ORF | |
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Human Ortholog | |
Description | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein co-localizes with clathrin-coated vesicles; YHL017W has a paralog, PTM1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 2 | 3 | 6 | 6 | 1 | 1 | 3 | 4 | 2 | 1 | 2 | 65 | 70 | 36 | – | 4 | 2 | 1 | 0 | 18 | 22 | 44 | 49 |
Bud | 2 | 0 | 22 | 17 | 22 | 26 | 63 | 58 | 74 | 84 | 80 | 76 | 14 | 6 | 10 | – | 6 | 2 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 5 | 10 | 8 | 9 | 24 | 28 | 14 | 28 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 4 | 4 | 6 | 2 | 2 | 8 | 7 | 2 | 5 | 3 | 3 | 5 | 1 | – | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 2 | 12 | 24 | 38 | 21 | 22 | 31 | 22 | 13 | 16 | 24 | 19 | 27 | 27 | – | 10 | 4 | 0 | 1 | 1 | 1 | 1 | 0 |
Endoplasmic Reticulum | 4 | 4 | 4 | 1 | 1 | 1 | 1 | 1 | 4 | 2 | 1 | 3 | 14 | 26 | 16 | – | 2 | 0 | 0 | 0 | 0 | 1 | 2 | 2 |
Endosome | 12 | 0 | 114 | 101 | 218 | 44 | 119 | 203 | 40 | 30 | 7 | 33 | 75 | 48 | 49 | – | 12 | 3 | 24 | 16 | 122 | 101 | 155 | 147 |
Golgi | 10 | 1 | 37 | 36 | 49 | 0 | 1 | 7 | 3 | 0 | 0 | 1 | 137 | 189 | 155 | – | 20 | 14 | 36 | 20 | 256 | 231 | 465 | 460 |
Mitochondria | 67 | 29 | 414 | 396 | 471 | 638 | 820 | 575 | 727 | 926 | 568 | 737 | 182 | 94 | 65 | – | 3 | 1 | 19 | 15 | 34 | 60 | 99 | 80 |
Nucleus | 0 | 1 | 0 | 0 | 1 | 1 | 2 | 8 | 4 | 2 | 6 | 2 | 2 | 2 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 1 | 3 | 1 | 3 | 5 | 4 | 5 | 11 | 3 | 1 | 3 | 1 | 0 | – | 2 | 0 | 0 | 0 | 0 | 1 | 1 | 0 |
Nucleolus | 0 | 0 | 3 | 5 | 14 | 19 | 63 | 38 | 25 | 47 | 20 | 25 | 4 | 0 | 2 | – | 3 | 3 | 0 | 0 | 1 | 0 | 0 | 0 |
Peroxisomes | 3 | 0 | 101 | 69 | 177 | 87 | 125 | 191 | 102 | 88 | 31 | 78 | 29 | 5 | 5 | – | 10 | 3 | 16 | 2 | 144 | 78 | 120 | 124 |
SpindlePole | 2 | 0 | 37 | 13 | 60 | 29 | 90 | 147 | 76 | 70 | 40 | 76 | 75 | 32 | 31 | – | 3 | 2 | 2 | 2 | 2 | 4 | 6 | 3 |
Vac/Vac Membrane | 7 | 4 | 13 | 11 | 10 | 12 | 29 | 24 | 17 | 14 | 12 | 10 | 39 | 25 | 52 | – | 4 | 3 | 3 | 8 | 2 | 4 | 6 | 8 |
Unique Cell Count | 84 | 35 | 551 | 516 | 770 | 733 | 1020 | 926 | 847 | 1042 | 627 | 846 | 477 | 392 | 353 | 71 | 36 | 109 | 71 | 591 | 511 | 909 | 884 | |
Labelled Cell Count | 111 | 43 | 765 | 686 | 1079 | 894 | 1351 | 1307 | 1134 | 1320 | 804 | 1099 | 661 | 530 | 449 | 82 | 38 | 109 | 71 | 591 | 511 | 909 | 884 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.8 | 5.1 | 6.1 | 7.0 | 6.8 | 5.3 | 4.8 | 5.5 | 4.7 | 4.4 | 4.5 | 4.4 | 8.0 | 8.0 | 7.6 | – | 7.6 | 9.4 | 8.0 | 8.1 | 8.1 |
Std Deviation (1e-4) | 1.0 | 0.7 | 1.0 | 1.4 | 1.8 | 1.4 | 1.3 | 1.4 | 1.4 | 1.1 | 1.3 | 1.2 | 0.8 | 0.9 | 0.9 | – | 1.6 | 1.7 | 1.2 | 1.2 | 1.3 |
