ORF | |
---|---|
Human Ortholog | |
Description | Putative protein of unknown function |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 3 | 1 | 2 | – | 0 | 0 | – | – | 0 | 4 | 0 | 0 | 0 | 2 | 1 |
Bud | 1 | 1 | 0 | 0 | 3 | 0 | 0 | 4 | 4 | 2 | 4 | 4 | – | 0 | 0 | – | – | 0 | 1 | 1 | 0 | 0 | 2 | 1 |
Bud Neck | 2 | 1 | 0 | 0 | 0 | 1 | 2 | 5 | 0 | 1 | 0 | 2 | – | 0 | 0 | – | – | 0 | 1 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | 0 | – | – | – | – | – | – |
Cell Periphery | 6 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 2 | 1 | 1 | – | 5 | 3 | – | – | 1 | 0 | 1 | 0 | 0 | 0 | 0 |
Cytoplasm | 330 | 221 | 20 | 58 | 138 | 145 | 208 | 201 | 41 | 150 | 97 | 125 | – | 140 | 135 | – | – | 35 | 284 | 199 | 33 | 60 | 118 | 130 |
Endoplasmic Reticulum | 1 | 3 | 0 | 0 | 0 | 4 | 1 | 2 | 0 | 2 | 2 | 3 | – | 0 | 3 | – | – | 5 | 1 | 1 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 2 | 0 | 1 | 0 | 0 | – | 0 | 0 | – | – | 1 | 0 | 1 | 0 | 0 | 0 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | 0 | 1 | 1 | 0 | 0 | 0 | 0 |
Mitochondria | 11 | 2 | 0 | 8 | 4 | 6 | 24 | 18 | 32 | 44 | 106 | 85 | – | 0 | 0 | – | – | 2 | 30 | 15 | 11 | 0 | 3 | 3 |
Nucleus | 0 | 0 | 0 | 1 | 2 | 3 | 0 | 2 | 0 | 0 | 1 | 1 | – | 0 | 0 | – | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 4 | 0 | 2 | 1 | 3 | – | 0 | 0 | – | – | 0 | 2 | 1 | 0 | 0 | 1 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 2 | 2 | 4 | 2 | 1 | – | 0 | 0 | – | – | 2 | 3 | 2 | 1 | 0 | 1 | 0 |
Unique Cell Count | 336 | 225 | 20 | 61 | 142 | 154 | 224 | 230 | 59 | 187 | 170 | 187 | 140 | 138 | 41 | 337 | 233 | 51 | 65 | 137 | 142 | |||
Labelled Cell Count | 351 | 230 | 20 | 68 | 151 | 161 | 241 | 244 | 80 | 211 | 216 | 227 | 145 | 141 | 48 | 337 | 233 | 51 | 65 | 137 | 142 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.7 | 4.6 | 4.5 | 4.6 | 6.1 | 4.6 | 4.9 | 5.1 | 4.0 | 4.5 | 4.0 | 4.1 | – | 5.7 | 6.0 | – | – | 9.1 | – | 5.1 | 5.8 |
Std Deviation (1e-4) | 0.4 | 0.9 | 0.6 | 1.0 | 3.8 | 1.0 | 1.7 | 1.6 | 1.0 | 1.1 | 1.3 | 1.3 | – | 1.1 | 1.3 | – | – | 2.2 | – | 1.4 | 1.5 |
Intensity Change (Log2) | – | – | – | 0.02 | 0.44 | 0.04 | 0.11 | 0.16 | -0.18 | -0.02 | -0.18 | -0.14 | – | 0.33 | 0.4 | – | – | 1.01 | – | 0.18 | 0.35 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.3124 | -0.2559 | -0.2614 | -0.627 | -0.0813 | -0.275 | -0.5111 | -0.044 | -0.3212 | -0.5542 | -0.5399 | -0.2541 | -1.1949 | -1.2364 | -1.0464 | -1.06 | -0.1636 | -1.22 |
Actin | 0.0458 | 0.0061 | 0.0086 | 0.0108 | 0.0393 | 0.0118 | 0.0349 | 0.0025 | 0.0323 | 0.0034 | 0.0001 | 0.0007 | 0.016 | 0.0006 | 0.0092 | 0.0069 | 0.0193 | 0.0054 |
Bud | 0.0031 | 0.0001 | 0.0005 | 0.0162 | 0.0002 | 0.0005 | 0.0013 | 0.004 | 0.0015 | 0.0003 | 0 | 0.0002 | 0.0022 | 0.0007 | 0.0013 | 0.0007 | 0.0008 | 0.0003 |
Bud Neck | 0.0138 | 0.0001 | 0.0092 | 0.0005 | 0.0001 | 0.013 | 0.0009 | 0.0006 | 0.0005 | 0.0004 | 0.0001 | 0.0008 | 0.0129 | 0.0001 | 0.0016 | 0.0111 | 0.0007 | 0.0005 |
Bud Periphery | 0.0035 | 0.0002 | 0.0009 | 0.0097 | 0.0002 | 0.0013 | 0.0026 | 0.0044 | 0.0022 | 0.0003 | 0.0001 | 0.0002 | 0.0034 | 0.0009 | 0.0014 | 0.0012 | 0.0021 | 0.0008 |
Bud Site | 0.007 | 0.0007 | 0.0036 | 0.0005 | 0.0012 | 0.0012 | 0.0053 | 0.0128 | 0.0079 | 0.0003 | 0 | 0.0003 | 0.0134 | 0.0071 | 0.0104 | 0.0077 | 0.0025 | 0.0001 |
