ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein co-localizes with clathrin-coated vesicles; YHL017W has a paralog, PTM1, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.06 0 0 0 0 0 0 0 0 0 0 0.14 0.18 0.1 0.06 0.06 0 0 0 0 0.05 0.06
Bud 0 0 0 0 0 0 0.06 0.06 0.09 0.08 0.13 0.09 0 0 0 0.08 0.06 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.06 0 0.05 0.05 0 0 0 0 0 0 0 0 0.07 0.08 0.14 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0.05 0.11 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0 0 0 0 0 0 0 0
Endosome 0.14 0 0.21 0.2 0.28 0.06 0.12 0.22 0.05 0 0 0 0.16 0.12 0.14 0.17 0.08 0.23 0.23 0.21 0.2 0.17 0.17
Golgi 0.12 0 0.07 0.07 0.06 0 0 0 0 0 0 0 0.29 0.48 0.44 0.28 0.39 0.33 0.29 0.43 0.45 0.51 0.52
Mitochondria 0.8 0.83 0.75 0.77 0.61 0.87 0.8 0.62 0.86 0.89 0.91 0.87 0.38 0.24 0.18 0 0 0.18 0.21 0.06 0.12 0.11 0.09
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0.06 0 0 0.05 0 0 0 0 0 0 0.08 0 0 0 0 0 0
Peroxisomes 0 0 0.18 0.13 0.23 0.12 0.12 0.21 0.12 0.08 0.05 0.09 0.06 0 0 0.14 0.08 0.15 0 0.24 0.15 0.13 0.14
SpindlePole 0 0 0.07 0 0.08 0 0.09 0.16 0.09 0.07 0.06 0.09 0.16 0.08 0.09 0 0.06 0 0 0 0 0 0
Vac/Vac Membrane 0.08 0.11 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0.15 0.06 0.08 0 0.12 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 2 3 6 6 1 1 3 4 2 1 2 65 70 36 4 2 1 0 18 22 44 49
Bud 2 0 22 17 22 26 63 58 74 84 80 76 14 6 10 6 2 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 5 10 8 9 24 28 14 28 0 0 0 0 0
Cell Periphery 0 0 4 4 6 2 2 8 7 2 5 3 3 5 1 3 1 0 0 0 0 0 0
Cytoplasm 2 2 12 24 38 21 22 31 22 13 16 24 19 27 27 10 4 0 1 1 1 1 0
Endoplasmic Reticulum 4 4 4 1 1 1 1 1 4 2 1 3 14 26 16 2 0 0 0 0 1 2 2
Endosome 12 0 114 101 218 44 119 203 40 30 7 33 75 48 49 12 3 24 16 122 101 155 147
Golgi 10 1 37 36 49 0 1 7 3 0 0 1 137 189 155 20 14 36 20 256 231 465 460
Mitochondria 67 29 414 396 471 638 820 575 727 926 568 737 182 94 65 3 1 19 15 34 60 99 80
Nucleus 0 1 0 0 1 1 2 8 4 2 6 2 2 2 0 0 0 1 0 0 0 0 0
Nuclear Periphery 0 0 1 3 1 3 5 4 5 11 3 1 3 1 0 2 0 0 0 0 1 1 0
Nucleolus 0 0 3 5 14 19 63 38 25 47 20 25 4 0 2 3 3 0 0 1 0 0 0
Peroxisomes 3 0 101 69 177 87 125 191 102 88 31 78 29 5 5 10 3 16 2 144 78 120 124
SpindlePole 2 0 37 13 60 29 90 147 76 70 40 76 75 32 31 3 2 2 2 2 4 6 3
Vac/Vac Membrane 7 4 13 11 10 12 29 24 17 14 12 10 39 25 52 4 3 3 8 2 4 6 8
Unique Cell Count 84 35 551 516 770 733 1020 926 847 1042 627 846 477 392 353 71 36 109 71 591 511 909 884
Labelled Cell Count 111 43 765 686 1079 894 1351 1307 1134 1320 804 1099 661 530 449 82 38 109 71 591 511 909 884


