Standard name
Human Ortholog
Description Phospholipid translocase (flippase), role in phospholipid asymmetry of plasma membrane; involved in endocytosis, vacuolar biogenesis and Golgi to ER vesicle-mediated transport; localizes to endosomes and the Golgi apparatus
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.09 0.08 0 – 0 – – – – – – – – – – – – – 0 0.05 0 0 0 0
Bud 0 0 0 – 0 – – – – – – – – – – – – – 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 – – – – – – – – – – – – – 0 0 0 0 0 0
Bud Site 0 0 0 – 0 – – – – – – – – – – – – – – – – – – –
Cell Periphery 0 0 0 – 0 – – – – – – – – – – – – – 0 0 0 0 0 0
Cytoplasm 0.29 0.54 0.79 – 0.91 – – – – – – – – – – – – – 0 0.13 0.2 0.07 0.13 0.13
Endoplasmic Reticulum 0.58 0.44 0.05 – 0.05 – – – – – – – – – – – – – 0.54 0.44 0.39 0.66 0.57 0.52
Endosome 0.12 0 0.08 – 0.05 – – – – – – – – – – – – – 0.15 0.13 0.12 0.07 0.09 0.15
Golgi 0 0 0.05 – 0 – – – – – – – – – – – – – 0.14 0.1 0.06 0 0.08 0.05
Mitochondria 0 0 0 – 0 – – – – – – – – – – – – – 0 0.05 0 0 0 0
Nucleus 0 0 0 – 0.05 – – – – – – – – – – – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 – – – – – – – – – – – – – 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 – – – – – – – – – – – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 – – – – – – – – – – – – – 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 – – – – – – – – – – – – – 0 0 0 0 0 0
Vac/Vac Membrane 0.09 0 0.1 – 0.05 – – – – – – – – – – – – – 0.05 0.06 0.09 0 0.07 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 16 17 0 – 0 – – – – – – – – – – – – – 7 13 2 0 1 0
Bud 1 5 0 – 0 – – – – – – – – – – – – – 0 1 1 0 0 0
Bud Neck 0 0 0 – 0 – – – – – – – – – – – – – 1 2 0 0 0 0
Bud Site 0 0 0 – 0 – – – – – – – – – – – – – – – – – – –
Cell Periphery 1 0 0 – 0 – – – – – – – – – – – – – 0 0 0 0 0 0
Cytoplasm 54 117 48 – 20 – – – – – – – – – – – – – 7 32 15 2 6 4
Endoplasmic Reticulum 108 95 3 – 1 – – – – – – – – – – – – – 118 111 31 19 29 19
Endosome 22 6 5 – 1 – – – – – – – – – – – – – 32 32 9 2 4 5
Golgi 8 3 3 – 0 – – – – – – – – – – – – – 30 24 4 1 4 1
Mitochondria 1 8 0 – 0 – – – – – – – – – – – – – 4 13 1 0 0 0
Nucleus 0 1 0 – 1 – – – – – – – – – – – – – 0 1 0 0 0 0
Nuclear Periphery 0 0 0 – 0 – – – – – – – – – – – – – 1 0 1 0 0 0
Nucleolus 0 0 1 – 0 – – – – – – – – – – – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 – – – – – – – – – – – – – 1 0 0 0 0 0
SpindlePole 2 0 0 – 0 – – – – – – – – – – – – – 1 1 0 0 0 0
Vac/Vac Membrane 17 4 6 – 1 – – – – – – – – – – – – – 10 15 7 0 3 3
Unique Cell Count 187 217 61 22 219 255 80 29 52 38
Labelled Cell Count 230 256 66 24 219 255 80 29 52 38


Early Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.1 9.4 9.5 – 9.8 – – – – – – – – – – – – – – – 12.7
Std Deviation (1e-4) 1.3 1.9 2.0 – 2.5 – – – – – – – – – – – – – – – 3.6
Intensity Change (Log2) – – – – 0.05 – – – – – – – – – – – – – – – 0.42


