Standard name | |
---|---|
Human Ortholog | |
Description | One of two mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for respiratory growth on rich medium, but required for respiratory growth on minimal medium; see also PTH2 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 3 | 0 | 2 | 2 | 0 | 0 | – | 0 | 2 | 1 | 0 | 3 | 0 | 0 | 2 | 7 | 5 | 0 | 0 | 2 | 3 | 13 | 16 |
Bud | 0 | 1 | 0 | 4 | 4 | 7 | 2 | – | 21 | 16 | 16 | 28 | 3 | 1 | 3 | 8 | 5 | 6 | 0 | 0 | 0 | 1 | 8 | 11 |
Bud Neck | 0 | 0 | 2 | 8 | 11 | 7 | 18 | – | 3 | 14 | 4 | 4 | 3 | 0 | 0 | 2 | 1 | 3 | 1 | 0 | 1 | 0 | 5 | 14 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 1 | – | 4 | 2 | 9 | 10 | 0 | 0 | 0 | 0 | 1 | 2 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 5 | 0 | 0 | 4 | – | 2 | 4 | 0 | 3 | 1 | 3 | 2 | 5 | 3 | 6 | 0 | 0 | 0 | 0 | 2 | 5 |
Cytoplasm | 10 | 57 | 25 | 239 | 224 | 111 | 211 | – | 159 | 114 | 78 | 124 | 217 | 375 | 459 | 61 | 37 | 41 | 10 | 54 | 18 | 42 | 117 | 107 |
Endoplasmic Reticulum | 1 | 2 | 1 | 0 | 0 | 3 | 2 | – | 2 | 1 | 4 | 0 | 2 | 0 | 0 | 11 | 9 | 5 | 0 | 0 | 0 | 1 | 3 | 11 |
Endosome | 0 | 0 | 0 | 1 | 8 | 0 | 14 | – | 3 | 5 | 7 | 7 | 5 | 24 | 26 | 32 | 12 | 19 | 2 | 2 | 3 | 10 | 26 | 26 |
Golgi | 0 | 1 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 1 | 1 | 3 |
Mitochondria | 12 | 11 | 0 | 11 | 18 | 100 | 105 | – | 292 | 296 | 298 | 368 | 9 | 5 | 15 | 16 | 16 | 14 | 35 | 37 | 43 | 88 | 166 | 212 |
Nucleus | 0 | 0 | 0 | 1 | 1 | 7 | 12 | – | 14 | 10 | 4 | 6 | 0 | 1 | 0 | 2 | 4 | 3 | 0 | 0 | 0 | 0 | 1 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 1 | 2 | 0 | 1 | – | 0 | 4 | 2 | 5 | 2 | 6 | 18 | 9 | 3 | 9 | 0 | 0 | 0 | 1 | 3 | 5 |
SpindlePole | 0 | 1 | 0 | 15 | 29 | 12 | 56 | – | 29 | 50 | 26 | 50 | 5 | 8 | 7 | 25 | 18 | 8 | 3 | 2 | 4 | 9 | 21 | 23 |
Vac/Vac Membrane | 0 | 0 | 0 | 3 | 13 | 2 | 28 | – | 12 | 10 | 7 | 15 | 4 | 6 | 19 | 21 | 15 | 26 | 4 | 5 | 3 | 3 | 10 | 13 |
Unique Cell Count | 20 | 71 | 27 | 274 | 290 | 197 | 412 | 444 | 441 | 366 | 485 | 243 | 418 | 517 | 156 | 107 | 117 | 61 | 112 | 82 | 171 | 401 | 469 | |
Labelled Cell Count | 24 | 76 | 28 | 291 | 313 | 250 | 454 | 543 | 528 | 458 | 622 | 255 | 430 | 549 | 196 | 134 | 147 | 61 | 112 | 82 | 171 | 401 | 469 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.3 | 3.8 | 4.7 | 4.4 | 5.0 | 3.4 | 4.1 | – | 3.7 | 3.8 | 3.5 | 3.7 | 4.7 | 4.7 | 4.8 | 5.6 | 6.0 | 6.0 | 4.3 | 4.3 | 4.5 |
