Standard name
Human Ortholog
Description Biotin synthase; catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud 0 0 0.06 0 0 0 0.1 0.08 0 0 0.07 0.07 0 0 0 0 0.12 0.08 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.07 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.13 0.17 0.11 0 0 0 0 0 0
Cytoplasm 0.18 0 0.08 0.15 0.19 0.2 0.22 0.27 0.32 0.37 0.31 0.29 0.86 0.91 0.95 0 0.12 0.1 0 0 0 0.11 0.14 0.18
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0.11 0.06 0 0.12 0 0 0 0 0 0 0 0 0 0 0.13 0.21 0.09 0.07 0.05 0.05 0.06 0.06 0.07
Golgi 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.28 0 0.13 0 0.06 0 0 0 0
Mitochondria 0.82 0.81 0.74 0.74 0.53 0.81 0.8 0.68 0.75 0.68 0.78 0.76 0.06 0 0 0.17 0 0.06 0.64 0.74 0.81 0.61 0.57 0.52
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0.09 0.09 0.11 0.09 0 0 0 0 0 0 0 0 0 0 0.12 0.25 0.18 0 0 0 0 0 0
SpindlePole 0 0 0.14 0.1 0.12 0.07 0.07 0.11 0.06 0.07 0 0.07 0 0 0 0.12 0.12 0.07 0 0 0 0 0 0
Vac/Vac Membrane 0 0.13 0.07 0 0.08 0 0 0 0 0 0 0 0 0 0 0.2 0.12 0.22 0 0 0 0.06 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 8 6 6 1 0 1 1 0 0 0 3 1 1 0 1 1 3 5 7 2 4 11 14
Bud 3 4 14 9 10 13 34 28 12 12 16 14 0 2 0 1 3 8 2 1 0 2 4 7
Bud Neck 1 0 3 0 4 1 2 7 1 8 1 1 2 1 1 0 0 0 0 1 0 2 2 4
Bud Site 0 2 3 1 0 0 1 8 9 1 1 0 0 0 0 0 0 3 – – – – – –
Cell Periphery 0 19 6 10 4 3 5 3 2 0 1 3 2 1 1 8 4 12 0 0 1 0 1 1
Cytoplasm 23 7 20 32 44 59 74 98 91 114 71 54 113 168 162 1 3 10 5 0 4 20 34 50
Endoplasmic Reticulum 3 9 4 3 5 2 0 1 1 2 0 0 3 1 0 2 0 5 7 1 1 1 3 1
Endosome 0 27 14 7 28 4 4 4 0 1 0 0 2 8 2 8 5 9 13 14 17 10 16 18
Golgi 1 12 2 2 2 0 0 1 0 0 0 0 3 0 0 17 1 14 6 18 4 2 1 2
Mitochondria 108 208 180 153 124 235 273 249 217 210 178 143 8 2 1 10 1 6 118 215 258 116 145 145
Nucleus 0 1 5 0 5 2 6 5 5 3 1 0 0 0 0 2 0 6 0 0 0 0 1 0
Nuclear Periphery 0 9 0 0 0 0 2 1 3 1 1 0 0 0 0 1 1 2 1 1 3 3 1 0
Nucleolus 2 4 0 0 1 2 1 1 1 0 1 0 0 0 0 0 1 3 0 1 1 1 1 1
Peroxisomes 1 22 22 22 20 3 3 7 2 3 1 0 1 0 5 7 6 19 4 11 12 5 4 2
SpindlePole 2 6 34 20 29 20 24 40 17 23 2 14 0 2 0 7 3 7 4 1 2 5 8 8
Vac/Vac Membrane 5 33 16 0 18 12 9 10 8 6 8 8 0 5 2 12 3 23 7 12 3 11 9 11
Unique Cell Count 131 257 243 207 232 291 342 365 288 309 229 188 131 184 171 60 24 105 186 291 321 191 257 278
Labelled Cell Count 150 371 329 265 295 356 439 464 369 384 282 240 135 191 174 77 32 130 186 291 321 191 257 278