Cell Periphery | 0.0007 | 0.0002 | 0.0004 | 0.0003 | 0.0001 | 0.0002 | 0.0006 | 0.0005 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0013 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0001 |
Cytoplasm | 0.0938 | 0.1635 | 0.1988 | 0.1954 | 0.1773 | 0.2311 | 0.1158 | 0.2422 | 0.1652 | 0.3657 | 0.3281 | 0.298 | 0.1256 | 0.2396 | 0.1595 | 0.1925 | 0.1705 | 0.3655 |
Cytoplasmic Foci | 0.0545 | 0.0085 | 0.016 | 0.0071 | 0.0207 | 0.0133 | 0.029 | 0.0125 | 0.0196 | 0.03 | 0.0042 | 0.0229 | 0.0167 | 0.0106 | 0.0193 | 0.0216 | 0.0693 | 0.0129 |
Eisosomes | 0.0007 | 0.0004 | 0.0004 | 0.0003 | 0.0002 | 0.0003 | 0.0005 | 0.0002 | 0.0004 | 0.0002 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0002 |
Endoplasmic Reticulum | 0.0056 | 0.0021 | 0.0065 | 0.0011 | 0.0124 | 0.0027 | 0.0051 | 0.0024 | 0.0023 | 0.0027 | 0.002 | 0.0021 | 0.0026 | 0.0022 | 0.0021 | 0.0085 | 0.0016 | 0.0018 |
Endosome | 0.0242 | 0.0023 | 0.0115 | 0.006 | 0.0134 | 0.0103 | 0.0113 | 0.008 | 0.0056 | 0.0353 | 0.0017 | 0.0062 | 0.026 | 0.0038 | 0.0103 | 0.0104 | 0.0197 | 0.0117 |
Golgi | 0.0126 | 0.0005 | 0.0041 | 0.0065 | 0.0034 | 0.0049 | 0.0031 | 0.0007 | 0.0019 | 0.0007 | 0.0001 | 0.0006 | 0.0045 | 0.0004 | 0.0045 | 0.0036 | 0.0124 | 0.0023 |
Lipid Particles | 0.0146 | 0.0011 | 0.0037 | 0.005 | 0.0047 | 0.0033 | 0.0118 | 0.0007 | 0.0026 | 0.0007 | 0.0001 | 0.0008 | 0.0077 | 0.0009 | 0.0058 | 0.0023 | 0.012 | 0.001 |
Mitochondria | 0.0113 | 0.0004 | 0.0145 | 0.0219 | 0.0008 | 0.0182 | 0.0054 | 0.0007 | 0.0016 | 0.0006 | 0.0002 | 0.0006 | 0.0023 | 0.0003 | 0.0029 | 0.0008 | 0.0493 | 0.0048 |
None | 0.6335 | 0.8102 | 0.694 | 0.7133 | 0.691 | 0.677 | 0.7456 | 0.6979 | 0.7414 | 0.5454 | 0.6617 | 0.6516 | 0.7338 | 0.7289 | 0.7581 | 0.7207 | 0.5204 | 0.5884 |
Nuclear Periphery | 0.0081 | 0.0005 | 0.0049 | 0.0007 | 0.0017 | 0.0022 | 0.0044 | 0.0013 | 0.002 | 0.001 | 0.0003 | 0.0012 | 0.0028 | 0.0005 | 0.0011 | 0.0021 | 0.0356 | 0.0006 |
Nucleolus | 0.0012 | 0.0002 | 0.0008 | 0.0002 | 0.0001 | 0.001 | 0.0008 | 0.0009 | 0.0004 | 0.0003 | 0 | 0.0005 | 0.0008 | 0.0001 | 0.0005 | 0.0002 | 0.0007 | 0.0001 |
Nucleus | 0.0056 | 0.0005 | 0.0019 | 0.0008 | 0.0005 | 0.0016 | 0.0017 | 0.002 | 0.0016 | 0.0012 | 0.0003 | 0.0022 | 0.0018 | 0.0007 | 0.0009 | 0.001 | 0.0149 | 0.0006 |
Peroxisomes | 0.0218 | 0.0009 | 0.0121 | 0.0012 | 0.026 | 0.001 | 0.0094 | 0.001 | 0.0038 | 0.0007 | 0 | 0.0055 | 0.0137 | 0.0002 | 0.0059 | 0.0042 | 0.0469 | 0.0005 |
Punctate Nuclear | 0.0343 | 0.0005 | 0.0017 | 0.0005 | 0.0054 | 0.0017 | 0.0081 | 0.0026 | 0.0037 | 0.0022 | 0.0001 | 0.0039 | 0.004 | 0.0006 | 0.0026 | 0.0008 | 0.0173 | 0.0005 |
Vacuole | 0.0028 | 0.001 | 0.0029 | 0.0013 | 0.0011 | 0.0025 | 0.0018 | 0.0018 | 0.0026 | 0.0069 | 0.0009 | 0.0013 | 0.007 | 0.0012 | 0.0018 | 0.003 | 0.0014 | 0.0014 |
Vacuole Periphery | 0.0013 | 0.0001 | 0.0031 | 0.0006 | 0.0002 | 0.0008 | 0.0008 | 0.0003 | 0.0005 | 0.0013 | 0.0001 | 0.0003 | 0.0013 | 0.0002 | 0.0004 | 0.0007 | 0.0021 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 2.3427 | 2.6178 | 5.0505 | 5.8142 | 1.139 | 7.0242 | 5.6907 | 10.3613 | 9.8845 | 6.1029 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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