Late Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.8 5.1 6.1 7.0 6.8 5.3 4.8 5.5 4.7 4.4 4.5 4.4 8.0 8.0 7.6 7.6 9.4 8.0 8.1 8.1
Std Deviation (1e-4) 1.0 0.7 1.0 1.4 1.8 1.4 1.3 1.4 1.4 1.1 1.3 1.2 0.8 0.9 0.9 1.6 1.7 1.2 1.2 1.3
Intensity Change (Log2) 0.2 0.18 -0.19 -0.35 -0.15 -0.36 -0.45 -0.44 -0.47 0.4 0.4 0.33 0.33 0.63 0.41 0.41 0.41


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 8.4 9.8 7.0 0 0
Bud -0.6 -1.1 -0.4 0 1.9 0 0 0 0 -0.9 -2.2 -0.9 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.2 2.6 0.8 0 1.3 0.5 -1.4 0.4 0.8 1.7 3.6 3.9 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 2.7 5.1 3.8 0 0
Endosome -0.5 3.1 -7.9 0 0.6 0 0 0 0 -2.1 -3.4 -2.6 -0.7 -1.8
Golgi 0.2 -0.3 -7.1 0 -6.5 0 0 0 0 9.4 14.7 13.3 5.9 0
Mitochondria 0.6 -5.3 5.5 0 -5.2 0 0 0 0 -12.0 -15.5 -16.7 -12.0 -9.2
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 2.0 2.8 0 4.0 0 0 0 0 0 0 0 0 0
Peroxisomes -2.2 2.0 -3.2 0 1.1 0 0 0 0 -5.9 -8.2 -7.7 -0.9 -1.5
SpindlePole -3.2 0.7 -2.2 0 5.2 0 0 0 0 4.6 0.8 1.1 0 0
Vacuole -0.3 -1.5 -0.9 0.6 0.3 -0.4 -1.5 -0.5 -1.7 4.2 3.1 7.0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.0413 4.0406 3.7971 3.3322 4.1907 3.6194 1.8111 2.9329 2.6332 2.2057 1.8279 2.5355 1.5496 2.1145 2.0495 2.3529 1.6308 1.7912
Actin 0.0294 0.0136 0.0103 0.0086 0.0009 0.014 0.0701 0.017 0.0393 0.0104 0.0108 0.0281 0.0119 0.0068 0.0189 0.0213 0.0003 0.021
Bud 0.0002 0.0003 0.0003 0.0001 0 0.0009 0.0004 0.0007 0.0002 0.0002 0.0001 0.0001 0.0002 0.0002 0.0001 0.0002 0.0001 0.0001
Bud Neck 0.0005 0.0004 0.0005 0.0004 0.0002 0.0009 0.0008 0.001 0.0005 0.0006 0.0003 0.0009 0.0007 0.0005 0.001 0.0005 0.0002 0.0006
Bud Periphery 0.0002 0.0002 0.0002 0.0001 0 0.0009 0.0005 0.001 0.0001 0.0002 0.0001 0.0001 0.0002 0.0001 0.0001 0.0002 0 0.0001
Bud Site 0.0006 0.0011 0.0007 0.0004 0.0001 0.0007 0.0022 0.0189 0.0005 0.0003 0.0002 0.0003 0.0029 0.0008 0.0004 0.001 0.0001 0.0002
Cell Periphery 0.0001 0 0.0001 0 0 0.0002 0.0001 0.0005 0.0001 0 0 0 0.0002 0.0001 0.0001 0.0001 0 0.0001
Cytoplasm 0.0012 0.0067 0.0041 0.0009 0.0008 0.0037 0.0019 0.0093 0.0011 0.0008 0.0007 0.0009 0.0017 0.0039 0.0038 0.0013 0.0007 0.001
Cytoplasmic Foci 0.1535 0.1884 0.1513 0.1498 0.0954 0.1074 0.1343 0.1617 0.1139 0.1464 0.1018 0.1231 0.1405 0.1388 0.1019 0.0928 0.1147 0.0943
Eisosomes 0.0001 0 0 0 0 0.0001 0.0002 0.0004 0.0016 0 0 0.0001 0.0001 0.0001 0.0002 0.0001 0 0.0001
