Standard name | |
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Human Ortholog | |
Description | Probable mitochondrial seryl-tRNA synthetase; mutant displays increased invasive and pseudohyphal growth |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | – | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | – | – | 1 | 3 | 1 | 2 | 0 | 1 |
Bud | 2 | – | 0 | 0 | – | 0 | 1 | 1 | 1 | 1 | 2 | 2 | 0 | 0 | 0 | – | – | – | 1 | 0 | 0 | 1 | 1 | 1 |
Bud Neck | 0 | – | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | – | – | – | 0 | 1 | 0 | 0 | 1 | 5 |
Bud Site | 0 | – | 0 | 0 | – | 0 | 0 | 5 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 0 | – | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 22 | – | 4 | 7 | – | 3 | 1 | 2 | 5 | 4 | 3 | 4 | 16 | 28 | 60 | – | – | – | 5 | 9 | 0 | 8 | 18 | 16 |
Endoplasmic Reticulum | 0 | – | 0 | 1 | – | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | – | – | – | 1 | 0 | 0 | 0 | 0 | 2 |
Endosome | 0 | – | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 5 | 25 | – | – | – | 8 | 1 | 6 | 4 | 9 | 4 |
Golgi | 0 | – | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | – | – | – | 2 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 37 | – | 8 | 12 | – | 19 | 21 | 56 | 30 | 32 | 54 | 45 | 0 | 0 | 4 | – | – | – | 53 | 20 | 42 | 21 | 35 | 50 |
Nucleus | 1 | – | 0 | 1 | – | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | – | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | – | 0 | 0 | – | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | – | 0 | 0 | – | 1 | 0 | 2 | 0 | 0 | 1 | 1 | 0 | 0 | 9 | – | – | – | 4 | 0 | 3 | 0 | 0 | 0 |
SpindlePole | 1 | – | 3 | 1 | – | 1 | 1 | 9 | 3 | 4 | 0 | 6 | 0 | 2 | 4 | – | – | – | 5 | 1 | 2 | 0 | 5 | 4 |
Vac/Vac Membrane | 0 | – | 1 | 0 | – | 1 | 1 | 3 | 1 | 1 | 0 | 0 | 1 | 4 | 15 | – | – | – | 3 | 1 | 1 | 7 | 10 | 17 |
Unique Cell Count | 53 | 16 | 17 | 21 | 23 | 62 | 33 | 38 | 56 | 50 | 19 | 38 | 103 | 93 | 41 | 63 | 49 | 90 | 111 | |||||
Labelled Cell Count | 63 | 16 | 23 | 27 | 25 | 78 | 42 | 47 | 63 | 62 | 19 | 42 | 120 | 93 | 41 | 63 | 49 | 90 | 111 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.0 | – | 3.9 | 3.3 | – | 3.2 | 3.4 | 3.3 | 3.2 | 3.6 | 2.6 | 2.8 | 5.3 | 5.3 | 5.4 | – | – | – | – | 5.7 | 5.7 |
Std Deviation (1e-4) | 0.7 | – | 0.6 | 0.6 | – | 0.6 | 0.7 | 1.4 | 1.2 | 2.4 | 0.5 | 0.7 | 1.1 | 1.1 | 1.3 | – | – | – | – | 2.4 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.24 | – | -0.28 | -0.21 | -0.23 | -0.31 | -0.13 | -0.59 | -0.5 | 0.44 | 0.45 | 0.46 | – | – | – | – | 0.54 | 0.55 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | 0 | – | – | – | – | – | – | – | – | 0 | 0 | 0 | – | – | – |
Bud Neck | 0 | – | – | – | – | – | – | – | – | 0 | 0 | 0 | – | – | – |
Bud Site | 0 | – | – | – | – | – | – | – | – | 0 | 0 | 0 | – | – | – |
Cell Periphery | 0 | – | – | – | – | – | – | – | – | 0 | 0 | 0 | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | 0 | – | – | – | – | – | – | – | – | 0 | 0 | 0 | – | – | – |
Endosome | 0 | – | – | – | – | – | – | – | – | 0 | 0 | 0 | – | – | – |
Golgi | 0 | – | – | – | – | – | – | – | – | 0 | 0 | 0 | – | – | – |
Mitochondria | 0 | – | – | – | – | – | – | – | – | 0 | 0 | 0 | – | – | – |
Nuclear Periphery | 0 | – | – | – | – | – | – | – | – | 0 | 0 | 0 | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | 0 | – | – | – | – | – | – | – | – | 0 | 0 | 0 | – | – | – |
Peroxisomes | 0 | – | – | – | – | – | – | – | – | 0 | 0 | 0 | – | – | – |
SpindlePole | 0 | – | – | – | – | – | – | – | – | 0 | 0 | 0 | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | 0 | – | – | – | – | – | – | – | – | 0 | 0 | 0 | – | – | – |
Cytoplasm | 1.0 | – | – | – | – | – | – | – | – | 3.5 | 3.3 | 2.5 | – | – | – |
Nucleus | 0 | – | – | – | – | – | – | – | – | 0 | 0 | 0 | – | – | – |
Vacuole | 0 | – | – | – | – | – | – | – | – | 0 | 0 | 0 | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasmic Foci | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Eisosomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Lipid Particles | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
None | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Punctate Nuclear | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 5.7598 | 1.4711 | 4.3665 | 7.9098 | 3.2005 | 5.7566 | 7.4372 | 7.0274 | 8.7776 | 9.3178 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
---|---|
Protein Concentration | – |
Protein Localization | – |
Gene Expression | ✘ |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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