Standard name
Human Ortholog
Description Putative metalloprotease with similarity to zinc carboxypeptidases; required for normal cell wall assembly
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.06 0.06 0.07 0.14 0.2 0.35 0.43 0.47 0.53 0.52 0.56 0.57 0.21 0.2 0.34 0.21 0.21 0.23 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0.1 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0 0 0.05 0 0 0.07 0 0.05 0.06 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.21 0.14 0.38 0.09 0.14 0.23 0.19 0.12 0.14 0.18 0.22 0.18 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.19 0.18 0.32 0.33 0.24 0.2 0.13 0.11 0.08 0.08 0.05 0.07 0.19 0.33 0.31 0.47 0.31 0.29 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.59 0.76 0.5 0.74 0.74 0.61 0.57 0.6 0.55 0.54 0.5 0.47 0.69 0.68 0.63 0.53 0.64 0.6 0.83 0.85 0.86 0.82 0.77 0.79
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 1 1 7 2 1 1 4 0 6 4 5 7 0 0 0 0 0 0 0 2 4 1 0 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 12 14 30 54 84 204 315 344 327 365 370 463 55 73 153 34 28 29 1 1 1 8 11 3
Endoplasmic Reticulum 1 4 0 0 1 4 0 0 2 1 0 2 0 1 5 7 5 6 2 0 1 1 2 2
Endosome 20 4 10 5 9 2 0 7 1 0 0 2 19 20 15 4 7 5 9 15 15 18 25 28
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 2 2 0 0 0 1
Mitochondria 44 33 174 35 60 137 139 87 87 124 142 144 2 0 2 6 5 2 7 4 9 9 11 10
Nucleus 39 42 143 127 99 117 97 81 52 58 32 55 50 122 142 75 42 37 6 3 8 8 17 7
Nuclear Periphery 4 0 3 1 4 19 7 12 4 15 6 4 0 0 0 1 0 0 2 0 8 3 4 11
Nucleolus 10 1 5 1 0 1 0 0 0 0 1 3 0 1 1 2 0 0 5 0 3 3 4 3
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 4 1 0 0 0 0 0 0 0 1 0 0 3 1 0 1 0 0 2 2 3 2
Vac/Vac Membrane 122 173 226 288 306 359 420 439 337 379 326 379 177 252 287 86 86 76 216 200 398 293 303 313
Unique Cell Count 206 228 453 388 416 584 735 737 617 699 658 809 258 368 452 161 134 127 259 237 463 357 394 397
Labelled Cell Count 254 273 602 514 564 844 982 971 816 946 882 1060 303 469 608 217 174 156 259 237 463 357 394 397


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 6.0 4.8 5.4 5.8 4.9 4.9 4.9 4.7 4.4 4.2 4.4 6.4 6.3 6.1 6.8 7.2 6.9 7.0 7.0 7.5
Std Deviation (1e-4) 0.9 1.1 1.1 1.2 1.7 1.5 1.5 1.5 1.3 1.1 1.3 1.1 1.1 1.0 1.1 1.7 2.0 2.0 1.4 1.4 1.7
Intensity Change (Log2) – – – 0.18 0.29 0.05 0.03 0.05 -0.03 -0.13 -0.19 -0.13 0.42 0.4 0.35 0.51 0.6 0.53 0.55 0.56 0.66