Intensity Change (Log2) | – | – | – | 0.2 | 0.18 | -0.19 | -0.35 | -0.15 | -0.36 | -0.45 | -0.44 | -0.47 | 0.4 | 0.4 | 0.33 | – | 0.33 | 0.63 | 0.41 | 0.41 | 0.41 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8.4 | 9.8 | 7.0 | – | 0 | 0 |
Bud | -0.6 | -1.1 | -0.4 | 0 | 1.9 | 0 | 0 | 0 | 0 | -0.9 | -2.2 | -0.9 | – | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Cytoplasm | 2.2 | 2.6 | 0.8 | 0 | 1.3 | 0.5 | -1.4 | 0.4 | 0.8 | 1.7 | 3.6 | 3.9 | – | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.7 | 5.1 | 3.8 | – | 0 | 0 |
Endosome | -0.5 | 3.1 | -7.9 | 0 | 0.6 | 0 | 0 | 0 | 0 | -2.1 | -3.4 | -2.6 | – | -0.7 | -1.8 |
Golgi | 0.2 | -0.3 | -7.1 | 0 | -6.5 | 0 | 0 | 0 | 0 | 9.4 | 14.7 | 13.3 | – | 5.9 | 0 |
Mitochondria | 0.6 | -5.3 | 5.5 | 0 | -5.2 | 0 | 0 | 0 | 0 | -12.0 | -15.5 | -16.7 | – | -12.0 | -9.2 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Nucleolus | 0 | 2.0 | 2.8 | 0 | 4.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Peroxisomes | -2.2 | 2.0 | -3.2 | 0 | 1.1 | 0 | 0 | 0 | 0 | -5.9 | -8.2 | -7.7 | – | -0.9 | -1.5 |
SpindlePole | -3.2 | 0.7 | -2.2 | 0 | 5.2 | 0 | 0 | 0 | 0 | 4.6 | 0.8 | 1.1 | – | 0 | 0 |
Vacuole | -0.3 | -1.5 | -0.9 | 0.6 | 0.3 | -0.4 | -1.5 | -0.5 | -1.7 | 4.2 | 3.1 | 7.0 | – | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.0413 | 4.0406 | 3.7971 | 3.3322 | 4.1907 | 3.6194 | 1.8111 | 2.9329 | 2.6332 | 2.2057 | 1.8279 | 2.5355 | 1.5496 | 2.1145 | 2.0495 | 2.3529 | 1.6308 | 1.7912 |
Actin | 0.0294 | 0.0136 | 0.0103 | 0.0086 | 0.0009 | 0.014 | 0.0701 | 0.017 | 0.0393 | 0.0104 | 0.0108 | 0.0281 | 0.0119 | 0.0068 | 0.0189 | 0.0213 | 0.0003 | 0.021 |
Bud | 0.0002 | 0.0003 | 0.0003 | 0.0001 | 0 | 0.0009 | 0.0004 | 0.0007 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 |
Bud Neck | 0.0005 | 0.0004 | 0.0005 | 0.0004 | 0.0002 | 0.0009 | 0.0008 | 0.001 | 0.0005 | 0.0006 | 0.0003 | 0.0009 | 0.0007 | 0.0005 | 0.001 | 0.0005 | 0.0002 | 0.0006 |
Bud Periphery | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0009 | 0.0005 | 0.001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0001 |
Bud Site | 0.0006 | 0.0011 | 0.0007 | 0.0004 | 0.0001 | 0.0007 | 0.0022 | 0.0189 | 0.0005 | 0.0003 | 0.0002 | 0.0003 | 0.0029 | 0.0008 | 0.0004 | 0.001 | 0.0001 | 0.0002 |
Cell Periphery | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 |
Cytoplasm | 0.0012 | 0.0067 | 0.0041 | 0.0009 | 0.0008 | 0.0037 | 0.0019 | 0.0093 | 0.0011 | 0.0008 | 0.0007 | 0.0009 | 0.0017 | 0.0039 | 0.0038 | 0.0013 | 0.0007 | 0.001 |
