Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the large subunit
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.07 0.09 0.17 0.14 0.11 0.14 0.12 0.12 0.12 0.11 0.1 0.12 0.05 0.06 0 0 0.05 0.06 0.06 0.07 0.12 0.09 0.06 0.09
Bud 0.07 0.06 0.06 0.06 0.06 0.14 0.1 0.14 0.14 0.13 0.13 0.12 0.07 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.13 0.06 0.06 0.06 0.09
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.21 0.18 0.33 0.27 0.19 0.25 0.22 0.19 0.23 0.19 0.21 0.19 0.33 0.42 0.4 0.26 0.38 0.3 0.17 0.25 0.49 0.46 0.34 0.35
Cytoplasm 0.78 0.83 0.72 0.83 0.82 0.81 0.82 0.78 0.8 0.8 0.84 0.81 0.81 0.77 0.7 0.66 0.49 0.5 0.26 0.26 0.1 0.18 0.23 0.17
Endoplasmic Reticulum 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.15 0.07 0.11 0.07 0.09 0.1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0.07 0.07 0.1 0 0.05 0 0 0 0 0 0 0 0 0 0 0.06 0.08 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.17 0.15 0.2 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.08 0.11 0.16 0.2 0.14 0.05 0.05 0.07 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 13 18 38 22 28 54 46 61 42 45 36 57 6 11 9 4 10 9 12 16 28 17 18 31
Bud 13 11 14 9 16 54 38 72 49 53 51 58 8 2 6 3 1 2 5 4 1 2 5 9
Bud Neck 0 0 2 1 5 1 0 0 0 0 0 0 0 1 1 0 0 0 22 29 13 11 16 30
Bud Site 0 1 0 0 1 0 1 1 1 2 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 42 35 73 42 50 99 86 95 82 81 79 91 38 73 99 45 71 43 39 55 116 91 99 121
Cytoplasm 154 164 158 129 216 320 313 393 284 337 318 380 92 135 171 114 92 72 59 57 22 35 67 57
Endoplasmic Reticulum 9 3 2 1 5 10 4 11 6 12 9 5 1 4 11 2 3 0 34 15 26 13 26 34
Endosome 0 0 0 0 0 4 0 3 0 1 0 6 1 0 1 0 1 0 1 3 1 0 5 3
Golgi 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 3 2 1 2 1 0 0 0
Mitochondria 2 3 8 0 2 18 28 35 36 18 20 13 2 0 0 1 5 2 3 4 6 12 22 18
Nucleus 1 1 0 1 2 3 0 5 1 4 3 2 2 1 0 29 28 29 0 0 0 0 1 0
Nuclear Periphery 0 0 0 0 0 0 2 2 0 1 0 2 1 1 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 2 1 3 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 3 1 1 2 1 1 1 0 1 2 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 2 0 2 7 1 15 4 1 5 1 3 0 0 3 0 0 1 0 0 1 0 0 0
Vac/Vac Membrane 1 1 1 0 5 2 6 5 2 18 9 22 0 0 0 13 20 23 45 30 12 9 20 26
Unique Cell Count 198 198 219 156 263 397 383 503 353 419 378 470 114 175 246 173 186 145 228 222 236 199 291 342
Labelled Cell Count 237 239 300 208 338 568 541 690 506 580 527 642 151 228 302 212 234 183 228 222 236 199 291 342


