Standard name
Human Ortholog
Description F-box protein containing five copies of the WD40 motif; controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus; dissociation of Met30p from SCF complex in response to cadmium stress is regulated by Cdc48p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0
Bud 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.68 0.75 0.62 0.95 0.82 0.85 0.54 0.62 0.55 0.65 0.85 0.95 0.91 0.94 0.94 0.47 0.59 0.33 0.68 0.74 0.73
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0 0.05 0 0 0 0 0 0
Endosome 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.23 0 0.12 0 0.24 0.11 0.54 0.41 0.62 0 0 0 0.09 0 0 0.08 0 0.14 0 0 0
Nucleus 0.08 0 0 0 0 0 0.09 0.06 0.05 0.27 0 0 0 0 0 0.15 0.11 0.08 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0
Vac/Vac Membrane 0.15 0.24 0.12 0 0 0 0 0 0 0.12 0.12 0 0.06 0.08 0.06 0.18 0.16 0.2 0.07 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 2 4 3 5 5
Bud 0 0 1 0 0 0 1 0 1 0 0 0 0 0 0 1 2 0 0 2 3
Bud Neck 1 0 0 0 0 3 0 3 0 1 0 1 0 0 0 1 2 0 2 1 4
Bud Site 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0
Cell Periphery 0 0 1 0 0 0 0 0 0 1 1 1 2 2 3 0 0 0 0 0 0
Cytoplasm 103 190 10 60 31 76 37 48 33 68 137 193 106 78 94 85 170 22 106 160 183
Endoplasmic Reticulum 1 3 0 0 0 0 1 0 0 0 0 2 15 3 5 0 0 0 0 0 0
Endosome 0 7 1 0 0 1 0 0 0 2 6 2 2 1 2 0 4 2 3 2 4
Golgi 0 0 1 1 0 0 0 0 0 0 0 1 1 2 0 0 0 0 0 0 0
Mitochondria 34 4 2 0 9 10 37 32 37 1 3 1 10 2 3 13 10 9 4 8 9
Nucleus 12 8 0 2 1 4 6 5 3 28 3 3 0 1 1 26 31 5 6 6 7
Nuclear Periphery 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 0 0 0 0 0 1 2 0 0 0 0 0 0 1 0
SpindlePole 0 0 0 0 0 0 1 2 1 0 2 0 1 0 1 5 6 3 8 8 10
Vac/Vac Membrane 22 61 2 1 0 2 1 3 2 13 20 5 7 7 6 31 44 13 11 6 12
Unique Cell Count 151 253 16 63 38 89 68 78 60 105 161 204 117 83 100 181 287 67 157 216 252
Labelled Cell Count 174 273 18 64 42 96 87 93 78 114 173 210 146 96 115 181 287 67 157 216 252


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 4.4 5.9 6.1 4.8 5.2 3.8 3.7 4.2 5.5 5.9 5.5 6.7 6.6 7.2 4.8 5.2 5.4
Std Deviation (1e-4) 0.8 1.0 2.0 2.7 1.8 2.3 1.3 1.0 2.3 1.1 1.9 1.6 3.3 3.2 4.7 0.7 1.4 1.3
Intensity Change (Log2)


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin
Bud
Bud Neck
Bud Periphery
Bud Site
Cell Periphery
Cytoplasm
Cytoplasmic Foci
Eisosomes
Endoplasmic Reticulum
Endosome
Golgi
Lipid Particles
Mitochondria
None
Nuclear Periphery
Nucleolus
Nucleus
Peroxisomes
Punctate Nuclear
Vacuole
Vacuole Periphery

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 20.9435 14.3622 20.5535 24.2286 18.2267 16.0573 23.9866 23.8027 29.3262 23.1233
Translational Efficiency 0.4619 0.735 0.6052 0.5606 0.559 0.5848 0.3872 0.4248 0.3726 0.3785

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description F-box protein containing five copies of the WD40 motif; controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus; dissociation of Met30p from SCF complex in response to cadmium stress is regulated by Cdc48p
Localization
Cell Percentages nucleus (30%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Met30

Met30


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Met30-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available