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3.5 5.9 10.8 13.4 14.5 15.9 15.9 16.9 17.7 5.8 5.7 10.2 5.2 4.9 5.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -1.0 0 -2.8 0 -1.8 0 0 0 0 3.3 2.5 1.0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -9.8 -8.0 -5.2 0 -10.8 0 0 0 0 -11.2 -13.4 -14.4 -8.3 -7.7 -8.0
Nucleus 0.4 -2.6 -4.3 -7.7 -8.8 -9.7 -10.2 -12.0 -11.6 -3.5 0.5 0 3.4 0 -0.5
Nuclear Periphery 0 0 2.9 0 1.5 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 7.2 7.2 3.7 2.4 3.3 1.5 1.4 -0.1 -1.0 4.8 5.4 4.1 0.8 2.9 2.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.9628 4.4618 3.9305 3.9867 3.0901 4.2424 0.7225 1.5709 1.6675 1.0691 1.4692 1.4652 0.9049 1.6339 1.416 1.525 1.8129 1.3937
Actin 0.0106 0.0001 0.0002 0.0008 0 0.0003 0.0316 0.0005 0.0024 0 0 0.0001 0.0107 0.0001 0.0013 0.0006 0.0108 0.0032
Bud 0.0005 0.0005 0.0047 0.0004 0.0006 0.0005 0.0027 0.0005 0.0008 0.0005 0.0002 0.0003 0.0063 0.005 0.0101 0.0029 0.0005 0.0014
Bud Neck 0.0024 0.0035 0.001 0.002 0.0019 0.0053 0.0169 0.0024 0.0014 0.0019 0.0006 0.0017 0.0016 0.0029 0.0025 0.0008 0.0021 0.0036
Bud Periphery 0.0013 0.0017 0.004 0.0011 0.0016 0.0017 0.0058 0.0013 0.0016 0.0011 0.0003 0.0008 0.0047 0.0028 0.0048 0.0075 0.0013 0.0021
Bud Site 0.0025 0.0031 0.0028 0.0013 0.0023 0.0014 0.0318 0.0021 0.006 0.0022 0.0006 0.0007 0.0126 0.0177 0.0058 0.0007 0.0013 0.0015
Cell Periphery 0.0034 0.0056 0.0037 0.003 0.0025 0.0041 0.0035 0.002 0.0019 0.0019 0.0009 0.0034 0.0017 0.0037 0.0024 0.0015 0.0022 0.0039
Cytoplasm 0.0109 0.0225 0.004 0.0022 0.0035 0.0068 0.0123 0.0121 0.0123 0.0039 0.0011 0.0164 0.0066 0.0066 0.0147 0.0035 0.0019 0.0064
Cytoplasmic Foci 0.021 0.0086 0.0237 0.0202 0.0076 0.0178 0.0378 0.01 0.0255 0.004 0.0045 0.0111 0.0241 0.0285 0.0198 0.0464 0.0135 0.0416
Eisosomes 0.0001 0 0 0 0 0.0001 0.0013 0 0.0001 0 0 0 0.0001 0 0 0 0.0002 0
Endoplasmic Reticulum 0.0038 0.0041 0.0051 0.0015 0.0017 0.0025 0.005 0.0039 0.007 0.0017 0.0008 0.0024 0.0038 0.0043 0.0033 0.0015 0.0019 0.0032
Endosome 0.2339 0.1267 0.1783 0.1283 0.1531 0.1452 0.2536 0.1604 0.2914 0.2001 0.2524 0.1421 0.2986 0.1782 0.2343 0.2124 0.2686 0.1901
Golgi 0.0134 0.0047 0.0073 0.0119 0.0033 0.005 0.0224 0.0056 0.0238 0.0063 0.0063 0.0052 0.0187 0.0044 0.0137 0.0066 0.0317 0.0066
Lipid Particles 0.0547 0.0194 0.0354 0.0244 0.0241 0.0295 0.0373 0.0125 0.0154 0.0049 0.0082 0.0268 0.0405 0.0085 0.0248 0.0304 0.0261 0.0177
Mitochondria 0.045 0.0152 0.0748 0.0157 0.0035 0.0144 0.0222 0.0151 0.0448 0.0021 0.0077 0.0055 0.0456 0.0243 0.1012 0.0139 0.0346 0.0147
None 0.0134 0.013 0.0049 0.0003 0.0028 0.0055 0.0459 0.0157 0.0125 0.0002 0.0001 0.0004 0.0015 0.0019 0.0124 0.0006 0.0003 0.0029
Nuclear Periphery 0.0056 0.0053 0.0072 0.0016 0.0021 0.0073 0.0122 0.0128 0.0292 0.0026 0.0022 0.0032 0.0151 0.0127 0.009 0.0026 0.0018 0.0074
Nucleolus 0.0089 0.0133 0.0071 0.004 0.012 0.0122 0.0076 0.0047 0.007 0.0045 0.0023 0.0045 0.0081 0.0127 0.0081 0.0184 0.0051 0.0111
Nucleus 0.018 0.0075 0.0061 0.0036 0.0056 0.0117 0.0095 0.0098 0.0176 0.0034 0.0015 0.0036 0.0091 0.0119 0.0108 0.0026 0.0028 0.0105
Peroxisomes 0.0062 0.0023 0.0073 0.0009 0.0034 0.0031 0.012 0.0006 0.0039 0.0005 0.0007 0.0013 0.0077 0.0014 0.0043 0.0026 0.0025 0.0029
Punctate Nuclear 0.0034 0.0004 0.0029 0.0001 0.0012 0.0019 0.0071 0.0005 0.0049 0.0001 0.0001 0.0002 0.0034 0.0044 0.0021 0.0028 0.0002 0.0041
Vacuole 0.4195 0.6271 0.4777 0.6546 0.6877 0.6011 0.3357 0.5864 0.3779 0.6554 0.5874 0.6471 0.3586 0.525 0.37 0.474 0.4868 0.5349
Vacuole Periphery 0.1214 0.1155 0.1418 0.1219 0.0794 0.1227 0.0857 0.1412 0.1128 0.1027 0.1222 0.1231 0.121 0.143 0.1445 0.1675 0.1039 0.1301

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 61.8692 53.4001 48.6824 62.5991 45.3636 47.7621 56.1139 65.3344 59.5122 60.7041
Translational Efficiency 1.2925 1.235 1.5284 0.9041 1.2462 1.7166 1.3041 0.9149 1.0993 0.995

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Putative metalloprotease with similarity to zinc carboxypeptidases; required for normal cell wall assembly
Localization
Cell Percentages vacuole (96%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Ecm14

Ecm14


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ecm14-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available