 | 1.0 | 1.6 | 1.5 | 1.3 | 2.1 | 2.0 | 1.7 | 1.7 | 2.0 | 2.0 |
Intensity Change (Log2) | – | – | – | 0.5 | 0.54 | 0.29 | 0.07 | 0.0 | -0.24 | -0.18 | -0.28 | -0.32 | 0.93 | 0.86 | 0.81 | 1.09 | 1.08 | 1.03 | 0.82 | 0.94 | 0.97 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.5 | -0.4 | -2.6 | -3.2 | -5.0 | -6.1 | -6.0 | -7.2 | -6.8 | 1.6 | 1.4 | 0.9 | 0.1 | -1.1 | -1.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 1.8 | 3.7 | 4.2 | 7.7 | 7.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 2.8 | 2.5 | 3.5 | 0 | 0 | 0 | 3.5 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 8.5307 | 11.2822 | 10.0063 | 9.2971 | 9.1829 | 9.9924 | 6.9481 | 9.6378 | 8.2774 | 7.9756 | 9.1124 | 8.4714 | 11.0916 | 13.3436 | 12.3609 | 11.557 | 11.8114 | 11.6269 |
Actin | 0.0592 | 0.0003 | 0.0303 | 0.0003 | 0.0647 | 0.0059 | 0.0519 | 0.0002 | 0.0436 | 0.0227 | 0.0001 | 0.0127 | 0.0056 | 0.0002 | 0.0007 | 0.008 | 0.0001 | 0.0011 |
Bud | 0.0012 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0.0003 | 0.0008 | 0 | 0.0004 | 0.0008 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0003 |
Bud Neck | 0.0023 | 0.0002 | 0.0013 | 0.0005 | 0.0006 | 0.0022 | 0.0021 | 0.0001 | 0.0007 | 0.0012 | 0.0003 | 0.0088 | 0.0005 | 0.0001 | 0.0004 | 0.0014 | 0.0006 | 0.0017 |
Bud Periphery | 0.0012 | 0 | 0.0008 | 0 | 0.0003 | 0.0001 | 0.0007 | 0 | 0.0005 | 0.0007 | 0 | 0.0003 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 |
Bud Site | 0.0038 | 0.0002 | 0.0015 | 0.0001 | 0.0004 | 0.0005 | 0.0044 | 0.0001 | 0.0024 | 0.0032 | 0 | 0.0002 | 0.0002 | 0.0004 | 0.0002 | 0.0003 | 0 | 0.0001 |
Cell Periphery | 0.0004 | 0 | 0.0002 | 0 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.824 | 0.9885 | 0.9089 | 0.9585 | 0.89 | 0.9323 | 0.7172 | 0.9913 | 0.8553 | 0.8699 | 0.9876 | 0.903 | 0.9318 | 0.9872 | 0.9732 | 0.9585 | 0.9796 | 0.9426 |
Cytoplasmic Foci | 0.0159 | 0.0005 | 0.0072 | 0.0131 | 0.0018 | 0.0067 | 0.0425 | 0.0007 | 0.0258 | 0.0368 | 0.0009 | 0.0114 | 0.0103 | 0.0006 | 0.0061 | 0.0061 | 0.0008 | 0.0054 |
Eisosomes | 0.0003 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0.0005 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0124 | 0.0005 | 0.0032 | 0.0018 | 0.0015 | 0.0024 | 0.0086 | 0.0005 | 0.002 | 0.0008 | 0.0008 | 0.0034 | 0.0044 | 0.0006 | 0.0009 | 0.0014 | 0.0006 | 0.0031 |
Endosome | 0.0248 | 0.0001 | 0.0081 | 0.0032 | 0.0019 | 0.0078 | 0.076 | 0.0001 | 0.0157 | 0.0103 | 0.0003 | 0.0122 | 0.0086 | 0.0001 | 0.0009 | 0.0016 | 0.0002 | 0.005 |
Golgi | 0.0064 | 0 | 0.0032 | 0.0003 | 0.0015 | 0.0015 | 0.0213 | 0 | 0.0153 | 0.0165 | 0 | 0.0133 | 0.0008 | 0 | 0.0002 | 0.0004 | 0 | 0.0004 |
Lipid Particles | 0.0048 | 0 | 0.0027 | 0.0027 | 0.0004 | 0.0011 | 0.0106 | 0 | 0.004 | 0.003 | 0 | 0.0015 | 0.0012 | 0 | 0.0006 | 0.0001 | 0 | 0.0002 |
Mitochondria | 0.0062 | 0.0001 | 0.0079 | 0.0001 | 0.0012 | 0.0011 | 0.0038 | 0 | 0.0094 | 0.0036 | 0 | 0.0067 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0005 |
None | 0.0057 | 0.001 | 0.0019 | 0.0057 | 0.001 | 0.0018 | 0.0112 | 0.0013 | 0.0038 | 0.0012 | 0.0006 | 0.0009 | 0.0109 | 0.0008 | 0.0016 | 0.003 | 0.0005 | 0.0036 |
Nuclear Periphery | 0.0087 | 0.0008 | 0.0032 | 0.0018 | 0.0024 | 0.0122 | 0.0116 | 0.0007 | 0.0014 | 0.0017 | 0.0008 | 0.0015 | 0.0045 | 0.0008 | 0.0012 | 0.0013 | 0.0014 | 0.0057 |
Nucleolus | 0.0002 | 0 | 0.0002 | 0.0001 | 0 | 0.0002 | 0.0004 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0.0002 |
Nucleus | 0.0121 | 0.0075 | 0.0077 | 0.0081 | 0.0298 | 0.0177 | 0.0145 | 0.0045 | 0.0073 | 0.0111 | 0.008 | 0.0159 | 0.0142 | 0.0085 | 0.0124 | 0.0152 | 0.0153 | 0.0251 |
Peroxisomes | 0.0033 | 0 | 0.008 | 0.0001 | 0.0004 | 0.0006 | 0.0099 | 0 | 0.009 | 0.0098 | 0 | 0.0065 | 0.0013 | 0 | 0.0003 | 0.0005 | 0 | 0.0004 |
Punctate Nuclear | 0.0037 | 0.0002 | 0.0025 | 0.0027 | 0.0008 | 0.004 | 0.0038 | 0.0003 | 0.0015 | 0.0058 | 0.0003 | 0.0007 | 0.0041 | 0.0002 | 0.001 | 0.0016 | 0.0005 | 0.0034 |
Vacuole | 0.0025 | 0.0001 | 0.0004 | 0.0006 | 0.0005 | 0.0006 | 0.0063 | 0.0001 | 0.0007 | 0.0005 | 0.0001 | 0.0005 | 0.0007 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0007 |
Vacuole Periphery | 0.0009 | 0 | 0.0002 | 0.0002 | 0.0001 | 0.001 | 0.0017 | 0 | 0.0005 | 0.0002 | 0 | 0.0003 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0.0002 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | – | – | – | – | – | – | – | – | – | – |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | – |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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