Standard name | |
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Human Ortholog | |
Description | Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1801 has a paralog, YAP1802, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 94 | 162 | 82 | 62 | 93 | 131 | 132 | – | 54 | 100 | 80 | 95 | 60 | 117 | 91 | 123 | 114 | 97 | 98 | 174 | 97 | 32 | 73 | 100 |
Bud | 22 | 48 | 23 | 23 | 30 | 45 | 42 | – | 21 | 44 | 30 | 30 | 18 | 20 | 26 | 38 | 22 | 28 | 12 | 17 | 17 | 35 | 76 | 49 |
Bud Neck | 0 | 0 | 2 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 27 | 34 | 33 | 57 | 87 | 54 |
Bud Site | 35 | 31 | 10 | 14 | 14 | 24 | 30 | – | 9 | 11 | 9 | 16 | 15 | 3 | 1 | 13 | 22 | 11 | – | – | – | – | – | – |
Cell Periphery | 15 | 41 | 20 | 27 | 16 | 20 | 44 | – | 20 | 51 | 18 | 39 | 29 | 81 | 128 | 58 | 50 | 42 | 12 | 21 | 14 | 2 | 4 | 12 |
Cytoplasm | 10 | 43 | 31 | 40 | 59 | 40 | 79 | – | 45 | 46 | 38 | 38 | 34 | 74 | 94 | 75 | 54 | 31 | 0 | 1 | 2 | 10 | 28 | 26 |
Endoplasmic Reticulum | 9 | 23 | 8 | 6 | 17 | 43 | 33 | – | 29 | 35 | 35 | 35 | 6 | 28 | 26 | 9 | 16 | 11 | 3 | 6 | 3 | 0 | 2 | 5 |
Endosome | 2 | 2 | 1 | 0 | 0 | 1 | 4 | – | 7 | 4 | 2 | 2 | 0 | 1 | 0 | 4 | 4 | 4 | 2 | 3 | 5 | 3 | 2 | 6 |
Golgi | 1 | 1 | 0 | 0 | 0 | 0 | 4 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 11 | 8 | 5 | 2 | 1 | 0 | 0 | 0 | 0 |
Mitochondria | 19 | 3 | 0 | 1 | 0 | 19 | 19 | – | 70 | 50 | 120 | 79 | 0 | 1 | 0 | 9 | 4 | 8 | 8 | 3 | 5 | 3 | 8 | 4 |
Nucleus | 1 | 5 | 2 | 5 | 7 | 5 | 9 | – | 1 | 7 | 0 | 3 | 5 | 2 | 2 | 9 | 4 | 2 | 0 | 1 | 0 | 0 | 2 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 4 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | 1 | – | 2 | 4 | 1 | 0 | 0 | 0 | 0 | 3 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 12 | 9 | 0 | 3 | 6 | 9 | 16 | – | 6 | 13 | 5 | 12 | 1 | 3 | 0 | 5 | 14 | 3 | 1 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 2 | 2 | 5 | 9 | 14 | 2 | 22 | – | 1 | 7 | 2 | 2 | 5 | 14 | 3 | 29 | 31 | 19 | 0 | 0 | 0 | 0 | 2 | 0 |
Vac/Vac Membrane | 0 | 1 | 3 | 0 | 8 | 4 | 12 | – | 5 | 13 | 2 | 5 | 0 | 1 | 0 | 9 | 13 | 7 | 1 | 3 | 2 | 1 | 5 | 6 |
Unique Cell Count | 125 | 234 | 138 | 134 | 193 | 226 | 297 | 190 | 246 | 211 | 228 | 118 | 243 | 256 | 273 | 247 | 189 | 172 | 269 | 188 | 151 | 302 | 279 | |
Labelled Cell Count | 222 | 371 | 187 | 190 | 265 | 343 | 447 | 271 | 390 | 342 | 358 | 173 | 345 | 372 | 395 | 361 | 270 | 172 | 269 | 188 | 151 | 302 | 279 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.3 | 8.9 | 8.8 | 8.4 | 9.5 | 7.8 | 8.0 | – | 6.6 | 7.6 | 6.5 | 7.2 | 8.3 | 8.6 | 8.8 | 11.5 | 11.7 | 11.4 | 7.3 | 7.4 | 7.5 |
Std Deviation (1e-4) | 1.1 | 1.4 | 1.5 | 1.8 | 2.1 | 1.8 | 1.7 | – | 1.3 | 1.7 | 1.5 | 1.7 | 1.1 | 1.2 | 1.4 | 2.0 | 2.0 | 2.1 | 1.0 | 1.3 | 1.0 |