Endoplasmic Reticulum 0.0004 0.0003 0.0006 0.0002 0.0003 0.0007 0.0017 0.0005 0.0005 0.0002 0.0002 0.0002 0.0008 0.0007 0.0008 0.0027 0.0003 0.0004
Endosome 0.2488 0.2469 0.2457 0.2218 0.3318 0.1815 0.2094 0.1663 0.2086 0.2377 0.1583 0.1233 0.2118 0.2036 0.19 0.1904 0.2977 0.1752
Golgi 0.38 0.4115 0.4682 0.4281 0.4098 0.4758 0.3814 0.511 0.5334 0.4861 0.5066 0.5393 0.3873 0.4832 0.5455 0.4587 0.4812 0.5442
Lipid Particles 0.045 0.0315 0.0197 0.0482 0.0602 0.0353 0.0839 0.0118 0.0221 0.0502 0.0527 0.0475 0.1242 0.0526 0.037 0.0347 0.0844 0.0515
Mitochondria 0.0412 0.0163 0.0352 0.0323 0.0272 0.0669 0.0151 0.0386 0.0148 0.0077 0.0554 0.0247 0.0297 0.0458 0.0262 0.057 0.0055 0.0315
None 0.0002 0.0002 0.0004 0.0001 0.0001 0.0086 0.001 0.0019 0.0037 0.0001 0.0001 0.0001 0.0005 0.0002 0.0113 0.0003 0 0.0001
Nuclear Periphery 0.0001 0.0001 0.0004 0.0001 0.0001 0.0023 0.0016 0.0001 0.0009 0.0001 0.0001 0.0001 0.0008 0.0001 0.0005 0.0042 0.0001 0.0001
Nucleolus 0.0001 0.0001 0.0001 0.0001 0 0.0003 0.0013 0.0001 0.0003 0 0 0 0.0012 0.0003 0.0001 0.0001 0 0
Nucleus 0.0001 0.0001 0.0001 0 0 0.0005 0.0008 0.0001 0.0002 0 0 0 0.0005 0.0001 0.0003 0.0004 0 0
Peroxisomes 0.0945 0.0786 0.0579 0.1066 0.0685 0.0931 0.0882 0.0557 0.0549 0.0566 0.1106 0.1095 0.0728 0.0573 0.0574 0.1303 0.0113 0.0763
Punctate Nuclear 0.0005 0.0007 0.0004 0.0003 0.0001 0.0027 0.0017 0.0002 0.0008 0.0002 0.0001 0.0003 0.0014 0.0004 0.0008 0.0014 0.0001 0.0002
Vacuole 0.0017 0.0018 0.0021 0.0008 0.0016 0.0017 0.0019 0.0018 0.0011 0.001 0.0009 0.0005 0.0064 0.0024 0.0013 0.001 0.0015 0.0012
Vacuole Periphery 0.0018 0.0012 0.0018 0.001 0.0027 0.0019 0.0015 0.0011 0.0015 0.0013 0.001 0.0008 0.0042 0.0018 0.0022 0.0014 0.0019 0.0017

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 25.8772 16.0386 18.7675 23.1244 19.6832 18.6213 20.2708 20.6274 21.3149 28.1022
Translational Efficiency 0.5479 0.7599 0.6672 0.465 0.6166 0.8273 0.6095 0.4476 0.5911 0.5062

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein co-localizes with clathrin-coated vesicles; YHL017W has a paralog, PTM1, that arose from the whole genome duplication
Localization
Cell Percentages Golgi (15%)
Cell Cycle Regulation No
Subcompartmental Group N/A

YHL017W

YHL017W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yhl017w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available