Std Deviation (1e-4) | 0.3 | 0.6 | 0.5 | 1.1 | 1.3 | 1.1 | 0.9 | – | 1.5 | 1.1 | 0.6 | 0.6 | 1.5 | 1.3 | 1.4 | 1.3 | 1.1 | 1.3 | 1.9 | 1.4 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.08 | 0.1 | -0.47 | -0.19 | – | -0.34 | -0.31 | -0.41 | -0.34 | 0.01 | 0.02 | 0.04 | 0.26 | 0.36 | 0.35 | -0.12 | -0.12 | -0.05 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.7356 | -0.7229 | -0.8459 | -0.5024 | -0.0015 | -0.7421 | -1.3239 | -0.2521 | -0.7068 | -0.7489 | -1.3588 | -0.5406 | 1.0243 | 0.5247 | 0.2975 | 0.2495 | 0.2238 | 0.4896 |
Actin | 0.0169 | 0.0039 | 0.0048 | 0.01 | 0.0353 | 0.0081 | 0.0166 | 0.0012 | 0.0026 | 0.0039 | 0.0014 | 0.0023 | 0.045 | 0.0038 | 0.0077 | 0.0002 | 0.0002 | 0.0008 |
Bud | 0.0081 | 0.0138 | 0.0184 | 0.0077 | 0.0847 | 0.0145 | 0.0008 | 0.0008 | 0.0021 | 0.001 | 0.0011 | 0.0003 | 0.0004 | 0.0006 | 0.0005 | 0.0002 | 0.0001 | 0.0006 |
Bud Neck | 0.0078 | 0.0008 | 0.0044 | 0.0058 | 0.0032 | 0.0042 | 0.0024 | 0.0013 | 0.0046 | 0.0022 | 0.0003 | 0.0193 | 0.0028 | 0.0002 | 0.0006 | 0.0004 | 0.0002 | 0.0143 |
Bud Periphery | 0.0052 | 0.0062 | 0.0083 | 0.0061 | 0.0272 | 0.0081 | 0.0022 | 0.0014 | 0.0027 | 0.0038 | 0.0026 | 0.0008 | 0.0007 | 0.0008 | 0.0009 | 0.0005 | 0.0001 | 0.0019 |
Bud Site | 0.0179 | 0.0404 | 0.0251 | 0.0388 | 0.0181 | 0.0014 | 0.0061 | 0.0094 | 0.0087 | 0.0008 | 0.0005 | 0.0003 | 0.0047 | 0.0038 | 0.0021 | 0.0002 | 0.0001 | 0.0013 |
Cell Periphery | 0.0021 | 0.0017 | 0.0022 | 0.0008 | 0.0022 | 0.0011 | 0.0009 | 0.0014 | 0.0008 | 0.0009 | 0.0002 | 0.0004 | 0.0007 | 0.0005 | 0.0008 | 0.0006 | 0.0001 | 0.0011 |
Cytoplasm | 0.0919 | 0.1118 | 0.0874 | 0.0636 | 0.0809 | 0.1409 | 0.1327 | 0.2361 | 0.1693 | 0.2774 | 0.0969 | 0.2828 | 0.0965 | 0.1474 | 0.1465 | 0.2332 | 0.1898 | 0.2496 |
Cytoplasmic Foci | 0.086 | 0.1172 | 0.1048 | 0.0798 | 0.0882 | 0.1187 | 0.1142 | 0.0924 | 0.1083 | 0.0632 | 0.0581 | 0.08 | 0.1039 | 0.0511 | 0.0539 | 0.0231 | 0.0421 | 0.0742 |
Eisosomes | 0.0012 | 0.0007 | 0.0025 | 0.0006 | 0.0007 | 0.0004 | 0.0009 | 0.0008 | 0.0009 | 0.0006 | 0.0004 | 0.0002 | 0.0012 | 0.0002 | 0.0006 | 0.0001 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0085 | 0.0049 | 0.0026 | 0.0011 | 0.005 | 0.0027 | 0.0071 | 0.0051 | 0.0081 | 0.0038 | 0.0038 | 0.0035 | 0.006 | 0.0027 | 0.0038 | 0.0042 | 0.0012 | 0.0035 |