Intensity Change (Log2) | – | – | – | -0.07 | 0.1 | -0.18 | -0.14 | – | -0.42 | -0.22 | -0.45 | -0.3 | -0.08 | -0.03 | -0.01 | 0.38 | 0.41 | 0.37 | -0.28 | -0.25 | -0.23 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -2.2 | -2.0 | -0.3 | -2.9 | – | -5.6 | -3.5 | -3.9 | -3.3 | -1.4 | -2.1 | -4.6 | -2.8 | -2.5 | -1.5 |
Bud | 0.1 | -0.3 | 0.8 | -0.7 | – | -1.5 | 0.3 | -0.6 | -0.9 | -0.3 | -2.5 | -1.9 | -0.7 | -2.3 | -0.5 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0.9 | 0 | 1.1 | 1.0 | – | -1.0 | -1.1 | -1.2 | -0.1 | 1.5 | 0 | 0 | -1.0 | 0.6 | -0.5 |
Cell Periphery | 1.2 | -1.8 | -1.7 | 0.1 | – | -1.1 | 1.5 | -1.7 | 0.7 | 2.0 | 4.0 | 6.9 | 1.6 | 1.4 | 1.8 |
Cytoplasm | 1.4 | 1.6 | -1.1 | 0.9 | – | 0.3 | -0.9 | -1.0 | -1.4 | 1.2 | 1.7 | 2.9 | 1.1 | -0.1 | -1.4 |
Endoplasmic Reticulum | -0.5 | 1.0 | 3.5 | 1.8 | – | 2.7 | 2.5 | 3.0 | 2.8 | -0.2 | 1.8 | 1.5 | -1.2 | 0.3 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 3.5 | 3.0 | – | 8.0 | 5.7 | 10.9 | 7.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 2.8 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.8 | 0 |
SpindlePole | 1.2 | 1.4 | 0 | 1.5 | – | 0 | 0 | 0 | 0 | 0 | 0.9 | 0 | 2.4 | 2.9 | 2.2 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 1.5 | 0 | 0 | 0 | 0 | 0 | 0 | 1.5 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.4048 | 2.9644 | 2.9693 | 2.3291 | 1.9253 | 3.074 | 4.4116 | 4.0742 | 4.0968 | 3.866 | 3.8336 | 4.3478 | 2.375 | 3.4343 | 2.4836 | 2.3375 | 1.5794 | 2.8016 |
Actin | 0.4138 | 0.2431 | 0.6055 | 0.5195 | 0.5815 | 0.3846 | 0.368 | 0.2906 | 0.5504 | 0.6354 | 0.6172 | 0.3641 | 0.5089 | 0.2054 | 0.6009 | 0.5382 | 0.6172 | 0.4166 |
Bud | 0.0044 | 0.0023 | 0.0084 | 0.0074 | 0.0091 | 0.0034 | 0.0054 | 0.0064 | 0.0187 | 0.0107 | 0.0075 | 0.0029 | 0.0023 | 0.0043 | 0.0133 | 0.0181 | 0.0081 | 0.0048 |
Bud Neck | 0.1042 | 0.0248 | 0.0952 | 0.218 | 0.0708 | 0.4357 | 0.089 | 0.0183 | 0.1079 | 0.062 | 0.0247 | 0.5529 | 0.0713 | 0.0223 | 0.0958 | 0.0809 | 0.0597 | 0.4779 |
Bud Periphery | 0.0116 | 0.0039 | 0.0466 | 0.0733 | 0.0777 | 0.029 | 0.0237 | 0.008 | 0.093 | 0.0778 | 0.0357 | 0.0195 | 0.0068 | 0.0055 | 0.0524 | 0.1285 | 0.0344 | 0.0153 |
Bud Site | 0.2646 | 0.6478 | 0.1315 | 0.0391 | 0.0111 | 0.0091 | 0.2431 | 0.6177 | 0.1224 | 0.0171 | 0.0202 | 0.0069 | 0.1719 | 0.5836 | 0.1182 | 0.0435 | 0.0272 | 0.0057 |
Cell Periphery | 0.0395 | 0.0258 | 0.0301 | 0.0445 | 0.0468 | 0.0443 | 0.0783 | 0.0089 | 0.0228 | 0.0394 | 0.0222 | 0.0173 | 0.0531 | 0.0162 | 0.0427 | 0.0345 | 0.0495 | 0.0282 |
Cytoplasm | 0.0202 | 0.0462 | 0.0153 | 0.0106 | 0.0399 | 0.0373 | 0.0555 | 0.0407 | 0.0236 | 0.0439 | 0.03 | 0.0101 | 0.0559 | 0.1226 | 0.0513 | 0.0135 | 0.0129 | 0.0125 |
Cytoplasmic Foci | 0.0168 | 0.0009 | 0.0098 | 0.0013 | 0.004 | 0.0078 | 0.0104 | 0.0027 | 0.007 | 0.0242 | 0.0035 | 0.0035 | 0.0162 | 0.0153 | 0.0038 | 0.0177 | 0.0074 | 0.0025 |