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.1 5.7 5.7 6.1 6.3 5.7 6.3 6.6 6.1 6.4 6.6 6.4 6.2 6.4 6.6 11.2 11.3 11.0 5.7 5.7 6.3
Std Deviation (1e-4) 1.7 1.4 1.7 2.2 2.4 1.7 3.2 1.6 1.5 1.4 1.6 1.3 1.4 1.3 1.2 2.7 3.2 2.6 1.3 1.3 1.7
Intensity Change (Log2) – – – 0.09 0.14 -0.01 0.13 0.2 0.1 0.17 0.21 0.16 0.1 0.17 0.2 0.96 0.98 0.94 -0.02 -0.01 0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.8 -2.1 -1.2 -1.8 -1.9 -1.8 -2.4 -2.8 -1.9 -3.1 -3.3 -4.9 -4.8 -3.7 -3.1
Bud -0.2 -0.1 2.7 1.5 3.0 2.8 2.4 2.7 2.4 0.2 -2.6 -2.1 -2.2 -3.1 -2.3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.3 -3.6 -2.2 -2.9 -4.2 -2.6 -3.9 -3.4 -4.0 0 1.7 1.5 -1.6 1.0 -0.7
Cytoplasm 2.4 2.6 2.4 2.7 1.7 2.3 2.4 3.5 2.6 1.7 1.1 -0.6 -1.3 -4.7 -4.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 2.3 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.5 1.8 1.7 2.9 0.4 0.9 -0.6 0 0 0 0 -0.5 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 6.3 6.0 6.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 3.0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 2.7 0 2.9 0 0 0 3.7 4.7 5.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.1283 0.6311 0.628 -0.0791 -0.3614 0.5181 1.3273 3.0695 2.5059 1.8781 2.0115 2.2926 0.3253 0.9019 1.2383 1.0665 0.4417 1.1291
Actin 0.0246 0.0147 0.0135 0.0109 0.008 0.0107 0.0116 0.0022 0.0125 0.0066 0.0207 0.0052 0.0201 0.0031 0.0124 0.0019 0.0177 0.0033
Bud 0.0006 0.0009 0.001 0.0022 0.0019 0.0018 0.0028 0.0006 0.0017 0.0009 0.0018 0.0012 0.0002 0.0004 0.001 0.0006 0.0003 0.0006
Bud Neck 0.0095 0.0056 0.0166 0.0309 0.006 0.0376 0.0032 0.0084 0.0183 0.0353 0.0157 0.0495 0.014 0.0041 0.012 0.01 0.0022 0.0221
Bud Periphery 0.0043 0.0032 0.0071 0.0203 0.0128 0.0138 0.0124 0.0026 0.0131 0.0045 0.0139 0.0079 0.002 0.0017 0.0047 0.0058 0.0012 0.0062
Bud Site 0.0166 0.0983 0.0666 0.0113 0.0052 0.0038 0.0152 0.1026 0.0417 0.0176 0.0035 0.002 0.0153 0.1009 0.0397 0.0019 0.0007 0.0031
Cell Periphery 0.16 0.1426 0.1798 0.2075 0.0797 0.1181 0.1475 0.1047 0.1874 0.1458 0.1774 0.1301 0.1752 0.1091 0.2244 0.2786 0.1145 0.2488
Cytoplasm 0.4141 0.512 0.4228 0.3371 0.5923 0.5591 0.5146 0.5903 0.5713 0.5536 0.4901 0.6223 0.3262 0.3144 0.4118 0.5259 0.4419 0.5636
Cytoplasmic Foci 0.0137 0.0064 0.004 0.012 0.0037 0.0049 0.0097 0.0029 0.0041 0.0045 0.0029 0.0095 0.027 0.0068 0.0062 0.0035 0.0113 0.0119
Eisosomes 0.0045 0.0038 0.0029 0.004 0.001 0.0014 0.0015 0.0005 0.0013 0.0004 0.0009 0.0003 0.0043 0.0019 0.0022 0.0009 0.0002 0.0003
Endoplasmic Reticulum 0.0071 0.0034 0.0042 0.0038 0.0052 0.0038 0.0071 0.004 0.0051 0.0066 0.0077 0.0032 0.0048 0.0026 0.006 0.0025 0.0016 0.0031
Endosome 0.0072 0.0013 0.0006 0.0048 0.0009 0.0011 0.0033 0.0008 0.002 0.0011 0.0014 0.0047 0.0094 0.0004 0.0065 0.0005 0.0229 0.0068
Golgi 0.0023 0.0011 0.0007 0.001 0.0006 0.0007 0.0016 0.0003 0.0014 0.0008 0.0024 0.0021 0.0025 0.0004 0.005 0.0003 0.0838 0.0013
Lipid Particles 0.0084 0.001 0.0012 0.0029 0.0013 0.0016 0.0066 0.0027 0.002 0.0058 0.0041 0.0026 0.0132 0.002 0.0023 0.0014 0.0021 0.0088
Mitochondria 0.0012 0.0003 0.0003 0.001 0.0002 0.0005 0.001 0.0001 0.0007 0.0002 0.0048 0.0006 0.0004 0.0002 0.0136 0.0005 0.0029 0.0007
None 0.3068 0.2015 0.2731 0.3232 0.2758 0.2339 0.2469 0.1607 0.1265 0.1935 0.2441 0.1451 0.3549 0.4448 0.2422 0.1581 0.2906 0.094
Nuclear Periphery 0.0013 0.0003 0.0003 0.0005 0.0003 0.0006 0.0013 0.0003 0.0005 0.0004 0.0004 0.0004 0.0018 0.0003 0.0005 0.0001 0.0001 0.0084
Nucleolus 0.0017 0.0003 0.0005 0.0007 0.0002 0.0003 0.0005 0.0002 0.0003 0.0003 0.0002 0.0003 0.0002 0.0005 0.0003 0.0004 0 0.0004
Nucleus 0.0011 0.0007 0.0007 0.0009 0.0007 0.0009 0.001 0.0007 0.0013 0.0008 0.0004 0.001 0.0037 0.0005 0.0006 0.0004 0.0001 0.001
Peroxisomes 0.0022 0.0001 0.0002 0.0013 0.0001 0.0002 0.0016 0 0.0007 0.0001 0.0008 0.0004 0.014 0.0004 0.0004 0.0002 0.0014 0.002
Punctate Nuclear 0.0033 0.0005 0.0005 0.0019 0.0002 0.0005 0.0021 0.0002 0.0003 0.0005 0.0002 0.001 0.0056 0.0005 0.0003 0.0001 0.0001 0.0063
Vacuole 0.0094 0.0021 0.0034 0.0214 0.0037 0.0047 0.0083 0.0151 0.0077 0.0207 0.0067 0.0105 0.0051 0.0051 0.0073 0.0061 0.004 0.007
Vacuole Periphery 0.0003 0.0001 0.0001 0.0003 0.0001 0.0001 0.0002 0 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0006 0.0001 0.0003 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 31.648 15.2927 26.1 26.8192 21.071 25.6464 24.3533 34.9608 32.4129 33.2875
Translational Efficiency 1.1255 1.96 1.197 1.247 1.6113 1.7472 1.9666 1.1436 1.6303 1.4332

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the large subunit
Localization
Cell Percentages cell periphery (67%)
Cell Cycle Regulation No
Subcompartmental Group cell periphery-3

Mrpl6

Mrpl6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrpl6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available