Cytoplasmic Foci | 0.1535 | 0.1884 | 0.1513 | 0.1498 | 0.0954 | 0.1074 | 0.1343 | 0.1617 | 0.1139 | 0.1464 | 0.1018 | 0.1231 | 0.1405 | 0.1388 | 0.1019 | 0.0928 | 0.1147 | 0.0943 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0.0002 | 0.0004 | 0.0016 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0004 | 0.0003 | 0.0006 | 0.0002 | 0.0003 | 0.0007 | 0.0017 | 0.0005 | 0.0005 | 0.0002 | 0.0002 | 0.0002 | 0.0008 | 0.0007 | 0.0008 | 0.0027 | 0.0003 | 0.0004 |
Endosome | 0.2488 | 0.2469 | 0.2457 | 0.2218 | 0.3318 | 0.1815 | 0.2094 | 0.1663 | 0.2086 | 0.2377 | 0.1583 | 0.1233 | 0.2118 | 0.2036 | 0.19 | 0.1904 | 0.2977 | 0.1752 |
Golgi | 0.38 | 0.4115 | 0.4682 | 0.4281 | 0.4098 | 0.4758 | 0.3814 | 0.511 | 0.5334 | 0.4861 | 0.5066 | 0.5393 | 0.3873 | 0.4832 | 0.5455 | 0.4587 | 0.4812 | 0.5442 |
Lipid Particles | 0.045 | 0.0315 | 0.0197 | 0.0482 | 0.0602 | 0.0353 | 0.0839 | 0.0118 | 0.0221 | 0.0502 | 0.0527 | 0.0475 | 0.1242 | 0.0526 | 0.037 | 0.0347 | 0.0844 | 0.0515 |
Mitochondria | 0.0412 | 0.0163 | 0.0352 | 0.0323 | 0.0272 | 0.0669 | 0.0151 | 0.0386 | 0.0148 | 0.0077 | 0.0554 | 0.0247 | 0.0297 | 0.0458 | 0.0262 | 0.057 | 0.0055 | 0.0315 |
None | 0.0002 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0086 | 0.001 | 0.0019 | 0.0037 | 0.0001 | 0.0001 | 0.0001 | 0.0005 | 0.0002 | 0.0113 | 0.0003 | 0 | 0.0001 |
Nuclear Periphery | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0023 | 0.0016 | 0.0001 | 0.0009 | 0.0001 | 0.0001 | 0.0001 | 0.0008 | 0.0001 | 0.0005 | 0.0042 | 0.0001 | 0.0001 |
Nucleolus | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0013 | 0.0001 | 0.0003 | 0 | 0 | 0 | 0.0012 | 0.0003 | 0.0001 | 0.0001 | 0 | 0 |
Nucleus | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0005 | 0.0008 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0005 | 0.0001 | 0.0003 | 0.0004 | 0 | 0 |
Peroxisomes | 0.0945 | 0.0786 | 0.0579 | 0.1066 | 0.0685 | 0.0931 | 0.0882 | 0.0557 | 0.0549 | 0.0566 | 0.1106 | 0.1095 | 0.0728 | 0.0573 | 0.0574 | 0.1303 | 0.0113 | 0.0763 |
Punctate Nuclear | 0.0005 | 0.0007 | 0.0004 | 0.0003 | 0.0001 | 0.0027 | 0.0017 | 0.0002 | 0.0008 | 0.0002 | 0.0001 | 0.0003 | 0.0014 | 0.0004 | 0.0008 | 0.0014 | 0.0001 | 0.0002 |
Vacuole | 0.0017 | 0.0018 | 0.0021 | 0.0008 | 0.0016 | 0.0017 | 0.0019 | 0.0018 | 0.0011 | 0.001 | 0.0009 | 0.0005 | 0.0064 | 0.0024 | 0.0013 | 0.001 | 0.0015 | 0.0012 |
Vacuole Periphery | 0.0018 | 0.0012 | 0.0018 | 0.001 | 0.0027 | 0.0019 | 0.0015 | 0.0011 | 0.0015 | 0.0013 | 0.001 | 0.0008 | 0.0042 | 0.0018 | 0.0022 | 0.0014 | 0.0019 | 0.0017 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 25.8772 | 16.0386 | 18.7675 | 23.1244 | 19.6832 | 18.6213 | 20.2708 | 20.6274 | 21.3149 | 28.1022 |
Translational Efficiency | 0.5479 | 0.7599 | 0.6672 | 0.465 | 0.6166 | 0.8273 | 0.6095 | 0.4476 | 0.5911 | 0.5062 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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