Eisosomes | 0.0269 | 0.0034 | 0.0062 | 0.0191 | 0.0568 | 0.0057 | 0.0229 | 0.0035 | 0.0118 | 0.0182 | 0.0583 | 0.0094 | 0.0168 | 0.0017 | 0.0068 | 0.0085 | 0.0154 | 0.0087 |
Endoplasmic Reticulum | 0.0022 | 0.0003 | 0.0032 | 0.0015 | 0.0018 | 0.0013 | 0.0057 | 0.0002 | 0.0012 | 0.0047 | 0.0214 | 0.0015 | 0.0032 | 0.0024 | 0.0008 | 0.0036 | 0.0014 | 0.0007 |
Endosome | 0.0205 | 0.0001 | 0.0044 | 0.0006 | 0.0029 | 0.006 | 0.0104 | 0.0001 | 0.0061 | 0.0193 | 0.0275 | 0.003 | 0.016 | 0.002 | 0.0028 | 0.0193 | 0.004 | 0.0051 |
Golgi | 0.0174 | 0.0004 | 0.0092 | 0.0037 | 0.0066 | 0.016 | 0.0044 | 0.0016 | 0.0028 | 0.0108 | 0.0152 | 0.0032 | 0.0073 | 0.0032 | 0.005 | 0.0058 | 0.0067 | 0.0055 |
Lipid Particles | 0.0116 | 0.0001 | 0.0036 | 0.0009 | 0.0045 | 0.0061 | 0.0044 | 0.0002 | 0.0012 | 0.0055 | 0.0051 | 0.0008 | 0.014 | 0.0022 | 0.001 | 0.0224 | 0.0027 | 0.0042 |
Mitochondria | 0.0173 | 0 | 0.007 | 0.0583 | 0.073 | 0.0042 | 0.0036 | 0.0001 | 0.0008 | 0.0033 | 0.0063 | 0.0006 | 0.0043 | 0.0002 | 0.0009 | 0.0009 | 0.0025 | 0.0015 |
None | 0.0062 | 0.0002 | 0.0072 | 0.0004 | 0.0004 | 0.0021 | 0.0414 | 0.0002 | 0.0134 | 0.0073 | 0.0099 | 0.0009 | 0.0237 | 0.0089 | 0.0008 | 0.0435 | 0.1428 | 0.0005 |
Nuclear Periphery | 0.0024 | 0.0001 | 0.0011 | 0.0002 | 0.0002 | 0.0001 | 0.0093 | 0.0001 | 0.0038 | 0.0072 | 0.0292 | 0.0007 | 0.0037 | 0.0005 | 0.0002 | 0.0046 | 0.0011 | 0.0031 |
Nucleolus | 0.0008 | 0 | 0.004 | 0.0001 | 0.0001 | 0.0001 | 0.0005 | 0 | 0.0003 | 0.0003 | 0.0022 | 0.0001 | 0.0007 | 0.0001 | 0.0001 | 0.0007 | 0.0005 | 0.0003 |
Nucleus | 0.002 | 0.0001 | 0.001 | 0.0002 | 0.0002 | 0.0002 | 0.0096 | 0.0001 | 0.0045 | 0.002 | 0.052 | 0.0003 | 0.0009 | 0.0005 | 0.0002 | 0.0011 | 0.0006 | 0.0016 |
Peroxisomes | 0.0087 | 0.0002 | 0.0063 | 0.0003 | 0.0118 | 0.0061 | 0.0073 | 0.0004 | 0.0039 | 0.0052 | 0.0007 | 0.0011 | 0.0177 | 0.0012 | 0.0021 | 0.0038 | 0.0027 | 0.0014 |
Punctate Nuclear | 0.0051 | 0.0001 | 0.001 | 0.0001 | 0.0001 | 0.0003 | 0.0049 | 0.0002 | 0.0034 | 0.0013 | 0.0034 | 0.0004 | 0.003 | 0.0017 | 0.0003 | 0.009 | 0.0019 | 0.0028 |
Vacuole | 0.0029 | 0.0001 | 0.0029 | 0.0006 | 0.0004 | 0.0005 | 0.0016 | 0.0001 | 0.0008 | 0.0031 | 0.005 | 0.0006 | 0.0015 | 0.0004 | 0.0004 | 0.0014 | 0.001 | 0.0008 |
Vacuole Periphery | 0.001 | 0 | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0006 | 0 | 0.0002 | 0.0014 | 0.0029 | 0.0001 | 0.001 | 0 | 0.0001 | 0.0006 | 0.0003 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.5475 | 13.9144 | 12.2371 | 22.9959 | 10.4642 | 27.0801 | 23.5783 | 32.1792 | 26.5269 | 23.9983 |
Translational Efficiency | 1.1752 | 0.7985 | 0.8898 | 0.5621 | 1.1678 | 0.9181 | 0.7882 | 0.6183 | 0.8756 | 0.71 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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