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches – 0 – – – – – – – – – – – – –
Bud – 0 – – – – – – – – – – – – –
Bud Neck – 0 – – – – – – – – – – – – –
Bud Site – 0 – – – – – – – – – – – – –
Cell Periphery – 0 – – – – – – – – – – – – –
Cytoplasm – 1.3 – – – – – – – – – – – – –
Endoplasmic Reticulum – 0 – – – – – – – – – – – – –
Endosome – 0 – – – – – – – – – – – – –
Golgi – 0 – – – – – – – – – – – – –
Mitochondria – 0 – – – – – – – – – – – – –
Nucleus – 0 – – – – – – – – – – – – –
Nuclear Periphery – 0 – – – – – – – – – – – – –
Nucleolus – 0 – – – – – – – – – – – – –
Peroxisomes – 0 – – – – – – – – – – – – –
SpindlePole – 0 – – – – – – – – – – – – –
Vacuole – 0 – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.1949 7.3841 6.2553 5.8015 5.7244 6.6336 7.4897 8.4634 7.8979 7.7396 7.345 8.0051 7.6726 8.7213 8.2042 7.9694 7.6876 8.0588
Actin 0.0515 0.0032 0.0207 0.0597 0.0705 0.0062 0.0273 0.0025 0.0238 0.0025 0.0225 0.0086 0.016 0.0019 0.0059 0.003 0.0025 0.0031
Bud 0.0008 0.0002 0.0004 0.0004 0.0018 0.0002 0.0003 0.0002 0.0009 0.0003 0.002 0.0035 0.0005 0.0001 0.0003 0.0001 0.0001 0.0002
Bud Neck 0.0012 0.0004 0.001 0.0013 0.0004 0.0007 0.0011 0.0004 0.0015 0.0005 0.0007 0.0008 0.001 0.0003 0.0011 0.0005 0.0033 0.0021
Bud Periphery 0.001 0.0001 0.0005 0.0005 0.002 0.0003 0.0004 0.0001 0.001 0.0002 0.0029 0.0021 0.0007 0.0001 0.0003 0.0002 0.0001 0.0003
Bud Site 0.003 0.0046 0.0042 0.0017 0.0038 0.0002 0.0019 0.0016 0.0052 0.0003 0.0028 0.0007 0.003 0.0008 0.0047 0.0001 0.0002 0.0002
Cell Periphery 0.0005 0.0004 0.0004 0.0003 0.0001 0.0002 0.0006 0.0006 0.0005 0.0002 0.0003 0.0004 0.0006 0.0005 0.0005 0.0006 0.0003 0.0003
Cytoplasm 0.077 0.1672 0.0765 0.0398 0.0122 0.0867 0.0368 0.1259 0.0541 0.0577 0.0242 0.056 0.0517 0.0765 0.0661 0.0759 0.0185 0.077
Cytoplasmic Foci 0.0567 0.0655 0.0721 0.0863 0.0651 0.0598 0.0526 0.0949 0.0848 0.1022 0.0681 0.0533 0.064 0.0947 0.0959 0.1181 0.0653 0.0637
Eisosomes 0.0005 0 0.0003 0.0003 0.0001 0 0.0002 0 0.0002 0 0.0001 0 0.0002 0 0 0 0 0
Endoplasmic Reticulum 0.302 0.3012 0.2973 0.2469 0.1172 0.3362 0.2651 0.2079 0.209 0.088 0.0622 0.2787 0.2724 0.2413 0.2598 0.1938 0.2184 0.273
Endosome 0.2415 0.3057 0.3538 0.3447 0.3559 0.3383 0.3397 0.3721 0.3571 0.4363 0.4724 0.361 0.311 0.3514 0.3247 0.3931 0.4175 0.3613
Golgi 0.0626 0.0569 0.0715 0.0982 0.1008 0.0765 0.081 0.0841 0.149 0.1762 0.1491 0.1144 0.0989 0.1015 0.1245 0.1746 0.1574 0.1225
Lipid Particles 0.0393 0.0486 0.0379 0.0596 0.1432 0.0296 0.0975 0.0731 0.0557 0.0589 0.0922 0.0582 0.0691 0.0886 0.052 0.0182 0.0842 0.0482
Mitochondria 0.015 0.0023 0.0114 0.0029 0.0079 0.0078 0.0084 0.0048 0.0122 0.0325 0.0159 0.0096 0.0244 0.0129 0.0244 0.0071 0.0045 0.0151
None 0.0121 0.0012 0.0026 0.002 0.0006 0.0013 0.0023 0.0041 0.0014 0.0029 0.0021 0.0047 0.002 0.0015 0.0018 0.0009 0.0003 0.0013
Nuclear Periphery 0.071 0.0233 0.0149 0.0294 0.0836 0.0357 0.0367 0.0092 0.0114 0.0057 0.0462 0.0203 0.0357 0.0079 0.0101 0.0027 0.0076 0.0112
Nucleolus 0.0019 0 0.0004 0.0001 0.0001 0 0.0003 0.0001 0.0002 0.0001 0.0009 0.0006 0.0002 0.0001 0.0001 0 0 0.0002
Nucleus 0.0134 0.0019 0.0017 0.0012 0.0009 0.0016 0.0082 0.001 0.0012 0.0004 0.0092 0.0026 0.0125 0.0006 0.0007 0.0003 0.0003 0.0013
Peroxisomes 0.0061 0.0008 0.0121 0.0062 0.022 0.0013 0.0138 0.0015 0.0111 0.0201 0.0034 0.0019 0.0051 0.0022 0.0105 0.0018 0.0009 0.0012
Punctate Nuclear 0.0237 0.0011 0.0023 0.0042 0.0025 0.0011 0.0022 0.0011 0.0025 0.002 0.0051 0.0033 0.0016 0.0013 0.0012 0.0003 0.0004 0.0016
Vacuole 0.0122 0.0123 0.0133 0.0103 0.0051 0.0102 0.0178 0.0123 0.0131 0.0078 0.01 0.0118 0.0207 0.0128 0.0117 0.0052 0.0128 0.0104
Vacuole Periphery 0.007 0.003 0.0046 0.0041 0.0041 0.0059 0.006 0.0024 0.0042 0.0052 0.0077 0.0076 0.0089 0.0029 0.0038 0.0033 0.0054 0.0058

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.8883 13.6997 17.5783 23.2195 15.1092 12.7638 23.3748 27.0731 23.7904 23.7468
Translational Efficiency 0.7107 0.7268 0.7173 0.4119 0.6163 0.9335 0.5142 0.4184 0.4753 0.5332

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Phospholipid translocase (flippase), role in phospholipid asymmetry of plasma membrane; involved in endocytosis, vacuolar biogenesis and Golgi to ER vesicle-mediated transport; localizes to endosomes and the Golgi apparatus
Localization
Cell Percentages ER (69%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Neo1

Neo1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Neo1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available