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 6.6 5.3 4.9 5.2 4.0 3.7 4.2 3.5 3.5 3.2 3.5 4.9 5.3 5.3 7.9 7.2 7.3 5.1 5.2 5.4
Std Deviation (1e-4) 0.7 3.2 1.2 1.6 1.8 1.2 1.5 1.9 1.5 1.8 1.1 1.0 1.4 1.4 1.9 2.2 2.1 2.3 1.9 2.3 2.7
Intensity Change (Log2) – – – -0.09 -0.02 -0.4 -0.53 -0.32 -0.59 -0.58 -0.7 -0.58 -0.1 0.01 0.02 0.58 0.46 0.46 -0.05 -0.01 0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.3 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud -0.7 -0.7 0 0 0 0 0 0 0 0 -2.5 -3.2 0 0 0.7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.3 0 0 0 0 0 0 0 0 0 0 0 0 0 3.5
Cytoplasm 2.4 3.4 3.9 4.4 5.7 6.6 7.8 6.3 5.6 15.0 17.1 17.5 0 0 0.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -1.2 2.4 0 0 0 0 0 0 0 -1.9 -0.7 -2.4 0 0 1.0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 -4.7 0 0 0 0 0 0 0 -12.5 -15.1 -14.8 -8.2 -7.0 -11.7
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.6 -0.2 0 0 0 0 0 0 0 -3.2 -4.2 -2.5 0.6 0 2.4
SpindlePole -1.4 -0.5 0 0 0 0 0 0 0 -4.5 -4.8 -5.1 -0.5 0 -1.9
Vacuole -3.8 0.5 -1.3 -2.3 -2.3 -2.1 -2.8 -1.5 -1.0 -3.0 -1.8 -2.7 3.2 0 4.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.8971 6.7119 5.7962 5.2639 5.3491 5.8873 4.7415 4.0984 4.9708 3.825 3.4703 4.2162 6.2311 6.6082 6.024 5.661 5.4292 5.4129
Actin 0.0268 0.0067 0.0097 0.0103 0.0178 0.0098 0.0206 0.0069 0.0103 0.0013 0.0015 0.0026 0.0502 0.0093 0.0318 0.0047 0.0018 0.0105
Bud 0.0004 0.0007 0.0001 0.0024 0.0003 0.0003 0.0013 0.0021 0.0015 0.0001 0.0003 0.0002 0.0009 0.0009 0.0004 0.0012 0.0001 0.0001
Bud Neck 0.0021 0.0003 0.0001 0.0007 0.0004 0.0002 0.0014 0.0006 0.0005 0.0002 0.0003 0.0003 0.0008 0.0004 0.0022 0.0005 0.0002 0.0002
Bud Periphery 0.0007 0.0009 0.0003 0.0097 0.0006 0.0005 0.0032 0.0034 0.0021 0.0004 0.0009 0.0009 0.0013 0.0014 0.0006 0.0048 0.0003 0.0004
Bud Site 0.0058 0.0089 0.0006 0.0018 0.001 0.0002 0.0045 0.0146 0.0142 0.0004 0.0002 0.0003 0.0029 0.0039 0.0013 0.0018 0.0003 0.0002
Cell Periphery 0.0005 0.0002 0.0004 0.0003 0.0001 0.0001 0.0007 0.0008 0.0004 0.0001 0.0001 0.0001 0.0004 0.0003 0.0001 0.002 0.0001 0.0001
Cytoplasm 0.0008 0.0005 0.0003 0.0018 0.0003 0.0001 0.0029 0.0014 0.0008 0.0001 0.0016 0.0011 0.0003 0.0006 0.0005 0.0002 0.0001 0.0001
Cytoplasmic Foci 0.0151 0.0098 0.003 0.0052 0.0101 0.0083 0.0192 0.0142 0.0295 0.013 0.0218 0.0177 0.0062 0.0063 0.0124 0.0043 0.0051 0.0024