Endosome | 0.069 | 0.0468 | 0.0329 | 0.0322 | 0.0294 | 0.0365 | 0.0959 | 0.0485 | 0.0729 | 0.0221 | 0.0801 | 0.0636 | 0.0366 | 0.0066 | 0.0273 | 0.0147 | 0.0294 | 0.0313 |
Golgi | 0.0082 | 0.0064 | 0.0065 | 0.0082 | 0.0098 | 0.0066 | 0.009 | 0.0076 | 0.0078 | 0.0029 | 0.0112 | 0.0068 | 0.0114 | 0.0033 | 0.0049 | 0.0005 | 0.0041 | 0.0031 |
Lipid Particles | 0.0181 | 0.0097 | 0.0135 | 0.0097 | 0.03 | 0.008 | 0.0245 | 0.0099 | 0.0068 | 0.0016 | 0.012 | 0.0059 | 0.033 | 0.0017 | 0.0068 | 0.0013 | 0.0694 | 0.0044 |
Mitochondria | 0.018 | 0.0175 | 0.025 | 0.0115 | 0.0367 | 0.0311 | 0.0134 | 0.0266 | 0.0363 | 0.0494 | 0.0775 | 0.0276 | 0.0078 | 0.0017 | 0.008 | 0.0026 | 0.0367 | 0.01 |
None | 0.5664 | 0.5883 | 0.6104 | 0.6834 | 0.437 | 0.5835 | 0.5416 | 0.5294 | 0.5451 | 0.5412 | 0.633 | 0.4897 | 0.5765 | 0.7665 | 0.7121 | 0.6767 | 0.6053 | 0.5928 |
Nuclear Periphery | 0.0185 | 0.0019 | 0.0013 | 0.0012 | 0.0153 | 0.0022 | 0.0028 | 0.001 | 0.002 | 0.0013 | 0.0015 | 0.0007 | 0.0082 | 0.0004 | 0.0008 | 0.0007 | 0.0006 | 0.0009 |
Nucleolus | 0.0063 | 0.0019 | 0.0039 | 0.003 | 0.0078 | 0.0016 | 0.0008 | 0.0023 | 0.0013 | 0.0006 | 0.0006 | 0.0005 | 0.0021 | 0.0002 | 0.0003 | 0.0007 | 0.0003 | 0.0003 |
Nucleus | 0.0123 | 0.002 | 0.0052 | 0.0028 | 0.0143 | 0.003 | 0.0015 | 0.002 | 0.0023 | 0.0016 | 0.0012 | 0.0011 | 0.0038 | 0.0006 | 0.0006 | 0.0022 | 0.0009 | 0.0007 |
Peroxisomes | 0.0095 | 0.0107 | 0.0275 | 0.021 | 0.0119 | 0.006 | 0.0078 | 0.0075 | 0.0055 | 0.0124 | 0.0056 | 0.0026 | 0.0237 | 0.0056 | 0.015 | 0.0005 | 0.0119 | 0.0035 |
Punctate Nuclear | 0.0158 | 0.0041 | 0.0061 | 0.0083 | 0.0541 | 0.0146 | 0.0062 | 0.0013 | 0.0022 | 0.0009 | 0.0012 | 0.0018 | 0.0287 | 0.0006 | 0.0013 | 0.0009 | 0.0006 | 0.0018 |
Vacuole | 0.0097 | 0.0077 | 0.0058 | 0.0036 | 0.0059 | 0.0052 | 0.0098 | 0.0125 | 0.008 | 0.0062 | 0.0079 | 0.0084 | 0.005 | 0.0013 | 0.0047 | 0.0353 | 0.0054 | 0.003 |
Vacuole Periphery | 0.0028 | 0.0016 | 0.0014 | 0.0009 | 0.0023 | 0.0015 | 0.003 | 0.0015 | 0.0018 | 0.0022 | 0.0028 | 0.0014 | 0.0011 | 0.0002 | 0.0009 | 0.0013 | 0.0013 | 0.0008 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 5.777 | 5.1643 | 1.2774 | 5.9686 | 4.9934 | 5.7738 | 9.5729 | 14.0969 | 10.7311 | 8.9069 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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