Eisosomes 0.0014 0.0002 0.0019 0.0002 0.0003 0.0005 0.0004 0.0003 0.0003 0.0001 0.0001 0.0002 0.0006 0.0002 0.0003 0.0003 0.0001 0.0002
Endoplasmic Reticulum 0.0014 0.0001 0.0006 0.0007 0.0002 0.0001 0.0005 0.0004 0.0002 0 0.0001 0.0001 0.0005 0.0003 0.0004 0.0001 0.0002 0.0002
Endosome 0.0232 0.023 0.0063 0.0173 0.0062 0.0072 0.0307 0.0215 0.019 0.0109 0.0108 0.0161 0.0181 0.021 0.019 0.0073 0.0278 0.0051
Golgi 0.0404 0.0376 0.0127 0.0173 0.0172 0.0277 0.0227 0.0407 0.0182 0.0171 0.02 0.0278 0.0346 0.0428 0.0344 0.0104 0.0305 0.0219
Lipid Particles 0.0316 0.0035 0.0103 0.0108 0.0057 0.0108 0.0258 0.0104 0.0068 0.0141 0.0048 0.0139 0.0319 0.0057 0.0092 0.0077 0.0173 0.0085
Mitochondria 0.8023 0.8789 0.9301 0.8883 0.8687 0.9109 0.7918 0.8278 0.8267 0.8912 0.901 0.8683 0.8113 0.881 0.8686 0.9265 0.8713 0.9263
None 0.0027 0.0001 0.0002 0.0006 0.0007 0.0001 0.0101 0.0013 0.0009 0.0001 0.0217 0.0071 0.0005 0.0002 0.0002 0.0001 0.0001 0.0001
Nuclear Periphery 0.0021 0.0001 0.0001 0.0014 0.0018 0 0.0014 0.0013 0.0002 0 0.0002 0 0.0063 0.0003 0.0001 0.0001 0.0001 0.0002
Nucleolus 0.0011 0.0001 0 0.0003 0.0014 0 0.001 0.0013 0.0007 0.0001 0.0001 0.0001 0.0009 0.0003 0.0001 0.0009 0.0002 0.0001
Nucleus 0.0005 0.0001 0.0001 0.0005 0.001 0 0.0006 0.0004 0.0006 0 0.0001 0.0001 0.0009 0.0003 0.0001 0.0003 0.0001 0.0001
Peroxisomes 0.0355 0.0231 0.0215 0.0241 0.0444 0.0194 0.0503 0.0446 0.061 0.0495 0.0067 0.0407 0.019 0.0191 0.015 0.018 0.0393 0.0198
Punctate Nuclear 0.0022 0.0001 0.0001 0.0008 0.0201 0.002 0.001 0.0002 0.0017 0.0001 0.001 0.0005 0.0005 0.0001 0.0006 0 0.0001 0
Vacuole 0.0013 0.0018 0.0006 0.0034 0.0004 0.0005 0.0067 0.0037 0.0024 0.0004 0.0023 0.0007 0.003 0.0026 0.0012 0.0048 0.0018 0.0006
Vacuole Periphery 0.0018 0.0035 0.0009 0.0022 0.0012 0.0012 0.003 0.0021 0.0019 0.0006 0.0045 0.0011 0.0089 0.0032 0.0018 0.004 0.003 0.0029

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 126.6763 35.8523 21.413 21.2675 30.4386 82.1229 22.1038 13.4267 15.419 17.2659
Translational Efficiency 1.2382 1.2822 1.0077 1.2138 1.2752 1.6606 1.0403 1.4786 1.0375 1.1713

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Biotin synthase; catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant
Localization
Cell Percentages mitochondrion (78%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Bio2

Bio2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bio2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available